Trichoplusia ni TED virus
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q65353|Q65353_9VIRU ORF B OS=Trichoplusia ni TED virus OX=2083181 PE=4 SV=1
MM1 pKa = 7.47 PHH3 pKa = 7.06 DD4 pKa = 4.68 PDD6 pKa = 4.61 VIFKK10 pKa = 10.68 ALRR13 pKa = 11.84 LVPEE17 pKa = 4.49 FNGNPNILTRR27 pKa = 11.84 FINICDD33 pKa = 3.8 KK34 pKa = 11.05 LVEE37 pKa = 4.34 QYY39 pKa = 11.57 ASAEE43 pKa = 4.13 PGSEE47 pKa = 4.21 LGNLCLLNGILNKK60 pKa = 9.29 VTGTAASTINANGIPEE76 pKa = 4.21 TWVGIRR82 pKa = 11.84 SSLINNFSDD91 pKa = 4.42 QRR93 pKa = 11.84 DD94 pKa = 3.53 EE95 pKa = 4.04 TALYY99 pKa = 10.68 NDD101 pKa = 5.19 LSLASQGNKK110 pKa = 8.47 TPQEE114 pKa = 4.35 FYY116 pKa = 10.41 EE117 pKa = 4.16 QCQTLFSTIMTYY129 pKa = 8.58 VTLHH133 pKa = 5.63 EE134 pKa = 4.46 TLPTTIEE141 pKa = 4.06 AKK143 pKa = 9.59 RR144 pKa = 11.84 ALYY147 pKa = 10.42 KK148 pKa = 10.51 KK149 pKa = 9.0 VTVQAFVRR157 pKa = 11.84 GLKK160 pKa = 10.12 EE161 pKa = 3.74 PLGSRR166 pKa = 11.84 IRR168 pKa = 11.84 CMRR171 pKa = 11.84 PEE173 pKa = 4.37 TIEE176 pKa = 3.86 KK177 pKa = 10.03 ALEE180 pKa = 3.99 YY181 pKa = 10.46 VQEE184 pKa = 4.2 EE185 pKa = 4.66 LNVIYY190 pKa = 9.95 LQQRR194 pKa = 11.84 NEE196 pKa = 3.74 SSRR199 pKa = 11.84 AHH201 pKa = 6.7 SSPKK205 pKa = 9.52 MLPIPQQSPVTPFNTLGIHH224 pKa = 6.76 RR225 pKa = 11.84 PPVPNWPVPMGQRR238 pKa = 11.84 GNQPPPQPFKK248 pKa = 11.34 FNVPNQYY255 pKa = 10.36 HH256 pKa = 5.86 NRR258 pKa = 11.84 MPTKK262 pKa = 8.12 TQQMLRR268 pKa = 11.84 APPPNYY274 pKa = 9.69 HH275 pKa = 6.19 PQSNVFRR282 pKa = 11.84 LPPRR286 pKa = 11.84 NPPPNQIVKK295 pKa = 9.97 PMSGVQHH302 pKa = 6.26 FVPKK306 pKa = 9.31 TLPVMTGHH314 pKa = 7.7 DD315 pKa = 3.51 WRR317 pKa = 11.84 KK318 pKa = 10.37 SGNPPPNNYY327 pKa = 9.75 FKK329 pKa = 10.65 TRR331 pKa = 11.84 EE332 pKa = 3.98 LNVNEE337 pKa = 5.34 FYY339 pKa = 11.26 SSDD342 pKa = 3.44 DD343 pKa = 3.54 SYY345 pKa = 12.24 NSVDD349 pKa = 3.92 YY350 pKa = 11.01 YY351 pKa = 11.25 SEE353 pKa = 4.71 PGCDD357 pKa = 3.54 YY358 pKa = 10.76 YY359 pKa = 11.18 TDD361 pKa = 4.42 YY362 pKa = 11.73 YY363 pKa = 10.46 NNPYY367 pKa = 10.73 DD368 pKa = 4.03 YY369 pKa = 9.21 DD370 pKa = 4.21 TNATCYY376 pKa = 10.24 DD377 pKa = 3.86 LPYY380 pKa = 10.47 DD381 pKa = 3.9 ASEE384 pKa = 4.61 TEE386 pKa = 4.15 AQPGPSHH393 pKa = 5.49 VHH395 pKa = 6.26 EE396 pKa = 4.72 SQDD399 pKa = 3.62 FQSTKK404 pKa = 10.22 PSNEE408 pKa = 3.72 QGG410 pKa = 3.25
Molecular weight: 46.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.68
IPC2_protein 5.766
IPC_protein 5.817
Toseland 6.198
ProMoST 6.16
Dawson 6.084
Bjellqvist 6.084
Wikipedia 6.084
Rodwell 6.071
Grimsley 6.351
Solomon 6.084
Lehninger 6.071
Nozaki 6.351
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.453
Patrickios 3.63
IPC_peptide 6.097
IPC2_peptide 6.427
IPC2.peptide.svr19 6.379
Protein with the highest isoelectric point:
>tr|Q65354|Q65354_9VIRU ORF C OS=Trichoplusia ni TED virus OX=2083181 PE=4 SV=1
MM1 pKa = 7.38 IPARR5 pKa = 11.84 SSKK8 pKa = 10.3 LVRR11 pKa = 11.84 IPINATDD18 pKa = 4.05 GEE20 pKa = 4.73 VLVEE24 pKa = 3.92 EE25 pKa = 5.29 QMFCNCIVHH34 pKa = 6.23 EE35 pKa = 4.53 CVTMVKK41 pKa = 10.25 DD42 pKa = 3.29 GRR44 pKa = 11.84 GYY46 pKa = 11.08 VEE48 pKa = 5.54 LEE50 pKa = 3.89 NPTPNDD56 pKa = 3.1 VIFYY60 pKa = 10.13 LDD62 pKa = 3.39 QPASAEE68 pKa = 4.03 LFNIKK73 pKa = 8.53 CTQVEE78 pKa = 3.93 QSQRR82 pKa = 11.84 VDD84 pKa = 3.31 DD85 pKa = 3.94 VLSRR89 pKa = 11.84 LRR91 pKa = 11.84 TDD93 pKa = 3.51 HH94 pKa = 7.12 LNEE97 pKa = 3.92 EE98 pKa = 4.41 EE99 pKa = 4.27 KK100 pKa = 11.36 ANLLRR105 pKa = 11.84 LCSRR109 pKa = 11.84 YY110 pKa = 10.27 SDD112 pKa = 3.41 VFYY115 pKa = 10.69 IDD117 pKa = 4.92 GEE119 pKa = 4.35 ALTFTNKK126 pKa = 9.08 IKK128 pKa = 10.64 HH129 pKa = 6.57 RR130 pKa = 11.84 IRR132 pKa = 11.84 TTDD135 pKa = 3.38 EE136 pKa = 3.92 VPVYY140 pKa = 8.82 TKK142 pKa = 10.26 SYY144 pKa = 9.48 RR145 pKa = 11.84 YY146 pKa = 9.55 PFIHH150 pKa = 6.07 RR151 pKa = 11.84 QEE153 pKa = 4.06 VRR155 pKa = 11.84 DD156 pKa = 4.2 QITKK160 pKa = 9.53 MLDD163 pKa = 2.92 QGIIRR168 pKa = 11.84 PSDD171 pKa = 3.79 SAWSSPIWVVPKK183 pKa = 10.67 KK184 pKa = 10.04 IDD186 pKa = 3.79 ASGKK190 pKa = 7.82 QKK192 pKa = 9.69 WRR194 pKa = 11.84 LVVDD198 pKa = 4.37 FRR200 pKa = 11.84 KK201 pKa = 10.49 LNEE204 pKa = 3.68 KK205 pKa = 9.8 TIDD208 pKa = 3.73 DD209 pKa = 4.9 KK210 pKa = 11.96 YY211 pKa = 10.26 PIPNISDD218 pKa = 3.49 VLDD221 pKa = 3.72 KK222 pKa = 11.16 LGKK225 pKa = 9.22 CQYY228 pKa = 8.23 FTTLDD233 pKa = 3.85 LASGFYY239 pKa = 9.36 QVEE242 pKa = 4.36 MDD244 pKa = 4.09 PQDD247 pKa = 3.14 ISKK250 pKa = 8.82 TAFNVEE256 pKa = 3.98 HH257 pKa = 6.24 GHH259 pKa = 6.73 FEE261 pKa = 4.25 FLRR264 pKa = 11.84 MPMGLKK270 pKa = 10.23 NSPSTFQRR278 pKa = 11.84 VMDD281 pKa = 3.83 NVLRR285 pKa = 11.84 GLQNNICLVYY295 pKa = 10.53 LDD297 pKa = 5.49 DD298 pKa = 5.52 IIVYY302 pKa = 7.98 STSLQEE308 pKa = 3.93 HH309 pKa = 6.83 LEE311 pKa = 3.8 NLEE314 pKa = 4.22 RR315 pKa = 11.84 VFQRR319 pKa = 11.84 LRR321 pKa = 11.84 EE322 pKa = 4.32 SNFKK326 pKa = 10.37 IQMDD330 pKa = 3.23 KK331 pKa = 11.47 SEE333 pKa = 4.08 FLKK336 pKa = 11.32 LEE338 pKa = 3.97 TAYY341 pKa = 10.56 LGHH344 pKa = 7.09 IISRR348 pKa = 11.84 DD349 pKa = 3.71 GIKK352 pKa = 10.47 PNPDD356 pKa = 3.82 KK357 pKa = 10.97 ISAIQKK363 pKa = 8.88 YY364 pKa = 9.82 LIPKK368 pKa = 7.35 TPKK371 pKa = 9.95 EE372 pKa = 3.84 IKK374 pKa = 10.23 QFLGLLGYY382 pKa = 8.7 YY383 pKa = 10.12 RR384 pKa = 11.84 KK385 pKa = 10.1 FIPDD389 pKa = 4.22 FARR392 pKa = 11.84 LTKK395 pKa = 10.38 PLTQCLKK402 pKa = 10.36 KK403 pKa = 10.5 GSKK406 pKa = 8.61 VTLSPEE412 pKa = 3.78 YY413 pKa = 10.82 VNAFEE418 pKa = 4.5 HH419 pKa = 6.85 CKK421 pKa = 9.5 TLLTNDD427 pKa = 5.68 PILQYY432 pKa = 11.01 PDD434 pKa = 3.31 FTRR437 pKa = 11.84 EE438 pKa = 3.84 FNLTTDD444 pKa = 3.7 ASNFAIGAVLSQGPIGSDD462 pKa = 3.06 KK463 pKa = 10.15 PVCYY467 pKa = 10.28 ASRR470 pKa = 11.84 TLNEE474 pKa = 3.98 SEE476 pKa = 4.93 LNYY479 pKa = 10.11 STIEE483 pKa = 4.03 KK484 pKa = 9.98 EE485 pKa = 3.78 LLAIVWATKK494 pKa = 8.79 YY495 pKa = 9.54 FRR497 pKa = 11.84 PYY499 pKa = 10.58 LFGRR503 pKa = 11.84 KK504 pKa = 8.29 FKK506 pKa = 10.68 ILTDD510 pKa = 3.85 HH511 pKa = 7.48 KK512 pKa = 10.1 PLQWMMNLKK521 pKa = 10.37 DD522 pKa = 3.71 PNSRR526 pKa = 11.84 MTRR529 pKa = 11.84 WRR531 pKa = 11.84 LRR533 pKa = 11.84 LSEE536 pKa = 4.09 YY537 pKa = 10.83 DD538 pKa = 3.36 FSVVYY543 pKa = 10.5 KK544 pKa = 10.44 KK545 pKa = 11.03 GKK547 pKa = 10.39 SNTNADD553 pKa = 3.32 ALSRR557 pKa = 11.84 VEE559 pKa = 3.78 IHH561 pKa = 5.08 TTEE564 pKa = 3.63 IDD566 pKa = 3.86 EE567 pKa = 4.32 IDD569 pKa = 3.89 SVIEE573 pKa = 4.14 NIKK576 pKa = 10.15 EE577 pKa = 3.83 LSSMINNPSEE587 pKa = 3.78 TSRR590 pKa = 11.84 PQRR593 pKa = 11.84 QTEE596 pKa = 4.01 NTDD599 pKa = 3.55 EE600 pKa = 3.99 IRR602 pKa = 11.84 QNSTTDD608 pKa = 3.23 TVHH611 pKa = 6.7 TSDD614 pKa = 3.51 EE615 pKa = 4.35 HH616 pKa = 6.86 PILEE620 pKa = 4.24 VPITNEE626 pKa = 3.36 PLNRR630 pKa = 11.84 FHH632 pKa = 7.05 RR633 pKa = 11.84 QIHH636 pKa = 5.09 LTVVGDD642 pKa = 3.61 IKK644 pKa = 10.69 RR645 pKa = 11.84 RR646 pKa = 11.84 PIVTKK651 pKa = 9.73 PFEE654 pKa = 4.1 SHH656 pKa = 4.55 TRR658 pKa = 11.84 IAIQLSEE665 pKa = 4.67 SNLEE669 pKa = 3.67 QDD671 pKa = 3.84 VISAIKK677 pKa = 10.1 EE678 pKa = 3.98 YY679 pKa = 11.26 VNPKK683 pKa = 10.0 VKK685 pKa = 9.34 TALIINPPLKK695 pKa = 9.3 MYY697 pKa = 10.4 SIIPIIQKK705 pKa = 7.24 TFRR708 pKa = 11.84 SSSLNLVLTKK718 pKa = 10.65 VEE720 pKa = 4.09 LEE722 pKa = 4.05 NVKK725 pKa = 10.45 EE726 pKa = 3.98 YY727 pKa = 10.95 LRR729 pKa = 11.84 QQDD732 pKa = 4.15 IIRR735 pKa = 11.84 HH736 pKa = 4.3 YY737 pKa = 11.13 HH738 pKa = 6.3 DD739 pKa = 4.28 GKK741 pKa = 9.18 TNHH744 pKa = 6.77 RR745 pKa = 11.84 GINEE749 pKa = 4.29 CYY751 pKa = 9.49 LALSKK756 pKa = 10.42 RR757 pKa = 11.84 YY758 pKa = 8.28 YY759 pKa = 8.04 WPRR762 pKa = 11.84 MKK764 pKa = 10.75 DD765 pKa = 3.21 QITKK769 pKa = 10.02 FINEE773 pKa = 4.21 CTICGQAKK781 pKa = 9.97 YY782 pKa = 10.1 DD783 pKa = 3.87 RR784 pKa = 11.84 NPIRR788 pKa = 11.84 PQFNIVPPATKK799 pKa = 9.91 PLEE802 pKa = 4.33 TVHH805 pKa = 6.58 MDD807 pKa = 3.82 LFTVQNEE814 pKa = 4.24 KK815 pKa = 11.02 YY816 pKa = 8.74 ITFIDD821 pKa = 3.79 VFTKK825 pKa = 10.67 YY826 pKa = 10.26 GQAYY830 pKa = 9.37 HH831 pKa = 6.72 LRR833 pKa = 11.84 DD834 pKa = 3.34 GTAISILQALLRR846 pKa = 11.84 FCTHH850 pKa = 6.68 HH851 pKa = 6.7 GLPITIVTDD860 pKa = 3.12 NGTEE864 pKa = 3.95 FSNQLFSEE872 pKa = 5.06 FVRR875 pKa = 11.84 IHH877 pKa = 6.61 KK878 pKa = 9.97 IIHH881 pKa = 6.41 HH882 pKa = 6.05 KK883 pKa = 8.7 TLPHH887 pKa = 6.26 SPSDD891 pKa = 3.25 NGNIEE896 pKa = 4.37 RR897 pKa = 11.84 FHH899 pKa = 5.67 STILEE904 pKa = 4.36 HH905 pKa = 8.01 IRR907 pKa = 11.84 ILKK910 pKa = 8.99 LQHH913 pKa = 6.37 KK914 pKa = 9.76 DD915 pKa = 3.41 EE916 pKa = 5.77 PIVNLMPYY924 pKa = 10.25 AIIGYY929 pKa = 9.07 NSSIHH934 pKa = 6.66 SFTKK938 pKa = 10.26 CRR940 pKa = 11.84 PFDD943 pKa = 3.9 LLNGHH948 pKa = 6.76 FDD950 pKa = 3.54 PRR952 pKa = 11.84 DD953 pKa = 3.62 PLDD956 pKa = 3.81 IDD958 pKa = 3.7 LTEE961 pKa = 5.91 HH962 pKa = 7.57 ILQQYY967 pKa = 8.17 AQNHH971 pKa = 4.75 RR972 pKa = 11.84 QQMKK976 pKa = 9.39 QVYY979 pKa = 9.08 EE980 pKa = 4.42 IINEE984 pKa = 3.93 TSLANRR990 pKa = 11.84 TALIEE995 pKa = 4.3 SRR997 pKa = 11.84 NKK999 pKa = 8.59 TRR1001 pKa = 11.84 EE1002 pKa = 3.98 SEE1004 pKa = 4.15 VEE1006 pKa = 4.26 YY1007 pKa = 10.49 IPQQQVFIKK1016 pKa = 10.5 NPLASRR1022 pKa = 11.84 QKK1024 pKa = 9.15 VAPRR1028 pKa = 11.84 YY1029 pKa = 6.94 TQDD1032 pKa = 2.87 TVLADD1037 pKa = 4.19 LPIHH1041 pKa = 7.03 IYY1043 pKa = 8.5 TSKK1046 pKa = 10.68 KK1047 pKa = 9.3 RR1048 pKa = 11.84 GPVAKK1053 pKa = 10.21 ARR1055 pKa = 11.84 LKK1057 pKa = 10.42 RR1058 pKa = 11.84 VPKK1061 pKa = 10.72 GNTLLQDD1068 pKa = 3.6 SAATDD1073 pKa = 3.71 NTCDD1077 pKa = 3.17 ASSRR1081 pKa = 11.84 DD1082 pKa = 3.55 KK1083 pKa = 10.97 TT1084 pKa = 3.75
Molecular weight: 125.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.497
IPC2_protein 7.863
IPC_protein 7.688
Toseland 7.571
ProMoST 8.273
Dawson 8.492
Bjellqvist 8.697
Wikipedia 8.448
Rodwell 8.551
Grimsley 7.673
Solomon 8.536
Lehninger 8.536
Nozaki 8.843
DTASelect 8.492
Thurlkill 8.58
EMBOSS 8.668
Sillero 8.843
Patrickios 4.482
IPC_peptide 8.521
IPC2_peptide 7.497
IPC2.peptide.svr19 7.782
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1906
410
1084
635.3
73.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.092 ± 0.101
1.574 ± 0.108
5.456 ± 0.52
6.139 ± 0.098
3.83 ± 0.181
3.253 ± 0.486
2.833 ± 0.311
7.975 ± 1.313
6.716 ± 0.882
9.286 ± 0.988
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.941 ± 0.22
5.981 ± 0.875
6.558 ± 1.684
5.247 ± 0.388
5.247 ± 0.74
6.716 ± 0.208
6.821 ± 0.165
5.299 ± 0.347
0.577 ± 0.098
4.46 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here