Vibrio sp. HA2012

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8H2L5|A0A2M8H2L5_9VIBR 50S ribosomal protein L35 OS=Vibrio sp. HA2012 OX=1971595 GN=rpmI PE=3 SV=1
MM1 pKa = 7.89KK2 pKa = 10.62YY3 pKa = 10.62LILSILAALLAIPAIAEE20 pKa = 4.15DD21 pKa = 4.03SQTASGQGADD31 pKa = 3.44FSMAIDD37 pKa = 4.38CGDD40 pKa = 3.67FSSGEE45 pKa = 4.22TFNPEE50 pKa = 4.1DD51 pKa = 3.6PAMDD55 pKa = 3.49QVTLSTEE62 pKa = 3.59ITHH65 pKa = 6.68QIISLCPGVAEE76 pKa = 5.08VISSTDD82 pKa = 2.91SQTAQLVGATFKK94 pKa = 10.52DD95 pKa = 3.49TAEE98 pKa = 5.32DD99 pKa = 3.25IAADD103 pKa = 3.61IARR106 pKa = 11.84KK107 pKa = 9.56VAVQYY112 pKa = 11.43DD113 pKa = 3.91FTTEE117 pKa = 3.82FDD119 pKa = 3.88DD120 pKa = 5.76RR121 pKa = 11.84SCQVLRR127 pKa = 11.84INPGSQSKK135 pKa = 10.19IMCDD139 pKa = 3.38TNEE142 pKa = 4.5SNPSAYY148 pKa = 9.95CAEE151 pKa = 4.8LSQEE155 pKa = 4.31TISEE159 pKa = 3.91IMALCDD165 pKa = 3.71SLTSNN170 pKa = 4.69

Molecular weight: 18.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8GV83|A0A2M8GV83_9VIBR Pilus assembly protein PilN OS=Vibrio sp. HA2012 OX=1971595 GN=CSW98_15525 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3382

0

3382

1105604

29

2826

326.9

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.454 ± 0.045

1.101 ± 0.015

5.521 ± 0.035

6.549 ± 0.04

3.994 ± 0.026

6.938 ± 0.042

2.235 ± 0.018

6.717 ± 0.039

5.216 ± 0.037

10.357 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.021

4.078 ± 0.028

3.864 ± 0.028

4.393 ± 0.036

4.817 ± 0.031

6.498 ± 0.037

5.377 ± 0.03

6.896 ± 0.036

1.151 ± 0.016

3.084 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski