Leersia perrieri

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Leersia

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D9XD02|A0A0D9XD02_9ORYZ NB-ARC domain-containing protein OS=Leersia perrieri OX=77586 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 5.24AAANDD7 pKa = 3.68STAADD12 pKa = 3.9QNPTTTPNPNPNPPSAAAAGPDD34 pKa = 3.77DD35 pKa = 4.05SAAAATRR42 pKa = 11.84RR43 pKa = 11.84PFTSLTQEE51 pKa = 4.12EE52 pKa = 4.68ADD54 pKa = 3.55LALARR59 pKa = 11.84VLQEE63 pKa = 3.57QEE65 pKa = 3.57RR66 pKa = 11.84AYY68 pKa = 10.61MMLSGGHH75 pKa = 6.19HH76 pKa = 6.53GGGGEE81 pKa = 4.13YY82 pKa = 10.31AASDD86 pKa = 3.67DD87 pKa = 3.89GGSYY91 pKa = 10.44EE92 pKa = 5.55FDD94 pKa = 3.69YY95 pKa = 11.46DD96 pKa = 5.3DD97 pKa = 6.16DD98 pKa = 7.15DD99 pKa = 6.97GDD101 pKa = 3.93GLEE104 pKa = 4.53GEE106 pKa = 4.69EE107 pKa = 4.43GSDD110 pKa = 3.7YY111 pKa = 11.5DD112 pKa = 4.69EE113 pKa = 5.24EE114 pKa = 6.82DD115 pKa = 3.75GDD117 pKa = 4.29GHH119 pKa = 7.69GEE121 pKa = 3.92APLDD125 pKa = 3.65EE126 pKa = 5.74DD127 pKa = 4.45EE128 pKa = 5.01EE129 pKa = 4.49VGEE132 pKa = 4.63ADD134 pKa = 5.05DD135 pKa = 5.06GGAAAAAAGLDD146 pKa = 3.51PAQYY150 pKa = 11.24DD151 pKa = 3.55DD152 pKa = 5.63DD153 pKa = 4.26EE154 pKa = 6.03AFARR158 pKa = 11.84ALQDD162 pKa = 3.22AEE164 pKa = 4.05EE165 pKa = 4.96RR166 pKa = 11.84EE167 pKa = 4.33VAGRR171 pKa = 11.84LMALAGLTGYY181 pKa = 10.45VDD183 pKa = 3.81PDD185 pKa = 3.4EE186 pKa = 4.53YY187 pKa = 11.33SYY189 pKa = 11.93EE190 pKa = 4.0EE191 pKa = 4.28LVALGEE197 pKa = 4.47VVGTEE202 pKa = 4.31SRR204 pKa = 11.84GLPADD209 pKa = 3.85TLASLPSVTYY219 pKa = 10.46RR220 pKa = 11.84AQDD223 pKa = 3.38KK224 pKa = 10.88QDD226 pKa = 3.6GNMEE230 pKa = 3.85QCVICRR236 pKa = 11.84VEE238 pKa = 4.1FEE240 pKa = 4.31EE241 pKa = 5.51GEE243 pKa = 4.47SLVALPCKK251 pKa = 10.13HH252 pKa = 6.85SYY254 pKa = 10.7HH255 pKa = 7.0SDD257 pKa = 4.29CINQWLQLNKK267 pKa = 9.62VCPMCSAEE275 pKa = 4.16VSTSRR280 pKa = 11.84DD281 pKa = 2.88NRR283 pKa = 11.84AA284 pKa = 2.93

Molecular weight: 30.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D9WDL8|A0A0D9WDL8_9ORYZ Uncharacterized protein OS=Leersia perrieri OX=77586 PE=4 SV=1
MM1 pKa = 7.56GFGSGITGAPRR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84AGRR22 pKa = 11.84TPRR25 pKa = 11.84VLLSARR31 pKa = 3.72

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29035

7744

36779

16268560

7

5221

442.3

48.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.771 ± 0.017

1.9 ± 0.007

5.484 ± 0.008

6.068 ± 0.014

3.723 ± 0.008

7.165 ± 0.013

2.486 ± 0.006

4.706 ± 0.009

5.063 ± 0.012

9.556 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.005

3.736 ± 0.009

5.373 ± 0.013

3.436 ± 0.01

6.109 ± 0.009

8.395 ± 0.014

4.882 ± 0.007

6.758 ± 0.009

1.285 ± 0.005

2.629 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski