Sulfolobales Virus YNP2
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N7FZ19|A0A0N7FZ19_9VIRU Uncharacterized protein OS=Sulfolobales Virus YNP2 OX=1732180 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.42 RR3 pKa = 11.84 LLVLLLVPLMVSLLVVGDD21 pKa = 4.39 AGAILDD27 pKa = 4.07 WSDD30 pKa = 2.99 GSAVVTIQPLTEE42 pKa = 4.08 TVIDD46 pKa = 4.61 GYY48 pKa = 10.59 IVNAFYY54 pKa = 10.88 NGSNQVVLLAEE65 pKa = 5.17 PYY67 pKa = 10.07 YY68 pKa = 10.45 QGQNLQQVTFSLYY81 pKa = 10.5 DD82 pKa = 3.41 YY83 pKa = 7.77 YY84 pKa = 11.03 THH86 pKa = 6.7 QFIGNYY92 pKa = 8.62 TIEE95 pKa = 4.41 DD96 pKa = 4.18 NYY98 pKa = 11.11 TVVTVPSNITVIYY111 pKa = 10.0 IYY113 pKa = 10.23 MEE115 pKa = 4.25 GQQFGPFYY123 pKa = 10.16 ITVNGGNFAPPLLDD137 pKa = 3.23 QMLMYY142 pKa = 10.29 IIPLSVVALFGLRR155 pKa = 11.84 AGLRR159 pKa = 11.84 NVGLGLLVASIFTSAEE175 pKa = 3.8 MVALGVSNPWLYY187 pKa = 10.85 AIPTLEE193 pKa = 3.83 IMFGIILLWHH203 pKa = 6.34 SVQTSGG209 pKa = 5.15
Molecular weight: 23.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.09
IPC2_protein 4.164
IPC_protein 4.05
Toseland 3.859
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.19
Wikipedia 3.948
Rodwell 3.884
Grimsley 3.783
Solomon 4.012
Lehninger 3.973
Nozaki 4.151
DTASelect 4.342
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.164
Patrickios 0.006
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.086
Protein with the highest isoelectric point:
>tr|A0A0N9NYU7|A0A0N9NYU7_9VIRU Uncharacterized protein OS=Sulfolobales Virus YNP2 OX=1732180 PE=4 SV=1
MM1 pKa = 6.73 AHH3 pKa = 7.51 KK4 pKa = 10.45 YY5 pKa = 7.27 HH6 pKa = 5.76 THH8 pKa = 6.01 SGRR11 pKa = 11.84 LVEE14 pKa = 4.04 WRR16 pKa = 11.84 RR17 pKa = 11.84 TFAEE21 pKa = 4.05 AAKK24 pKa = 9.31 MCSEE28 pKa = 4.2 KK29 pKa = 11.1 VKK31 pKa = 10.64 GLPKK35 pKa = 10.12 GQKK38 pKa = 7.92 LQAYY42 pKa = 7.47 RR43 pKa = 11.84 MCMRR47 pKa = 11.84 EE48 pKa = 4.01 TLRR51 pKa = 11.84 KK52 pKa = 9.35 KK53 pKa = 10.53
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.273
IPC2_protein 9.648
IPC_protein 9.853
Toseland 10.657
ProMoST 10.248
Dawson 10.745
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 11.257
Grimsley 10.774
Solomon 10.789
Lehninger 10.774
Nozaki 10.628
DTASelect 10.35
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 11.023
IPC_peptide 10.804
IPC2_peptide 9.18
IPC2.peptide.svr19 8.577
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
10418
45
1282
173.6
19.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.846 ± 0.362
0.49 ± 0.094
3.974 ± 0.386
6.431 ± 0.537
4.55 ± 0.377
5.51 ± 0.495
1.056 ± 0.115
8.409 ± 0.371
6.575 ± 0.749
10.299 ± 0.502
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.352 ± 0.287
5.356 ± 0.442
5.212 ± 0.581
3.686 ± 0.73
4.703 ± 0.385
6.038 ± 0.346
5.414 ± 0.356
7.717 ± 0.45
1.209 ± 0.124
5.155 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here