Colwellia sp. PAMC 21821

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia; unclassified Colwellia

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0KSI6|A0A1V0KSI6_9GAMM PspC domain-containing protein OS=Colwellia sp. PAMC 21821 OX=1816219 GN=A3Q33_10585 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.25KK3 pKa = 9.56IALAVTLASILSTNVQADD21 pKa = 3.27ALGIYY26 pKa = 10.24LGGQIWDD33 pKa = 3.81NQASGTFGDD42 pKa = 4.94GSSQVDD48 pKa = 3.79FNLVDD53 pKa = 3.64EE54 pKa = 4.83KK55 pKa = 11.4QNSFFIAFEE64 pKa = 4.42HH65 pKa = 6.54PLPLIPNIRR74 pKa = 11.84IASTALEE81 pKa = 4.28TKK83 pKa = 10.4GNKK86 pKa = 7.89TLNTEE91 pKa = 4.4FEE93 pKa = 4.59FEE95 pKa = 4.72GEE97 pKa = 4.3TFSQGTNVNADD108 pKa = 3.67FNVSYY113 pKa = 11.38VDD115 pKa = 3.23YY116 pKa = 9.57TLYY119 pKa = 11.34YY120 pKa = 10.64EE121 pKa = 5.79LFDD124 pKa = 4.27NDD126 pKa = 4.73LVSFDD131 pKa = 3.69IGLTGRR137 pKa = 11.84DD138 pKa = 3.22FDD140 pKa = 5.19GDD142 pKa = 3.99VTVSASSGANSQSGSVSVTDD162 pKa = 3.83IVPMLYY168 pKa = 10.61ARR170 pKa = 11.84TNVGLPLTGFNLYY183 pKa = 10.53AQGNFLSIDD192 pKa = 3.26DD193 pKa = 4.06HH194 pKa = 5.88TLYY197 pKa = 10.61DD198 pKa = 3.82YY199 pKa = 11.18EE200 pKa = 4.58VGVSYY205 pKa = 10.76EE206 pKa = 4.62LIDD209 pKa = 4.14NLAIDD214 pKa = 3.71VNINVGYY221 pKa = 10.1RR222 pKa = 11.84AVKK225 pKa = 10.76LEE227 pKa = 4.46LDD229 pKa = 4.55DD230 pKa = 6.81LNDD233 pKa = 3.98LYY235 pKa = 11.43TNIEE239 pKa = 3.85FDD241 pKa = 4.09GVFLGTTIHH250 pKa = 6.76FF251 pKa = 4.43

Molecular weight: 27.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0KRE6|A0A1V0KRE6_9GAMM Ribonuclease P protein component OS=Colwellia sp. PAMC 21821 OX=1816219 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21NGRR28 pKa = 11.84AVISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3841

0

3841

1307913

37

4402

340.5

37.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.559 ± 0.044

0.981 ± 0.014

5.625 ± 0.048

5.879 ± 0.035

4.357 ± 0.027

6.47 ± 0.036

2.141 ± 0.02

7.152 ± 0.037

5.861 ± 0.04

10.128 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.023

5.123 ± 0.041

3.617 ± 0.021

4.451 ± 0.031

3.877 ± 0.032

6.91 ± 0.042

5.601 ± 0.04

6.63 ± 0.033

1.144 ± 0.015

3.106 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski