Podoviridae sp. ctka020
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W699|A0A5Q2W699_9CAUD Uncharacterized protein OS=Podoviridae sp. ctka020 OX=2656713 PE=4 SV=1
MM1 pKa = 6.92 GAEE4 pKa = 4.1 RR5 pKa = 11.84 EE6 pKa = 4.18 RR7 pKa = 11.84 PEE9 pKa = 4.56 IMTTEE14 pKa = 4.16 DD15 pKa = 4.3 FINDD19 pKa = 3.23 VDD21 pKa = 4.04 PATEE25 pKa = 4.0 AKK27 pKa = 10.06 QDD29 pKa = 3.66 VGNTTLSTISAKK41 pKa = 10.58 LITGIPLVPTTSGGLTIYY59 pKa = 10.77 RR60 pKa = 11.84 NIDD63 pKa = 3.41 LDD65 pKa = 3.8 EE66 pKa = 4.46 TGQNIKK72 pKa = 10.79 GSTGQLYY79 pKa = 10.54 GYY81 pKa = 9.97 YY82 pKa = 9.72 FYY84 pKa = 11.34 NAAATVRR91 pKa = 11.84 YY92 pKa = 9.86 LKK94 pKa = 10.44 FYY96 pKa = 11.18 NKK98 pKa = 8.59 ATAPTVGSDD107 pKa = 3.72 TPVLTLPLPPGAAGHH122 pKa = 6.09 VEE124 pKa = 4.31 FTDD127 pKa = 4.47 GVAFSTGIGAGATTGVADD145 pKa = 4.67 NDD147 pKa = 3.96 TGAPSANDD155 pKa = 3.09 VVVNILYY162 pKa = 10.23 KK163 pKa = 10.77
Molecular weight: 17.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.333
IPC2_protein 4.38
IPC_protein 4.291
Toseland 4.101
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.19
Rodwell 4.126
Grimsley 4.012
Solomon 4.266
Lehninger 4.215
Nozaki 4.38
DTASelect 4.596
Thurlkill 4.139
EMBOSS 4.202
Sillero 4.406
Patrickios 3.478
IPC_peptide 4.266
IPC2_peptide 4.393
IPC2.peptide.svr19 4.334
Protein with the highest isoelectric point:
>tr|A0A5Q2W6B3|A0A5Q2W6B3_9CAUD Uncharacterized protein OS=Podoviridae sp. ctka020 OX=2656713 PE=4 SV=1
MM1 pKa = 6.88 QRR3 pKa = 11.84 RR4 pKa = 11.84 EE5 pKa = 3.89 RR6 pKa = 11.84 THH8 pKa = 7.46 RR9 pKa = 11.84 LILLPYY15 pKa = 9.56 RR16 pKa = 11.84 GFKK19 pKa = 9.83 EE20 pKa = 4.17 FCEE23 pKa = 4.78 FPFVSCVRR31 pKa = 11.84 LFPLRR36 pKa = 11.84 EE37 pKa = 3.75 PVLNGIAA44 pKa = 3.59
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.209
IPC_protein 9.999
Toseland 10.014
ProMoST 9.853
Dawson 10.218
Bjellqvist 10.072
Wikipedia 10.452
Rodwell 10.248
Grimsley 10.292
Solomon 10.394
Lehninger 10.365
Nozaki 10.218
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.423
Sillero 10.218
Patrickios 10.292
IPC_peptide 10.379
IPC2_peptide 9.633
IPC2.peptide.svr19 8.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10245
30
920
232.8
25.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.15 ± 0.569
0.996 ± 0.19
5.359 ± 0.237
6.257 ± 0.566
3.885 ± 0.276
7.301 ± 0.537
1.571 ± 0.189
6.218 ± 0.22
7.477 ± 0.722
8.023 ± 0.332
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.304 ± 0.175
4.998 ± 0.265
4.158 ± 0.328
4.08 ± 0.317
4.461 ± 0.34
6.657 ± 0.517
7.418 ± 0.589
5.993 ± 0.291
1.24 ± 0.187
3.455 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here