Flavobacteriales bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; unclassified Flavobacteriales

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q3LXI3|A0A4Q3LXI3_9FLAO RagB/SusD family nutrient uptake outer membrane protein OS=Flavobacteriales bacterium OX=2021391 GN=EOO42_08670 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.5NLMKK6 pKa = 10.17IVSCFLILSGIFARR20 pKa = 11.84AQADD24 pKa = 3.52PDD26 pKa = 4.23LEE28 pKa = 4.63ISTNSNTANGPAQSYY43 pKa = 7.7TLNFLKK49 pKa = 10.0DD50 pKa = 3.54TQNPTNVTAGPTPFVTAPNPLSVTLSISNSQYY82 pKa = 9.7ATAVTADD89 pKa = 4.27PLTQNALNLGYY100 pKa = 10.21SAGVPATTTLLSSGNGSVAANFTYY124 pKa = 10.2SGYY127 pKa = 10.85ANSAIGINPSGSNIASIRR145 pKa = 11.84NTGVQLMVATNQFSYY160 pKa = 11.25APYY163 pKa = 10.58KK164 pKa = 10.62KK165 pKa = 9.39LTNAKK170 pKa = 9.53IYY172 pKa = 10.35MGDD175 pKa = 3.23LTFTFSRR182 pKa = 11.84PVNNPVLHH190 pKa = 6.01FAGLGGSLGNQTLKK204 pKa = 10.39TSFEE208 pKa = 5.07LIGSAVALSRR218 pKa = 11.84LSGVPHH224 pKa = 6.97FDD226 pKa = 2.89VTSNVISSTSTGLLNTGNGNGSVLVTGRR254 pKa = 11.84GITSVSFKK262 pKa = 10.62IYY264 pKa = 9.31VTGDD268 pKa = 3.38GLNGADD274 pKa = 3.96ASTGYY279 pKa = 9.62TSTDD283 pKa = 2.43AWATNASGIFLPNNGTGGDD302 pKa = 3.9LMVISASLGEE312 pKa = 4.41SDD314 pKa = 5.26LSVTKK319 pKa = 10.01TVTNDD324 pKa = 3.29TPVVGSNVTFNIAATNSGMSKK345 pKa = 10.17SPEE348 pKa = 4.11TKK350 pKa = 9.47VTDD353 pKa = 4.37LLPSGFTYY361 pKa = 10.65VSSTTSVGTYY371 pKa = 10.64NSVTGIWDD379 pKa = 3.32IGEE382 pKa = 4.09MAIGAIATMSITATVKK398 pKa = 10.58SSGIHH403 pKa = 5.86TNTATIAGHH412 pKa = 5.48NTDD415 pKa = 4.72SNLNNNTASALVQQDD430 pKa = 3.0SDD432 pKa = 3.91GDD434 pKa = 4.27GVPDD438 pKa = 3.92TTDD441 pKa = 4.51LDD443 pKa = 4.3DD444 pKa = 6.11DD445 pKa = 4.44NDD447 pKa = 5.06GILDD451 pKa = 3.63SSEE454 pKa = 4.62NGPCTATSPLSSTGWKK470 pKa = 8.05ATVYY474 pKa = 9.59DD475 pKa = 4.55APSSTDD481 pKa = 2.53AWGEE485 pKa = 3.59ISAATTFPTASYY497 pKa = 10.68YY498 pKa = 10.28PIATFDD504 pKa = 3.7YY505 pKa = 11.05NEE507 pKa = 4.01FAGTSDD513 pKa = 3.96AFDD516 pKa = 4.35FNFATGAIGLKK527 pKa = 9.92AANPKK532 pKa = 7.28VTNYY536 pKa = 10.33VGGTIDD542 pKa = 3.7GNAFGSPVNTQDD554 pKa = 4.54DD555 pKa = 4.32YY556 pKa = 12.04AIMFRR561 pKa = 11.84KK562 pKa = 8.45TITAQEE568 pKa = 4.01AGTYY572 pKa = 10.69AFDD575 pKa = 4.27FLYY578 pKa = 11.0GDD580 pKa = 4.29DD581 pKa = 4.13QLFIYY586 pKa = 10.36KK587 pKa = 10.29NGVKK591 pKa = 10.19VYY593 pKa = 10.8QLLQAYY599 pKa = 7.51NVPATNNAATITVVEE614 pKa = 4.16GDD616 pKa = 4.23VISMLVAEE624 pKa = 4.49EE625 pKa = 4.33NTWNTEE631 pKa = 2.98IRR633 pKa = 11.84FNGRR637 pKa = 11.84KK638 pKa = 8.84IAGTCTLTDD647 pKa = 3.45TDD649 pKa = 4.53GDD651 pKa = 4.45GIPNYY656 pKa = 10.62LDD658 pKa = 4.76LDD660 pKa = 4.14SDD662 pKa = 4.4NDD664 pKa = 3.76GCTDD668 pKa = 3.52ARR670 pKa = 11.84EE671 pKa = 4.04GDD673 pKa = 4.04EE674 pKa = 4.99NVLASQLVTASGTLTVGTGSTASNQNLGITVDD706 pKa = 3.64ANGVPTLVNSGGAADD721 pKa = 4.08IGADD725 pKa = 3.34QGQGIGVSQDD735 pKa = 3.27SAKK738 pKa = 10.52SDD740 pKa = 3.97CLDD743 pKa = 3.41SDD745 pKa = 4.2GDD747 pKa = 4.44GVPDD751 pKa = 4.49WSDD754 pKa = 4.26LDD756 pKa = 4.45DD757 pKa = 5.87DD758 pKa = 4.93NDD760 pKa = 5.02GILDD764 pKa = 4.03TEE766 pKa = 4.58EE767 pKa = 4.61LRR769 pKa = 11.84CDD771 pKa = 3.99YY772 pKa = 10.93NPSVYY777 pKa = 10.81NFVSGSGAIRR787 pKa = 11.84NQLLIFDD794 pKa = 4.03WTGASLTNVNDD805 pKa = 3.58TFTTSKK811 pKa = 8.92TVNGITYY818 pKa = 7.21TAKK821 pKa = 9.23ATVVEE826 pKa = 4.41LVGGYY831 pKa = 10.05YY832 pKa = 10.19SGQSPIPSILNTFPGGGNQMFWRR855 pKa = 11.84YY856 pKa = 10.12ANYY859 pKa = 8.0GTTATDD865 pKa = 3.76DD866 pKa = 3.45LRR868 pKa = 11.84GAIVFQLARR877 pKa = 11.84VANNKK882 pKa = 9.92LSIKK886 pKa = 10.29FDD888 pKa = 3.01ITATKK893 pKa = 10.49GGLPQQFDD901 pKa = 4.53AIAFDD906 pKa = 4.34AEE908 pKa = 4.49STTTAEE914 pKa = 4.11KK915 pKa = 10.94GLYY918 pKa = 5.92TTNAADD924 pKa = 3.16WTLIDD929 pKa = 4.12NLGGVPTATEE939 pKa = 3.62ASIVGKK945 pKa = 7.36QLRR948 pKa = 11.84YY949 pKa = 10.53DD950 pKa = 3.78DD951 pKa = 4.16TQTAQLSLFAYY962 pKa = 8.13TRR964 pKa = 11.84GVNVSILGEE973 pKa = 4.09YY974 pKa = 10.71SNEE977 pKa = 3.95TNLARR982 pKa = 11.84AQAMGVGVYY991 pKa = 9.21LYY993 pKa = 10.83CDD995 pKa = 3.88ADD997 pKa = 3.55NDD999 pKa = 4.17GTFNYY1004 pKa = 10.53LDD1006 pKa = 4.84LDD1008 pKa = 4.1SDD1010 pKa = 4.42NDD1012 pKa = 3.86GCLDD1016 pKa = 3.57ALEE1019 pKa = 5.16GGSALAISNLVNASGTVSVGTGSTASNQNLCASSSCVGSTGIPTIVNQTSGQTGGSSTNNLVKK1082 pKa = 10.51AAVCSVCYY1090 pKa = 9.44EE1091 pKa = 4.29LPTDD1095 pKa = 4.27LTTSVPVSHH1104 pKa = 7.34GITVV1108 pKa = 3.5

Molecular weight: 114.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q3LPY7|A0A4Q3LPY7_9FLAO Malonyl CoA-acyl carrier protein transacylase OS=Flavobacteriales bacterium OX=2021391 GN=fabD PE=3 SV=1
MM1 pKa = 7.32NALSPTKK8 pKa = 10.14TIMRR12 pKa = 11.84TDD14 pKa = 2.9ITLTTEE20 pKa = 3.91RR21 pKa = 11.84KK22 pKa = 7.2TPYY25 pKa = 8.81NTGFASGGLTCKK37 pKa = 10.63LGALCFYY44 pKa = 10.27LSSVLADD51 pKa = 3.45SFVLRR56 pKa = 11.84SPPEE60 pKa = 3.56RR61 pKa = 11.84KK62 pKa = 9.37ARR64 pKa = 11.84NRR66 pKa = 11.84WRR68 pKa = 3.48

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4745

0

4745

1671011

22

3715

352.2

39.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.721 ± 0.038

0.679 ± 0.012

5.251 ± 0.023

5.144 ± 0.037

4.834 ± 0.027

7.233 ± 0.037

1.453 ± 0.02

7.071 ± 0.032

6.844 ± 0.046

9.054 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.981 ± 0.02

6.582 ± 0.043

3.678 ± 0.02

3.603 ± 0.021

3.594 ± 0.024

6.701 ± 0.034

6.59 ± 0.063

6.594 ± 0.033

1.122 ± 0.014

4.27 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski