Pseudoalteromonas phage PHS21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1M562|A0A2S1M562_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PHS21 OX=1955235 PE=4 SV=1
MM1 pKa = 7.43SANNALQAALISALRR16 pKa = 11.84TDD18 pKa = 4.09ADD20 pKa = 5.05LIALLPTYY28 pKa = 10.34AAVPAVFYY36 pKa = 8.54YY37 pKa = 10.64VPQGFDD43 pKa = 3.3DD44 pKa = 3.88SQSYY48 pKa = 6.76VTIYY52 pKa = 9.6EE53 pKa = 3.93ISMLPDD59 pKa = 3.22DD60 pKa = 4.7TVNTDD65 pKa = 2.71GFAAEE70 pKa = 4.36VTIHH74 pKa = 5.41TWVEE78 pKa = 4.09EE79 pKa = 4.03EE80 pKa = 4.15TTAQTGAIMQSIYY93 pKa = 11.37NLLHH97 pKa = 6.93KK98 pKa = 10.68NDD100 pKa = 4.11TLPLTGYY107 pKa = 9.94SVTGIDD113 pKa = 5.51CEE115 pKa = 4.22FQTILRR121 pKa = 11.84DD122 pKa = 3.5PDD124 pKa = 3.01GKK126 pKa = 8.88TRR128 pKa = 11.84HH129 pKa = 5.16GVQRR133 pKa = 11.84FNVSFEE139 pKa = 3.93PAVIYY144 pKa = 8.04PPCNN148 pKa = 3.2

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223SED7|A0A223SED7_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PHS21 OX=1955235 PE=4 SV=1
MM1 pKa = 7.53NSLSDD6 pKa = 3.55MTDD9 pKa = 2.9LSFTQTKK16 pKa = 9.99RR17 pKa = 11.84AMTSTHH23 pKa = 6.87LSRR26 pKa = 11.84VSHH29 pKa = 6.32QYY31 pKa = 10.67RR32 pKa = 11.84PMWAIAATNNRR43 pKa = 11.84KK44 pKa = 9.25PKK46 pKa = 10.25VLIDD50 pKa = 3.48RR51 pKa = 11.84VRR53 pKa = 11.84DD54 pKa = 3.89GKK56 pKa = 10.8RR57 pKa = 11.84SNPTVFMGCC66 pKa = 3.58

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10166

39

831

203.3

22.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.332 ± 0.603

1.072 ± 0.135

6.522 ± 0.321

6.61 ± 0.263

4.004 ± 0.201

7.043 ± 0.418

1.554 ± 0.18

6.177 ± 0.244

7.387 ± 0.457

8.283 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.252

5.489 ± 0.265

3.049 ± 0.227

3.659 ± 0.255

3.846 ± 0.197

7.073 ± 0.311

5.961 ± 0.287

6.355 ± 0.292

1.387 ± 0.134

3.492 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski