Amino acid dipepetide frequency for Flavobacteriales bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.098AlaAla: 6.098 ± 0.078	0.592AlaCys: 0.592 ± 0.021	4.412AlaAsp: 4.412 ± 0.054	4.08AlaGlu: 4.08 ± 0.052	3.427AlaPhe: 3.427 ± 0.046	5.83AlaGly: 5.83 ± 0.072	1.005AlaHis: 1.005 ± 0.023	5.529AlaIle: 5.529 ± 0.06	5.263AlaLys: 5.263 ± 0.077	6.692AlaLeu: 6.692 ± 0.082	1.587AlaMet: 1.587 ± 0.034	5.139AlaAsn: 5.139 ± 0.072	2.494AlaPro: 2.494 ± 0.042	3.166AlaGln: 3.166 ± 0.046	2.294AlaArg: 2.294 ± 0.04	5.3AlaSer: 5.3 ± 0.063	5.369AlaThr: 5.369 ± 0.095	5.023AlaVal: 5.023 ± 0.061	0.728AlaTrp: 0.728 ± 0.023	3.182AlaTyr: 3.182 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.476CysAla: 0.476 ± 0.018	0.101CysCys: 0.101 ± 0.008	0.321CysAsp: 0.321 ± 0.014	0.361CysGlu: 0.361 ± 0.016	0.329CysPhe: 0.329 ± 0.015	0.585CysGly: 0.585 ± 0.026	0.131CysHis: 0.131 ± 0.009	0.552CysIle: 0.552 ± 0.02	0.486CysLys: 0.486 ± 0.017	0.636CysLeu: 0.636 ± 0.022	0.132CysMet: 0.132 ± 0.009	0.391CysAsn: 0.391 ± 0.017	0.26CysPro: 0.26 ± 0.014	0.182CysGln: 0.182 ± 0.01	0.212CysArg: 0.212 ± 0.011	0.483CysSer: 0.483 ± 0.019	0.426CysThr: 0.426 ± 0.025	0.42CysVal: 0.42 ± 0.016	0.053CysTrp: 0.053 ± 0.005	0.253CysTyr: 0.253 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.191AspAla: 4.191 ± 0.047	0.318AspCys: 0.318 ± 0.014	2.497AspAsp: 2.497 ± 0.047	3.077AspGlu: 3.077 ± 0.056	3.142AspPhe: 3.142 ± 0.043	4.32AspGly: 4.32 ± 0.059	0.792AspHis: 0.792 ± 0.027	3.8AspIle: 3.8 ± 0.052	3.588AspLys: 3.588 ± 0.054	5.01AspLeu: 5.01 ± 0.058	1.002AspMet: 1.002 ± 0.027	2.898AspAsn: 2.898 ± 0.045	2.084AspPro: 2.084 ± 0.035	1.794AspGln: 1.794 ± 0.031	2.065AspArg: 2.065 ± 0.034	2.548AspSer: 2.548 ± 0.048	2.34AspThr: 2.34 ± 0.041	3.701AspVal: 3.701 ± 0.048	0.771AspTrp: 0.771 ± 0.023	2.576AspTyr: 2.576 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	3.861GluAla: 3.861 ± 0.058	0.272GluCys: 0.272 ± 0.011	2.518GluAsp: 2.518 ± 0.039	3.233GluGlu: 3.233 ± 0.068	2.248GluPhe: 2.248 ± 0.041	3.111GluGly: 3.111 ± 0.043	0.837GluHis: 0.837 ± 0.024	4.113GluIle: 4.113 ± 0.054	4.006GluLys: 4.006 ± 0.064	5.118GluLeu: 5.118 ± 0.073	1.175GluMet: 1.175 ± 0.03	3.451GluAsn: 3.451 ± 0.054	1.439GluPro: 1.439 ± 0.031	2.137GluGln: 2.137 ± 0.04	2.276GluArg: 2.276 ± 0.049	2.571GluSer: 2.571 ± 0.04	2.906GluThr: 2.906 ± 0.046	3.745GluVal: 3.745 ± 0.046	0.576GluTrp: 0.576 ± 0.018	1.847GluTyr: 1.847 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.63PheAla: 3.63 ± 0.048	0.396PheCys: 0.396 ± 0.015	2.814PheAsp: 2.814 ± 0.042	2.531PheGlu: 2.531 ± 0.038	2.227PhePhe: 2.227 ± 0.042	3.758PheGly: 3.758 ± 0.054	0.609PheHis: 0.609 ± 0.02	3.102PheIle: 3.102 ± 0.046	3.249PheLys: 3.249 ± 0.051	4.029PheLeu: 4.029 ± 0.056	0.959PheMet: 0.959 ± 0.026	3.432PheAsn: 3.432 ± 0.048	1.623PhePro: 1.623 ± 0.031	1.317PheGln: 1.317 ± 0.028	1.728PheArg: 1.728 ± 0.036	3.908PheSer: 3.908 ± 0.055	3.457PheThr: 3.457 ± 0.053	2.975PheVal: 2.975 ± 0.046	0.51PheTrp: 0.51 ± 0.022	2.089PheTyr: 2.089 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.378GlyAla: 5.378 ± 0.081	0.653GlyCys: 0.653 ± 0.033	3.449GlyAsp: 3.449 ± 0.045	3.186GlyGlu: 3.186 ± 0.05	3.748GlyPhe: 3.748 ± 0.052	5.536GlyGly: 5.536 ± 0.089	1.02GlyHis: 1.02 ± 0.027	5.319GlyIle: 5.319 ± 0.058	5.271GlyLys: 5.271 ± 0.062	6.406GlyLeu: 6.406 ± 0.072	1.565GlyMet: 1.565 ± 0.03	4.762GlyAsn: 4.762 ± 0.075	1.539GlyPro: 1.539 ± 0.036	2.322GlyGln: 2.322 ± 0.043	2.441GlyArg: 2.441 ± 0.043	5.106GlySer: 5.106 ± 0.073	5.356GlyThr: 5.356 ± 0.101	4.965GlyVal: 4.965 ± 0.066	0.901GlyTrp: 0.901 ± 0.024	3.4GlyTyr: 3.4 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	0.916HisAla: 0.916 ± 0.026	0.147HisCys: 0.147 ± 0.01	0.685HisAsp: 0.685 ± 0.021	0.791HisGlu: 0.791 ± 0.026	0.857HisPhe: 0.857 ± 0.024	0.931HisGly: 0.931 ± 0.024	0.397HisHis: 0.397 ± 0.017	1.127HisIle: 1.127 ± 0.03	0.871HisLys: 0.871 ± 0.024	1.495HisLeu: 1.495 ± 0.035	0.259HisMet: 0.259 ± 0.014	0.787HisAsn: 0.787 ± 0.023	0.71HisPro: 0.71 ± 0.026	0.674HisGln: 0.674 ± 0.022	0.601HisArg: 0.601 ± 0.02	0.874HisSer: 0.874 ± 0.025	0.793HisThr: 0.793 ± 0.025	0.752HisVal: 0.752 ± 0.022	0.19HisTrp: 0.19 ± 0.012	0.676HisTyr: 0.676 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.805IleAla: 5.805 ± 0.077	0.619IleCys: 0.619 ± 0.022	4.255IleAsp: 4.255 ± 0.052	3.964IleGlu: 3.964 ± 0.051	2.963IlePhe: 2.963 ± 0.048	4.983IleGly: 4.983 ± 0.062	0.969IleHis: 0.969 ± 0.026	4.735IleIle: 4.735 ± 0.071	4.937IleLys: 4.937 ± 0.067	5.715IleLeu: 5.715 ± 0.075	1.213IleMet: 1.213 ± 0.03	4.904IleAsn: 4.904 ± 0.061	2.868IlePro: 2.868 ± 0.041	2.145IleGln: 2.145 ± 0.035	2.577IleArg: 2.577 ± 0.044	5.238IleSer: 5.238 ± 0.061	5.075IleThr: 5.075 ± 0.071	4.321IleVal: 4.321 ± 0.057	0.659IleTrp: 0.659 ± 0.021	2.767IleTyr: 2.767 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	5.18LysAla: 5.18 ± 0.066	0.267LysCys: 0.267 ± 0.013	3.96LysAsp: 3.96 ± 0.059	4.016LysGlu: 4.016 ± 0.064	2.81LysPhe: 2.81 ± 0.041	4.52LysGly: 4.52 ± 0.054	1.137LysHis: 1.137 ± 0.029	4.978LysIle: 4.978 ± 0.064	5.029LysLys: 5.029 ± 0.065	6.216LysLeu: 6.216 ± 0.077	1.608LysMet: 1.608 ± 0.033	4.437LysAsn: 4.437 ± 0.06	2.583LysPro: 2.583 ± 0.049	2.621LysGln: 2.621 ± 0.05	2.452LysArg: 2.452 ± 0.041	4.029LysSer: 4.029 ± 0.049	4.317LysThr: 4.317 ± 0.055	4.616LysVal: 4.616 ± 0.057	0.813LysTrp: 0.813 ± 0.023	2.847LysTyr: 2.847 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	7.321LeuAla: 7.321 ± 0.086	0.627LeuCys: 0.627 ± 0.021	4.642LeuAsp: 4.642 ± 0.057	4.398LeuGlu: 4.398 ± 0.067	4.178LeuPhe: 4.178 ± 0.059	5.879LeuGly: 5.879 ± 0.064	1.314LeuHis: 1.314 ± 0.035	5.986LeuIle: 5.986 ± 0.078	6.633LeuLys: 6.633 ± 0.078	8.338LeuLeu: 8.338 ± 0.097	1.852LeuMet: 1.852 ± 0.032	6.264LeuAsn: 6.264 ± 0.074	3.962LeuPro: 3.962 ± 0.048	3.288LeuGln: 3.288 ± 0.046	3.449LeuArg: 3.449 ± 0.05	6.905LeuSer: 6.905 ± 0.08	6.049LeuThr: 6.049 ± 0.065	5.484LeuVal: 5.484 ± 0.059	0.807LeuTrp: 0.807 ± 0.024	3.167LeuTyr: 3.167 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	1.6MetAla: 1.6 ± 0.034	0.111MetCys: 0.111 ± 0.008	1.0MetAsp: 1.0 ± 0.028	1.022MetGlu: 1.022 ± 0.028	0.786MetPhe: 0.786 ± 0.025	1.377MetGly: 1.377 ± 0.034	0.318MetHis: 0.318 ± 0.013	1.318MetIle: 1.318 ± 0.031	1.789MetLys: 1.789 ± 0.032	1.88MetLeu: 1.88 ± 0.034	0.512MetMet: 0.512 ± 0.019	1.114MetAsn: 1.114 ± 0.023	0.921MetPro: 0.921 ± 0.027	0.829MetGln: 0.829 ± 0.023	0.828MetArg: 0.828 ± 0.022	1.225MetSer: 1.225 ± 0.029	0.953MetThr: 0.953 ± 0.026	1.379MetVal: 1.379 ± 0.031	0.186MetTrp: 0.186 ± 0.012	0.659MetTyr: 0.659 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	5.019AsnAla: 5.019 ± 0.055	0.403AsnCys: 0.403 ± 0.02	3.194AsnAsp: 3.194 ± 0.052	3.085AsnGlu: 3.085 ± 0.046	3.263AsnPhe: 3.263 ± 0.051	5.205AsnGly: 5.205 ± 0.085	0.87AsnHis: 0.87 ± 0.024	4.726AsnIle: 4.726 ± 0.057	3.867AsnLys: 3.867 ± 0.053	5.881AsnLeu: 5.881 ± 0.064	1.172AsnMet: 1.172 ± 0.026	4.348AsnAsn: 4.348 ± 0.068	3.132AsnPro: 3.132 ± 0.055	2.309AsnGln: 2.309 ± 0.036	2.449AsnArg: 2.449 ± 0.046	4.266AsnSer: 4.266 ± 0.065	4.257AsnThr: 4.257 ± 0.066	4.273AsnVal: 4.273 ± 0.061	0.853AsnTrp: 0.853 ± 0.023	3.248AsnTyr: 3.248 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	3.018ProAla: 3.018 ± 0.044	0.198ProCys: 0.198 ± 0.012	2.044ProAsp: 2.044 ± 0.031	2.294ProGlu: 2.294 ± 0.038	1.761ProPhe: 1.761 ± 0.034	2.619ProGly: 2.619 ± 0.042	0.526ProHis: 0.526 ± 0.019	2.642ProIle: 2.642 ± 0.039	2.228ProLys: 2.228 ± 0.043	3.198ProLeu: 3.198 ± 0.047	0.696ProMet: 0.696 ± 0.023	2.529ProAsn: 2.529 ± 0.042	0.856ProPro: 0.856 ± 0.026	1.374ProGln: 1.374 ± 0.027	1.069ProArg: 1.069 ± 0.026	2.501ProSer: 2.501 ± 0.041	2.588ProThr: 2.588 ± 0.051	2.789ProVal: 2.789 ± 0.048	0.339ProTrp: 0.339 ± 0.012	1.516ProTyr: 1.516 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	2.576GlnAla: 2.576 ± 0.034	0.167GlnCys: 0.167 ± 0.01	1.618GlnAsp: 1.618 ± 0.027	1.789GlnGlu: 1.789 ± 0.032	1.678GlnPhe: 1.678 ± 0.032	2.173GlnGly: 2.173 ± 0.04	0.611GlnHis: 0.611 ± 0.02	2.577GlnIle: 2.577 ± 0.041	2.424GlnLys: 2.424 ± 0.038	3.692GlnLeu: 3.692 ± 0.054	0.79GlnMet: 0.79 ± 0.022	2.385GlnAsn: 2.385 ± 0.038	1.391GlnPro: 1.391 ± 0.033	1.846GlnGln: 1.846 ± 0.037	1.373GlnArg: 1.373 ± 0.029	2.227GlnSer: 2.227 ± 0.033	2.471GlnThr: 2.471 ± 0.042	2.3GlnVal: 2.3 ± 0.034	0.426GlnTrp: 0.426 ± 0.016	1.515GlnTyr: 1.515 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.385ArgAla: 2.385 ± 0.036	0.206ArgCys: 0.206 ± 0.011	1.836ArgAsp: 1.836 ± 0.034	1.795ArgGlu: 1.795 ± 0.036	1.947ArgPhe: 1.947 ± 0.035	2.264ArgGly: 2.264 ± 0.041	0.466ArgHis: 0.466 ± 0.018	2.8ArgIle: 2.8 ± 0.045	2.595ArgLys: 2.595 ± 0.038	3.504ArgLeu: 3.504 ± 0.05	0.853ArgMet: 0.853 ± 0.023	2.425ArgAsn: 2.425 ± 0.039	1.253ArgPro: 1.253 ± 0.033	1.252ArgGln: 1.252 ± 0.029	1.382ArgArg: 1.382 ± 0.035	2.197ArgSer: 2.197 ± 0.042	2.169ArgThr: 2.169 ± 0.041	2.276ArgVal: 2.276 ± 0.039	0.545ArgTrp: 0.545 ± 0.017	1.784ArgTyr: 1.784 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	5.177SerAla: 5.177 ± 0.058	0.566SerCys: 0.566 ± 0.018	3.165SerAsp: 3.165 ± 0.04	2.972SerGlu: 2.972 ± 0.045	3.865SerPhe: 3.865 ± 0.053	5.416SerGly: 5.416 ± 0.081	0.923SerHis: 0.923 ± 0.03	4.913SerIle: 4.913 ± 0.058	4.106SerLys: 4.106 ± 0.053	6.191SerLeu: 6.191 ± 0.067	1.199SerMet: 1.199 ± 0.027	4.181SerAsn: 4.181 ± 0.055	2.35SerPro: 2.35 ± 0.039	2.111SerGln: 2.111 ± 0.035	2.201SerArg: 2.201 ± 0.036	4.728SerSer: 4.728 ± 0.067	4.445SerThr: 4.445 ± 0.069	4.58SerVal: 4.58 ± 0.057	0.735SerTrp: 0.735 ± 0.021	3.182SerTyr: 3.182 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	5.49ThrAla: 5.49 ± 0.09	0.333ThrCys: 0.333 ± 0.019	3.497ThrAsp: 3.497 ± 0.048	3.038ThrGlu: 3.038 ± 0.047	3.191ThrPhe: 3.191 ± 0.055	5.439ThrGly: 5.439 ± 0.108	0.868ThrHis: 0.868 ± 0.023	4.813ThrIle: 4.813 ± 0.075	3.823ThrLys: 3.823 ± 0.053	5.955ThrLeu: 5.955 ± 0.063	0.927ThrMet: 0.927 ± 0.025	4.089ThrAsn: 4.089 ± 0.071	2.81ThrPro: 2.81 ± 0.051	2.181ThrGln: 2.181 ± 0.038	1.904ThrArg: 1.904 ± 0.032	4.422ThrSer: 4.422 ± 0.074	4.715ThrThr: 4.715 ± 0.097	4.651ThrVal: 4.651 ± 0.081	0.728ThrTrp: 0.728 ± 0.02	3.024ThrTyr: 3.024 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	5.203ValAla: 5.203 ± 0.067	0.457ValCys: 0.457 ± 0.018	3.594ValAsp: 3.594 ± 0.052	3.323ValGlu: 3.323 ± 0.048	3.155ValPhe: 3.155 ± 0.041	4.487ValGly: 4.487 ± 0.062	0.81ValHis: 0.81 ± 0.026	4.539ValIle: 4.539 ± 0.057	4.726ValLys: 4.726 ± 0.062	5.801ValLeu: 5.801 ± 0.064	1.273ValMet: 1.273 ± 0.03	4.477ValAsn: 4.477 ± 0.061	2.524ValPro: 2.524 ± 0.035	2.055ValGln: 2.055 ± 0.036	2.251ValArg: 2.251 ± 0.04	4.831ValSer: 4.831 ± 0.062	4.518ValThr: 4.518 ± 0.083	4.691ValVal: 4.691 ± 0.072	0.699ValTrp: 0.699 ± 0.02	2.53ValTyr: 2.53 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.738TrpAla: 0.738 ± 0.024	0.084TrpCys: 0.084 ± 0.007	0.682TrpAsp: 0.682 ± 0.023	0.589TrpGlu: 0.589 ± 0.019	0.54TrpPhe: 0.54 ± 0.022	0.838TrpGly: 0.838 ± 0.023	0.204TrpHis: 0.204 ± 0.011	0.667TrpIle: 0.667 ± 0.019	0.752TrpLys: 0.752 ± 0.023	1.023TrpLeu: 1.023 ± 0.028	0.322TrpMet: 0.322 ± 0.015	0.718TrpAsn: 0.718 ± 0.021	0.322TrpPro: 0.322 ± 0.014	0.53TrpGln: 0.53 ± 0.017	0.493TrpArg: 0.493 ± 0.019	0.703TrpSer: 0.703 ± 0.02	0.728TrpThr: 0.728 ± 0.024	0.671TrpVal: 0.671 ± 0.02	0.16TrpTrp: 0.16 ± 0.01	0.46TrpTyr: 0.46 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.15TyrAla: 3.15 ± 0.046	0.271TyrCys: 0.271 ± 0.015	2.33TyrAsp: 2.33 ± 0.043	1.896TyrGlu: 1.896 ± 0.041	2.229TyrPhe: 2.229 ± 0.043	3.056TyrGly: 3.056 ± 0.047	0.725TyrHis: 0.725 ± 0.026	2.479TyrIle: 2.479 ± 0.037	2.793TyrLys: 2.793 ± 0.039	3.764TyrLeu: 3.764 ± 0.048	0.709TyrMet: 0.709 ± 0.02	3.088TyrAsn: 3.088 ± 0.056	1.662TyrPro: 1.662 ± 0.034	1.897TyrGln: 1.897 ± 0.035	1.889TyrArg: 1.889 ± 0.036	2.945TyrSer: 2.945 ± 0.05	2.967TyrThr: 2.967 ± 0.052	2.33TyrVal: 2.33 ± 0.035	0.541TyrTrp: 0.541 ± 0.019	1.982TyrTyr: 1.982 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4745 proteins (1671011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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