Amino acid dipepetide frequency for Lactobacillus sp. LL6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.431AlaAla: 5.431 ± 0.128
0.434AlaCys: 0.434 ± 0.031
3.879AlaAsp: 3.879 ± 0.088
3.748AlaGlu: 3.748 ± 0.091
2.726AlaPhe: 2.726 ± 0.095
4.922AlaGly: 4.922 ± 0.115
1.294AlaHis: 1.294 ± 0.045
5.693AlaIle: 5.693 ± 0.113
5.834AlaLys: 5.834 ± 0.12
6.063AlaLeu: 6.063 ± 0.124
1.781AlaMet: 1.781 ± 0.075
3.572AlaAsn: 3.572 ± 0.078
2.196AlaPro: 2.196 ± 0.065
2.904AlaGln: 2.904 ± 0.079
2.237AlaArg: 2.237 ± 0.077
4.27AlaSer: 4.27 ± 0.093
4.294AlaThr: 4.294 ± 0.097
4.534AlaVal: 4.534 ± 0.11
0.658AlaTrp: 0.658 ± 0.035
2.327AlaTyr: 2.327 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.323CysAla: 0.323 ± 0.025
0.05CysCys: 0.05 ± 0.008
0.305CysAsp: 0.305 ± 0.022
0.255CysGlu: 0.255 ± 0.02
0.24CysPhe: 0.24 ± 0.023
0.496CysGly: 0.496 ± 0.031
0.126CysHis: 0.126 ± 0.014
0.362CysIle: 0.362 ± 0.026
0.254CysLys: 0.254 ± 0.02
0.488CysLeu: 0.488 ± 0.033
0.123CysMet: 0.123 ± 0.014
0.207CysAsn: 0.207 ± 0.021
0.206CysPro: 0.206 ± 0.023
0.167CysGln: 0.167 ± 0.017
0.156CysArg: 0.156 ± 0.015
0.335CysSer: 0.335 ± 0.028
0.227CysThr: 0.227 ± 0.021
0.287CysVal: 0.287 ± 0.027
0.046CysTrp: 0.046 ± 0.007
0.194CysTyr: 0.194 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.751AspAla: 3.751 ± 0.098
0.247AspCys: 0.247 ± 0.024
3.348AspAsp: 3.348 ± 0.098
4.294AspGlu: 4.294 ± 0.104
2.708AspPhe: 2.708 ± 0.091
4.093AspGly: 4.093 ± 0.163
1.101AspHis: 1.101 ± 0.043
4.234AspIle: 4.234 ± 0.09
5.206AspLys: 5.206 ± 0.159
5.499AspLeu: 5.499 ± 0.111
1.421AspMet: 1.421 ± 0.047
3.6AspAsn: 3.6 ± 0.13
2.629AspPro: 2.629 ± 0.143
1.982AspGln: 1.982 ± 0.061
1.814AspArg: 1.814 ± 0.06
3.61AspSer: 3.61 ± 0.103
3.04AspThr: 3.04 ± 0.118
3.647AspVal: 3.647 ± 0.079
0.716AspTrp: 0.716 ± 0.038
2.723AspTyr: 2.723 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
3.993GluAla: 3.993 ± 0.098
0.209GluCys: 0.209 ± 0.02
3.431GluAsp: 3.431 ± 0.085
4.01GluGlu: 4.01 ± 0.099
2.149GluPhe: 2.149 ± 0.07
2.939GluGly: 2.939 ± 0.077
1.088GluHis: 1.088 ± 0.045
4.687GluIle: 4.687 ± 0.104
5.711GluLys: 5.711 ± 0.122
5.547GluLeu: 5.547 ± 0.129
1.685GluMet: 1.685 ± 0.054
4.065GluAsn: 4.065 ± 0.091
1.622GluPro: 1.622 ± 0.062
2.134GluGln: 2.134 ± 0.063
1.915GluArg: 1.915 ± 0.066
2.801GluSer: 2.801 ± 0.066
2.877GluThr: 2.877 ± 0.087
4.204GluVal: 4.204 ± 0.103
0.549GluTrp: 0.549 ± 0.031
2.005GluTyr: 2.005 ± 0.06
0.0GluXaa: 0.0 ± 0.0
Phe
2.929PheAla: 2.929 ± 0.078
0.279PheCys: 0.279 ± 0.024
2.783PheAsp: 2.783 ± 0.077
2.222PheGlu: 2.222 ± 0.069
1.957PhePhe: 1.957 ± 0.078
2.906PheGly: 2.906 ± 0.091
0.738PheHis: 0.738 ± 0.044
3.405PheIle: 3.405 ± 0.102
3.095PheLys: 3.095 ± 0.089
3.813PheLeu: 3.813 ± 0.126
0.992PheMet: 0.992 ± 0.046
2.612PheAsn: 2.612 ± 0.081
1.317PhePro: 1.317 ± 0.047
1.126PheGln: 1.126 ± 0.052
1.166PheArg: 1.166 ± 0.052
2.949PheSer: 2.949 ± 0.08
2.579PheThr: 2.579 ± 0.081
2.892PheVal: 2.892 ± 0.077
0.534PheTrp: 0.534 ± 0.036
1.716PheTyr: 1.716 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
4.295GlyAla: 4.295 ± 0.112
0.333GlyCys: 0.333 ± 0.026
3.43GlyAsp: 3.43 ± 0.088
3.527GlyGlu: 3.527 ± 0.089
2.77GlyPhe: 2.77 ± 0.083
3.867GlyGly: 3.867 ± 0.107
1.29GlyHis: 1.29 ± 0.055
5.607GlyIle: 5.607 ± 0.13
5.809GlyLys: 5.809 ± 0.143
5.733GlyLeu: 5.733 ± 0.141
1.836GlyMet: 1.836 ± 0.064
3.396GlyAsn: 3.396 ± 0.105
1.438GlyPro: 1.438 ± 0.052
2.544GlyGln: 2.544 ± 0.088
2.294GlyArg: 2.294 ± 0.078
3.975GlySer: 3.975 ± 0.091
3.914GlyThr: 3.914 ± 0.127
4.297GlyVal: 4.297 ± 0.086
0.715GlyTrp: 0.715 ± 0.041
3.068GlyTyr: 3.068 ± 0.11
0.0GlyXaa: 0.0 ± 0.0
His
1.204HisAla: 1.204 ± 0.045
0.111HisCys: 0.111 ± 0.014
1.244HisAsp: 1.244 ± 0.051
1.104HisGlu: 1.104 ± 0.043
0.973HisPhe: 0.973 ± 0.041
1.459HisGly: 1.459 ± 0.055
0.549HisHis: 0.549 ± 0.029
1.403HisIle: 1.403 ± 0.044
1.274HisLys: 1.274 ± 0.049
1.697HisLeu: 1.697 ± 0.058
0.461HisMet: 0.461 ± 0.025
1.015HisAsn: 1.015 ± 0.04
0.942HisPro: 0.942 ± 0.045
0.735HisGln: 0.735 ± 0.038
0.741HisArg: 0.741 ± 0.038
1.018HisSer: 1.018 ± 0.044
1.0HisThr: 1.0 ± 0.04
1.245HisVal: 1.245 ± 0.048
0.199HisTrp: 0.199 ± 0.02
0.969HisTyr: 0.969 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
5.73IleAla: 5.73 ± 0.126
0.541IleCys: 0.541 ± 0.034
4.806IleAsp: 4.806 ± 0.105
3.995IleGlu: 3.995 ± 0.094
3.582IlePhe: 3.582 ± 0.107
5.186IleGly: 5.186 ± 0.122
1.333IleHis: 1.333 ± 0.054
6.345IleIle: 6.345 ± 0.157
6.083IleLys: 6.083 ± 0.121
6.919IleLeu: 6.919 ± 0.178
1.763IleMet: 1.763 ± 0.07
4.919IleAsn: 4.919 ± 0.114
3.362IlePro: 3.362 ± 0.078
2.451IleGln: 2.451 ± 0.077
2.408IleArg: 2.408 ± 0.073
5.156IleSer: 5.156 ± 0.095
4.791IleThr: 4.791 ± 0.136
5.443IleVal: 5.443 ± 0.111
0.735IleTrp: 0.735 ± 0.045
2.794IleTyr: 2.794 ± 0.083
0.0IleXaa: 0.0 ± 0.0
Lys
5.126LysAla: 5.126 ± 0.12
0.237LysCys: 0.237 ± 0.022
5.247LysAsp: 5.247 ± 0.164
5.343LysGlu: 5.343 ± 0.118
3.073LysPhe: 3.073 ± 0.074
4.05LysGly: 4.05 ± 0.1
1.532LysHis: 1.532 ± 0.047
6.466LysIle: 6.466 ± 0.117
8.294LysLys: 8.294 ± 0.211
7.116LysLeu: 7.116 ± 0.142
2.652LysMet: 2.652 ± 0.084
6.007LysAsn: 6.007 ± 0.129
2.595LysPro: 2.595 ± 0.081
3.416LysGln: 3.416 ± 0.073
3.189LysArg: 3.189 ± 0.096
4.529LysSer: 4.529 ± 0.101
4.967LysThr: 4.967 ± 0.133
5.345LysVal: 5.345 ± 0.106
0.756LysTrp: 0.756 ± 0.035
3.551LysTyr: 3.551 ± 0.086
0.0LysXaa: 0.0 ± 0.0
Leu
6.997LeuAla: 6.997 ± 0.147
0.502LeuCys: 0.502 ± 0.029
5.186LeuAsp: 5.186 ± 0.11
4.637LeuGlu: 4.637 ± 0.102
3.711LeuPhe: 3.711 ± 0.114
5.731LeuGly: 5.731 ± 0.134
1.682LeuHis: 1.682 ± 0.057
7.113LeuIle: 7.113 ± 0.184
7.043LeuLys: 7.043 ± 0.139
7.77LeuLeu: 7.77 ± 0.188
2.214LeuMet: 2.214 ± 0.071
5.453LeuAsn: 5.453 ± 0.128
3.486LeuPro: 3.486 ± 0.091
3.017LeuGln: 3.017 ± 0.088
3.061LeuArg: 3.061 ± 0.09
5.974LeuSer: 5.974 ± 0.134
5.854LeuThr: 5.854 ± 0.092
5.947LeuVal: 5.947 ± 0.124
0.824LeuTrp: 0.824 ± 0.041
2.74LeuTyr: 2.74 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
1.993MetAla: 1.993 ± 0.068
0.123MetCys: 0.123 ± 0.015
1.373MetAsp: 1.373 ± 0.053
1.262MetGlu: 1.262 ± 0.048
0.932MetPhe: 0.932 ± 0.044
1.564MetGly: 1.564 ± 0.054
0.494MetHis: 0.494 ± 0.029
2.045MetIle: 2.045 ± 0.072
2.131MetLys: 2.131 ± 0.073
2.144MetLeu: 2.144 ± 0.072
0.826MetMet: 0.826 ± 0.044
1.584MetAsn: 1.584 ± 0.059
1.181MetPro: 1.181 ± 0.046
1.033MetGln: 1.033 ± 0.044
0.847MetArg: 0.847 ± 0.04
1.566MetSer: 1.566 ± 0.052
1.665MetThr: 1.665 ± 0.057
1.66MetVal: 1.66 ± 0.056
0.214MetTrp: 0.214 ± 0.022
0.746MetTyr: 0.746 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.705AsnAla: 3.705 ± 0.094
0.323AsnCys: 0.323 ± 0.03
3.265AsnAsp: 3.265 ± 0.105
3.285AsnGlu: 3.285 ± 0.087
2.673AsnPhe: 2.673 ± 0.071
4.358AsnGly: 4.358 ± 0.113
1.272AsnHis: 1.272 ± 0.049
4.337AsnIle: 4.337 ± 0.106
5.186AsnLys: 5.186 ± 0.131
5.309AsnLeu: 5.309 ± 0.125
1.453AsnMet: 1.453 ± 0.057
4.161AsnAsn: 4.161 ± 0.11
2.814AsnPro: 2.814 ± 0.136
2.566AsnGln: 2.566 ± 0.081
1.945AsnArg: 1.945 ± 0.072
3.794AsnSer: 3.794 ± 0.093
3.212AsnThr: 3.212 ± 0.103
3.642AsnVal: 3.642 ± 0.089
0.844AsnTrp: 0.844 ± 0.042
2.831AsnTyr: 2.831 ± 0.085
0.0AsnXaa: 0.0 ± 0.0
Pro
2.41ProAla: 2.41 ± 0.069
0.09ProCys: 0.09 ± 0.012
2.702ProAsp: 2.702 ± 0.143
2.882ProGlu: 2.882 ± 0.089
1.521ProPhe: 1.521 ± 0.065
2.202ProGly: 2.202 ± 0.113
0.673ProHis: 0.673 ± 0.036
2.645ProIle: 2.645 ± 0.071
2.798ProLys: 2.798 ± 0.083
2.748ProLeu: 2.748 ± 0.077
0.746ProMet: 0.746 ± 0.036
2.4ProAsn: 2.4 ± 0.115
0.469ProPro: 0.469 ± 0.032
1.4ProGln: 1.4 ± 0.058
0.955ProArg: 0.955 ± 0.045
2.166ProSer: 2.166 ± 0.075
2.531ProThr: 2.531 ± 0.126
2.897ProVal: 2.897 ± 0.13
0.33ProTrp: 0.33 ± 0.024
1.408ProTyr: 1.408 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
2.65GlnAla: 2.65 ± 0.074
0.096GlnCys: 0.096 ± 0.013
2.227GlnAsp: 2.227 ± 0.062
2.519GlnGlu: 2.519 ± 0.073
1.305GlnPhe: 1.305 ± 0.051
1.98GlnGly: 1.98 ± 0.066
0.589GlnHis: 0.589 ± 0.029
3.046GlnIle: 3.046 ± 0.082
3.645GlnLys: 3.645 ± 0.083
3.476GlnLeu: 3.476 ± 0.091
1.071GlnMet: 1.071 ± 0.051
2.473GlnAsn: 2.473 ± 0.072
1.05GlnPro: 1.05 ± 0.051
1.317GlnGln: 1.317 ± 0.056
1.418GlnArg: 1.418 ± 0.06
1.945GlnSer: 1.945 ± 0.066
2.426GlnThr: 2.426 ± 0.122
2.645GlnVal: 2.645 ± 0.077
0.295GlnTrp: 0.295 ± 0.022
1.386GlnTyr: 1.386 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
2.108ArgAla: 2.108 ± 0.073
0.134ArgCys: 0.134 ± 0.014
1.969ArgAsp: 1.969 ± 0.06
2.159ArgGlu: 2.159 ± 0.069
1.436ArgPhe: 1.436 ± 0.058
1.945ArgGly: 1.945 ± 0.07
0.793ArgHis: 0.793 ± 0.042
2.655ArgIle: 2.655 ± 0.078
2.965ArgLys: 2.965 ± 0.092
3.118ArgLeu: 3.118 ± 0.09
0.965ArgMet: 0.965 ± 0.044
1.821ArgAsn: 1.821 ± 0.059
1.151ArgPro: 1.151 ± 0.052
1.362ArgGln: 1.362 ± 0.053
1.711ArgArg: 1.711 ± 0.072
1.77ArgSer: 1.77 ± 0.051
1.76ArgThr: 1.76 ± 0.054
2.36ArgVal: 2.36 ± 0.078
0.277ArgTrp: 0.277 ± 0.024
1.444ArgTyr: 1.444 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
4.002SerAla: 4.002 ± 0.079
0.255SerCys: 0.255 ± 0.02
3.796SerAsp: 3.796 ± 0.1
3.37SerGlu: 3.37 ± 0.092
2.851SerPhe: 2.851 ± 0.076
4.426SerGly: 4.426 ± 0.122
1.184SerHis: 1.184 ± 0.053
4.435SerIle: 4.435 ± 0.096
4.808SerLys: 4.808 ± 0.109
5.61SerLeu: 5.61 ± 0.114
1.403SerMet: 1.403 ± 0.05
3.536SerAsn: 3.536 ± 0.097
1.813SerPro: 1.813 ± 0.068
2.634SerGln: 2.634 ± 0.083
2.136SerArg: 2.136 ± 0.067
4.37SerSer: 4.37 ± 0.117
3.491SerThr: 3.491 ± 0.098
3.909SerVal: 3.909 ± 0.082
0.736SerTrp: 0.736 ± 0.038
2.622SerTyr: 2.622 ± 0.08
0.0SerXaa: 0.0 ± 0.0
Thr
4.201ThrAla: 4.201 ± 0.104
0.257ThrCys: 0.257 ± 0.023
3.411ThrAsp: 3.411 ± 0.149
2.952ThrGlu: 2.952 ± 0.077
2.423ThrPhe: 2.423 ± 0.081
4.345ThrGly: 4.345 ± 0.146
1.088ThrHis: 1.088 ± 0.046
5.083ThrIle: 5.083 ± 0.162
4.571ThrLys: 4.571 ± 0.122
5.022ThrLeu: 5.022 ± 0.084
1.232ThrMet: 1.232 ± 0.043
3.315ThrAsn: 3.315 ± 0.099
3.217ThrPro: 3.217 ± 0.225
2.187ThrGln: 2.187 ± 0.071
1.922ThrArg: 1.922 ± 0.067
3.786ThrSer: 3.786 ± 0.103
3.983ThrThr: 3.983 ± 0.18
4.357ThrVal: 4.357 ± 0.211
0.647ThrTrp: 0.647 ± 0.038
2.38ThrTyr: 2.38 ± 0.113
0.0ThrXaa: 0.0 ± 0.0
Val
5.012ValAla: 5.012 ± 0.119
0.343ValCys: 0.343 ± 0.025
4.315ValAsp: 4.315 ± 0.15
3.607ValGlu: 3.607 ± 0.092
2.549ValPhe: 2.549 ± 0.071
4.393ValGly: 4.393 ± 0.105
1.26ValHis: 1.26 ± 0.06
5.317ValIle: 5.317 ± 0.1
5.39ValLys: 5.39 ± 0.115
5.839ValLeu: 5.839 ± 0.126
1.657ValMet: 1.657 ± 0.067
3.846ValAsn: 3.846 ± 0.114
2.662ValPro: 2.662 ± 0.11
2.302ValGln: 2.302 ± 0.07
2.093ValArg: 2.093 ± 0.074
4.325ValSer: 4.325 ± 0.09
4.713ValThr: 4.713 ± 0.248
4.972ValVal: 4.972 ± 0.127
0.599ValTrp: 0.599 ± 0.037
2.287ValTyr: 2.287 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.595TrpAla: 0.595 ± 0.03
0.06TrpCys: 0.06 ± 0.01
0.549TrpAsp: 0.549 ± 0.03
0.398TrpGlu: 0.398 ± 0.027
0.494TrpPhe: 0.494 ± 0.041
0.675TrpGly: 0.675 ± 0.039
0.247TrpHis: 0.247 ± 0.019
0.826TrpIle: 0.826 ± 0.04
0.703TrpLys: 0.703 ± 0.044
1.149TrpLeu: 1.149 ± 0.054
0.307TrpMet: 0.307 ± 0.024
0.688TrpAsn: 0.688 ± 0.041
0.303TrpPro: 0.303 ± 0.022
0.554TrpGln: 0.554 ± 0.03
0.345TrpArg: 0.345 ± 0.025
0.599TrpSer: 0.599 ± 0.036
0.605TrpThr: 0.605 ± 0.05
0.634TrpVal: 0.634 ± 0.039
0.139TrpTrp: 0.139 ± 0.013
0.42TrpTyr: 0.42 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.343TyrAla: 2.343 ± 0.073
0.222TyrCys: 0.222 ± 0.018
2.398TyrAsp: 2.398 ± 0.068
2.051TyrGlu: 2.051 ± 0.061
1.932TyrPhe: 1.932 ± 0.065
2.6TyrGly: 2.6 ± 0.068
1.01TyrHis: 1.01 ± 0.044
2.639TyrIle: 2.639 ± 0.068
2.693TyrLys: 2.693 ± 0.071
3.882TyrLeu: 3.882 ± 0.108
0.794TyrMet: 0.794 ± 0.044
2.307TyrAsn: 2.307 ± 0.065
1.521TyrPro: 1.521 ± 0.055
1.731TyrGln: 1.731 ± 0.062
1.549TyrArg: 1.549 ± 0.055
2.448TyrSer: 2.448 ± 0.067
2.449TyrThr: 2.449 ± 0.128
2.564TyrVal: 2.564 ± 0.078
0.476TyrTrp: 0.476 ± 0.029
1.866TyrTyr: 1.866 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1826 proteins (602995 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski