Gloeocapsa sp. PCC 73106
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4062 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L8LR29|L8LR29_9CHRO Uncharacterized protein OS=Gloeocapsa sp. PCC 73106 OX=102232 GN=GLO73106DRAFT_00014570 PE=4 SV=1
GG1 pKa = 7.54 DD2 pKa = 3.73 APAPVVGLINQYY14 pKa = 9.78 GNRR17 pKa = 11.84 GQNIPWNIAIANDD30 pKa = 3.76 DD31 pKa = 3.92 SGDD34 pKa = 3.6 VYY36 pKa = 11.25 ATGTTAGNVGGPSAGSLDD54 pKa = 3.97 GYY56 pKa = 10.74 LLQFNQIGVRR66 pKa = 11.84 GWSKK70 pKa = 10.79 QLGTTEE76 pKa = 3.64 IDD78 pKa = 3.13 NIYY81 pKa = 10.55 GVNTNTDD88 pKa = 2.64 GDD90 pKa = 4.41 VYY92 pKa = 10.87 FVGRR96 pKa = 11.84 TKK98 pKa = 11.06 GSLVAPNAGIGNDD111 pKa = 3.24 IVFGKK116 pKa = 10.51 YY117 pKa = 10.22 DD118 pKa = 3.58 SDD120 pKa = 5.5 GNEE123 pKa = 3.37 QWIQQFGSFGVDD135 pKa = 2.79 NSFVNPEE142 pKa = 4.05 LDD144 pKa = 2.95 ASGNVYY150 pKa = 10.55 VGGYY154 pKa = 8.57 TDD156 pKa = 4.62 GSLFGPNAGAAFPPSLDD173 pKa = 2.98 PWVAKK178 pKa = 10.29 FDD180 pKa = 4.37 PDD182 pKa = 5.29 GNQLWARR189 pKa = 11.84 QFGSPTGDD197 pKa = 3.05 EE198 pKa = 4.63 LFGLDD203 pKa = 4.12 VDD205 pKa = 4.23 SQGNVLATGWTFGGLAGPNNVNVDD229 pKa = 3.34 GAATYY234 pKa = 9.65 DD235 pKa = 2.82 IWLQKK240 pKa = 9.91 MDD242 pKa = 4.11 TNGNTLWTEE251 pKa = 3.91 QFGTNVDD258 pKa = 3.06 DD259 pKa = 4.13 WAWDD263 pKa = 3.33 IEE265 pKa = 4.44 TNAAGEE271 pKa = 4.27 IYY273 pKa = 9.74 LTGWTLGNLGGTNAGSYY290 pKa = 10.4 DD291 pKa = 4.07 YY292 pKa = 11.16 YY293 pKa = 9.47 IAKK296 pKa = 10.01 YY297 pKa = 10.26 SSSGDD302 pKa = 3.69 QLWVRR307 pKa = 11.84 QYY309 pKa = 10.46 GTTGDD314 pKa = 3.92 DD315 pKa = 3.2 AATRR319 pKa = 11.84 MALDD323 pKa = 4.15 DD324 pKa = 4.42 NGNIYY329 pKa = 8.91 LTGYY333 pKa = 10.69 SNGPASGQVFGTSGGYY349 pKa = 9.3 DD350 pKa = 2.7 AWVAKK355 pKa = 10.1 FDD357 pKa = 4.64 SEE359 pKa = 5.21 GNSIWRR365 pKa = 11.84 KK366 pKa = 7.58 TIGTVEE372 pKa = 4.28 LDD374 pKa = 3.35 QAFGIAVQGNDD385 pKa = 3.92 LLVSGLTEE393 pKa = 4.31 GSLGAVNQGSYY404 pKa = 10.77 DD405 pKa = 3.15 GWIARR410 pKa = 11.84 LTTTTGNLLSFNGLGG425 pKa = 3.38
Molecular weight: 44.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.643
IPC_protein 3.681
Toseland 3.439
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.668
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.808
Patrickios 0.922
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|L8LMR8|L8LMR8_9CHRO Transketolase OS=Gloeocapsa sp. PCC 73106 OX=102232 GN=GLO73106DRAFT_00026810 PE=4 SV=1
MM1 pKa = 7.36 ATNDD5 pKa = 4.48 TISDD9 pKa = 3.51 MLTRR13 pKa = 11.84 IRR15 pKa = 11.84 NACLVRR21 pKa = 11.84 QSSTHH26 pKa = 5.07 VPTTRR31 pKa = 11.84 MTRR34 pKa = 11.84 NIARR38 pKa = 11.84 VLKK41 pKa = 10.35 EE42 pKa = 3.62 EE43 pKa = 4.29 GFIEE47 pKa = 4.29 DD48 pKa = 3.71 FQEE51 pKa = 4.2 IGEE54 pKa = 4.51 GIKK57 pKa = 10.32 RR58 pKa = 11.84 QLVISLRR65 pKa = 11.84 YY66 pKa = 8.71 KK67 pKa = 10.69 GKK69 pKa = 10.14 AGRR72 pKa = 11.84 PIIRR76 pKa = 11.84 TLKK79 pKa = 10.06 RR80 pKa = 11.84 VSKK83 pKa = 10.24 PGLRR87 pKa = 11.84 VYY89 pKa = 9.37 TNRR92 pKa = 11.84 KK93 pKa = 7.66 EE94 pKa = 4.01 MPRR97 pKa = 11.84 VLGGIGIAIISTSQGIMTDD116 pKa = 3.34 RR117 pKa = 11.84 EE118 pKa = 4.08 ARR120 pKa = 11.84 RR121 pKa = 11.84 QGIGGEE127 pKa = 3.97 ILCYY131 pKa = 10.3 VWW133 pKa = 4.93
Molecular weight: 15.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.692
IPC_protein 10.657
Toseland 10.862
ProMoST 10.657
Dawson 10.921
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.052
Grimsley 10.965
Solomon 11.111
Lehninger 11.067
Nozaki 10.847
DTASelect 10.672
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.862
Patrickios 10.789
IPC_peptide 11.125
IPC2_peptide 9.809
IPC2.peptide.svr19 8.639
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4062
0
4062
1199467
20
2695
295.3
32.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.43 ± 0.042
0.927 ± 0.013
4.878 ± 0.035
6.542 ± 0.038
3.98 ± 0.028
6.877 ± 0.059
1.723 ± 0.019
7.38 ± 0.032
4.659 ± 0.042
11.438 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.736 ± 0.015
4.523 ± 0.036
4.604 ± 0.03
5.199 ± 0.038
4.858 ± 0.029
6.52 ± 0.032
5.773 ± 0.029
6.331 ± 0.032
1.415 ± 0.021
3.208 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here