Lactobacillus sp. LL6
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1826 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A556UEG5|A0A556UEG5_9LACO IS200/IS605 family transposase OS=Lactobacillus sp. LL6 OX=2596827 GN=tnpA PE=4 SV=1
MM1 pKa = 7.13 QKK3 pKa = 10.4 YY4 pKa = 8.61 IKK6 pKa = 10.06 KK7 pKa = 10.78 GIGDD11 pKa = 3.68 WVSNNTGVVNVDD23 pKa = 2.85 NDD25 pKa = 3.94 TLEE28 pKa = 4.16 IATTQIDD35 pKa = 4.15 AYY37 pKa = 10.2 GDD39 pKa = 3.39 TVYY42 pKa = 10.97 CFVIDD47 pKa = 3.42 KK48 pKa = 10.74 GDD50 pKa = 3.62 YY51 pKa = 10.99 YY52 pKa = 10.95 EE53 pKa = 5.15 VSDD56 pKa = 5.28 DD57 pKa = 3.51 SHH59 pKa = 9.1 ILFKK63 pKa = 10.85 LDD65 pKa = 4.1 PGEE68 pKa = 4.24 TDD70 pKa = 2.97 ADD72 pKa = 4.24 LYY74 pKa = 10.48 RR75 pKa = 11.84 TGAQVAIGAGFNFDD89 pKa = 4.25 EE90 pKa = 5.03 DD91 pKa = 3.69 SCEE94 pKa = 3.95 IFVEE98 pKa = 4.11 VDD100 pKa = 3.17 KK101 pKa = 11.63 EE102 pKa = 4.04 NLAQAIIRR110 pKa = 11.84 LAQLQVAISYY120 pKa = 10.27 LGG122 pKa = 3.29
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.719
EMBOSS 3.834
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A556UEE4|A0A556UEE4_9LACO DUF805 domain-containing protein OS=Lactobacillus sp. LL6 OX=2596827 GN=FOD82_01535 PE=4 SV=1
MM1 pKa = 6.05 TTKK4 pKa = 9.88 RR5 pKa = 11.84 TYY7 pKa = 9.64 QPKK10 pKa = 8.89 KK11 pKa = 7.38 RR12 pKa = 11.84 HH13 pKa = 5.81 RR14 pKa = 11.84 SRR16 pKa = 11.84 VHH18 pKa = 6.84 GFMKK22 pKa = 10.5 RR23 pKa = 11.84 MSTSNGRR30 pKa = 11.84 KK31 pKa = 8.23 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.94 GRR41 pKa = 11.84 KK42 pKa = 8.67 VLSAA46 pKa = 4.05
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1826
0
1826
602995
17
5441
330.2
37.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.88 ± 0.072
0.485 ± 0.016
5.966 ± 0.088
5.762 ± 0.06
4.201 ± 0.062
6.414 ± 0.049
1.934 ± 0.027
7.834 ± 0.072
8.349 ± 0.063
8.894 ± 0.133
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.538 ± 0.044
6.098 ± 0.067
3.597 ± 0.077
3.756 ± 0.037
3.39 ± 0.054
6.139 ± 0.047
6.038 ± 0.132
6.748 ± 0.06
1.029 ± 0.022
3.948 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here