Klebsiella phage ST15-OXA48phi14.1
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482MFR2|A0A482MFR2_9CAUD SsrA-binding protein OS=Klebsiella phage ST15-OXA48phi14.1 OX=2510455 PE=3 SV=1
MM1 pKa = 7.61 AKK3 pKa = 9.95 VDD5 pKa = 4.1 LEE7 pKa = 4.23 KK8 pKa = 10.4 IIPVFALRR16 pKa = 11.84 IANVGDD22 pKa = 3.78 VTDD25 pKa = 4.72 GQCTLTIEE33 pKa = 4.78 GGQDD37 pKa = 2.98 VSDD40 pKa = 3.61 PVVVTEE46 pKa = 5.77 EE47 pKa = 4.43 YY48 pKa = 8.82 IQKK51 pKa = 10.31 YY52 pKa = 8.83 NPQPGGYY59 pKa = 10.37 YY60 pKa = 9.94 IMCSNGVGLYY70 pKa = 10.88 SNN72 pKa = 4.73
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.971
IPC2_protein 4.19
IPC_protein 4.012
Toseland 3.834
ProMoST 4.19
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.757
Solomon 3.986
Lehninger 3.935
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.139
Patrickios 0.401
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.042
Protein with the highest isoelectric point:
>tr|A0A482MGE7|A0A482MGE7_9CAUD Tail assembly protein OS=Klebsiella phage ST15-OXA48phi14.1 OX=2510455 PE=4 SV=1
MM1 pKa = 7.95 AEE3 pKa = 4.03 LQEE6 pKa = 3.86 VDD8 pKa = 3.08 AWLDD12 pKa = 3.31 ALLAEE17 pKa = 5.05 LEE19 pKa = 4.01 PAEE22 pKa = 4.91 RR23 pKa = 11.84 KK24 pKa = 10.21 RR25 pKa = 11.84 MMRR28 pKa = 11.84 DD29 pKa = 2.87 LAQQLRR35 pKa = 11.84 RR36 pKa = 11.84 SQQKK40 pKa = 9.63 NIRR43 pKa = 11.84 MQRR46 pKa = 11.84 NPDD49 pKa = 2.92 GTAYY53 pKa = 10.1 EE54 pKa = 4.13 PRR56 pKa = 11.84 RR57 pKa = 11.84 VTARR61 pKa = 11.84 AKK63 pKa = 9.44 QGRR66 pKa = 11.84 IRR68 pKa = 11.84 RR69 pKa = 11.84 QMFAKK74 pKa = 10.41 LRR76 pKa = 11.84 TTKK79 pKa = 10.36 YY80 pKa = 9.29 LKK82 pKa = 10.56 AVASQDD88 pKa = 3.55 SASVEE93 pKa = 4.1 FEE95 pKa = 4.01 SRR97 pKa = 11.84 VQRR100 pKa = 11.84 IARR103 pKa = 11.84 VHH105 pKa = 6.27 HH106 pKa = 5.92 YY107 pKa = 10.35 GLRR110 pKa = 11.84 DD111 pKa = 3.15 RR112 pKa = 11.84 VSRR115 pKa = 11.84 KK116 pKa = 9.95 GPEE119 pKa = 3.44 VKK121 pKa = 10.34 YY122 pKa = 10.89 AEE124 pKa = 4.16 RR125 pKa = 11.84 RR126 pKa = 11.84 LLGINDD132 pKa = 3.61 EE133 pKa = 4.71 SEE135 pKa = 5.02 DD136 pKa = 3.32 ITRR139 pKa = 11.84 DD140 pKa = 3.27 VLLRR144 pKa = 11.84 WLSQQ148 pKa = 2.99
Molecular weight: 17.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.589
IPC_protein 10.511
Toseland 10.701
ProMoST 10.452
Dawson 10.789
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 10.906
Grimsley 10.833
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.628
IPC_peptide 10.95
IPC2_peptide 9.326
IPC2.peptide.svr19 8.779
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
10425
39
875
226.6
25.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.564 ± 0.621
1.046 ± 0.175
6.053 ± 0.267
6.129 ± 0.331
3.319 ± 0.234
7.089 ± 0.508
2.005 ± 0.246
5.209 ± 0.248
5.266 ± 0.269
9.343 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.839 ± 0.182
4.336 ± 0.223
4.029 ± 0.189
4.278 ± 0.319
6.676 ± 0.411
5.928 ± 0.232
5.707 ± 0.327
6.619 ± 0.3
1.621 ± 0.154
2.945 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here