Amino acid dipepetide frequency for Colwellia sp. PAMC 21821

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.086AlaAla: 7.086 ± 0.098	0.951AlaCys: 0.951 ± 0.031	4.86AlaAsp: 4.86 ± 0.076	5.409AlaGlu: 5.409 ± 0.073	3.527AlaPhe: 3.527 ± 0.059	5.739AlaGly: 5.739 ± 0.087	1.505AlaHis: 1.505 ± 0.035	6.782AlaIle: 6.782 ± 0.085	5.513AlaLys: 5.513 ± 0.075	9.433AlaLeu: 9.433 ± 0.098	2.409AlaMet: 2.409 ± 0.039	4.246AlaAsn: 4.246 ± 0.07	2.717AlaPro: 2.717 ± 0.054	3.804AlaGln: 3.804 ± 0.061	3.051AlaArg: 3.051 ± 0.057	5.379AlaSer: 5.379 ± 0.075	4.55AlaThr: 4.55 ± 0.084	5.371AlaVal: 5.371 ± 0.069	0.909AlaTrp: 0.909 ± 0.026	2.345AlaTyr: 2.345 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.717CysAla: 0.717 ± 0.024	0.155CysCys: 0.155 ± 0.012	0.571CysAsp: 0.571 ± 0.021	0.541CysGlu: 0.541 ± 0.022	0.489CysPhe: 0.489 ± 0.022	0.753CysGly: 0.753 ± 0.029	0.316CysHis: 0.316 ± 0.017	0.636CysIle: 0.636 ± 0.022	0.434CysLys: 0.434 ± 0.021	0.979CysLeu: 0.979 ± 0.026	0.189CysMet: 0.189 ± 0.013	0.378CysAsn: 0.378 ± 0.016	0.438CysPro: 0.438 ± 0.022	0.573CysGln: 0.573 ± 0.024	0.417CysArg: 0.417 ± 0.02	0.711CysSer: 0.711 ± 0.022	0.484CysThr: 0.484 ± 0.023	0.583CysVal: 0.583 ± 0.021	0.117CysTrp: 0.117 ± 0.011	0.33CysTyr: 0.33 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.297AspAla: 4.297 ± 0.067	0.523AspCys: 0.523 ± 0.018	3.429AspAsp: 3.429 ± 0.084	3.788AspGlu: 3.788 ± 0.056	2.731AspPhe: 2.731 ± 0.046	3.736AspGly: 3.736 ± 0.107	1.093AspHis: 1.093 ± 0.03	4.794AspIle: 4.794 ± 0.078	3.631AspLys: 3.631 ± 0.057	5.174AspLeu: 5.174 ± 0.065	1.382AspMet: 1.382 ± 0.035	3.324AspAsn: 3.324 ± 0.105	1.824AspPro: 1.824 ± 0.04	1.839AspGln: 1.839 ± 0.044	1.905AspArg: 1.905 ± 0.043	3.396AspSer: 3.396 ± 0.076	2.778AspThr: 2.778 ± 0.06	3.832AspVal: 3.832 ± 0.06	0.716AspTrp: 0.716 ± 0.024	2.06AspTyr: 2.06 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	4.561GluAla: 4.561 ± 0.072	0.423GluCys: 0.423 ± 0.019	2.755GluAsp: 2.755 ± 0.046	3.111GluGlu: 3.111 ± 0.061	2.542GluPhe: 2.542 ± 0.047	3.002GluGly: 3.002 ± 0.053	1.625GluHis: 1.625 ± 0.041	4.165GluIle: 4.165 ± 0.067	4.098GluLys: 4.098 ± 0.067	6.688GluLeu: 6.688 ± 0.075	1.427GluMet: 1.427 ± 0.038	3.408GluAsn: 3.408 ± 0.05	1.694GluPro: 1.694 ± 0.041	3.97GluGln: 3.97 ± 0.074	2.642GluArg: 2.642 ± 0.049	3.568GluSer: 3.568 ± 0.058	2.928GluThr: 2.928 ± 0.05	3.873GluVal: 3.873 ± 0.067	0.535GluTrp: 0.535 ± 0.021	1.777GluTyr: 1.777 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.577PheAla: 3.577 ± 0.061	0.485PheCys: 0.485 ± 0.021	2.99PheAsp: 2.99 ± 0.053	2.576PheGlu: 2.576 ± 0.051	1.859PhePhe: 1.859 ± 0.045	3.153PheGly: 3.153 ± 0.055	0.853PheHis: 0.853 ± 0.027	3.411PheIle: 3.411 ± 0.066	2.314PheLys: 2.314 ± 0.045	3.423PheLeu: 3.423 ± 0.062	0.947PheMet: 0.947 ± 0.031	2.554PheAsn: 2.554 ± 0.05	1.4PhePro: 1.4 ± 0.037	1.347PheGln: 1.347 ± 0.036	1.361PheArg: 1.361 ± 0.031	3.818PheSer: 3.818 ± 0.065	2.812PheThr: 2.812 ± 0.047	2.689PheVal: 2.689 ± 0.049	0.518PheTrp: 0.518 ± 0.02	1.486PheTyr: 1.486 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.014GlyAla: 5.014 ± 0.074	0.82GlyCys: 0.82 ± 0.026	3.714GlyAsp: 3.714 ± 0.077	3.913GlyGlu: 3.913 ± 0.059	3.422GlyPhe: 3.422 ± 0.064	4.48GlyGly: 4.48 ± 0.082	1.443GlyHis: 1.443 ± 0.034	4.777GlyIle: 4.777 ± 0.077	4.05GlyLys: 4.05 ± 0.066	6.445GlyLeu: 6.445 ± 0.082	1.704GlyMet: 1.704 ± 0.035	2.874GlyAsn: 2.874 ± 0.06	1.548GlyPro: 1.548 ± 0.037	2.532GlyGln: 2.532 ± 0.042	2.645GlyArg: 2.645 ± 0.052	4.03GlySer: 4.03 ± 0.069	3.268GlyThr: 3.268 ± 0.063	4.744GlyVal: 4.744 ± 0.068	0.835GlyTrp: 0.835 ± 0.026	2.438GlyTyr: 2.438 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.567HisAla: 1.567 ± 0.035	0.326HisCys: 0.326 ± 0.016	1.122HisAsp: 1.122 ± 0.03	1.073HisGlu: 1.073 ± 0.031	1.101HisPhe: 1.101 ± 0.029	1.528HisGly: 1.528 ± 0.042	0.654HisHis: 0.654 ± 0.023	1.479HisIle: 1.479 ± 0.036	1.119HisLys: 1.119 ± 0.033	2.082HisLeu: 2.082 ± 0.05	0.479HisMet: 0.479 ± 0.021	0.997HisAsn: 0.997 ± 0.031	0.99HisPro: 0.99 ± 0.033	1.236HisGln: 1.236 ± 0.036	0.875HisArg: 0.875 ± 0.03	1.386HisSer: 1.386 ± 0.037	0.953HisThr: 0.953 ± 0.028	1.252HisVal: 1.252 ± 0.03	0.35HisTrp: 0.35 ± 0.019	0.846HisTyr: 0.846 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.163IleAla: 7.163 ± 0.068	0.722IleCys: 0.722 ± 0.025	4.772IleAsp: 4.772 ± 0.068	4.961IleGlu: 4.961 ± 0.069	2.788IlePhe: 2.788 ± 0.057	4.681IleGly: 4.681 ± 0.077	1.263IleHis: 1.263 ± 0.033	4.795IleIle: 4.795 ± 0.071	4.422IleLys: 4.422 ± 0.066	5.757IleLeu: 5.757 ± 0.077	1.413IleMet: 1.413 ± 0.033	4.145IleAsn: 4.145 ± 0.055	2.583IlePro: 2.583 ± 0.05	2.255IleGln: 2.255 ± 0.043	2.606IleArg: 2.606 ± 0.047	5.523IleSer: 5.523 ± 0.072	4.564IleThr: 4.564 ± 0.071	4.409IleVal: 4.409 ± 0.06	0.688IleTrp: 0.688 ± 0.027	2.009IleTyr: 2.009 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	5.263LysAla: 5.263 ± 0.075	0.372LysCys: 0.372 ± 0.019	2.895LysAsp: 2.895 ± 0.051	3.197LysGlu: 3.197 ± 0.054	2.044LysPhe: 2.044 ± 0.044	3.223LysGly: 3.223 ± 0.06	1.395LysHis: 1.395 ± 0.034	3.964LysIle: 3.964 ± 0.07	3.596LysLys: 3.596 ± 0.063	6.016LysLeu: 6.016 ± 0.077	1.493LysMet: 1.493 ± 0.036	3.261LysAsn: 3.261 ± 0.056	2.347LysPro: 2.347 ± 0.044	3.085LysGln: 3.085 ± 0.061	2.551LysArg: 2.551 ± 0.05	4.09LysSer: 4.09 ± 0.056	3.313LysThr: 3.313 ± 0.057	4.198LysVal: 4.198 ± 0.055	0.562LysTrp: 0.562 ± 0.021	1.744LysTyr: 1.744 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	10.03LeuAla: 10.03 ± 0.1	0.939LeuCys: 0.939 ± 0.029	5.422LeuAsp: 5.422 ± 0.073	5.342LeuGlu: 5.342 ± 0.068	4.209LeuPhe: 4.209 ± 0.082	6.378LeuGly: 6.378 ± 0.08	1.934LeuHis: 1.934 ± 0.043	6.857LeuIle: 6.857 ± 0.099	5.632LeuLys: 5.632 ± 0.076	10.297LeuLeu: 10.297 ± 0.148	2.567LeuMet: 2.567 ± 0.049	5.129LeuAsn: 5.129 ± 0.065	4.562LeuPro: 4.562 ± 0.055	3.46LeuGln: 3.46 ± 0.062	3.747LeuArg: 3.747 ± 0.069	8.127LeuSer: 8.127 ± 0.088	6.519LeuThr: 6.519 ± 0.079	6.488LeuVal: 6.488 ± 0.089	0.979LeuTrp: 0.979 ± 0.032	2.659LeuTyr: 2.659 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.363MetAla: 2.363 ± 0.047	0.2MetCys: 0.2 ± 0.013	1.057MetAsp: 1.057 ± 0.033	1.031MetGlu: 1.031 ± 0.03	0.915MetPhe: 0.915 ± 0.029	1.512MetGly: 1.512 ± 0.039	0.478MetHis: 0.478 ± 0.018	1.447MetIle: 1.447 ± 0.036	1.423MetLys: 1.423 ± 0.031	2.611MetLeu: 2.611 ± 0.049	0.654MetMet: 0.654 ± 0.024	1.09MetAsn: 1.09 ± 0.03	1.227MetPro: 1.227 ± 0.032	1.095MetGln: 1.095 ± 0.028	1.023MetArg: 1.023 ± 0.032	1.883MetSer: 1.883 ± 0.039	1.555MetThr: 1.555 ± 0.033	1.603MetVal: 1.603 ± 0.037	0.229MetTrp: 0.229 ± 0.014	0.492MetTyr: 0.492 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	4.064AsnAla: 4.064 ± 0.083	0.515AsnCys: 0.515 ± 0.021	2.899AsnAsp: 2.899 ± 0.059	2.888AsnGlu: 2.888 ± 0.044	2.157AsnPhe: 2.157 ± 0.049	3.431AsnGly: 3.431 ± 0.066	1.008AsnHis: 1.008 ± 0.028	4.061AsnIle: 4.061 ± 0.063	3.386AsnLys: 3.386 ± 0.055	4.587AsnLeu: 4.587 ± 0.072	1.093AsnMet: 1.093 ± 0.027	3.084AsnAsn: 3.084 ± 0.07	1.948AsnPro: 1.948 ± 0.043	2.171AsnGln: 2.171 ± 0.041	1.945AsnArg: 1.945 ± 0.038	3.451AsnSer: 3.451 ± 0.105	2.989AsnThr: 2.989 ± 0.056	3.22AsnVal: 3.22 ± 0.067	0.625AsnTrp: 0.625 ± 0.022	1.702AsnTyr: 1.702 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.936ProAla: 2.936 ± 0.052	0.286ProCys: 0.286 ± 0.016	2.108ProAsp: 2.108 ± 0.042	2.954ProGlu: 2.954 ± 0.047	1.634ProPhe: 1.634 ± 0.037	2.166ProGly: 2.166 ± 0.046	0.761ProHis: 0.761 ± 0.021	2.574ProIle: 2.574 ± 0.054	2.152ProLys: 2.152 ± 0.046	3.733ProLeu: 3.733 ± 0.053	0.899ProMet: 0.899 ± 0.031	1.785ProAsn: 1.785 ± 0.036	0.917ProPro: 0.917 ± 0.028	1.301ProGln: 1.301 ± 0.032	1.17ProArg: 1.17 ± 0.032	2.324ProSer: 2.324 ± 0.052	2.12ProThr: 2.12 ± 0.047	2.689ProVal: 2.689 ± 0.048	0.476ProTrp: 0.476 ± 0.024	1.181ProTyr: 1.181 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.308GlnAla: 4.308 ± 0.066	0.398GlnCys: 0.398 ± 0.018	1.999GlnAsp: 1.999 ± 0.044	2.303GlnGlu: 2.303 ± 0.046	1.716GlnPhe: 1.716 ± 0.041	2.789GlnGly: 2.789 ± 0.05	1.247GlnHis: 1.247 ± 0.035	2.533GlnIle: 2.533 ± 0.042	2.246GlnLys: 2.246 ± 0.048	5.026GlnLeu: 5.026 ± 0.076	0.961GlnMet: 0.961 ± 0.025	1.833GlnAsn: 1.833 ± 0.037	1.558GlnPro: 1.558 ± 0.037	3.376GlnGln: 3.376 ± 0.078	1.96GlnArg: 1.96 ± 0.046	2.869GlnSer: 2.869 ± 0.053	2.173GlnThr: 2.173 ± 0.036	3.067GlnVal: 3.067 ± 0.051	0.712GlnTrp: 0.712 ± 0.023	1.434GlnTyr: 1.434 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.94ArgAla: 2.94 ± 0.05	0.371ArgCys: 0.371 ± 0.018	2.077ArgAsp: 2.077 ± 0.04	2.442ArgGlu: 2.442 ± 0.05	1.909ArgPhe: 1.909 ± 0.04	2.271ArgGly: 2.271 ± 0.05	0.873ArgHis: 0.873 ± 0.027	2.823ArgIle: 2.823 ± 0.047	2.283ArgLys: 2.283 ± 0.05	4.139ArgLeu: 4.139 ± 0.064	0.992ArgMet: 0.992 ± 0.027	1.85ArgAsn: 1.85 ± 0.041	1.298ArgPro: 1.298 ± 0.034	1.783ArgGln: 1.783 ± 0.042	1.812ArgArg: 1.812 ± 0.045	2.314ArgSer: 2.314 ± 0.045	1.88ArgThr: 1.88 ± 0.039	2.674ArgVal: 2.674 ± 0.043	0.523ArgTrp: 0.523 ± 0.021	1.517ArgTyr: 1.517 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.042SerAla: 6.042 ± 0.074	0.643SerCys: 0.643 ± 0.026	4.041SerAsp: 4.041 ± 0.091	3.967SerGlu: 3.967 ± 0.066	3.227SerPhe: 3.227 ± 0.054	4.818SerGly: 4.818 ± 0.06	1.496SerHis: 1.496 ± 0.035	4.719SerIle: 4.719 ± 0.076	3.648SerLys: 3.648 ± 0.055	7.022SerLeu: 7.022 ± 0.069	1.576SerMet: 1.576 ± 0.038	3.311SerAsn: 3.311 ± 0.073	2.475SerPro: 2.475 ± 0.04	3.028SerGln: 3.028 ± 0.055	2.631SerArg: 2.631 ± 0.047	4.873SerSer: 4.873 ± 0.083	3.918SerThr: 3.918 ± 0.07	4.643SerVal: 4.643 ± 0.058	0.776SerTrp: 0.776 ± 0.026	2.249SerTyr: 2.249 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.718ThrAla: 4.718 ± 0.102	0.481ThrCys: 0.481 ± 0.019	3.248ThrAsp: 3.248 ± 0.059	3.304ThrGlu: 3.304 ± 0.049	2.428ThrPhe: 2.428 ± 0.063	4.073ThrGly: 4.073 ± 0.069	1.122ThrHis: 1.122 ± 0.035	3.975ThrIle: 3.975 ± 0.067	2.808ThrLys: 2.808 ± 0.051	6.443ThrLeu: 6.443 ± 0.073	1.132ThrMet: 1.132 ± 0.03	2.541ThrAsn: 2.541 ± 0.061	2.611ThrPro: 2.611 ± 0.058	2.259ThrGln: 2.259 ± 0.052	1.956ThrArg: 1.956 ± 0.043	3.74ThrSer: 3.74 ± 0.062	3.185ThrThr: 3.185 ± 0.061	3.807ThrVal: 3.807 ± 0.061	0.652ThrTrp: 0.652 ± 0.024	1.525ThrTyr: 1.525 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	5.951ValAla: 5.951 ± 0.08	0.667ValCys: 0.667 ± 0.023	4.133ValAsp: 4.133 ± 0.065	4.172ValGlu: 4.172 ± 0.061	2.753ValPhe: 2.753 ± 0.047	4.155ValGly: 4.155 ± 0.066	1.199ValHis: 1.199 ± 0.033	5.122ValIle: 5.122 ± 0.067	3.831ValLys: 3.831 ± 0.067	6.348ValLeu: 6.348 ± 0.073	1.679ValMet: 1.679 ± 0.042	3.573ValAsn: 3.573 ± 0.063	2.34ValPro: 2.34 ± 0.043	2.101ValGln: 2.101 ± 0.042	2.377ValArg: 2.377 ± 0.049	4.694ValSer: 4.694 ± 0.072	4.075ValThr: 4.075 ± 0.093	4.72ValVal: 4.72 ± 0.071	0.641ValTrp: 0.641 ± 0.025	1.771ValTyr: 1.771 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.697TrpAla: 0.697 ± 0.026	0.128TrpCys: 0.128 ± 0.01	0.502TrpAsp: 0.502 ± 0.017	0.398TrpGlu: 0.398 ± 0.016	0.534TrpPhe: 0.534 ± 0.02	0.726TrpGly: 0.726 ± 0.026	0.377TrpHis: 0.377 ± 0.017	0.628TrpIle: 0.628 ± 0.023	0.44TrpLys: 0.44 ± 0.018	1.706TrpLeu: 1.706 ± 0.043	0.246TrpMet: 0.246 ± 0.014	0.433TrpAsn: 0.433 ± 0.02	0.435TrpPro: 0.435 ± 0.019	1.165TrpGln: 1.165 ± 0.034	0.612TrpArg: 0.612 ± 0.022	0.704TrpSer: 0.704 ± 0.025	0.469TrpThr: 0.469 ± 0.019	0.725TrpVal: 0.725 ± 0.024	0.159TrpTrp: 0.159 ± 0.01	0.359TrpTyr: 0.359 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.293TyrAla: 2.293 ± 0.046	0.408TyrCys: 0.408 ± 0.019	1.657TyrAsp: 1.657 ± 0.036	1.421TyrGlu: 1.421 ± 0.037	1.589TyrPhe: 1.589 ± 0.038	2.083TyrGly: 2.083 ± 0.043	0.773TyrHis: 0.773 ± 0.027	1.976TyrIle: 1.976 ± 0.037	1.583TyrLys: 1.583 ± 0.037	3.367TyrLeu: 3.367 ± 0.061	0.643TyrMet: 0.643 ± 0.023	1.41TyrAsn: 1.41 ± 0.036	1.255TyrPro: 1.255 ± 0.037	2.128TyrGln: 2.128 ± 0.041	1.485TyrArg: 1.485 ± 0.034	2.223TyrSer: 2.223 ± 0.039	1.473TyrThr: 1.473 ± 0.042	1.718TyrVal: 1.718 ± 0.033	0.44TyrTrp: 0.44 ± 0.017	1.133TyrTyr: 1.133 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3841 proteins (1307913 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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