Vibrio phage V07
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 516 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H0X7Z2|A0A6H0X7Z2_9CAUD Uncharacterized protein OS=Vibrio phage V07 OX=2724326 GN=GCAPEGMB_00164 PE=4 SV=1
MM1 pKa = 7.6 IVLQHH6 pKa = 5.85 EE7 pKa = 4.25 TDD9 pKa = 3.84 GYY11 pKa = 10.16 IVQIGCDD18 pKa = 3.35 ICCTPDD24 pKa = 2.95 IDD26 pKa = 3.94 KK27 pKa = 11.35 ASMFEE32 pKa = 4.36 TVGVAQEE39 pKa = 5.99 FKK41 pKa = 10.83 DD42 pKa = 5.27 QWDD45 pKa = 3.41 LWDD48 pKa = 3.46 WEE50 pKa = 4.2 VRR52 pKa = 11.84 EE53 pKa = 4.16 VGLL56 pKa = 4.91
Molecular weight: 6.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A6H0X8K6|A0A6H0X8K6_9CAUD DNA_LIGASE_A3 domain-containing protein OS=Vibrio phage V07 OX=2724326 GN=GCAPEGMB_00417 PE=4 SV=1
MM1 pKa = 7.41 FMRR4 pKa = 11.84 GSLEE8 pKa = 3.89 HH9 pKa = 7.4 DD10 pKa = 3.81 VFCTIVTLNRR20 pKa = 11.84 FTYY23 pKa = 10.6 SEE25 pKa = 4.61 LSCNMGVSSARR36 pKa = 11.84 IRR38 pKa = 11.84 RR39 pKa = 11.84 AIKK42 pKa = 9.63 SLRR45 pKa = 11.84 QKK47 pKa = 10.89 GVLIQTTGGGKK58 pKa = 10.08 GSGMEE63 pKa = 3.65 QEE65 pKa = 5.11 RR66 pKa = 11.84 FLEE69 pKa = 4.52 YY70 pKa = 10.13 KK71 pKa = 10.43 GHH73 pKa = 7.22 LPPKK77 pKa = 9.45 QKK79 pKa = 10.36 RR80 pKa = 11.84 GLHH83 pKa = 5.77 GNPNN87 pKa = 3.44
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.487
IPC_protein 9.75
Toseland 10.467
ProMoST 10.028
Dawson 10.57
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 10.979
Grimsley 10.613
Solomon 10.628
Lehninger 10.613
Nozaki 10.467
DTASelect 10.204
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.496
Patrickios 10.76
IPC_peptide 10.643
IPC2_peptide 9.136
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
516
0
516
105896
41
1256
205.2
23.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.437 ± 0.139
1.195 ± 0.06
6.606 ± 0.101
7.374 ± 0.13
4.127 ± 0.076
6.432 ± 0.134
2.257 ± 0.063
6.217 ± 0.094
6.616 ± 0.13
7.527 ± 0.112
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.814 ± 0.077
5.107 ± 0.083
3.413 ± 0.081
3.628 ± 0.109
4.927 ± 0.076
5.869 ± 0.119
6.09 ± 0.152
6.876 ± 0.093
1.46 ± 0.045
4.029 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here