Amino acid dipepetide frequency for Pseudoalteromonas phage PHS21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.46AlaAla: 8.46 ± 1.957
0.492AlaCys: 0.492 ± 0.233
4.525AlaAsp: 4.525 ± 0.967
5.804AlaGlu: 5.804 ± 0.878
3.345AlaPhe: 3.345 ± 0.568
6.394AlaGly: 6.394 ± 0.831
1.377AlaHis: 1.377 ± 0.374
5.903AlaIle: 5.903 ± 0.829
6.886AlaLys: 6.886 ± 1.156
7.083AlaLeu: 7.083 ± 0.78
2.951AlaMet: 2.951 ± 0.862
4.82AlaAsn: 4.82 ± 0.705
2.361AlaPro: 2.361 ± 0.384
2.361AlaGln: 2.361 ± 0.412
2.361AlaArg: 2.361 ± 0.434
4.82AlaSer: 4.82 ± 0.552
3.64AlaThr: 3.64 ± 0.648
6.296AlaVal: 6.296 ± 0.775
0.59AlaTrp: 0.59 ± 0.202
2.853AlaTyr: 2.853 ± 0.461
0.0AlaXaa: 0.0 ± 0.0
Cys
0.295CysAla: 0.295 ± 0.148
0.394CysCys: 0.394 ± 0.182
0.689CysAsp: 0.689 ± 0.258
1.181CysGlu: 1.181 ± 0.362
0.492CysPhe: 0.492 ± 0.199
0.59CysGly: 0.59 ± 0.237
0.197CysHis: 0.197 ± 0.115
0.394CysIle: 0.394 ± 0.188
0.59CysLys: 0.59 ± 0.262
1.181CysLeu: 1.181 ± 0.267
0.394CysMet: 0.394 ± 0.188
0.59CysAsn: 0.59 ± 0.257
0.295CysPro: 0.295 ± 0.206
0.59CysGln: 0.59 ± 0.224
0.295CysArg: 0.295 ± 0.163
0.394CysSer: 0.394 ± 0.19
0.689CysThr: 0.689 ± 0.201
0.885CysVal: 0.885 ± 0.263
0.295CysTrp: 0.295 ± 0.167
0.295CysTyr: 0.295 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
5.312AspAla: 5.312 ± 0.558
0.689AspCys: 0.689 ± 0.235
4.624AspAsp: 4.624 ± 0.806
5.312AspGlu: 5.312 ± 0.865
3.148AspPhe: 3.148 ± 0.63
5.214AspGly: 5.214 ± 0.769
0.59AspHis: 0.59 ± 0.222
4.624AspIle: 4.624 ± 0.954
4.23AspLys: 4.23 ± 0.65
6.099AspLeu: 6.099 ± 1.283
1.574AspMet: 1.574 ± 0.412
2.558AspAsn: 2.558 ± 0.466
1.476AspPro: 1.476 ± 0.405
1.574AspGln: 1.574 ± 0.357
1.771AspArg: 1.771 ± 0.416
4.722AspSer: 4.722 ± 0.822
3.935AspThr: 3.935 ± 0.587
3.542AspVal: 3.542 ± 0.498
1.181AspTrp: 1.181 ± 0.363
3.05AspTyr: 3.05 ± 0.398
0.0AspXaa: 0.0 ± 0.0
Glu
4.722GluAla: 4.722 ± 0.859
0.787GluCys: 0.787 ± 0.305
2.755GluAsp: 2.755 ± 0.516
2.951GluGlu: 2.951 ± 0.502
3.148GluPhe: 3.148 ± 0.561
3.443GluGly: 3.443 ± 0.596
1.574GluHis: 1.574 ± 0.402
5.312GluIle: 5.312 ± 0.701
5.017GluLys: 5.017 ± 0.793
8.756GluLeu: 8.756 ± 1.106
2.263GluMet: 2.263 ± 0.601
3.443GluAsn: 3.443 ± 0.592
0.984GluPro: 0.984 ± 0.328
2.558GluGln: 2.558 ± 0.632
1.968GluArg: 1.968 ± 0.363
4.919GluSer: 4.919 ± 0.683
3.246GluThr: 3.246 ± 0.531
4.722GluVal: 4.722 ± 0.698
1.082GluTrp: 1.082 ± 0.278
2.459GluTyr: 2.459 ± 0.465
0.0GluXaa: 0.0 ± 0.0
Phe
3.345PheAla: 3.345 ± 0.524
0.59PheCys: 0.59 ± 0.211
2.951PheAsp: 2.951 ± 0.546
2.853PheGlu: 2.853 ± 0.507
1.279PhePhe: 1.279 ± 0.43
3.443PheGly: 3.443 ± 0.517
0.59PheHis: 0.59 ± 0.296
2.066PheIle: 2.066 ± 0.365
4.23PheLys: 4.23 ± 0.839
2.361PheLeu: 2.361 ± 0.467
1.377PheMet: 1.377 ± 0.387
2.263PheAsn: 2.263 ± 0.431
1.181PhePro: 1.181 ± 0.373
0.885PheGln: 0.885 ± 0.237
1.279PheArg: 1.279 ± 0.322
3.148PheSer: 3.148 ± 0.638
2.755PheThr: 2.755 ± 0.523
1.181PheVal: 1.181 ± 0.287
0.689PheTrp: 0.689 ± 0.24
1.574PheTyr: 1.574 ± 0.341
0.0PheXaa: 0.0 ± 0.0
Gly
7.083GlyAla: 7.083 ± 0.799
0.492GlyCys: 0.492 ± 0.216
4.919GlyAsp: 4.919 ± 0.771
4.919GlyGlu: 4.919 ± 0.554
2.558GlyPhe: 2.558 ± 0.527
6.493GlyGly: 6.493 ± 1.668
0.59GlyHis: 0.59 ± 0.186
3.345GlyIle: 3.345 ± 0.761
5.214GlyLys: 5.214 ± 0.7
5.706GlyLeu: 5.706 ± 0.702
0.984GlyMet: 0.984 ± 0.298
3.64GlyAsn: 3.64 ± 0.559
0.394GlyPro: 0.394 ± 0.161
2.951GlyGln: 2.951 ± 0.481
2.066GlyArg: 2.066 ± 0.425
4.82GlySer: 4.82 ± 0.785
4.624GlyThr: 4.624 ± 0.911
6.296GlyVal: 6.296 ± 0.904
0.984GlyTrp: 0.984 ± 0.269
2.361GlyTyr: 2.361 ± 0.566
0.0GlyXaa: 0.0 ± 0.0
His
1.082HisAla: 1.082 ± 0.296
0.295HisCys: 0.295 ± 0.223
0.984HisAsp: 0.984 ± 0.386
1.082HisGlu: 1.082 ± 0.313
0.787HisPhe: 0.787 ± 0.329
0.984HisGly: 0.984 ± 0.273
0.098HisHis: 0.098 ± 0.074
0.787HisIle: 0.787 ± 0.256
1.279HisLys: 1.279 ± 0.344
1.672HisLeu: 1.672 ± 0.39
0.394HisMet: 0.394 ± 0.177
1.082HisAsn: 1.082 ± 0.327
0.394HisPro: 0.394 ± 0.179
0.689HisGln: 0.689 ± 0.274
0.787HisArg: 0.787 ± 0.368
0.689HisSer: 0.689 ± 0.278
0.984HisThr: 0.984 ± 0.271
0.394HisVal: 0.394 ± 0.201
0.098HisTrp: 0.098 ± 0.101
0.984HisTyr: 0.984 ± 0.317
0.0HisXaa: 0.0 ± 0.0
Ile
4.23IleAla: 4.23 ± 0.573
0.59IleCys: 0.59 ± 0.204
5.116IleAsp: 5.116 ± 0.487
4.919IleGlu: 4.919 ± 0.704
1.181IlePhe: 1.181 ± 0.338
3.738IleGly: 3.738 ± 0.51
0.885IleHis: 0.885 ± 0.252
3.935IleIle: 3.935 ± 0.794
4.329IleLys: 4.329 ± 0.677
3.64IleLeu: 3.64 ± 0.636
1.574IleMet: 1.574 ± 0.347
4.82IleAsn: 4.82 ± 0.846
1.476IlePro: 1.476 ± 0.429
2.066IleGln: 2.066 ± 0.382
3.542IleArg: 3.542 ± 0.872
4.919IleSer: 4.919 ± 0.778
4.132IleThr: 4.132 ± 0.605
3.64IleVal: 3.64 ± 0.726
0.689IleTrp: 0.689 ± 0.286
2.263IleTyr: 2.263 ± 0.41
0.0IleXaa: 0.0 ± 0.0
Lys
8.362LysAla: 8.362 ± 0.982
1.181LysCys: 1.181 ± 0.328
4.132LysAsp: 4.132 ± 0.679
3.738LysGlu: 3.738 ± 0.612
2.066LysPhe: 2.066 ± 0.384
4.23LysGly: 4.23 ± 0.803
1.869LysHis: 1.869 ± 0.423
4.722LysIle: 4.722 ± 0.709
5.017LysLys: 5.017 ± 0.929
6.001LysLeu: 6.001 ± 1.115
3.148LysMet: 3.148 ± 0.683
2.755LysAsn: 2.755 ± 0.405
2.656LysPro: 2.656 ± 0.494
3.05LysGln: 3.05 ± 0.599
2.951LysArg: 2.951 ± 0.717
5.903LysSer: 5.903 ± 0.888
4.427LysThr: 4.427 ± 0.56
3.738LysVal: 3.738 ± 0.575
1.672LysTrp: 1.672 ± 0.415
2.263LysTyr: 2.263 ± 0.481
0.0LysXaa: 0.0 ± 0.0
Leu
5.607LeuAla: 5.607 ± 0.842
0.59LeuCys: 0.59 ± 0.24
5.116LeuAsp: 5.116 ± 0.765
5.214LeuGlu: 5.214 ± 0.797
3.443LeuPhe: 3.443 ± 0.621
5.411LeuGly: 5.411 ± 0.733
1.771LeuHis: 1.771 ± 0.451
5.804LeuIle: 5.804 ± 0.594
7.182LeuLys: 7.182 ± 0.803
6.69LeuLeu: 6.69 ± 0.833
2.755LeuMet: 2.755 ± 0.535
3.935LeuAsn: 3.935 ± 0.582
3.542LeuPro: 3.542 ± 0.629
3.443LeuGln: 3.443 ± 0.576
2.951LeuArg: 2.951 ± 0.622
5.903LeuSer: 5.903 ± 0.881
5.017LeuThr: 5.017 ± 0.728
4.624LeuVal: 4.624 ± 0.837
0.689LeuTrp: 0.689 ± 0.241
3.148LeuTyr: 3.148 ± 0.534
0.0LeuXaa: 0.0 ± 0.0
Met
2.361MetAla: 2.361 ± 0.523
0.098MetCys: 0.098 ± 0.092
0.885MetAsp: 0.885 ± 0.283
1.377MetGlu: 1.377 ± 0.412
0.885MetPhe: 0.885 ± 0.396
1.869MetGly: 1.869 ± 0.392
0.098MetHis: 0.098 ± 0.093
2.459MetIle: 2.459 ± 0.413
2.263MetLys: 2.263 ± 0.458
2.459MetLeu: 2.459 ± 0.511
0.197MetMet: 0.197 ± 0.128
2.263MetAsn: 2.263 ± 0.404
1.082MetPro: 1.082 ± 0.301
1.672MetGln: 1.672 ± 0.461
1.279MetArg: 1.279 ± 0.35
2.164MetSer: 2.164 ± 0.54
1.771MetThr: 1.771 ± 0.482
0.984MetVal: 0.984 ± 0.287
0.394MetTrp: 0.394 ± 0.181
0.492MetTyr: 0.492 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
4.033AsnAla: 4.033 ± 0.7
0.59AsnCys: 0.59 ± 0.31
3.837AsnAsp: 3.837 ± 0.559
3.738AsnGlu: 3.738 ± 0.593
2.459AsnPhe: 2.459 ± 0.35
5.116AsnGly: 5.116 ± 0.732
0.59AsnHis: 0.59 ± 0.283
3.443AsnIle: 3.443 ± 0.616
4.329AsnLys: 4.329 ± 0.583
4.132AsnLeu: 4.132 ± 0.633
0.984AsnMet: 0.984 ± 0.294
3.246AsnAsn: 3.246 ± 0.667
2.558AsnPro: 2.558 ± 0.5
1.181AsnGln: 1.181 ± 0.341
2.459AsnArg: 2.459 ± 0.475
3.837AsnSer: 3.837 ± 0.534
2.951AsnThr: 2.951 ± 0.438
2.951AsnVal: 2.951 ± 0.625
1.082AsnTrp: 1.082 ± 0.378
1.377AsnTyr: 1.377 ± 0.381
0.0AsnXaa: 0.0 ± 0.0
Pro
2.558ProAla: 2.558 ± 0.48
0.197ProCys: 0.197 ± 0.128
1.968ProAsp: 1.968 ± 0.391
2.361ProGlu: 2.361 ± 0.436
1.279ProPhe: 1.279 ± 0.522
0.689ProGly: 0.689 ± 0.223
0.394ProHis: 0.394 ± 0.17
0.885ProIle: 0.885 ± 0.246
2.066ProLys: 2.066 ± 0.522
3.05ProLeu: 3.05 ± 0.369
0.984ProMet: 0.984 ± 0.344
2.361ProAsn: 2.361 ± 0.558
0.885ProPro: 0.885 ± 0.295
0.689ProGln: 0.689 ± 0.315
1.672ProArg: 1.672 ± 0.441
1.869ProSer: 1.869 ± 0.326
2.656ProThr: 2.656 ± 0.501
2.361ProVal: 2.361 ± 0.461
0.59ProTrp: 0.59 ± 0.222
0.984ProTyr: 0.984 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
4.033GlnAla: 4.033 ± 0.755
0.394GlnCys: 0.394 ± 0.19
1.869GlnAsp: 1.869 ± 0.526
2.164GlnGlu: 2.164 ± 0.561
1.672GlnPhe: 1.672 ± 0.459
1.869GlnGly: 1.869 ± 0.368
0.984GlnHis: 0.984 ± 0.314
2.066GlnIle: 2.066 ± 0.438
1.476GlnLys: 1.476 ± 0.353
3.542GlnLeu: 3.542 ± 0.708
0.984GlnMet: 0.984 ± 0.332
1.869GlnAsn: 1.869 ± 0.47
0.787GlnPro: 0.787 ± 0.228
1.476GlnGln: 1.476 ± 0.452
1.082GlnArg: 1.082 ± 0.289
3.443GlnSer: 3.443 ± 0.629
2.263GlnThr: 2.263 ± 0.423
2.459GlnVal: 2.459 ± 0.356
0.59GlnTrp: 0.59 ± 0.362
1.574GlnTyr: 1.574 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
3.246ArgAla: 3.246 ± 0.524
0.394ArgCys: 0.394 ± 0.167
2.459ArgAsp: 2.459 ± 0.406
2.164ArgGlu: 2.164 ± 0.36
1.968ArgPhe: 1.968 ± 0.491
2.361ArgGly: 2.361 ± 0.487
0.984ArgHis: 0.984 ± 0.344
2.164ArgIle: 2.164 ± 0.45
2.853ArgLys: 2.853 ± 0.487
3.64ArgLeu: 3.64 ± 0.691
0.59ArgMet: 0.59 ± 0.244
1.574ArgAsn: 1.574 ± 0.404
1.279ArgPro: 1.279 ± 0.619
1.968ArgGln: 1.968 ± 0.425
1.279ArgArg: 1.279 ± 0.433
2.558ArgSer: 2.558 ± 0.471
1.869ArgThr: 1.869 ± 0.338
2.853ArgVal: 2.853 ± 0.464
0.787ArgTrp: 0.787 ± 0.235
1.476ArgTyr: 1.476 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
6.985SerAla: 6.985 ± 1.028
0.394SerCys: 0.394 ± 0.221
6.886SerAsp: 6.886 ± 0.759
4.033SerGlu: 4.033 ± 0.733
2.951SerPhe: 2.951 ± 0.466
5.312SerGly: 5.312 ± 0.934
1.082SerHis: 1.082 ± 0.26
3.738SerIle: 3.738 ± 0.787
5.509SerLys: 5.509 ± 0.687
5.607SerLeu: 5.607 ± 0.692
1.574SerMet: 1.574 ± 0.347
4.132SerAsn: 4.132 ± 0.482
2.755SerPro: 2.755 ± 0.546
2.853SerGln: 2.853 ± 0.394
2.951SerArg: 2.951 ± 0.386
4.919SerSer: 4.919 ± 0.969
2.066SerThr: 2.066 ± 0.446
4.132SerVal: 4.132 ± 0.493
0.59SerTrp: 0.59 ± 0.237
2.263SerTyr: 2.263 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
4.132ThrAla: 4.132 ± 0.935
0.394ThrCys: 0.394 ± 0.188
3.837ThrAsp: 3.837 ± 0.762
4.329ThrGlu: 4.329 ± 0.626
3.05ThrPhe: 3.05 ± 0.552
5.312ThrGly: 5.312 ± 0.777
0.394ThrHis: 0.394 ± 0.202
3.935ThrIle: 3.935 ± 0.686
3.542ThrLys: 3.542 ± 0.645
2.853ThrLeu: 2.853 ± 0.544
1.181ThrMet: 1.181 ± 0.428
3.443ThrAsn: 3.443 ± 0.72
2.558ThrPro: 2.558 ± 0.59
2.656ThrGln: 2.656 ± 0.612
2.656ThrArg: 2.656 ± 0.584
3.443ThrSer: 3.443 ± 0.597
3.542ThrThr: 3.542 ± 0.63
5.116ThrVal: 5.116 ± 0.794
0.689ThrTrp: 0.689 ± 0.234
2.558ThrTyr: 2.558 ± 0.59
0.0ThrXaa: 0.0 ± 0.0
Val
4.525ValAla: 4.525 ± 0.61
1.377ValCys: 1.377 ± 0.355
4.722ValAsp: 4.722 ± 0.683
5.017ValGlu: 5.017 ± 0.72
2.263ValPhe: 2.263 ± 0.447
3.935ValGly: 3.935 ± 0.791
0.787ValHis: 0.787 ± 0.305
3.542ValIle: 3.542 ± 0.426
4.624ValLys: 4.624 ± 0.51
4.23ValLeu: 4.23 ± 0.565
2.066ValMet: 2.066 ± 0.51
3.443ValAsn: 3.443 ± 0.617
2.263ValPro: 2.263 ± 0.747
1.968ValGln: 1.968 ± 0.416
2.755ValArg: 2.755 ± 0.397
4.132ValSer: 4.132 ± 0.585
5.509ValThr: 5.509 ± 1.075
4.033ValVal: 4.033 ± 0.607
0.689ValTrp: 0.689 ± 0.287
1.672ValTyr: 1.672 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.492TrpAla: 0.492 ± 0.227
0.197TrpCys: 0.197 ± 0.115
0.787TrpAsp: 0.787 ± 0.228
0.787TrpGlu: 0.787 ± 0.356
0.984TrpPhe: 0.984 ± 0.341
1.082TrpGly: 1.082 ± 0.337
0.394TrpHis: 0.394 ± 0.192
0.689TrpIle: 0.689 ± 0.233
0.492TrpLys: 0.492 ± 0.256
1.377TrpLeu: 1.377 ± 0.337
0.295TrpMet: 0.295 ± 0.171
0.689TrpAsn: 0.689 ± 0.243
0.689TrpPro: 0.689 ± 0.24
0.295TrpGln: 0.295 ± 0.237
1.279TrpArg: 1.279 ± 0.332
0.787TrpSer: 0.787 ± 0.261
0.787TrpThr: 0.787 ± 0.308
1.279TrpVal: 1.279 ± 0.329
0.197TrpTrp: 0.197 ± 0.151
0.295TrpTyr: 0.295 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.37
0.59TyrCys: 0.59 ± 0.27
2.164TyrAsp: 2.164 ± 0.529
2.164TyrGlu: 2.164 ± 0.506
1.082TyrPhe: 1.082 ± 0.318
2.951TyrGly: 2.951 ± 0.39
0.295TyrHis: 0.295 ± 0.155
1.968TyrIle: 1.968 ± 0.451
2.755TyrLys: 2.755 ± 0.596
2.755TyrLeu: 2.755 ± 0.511
0.689TyrMet: 0.689 ± 0.276
1.968TyrAsn: 1.968 ± 0.355
0.885TyrPro: 0.885 ± 0.319
1.672TyrGln: 1.672 ± 0.509
1.082TyrArg: 1.082 ± 0.309
3.345TyrSer: 3.345 ± 0.528
2.755TyrThr: 2.755 ± 0.524
2.066TyrVal: 2.066 ± 0.412
0.295TyrTrp: 0.295 ± 0.135
0.984TyrTyr: 0.984 ± 0.291
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10166 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski