Actinobacteria bacterium IMCC26207

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 5.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2975 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J0UXG5|A0A0J0UXG5_9ACTN Methylmalonyl-CoA epimerase OS=Actinobacteria bacterium IMCC26207 OX=1641811 GN=IMCC26207_10743 PE=3 SV=1
MM1 pKa = 7.26KK2 pKa = 10.13NAYY5 pKa = 9.15RR6 pKa = 11.84VLPVVSAALLLFAVSCAPAPGGGGTANLAPIAVATASPLSGNAPLSAAFDD56 pKa = 4.03GSGSVDD62 pKa = 3.08SDD64 pKa = 3.67GSIVSYY70 pKa = 10.4SWSFGNGTNGSGVTSTGNYY89 pKa = 5.27PTAGAFTAVLTVTDD103 pKa = 4.2NKK105 pKa = 10.94GATDD109 pKa = 3.85TDD111 pKa = 4.02SLTITVNGDD120 pKa = 2.91GDD122 pKa = 4.14GDD124 pKa = 4.4GVFVPSDD131 pKa = 3.71CNDD134 pKa = 3.23ADD136 pKa = 3.92GAIFPGAADD145 pKa = 3.64PAGDD149 pKa = 4.78NIDD152 pKa = 4.34SNCDD156 pKa = 3.59GIDD159 pKa = 3.63GTEE162 pKa = 3.94SAAVFVNSSTGANTSTCGTILEE184 pKa = 4.52PCASITQGQSRR195 pKa = 11.84AVAEE199 pKa = 4.55SKK201 pKa = 10.93SSVFVAGGSYY211 pKa = 11.02SKK213 pKa = 10.18FTVQAGLEE221 pKa = 4.06VRR223 pKa = 11.84GGYY226 pKa = 8.54GQNFQRR232 pKa = 11.84GVLATSPSIATVTAAFDD249 pKa = 3.5ATVNGPVGVIADD261 pKa = 5.29GISTATRR268 pKa = 11.84VADD271 pKa = 3.92LSIVGTTASAGSSSYY286 pKa = 11.38GVFVRR291 pKa = 11.84NSTNALVLDD300 pKa = 4.18SLNIAGGVAGAGSAGAAGSSGWSGTAGTGSNGGDD334 pKa = 3.2GFEE337 pKa = 5.28PGGACNTSSAGGGGAGGGGANSGGSGGKK365 pKa = 10.24GGVVDD370 pKa = 6.53GSCGWTGLCSNCDD383 pKa = 3.42AQTGNSGGSGGGVGGGGGGAAGSAATDD410 pKa = 3.79GLVGICTLGGTGPTLRR426 pKa = 11.84GGSGSSGANGTAGGAGSGGSAGANGATGLLGANGAGGGAGGGGGANDD473 pKa = 4.97CNIDD477 pKa = 3.63DD478 pKa = 4.77AGAGGGGGGGGGTRR492 pKa = 11.84ATAGGAGGGAGAQSIGLRR510 pKa = 11.84LQNASPTLIAIQIQLGTGGAGGTGGSGAAGQPGGSGGSGGVAFDD554 pKa = 3.9RR555 pKa = 11.84GGGGGNGGTGGTGGASGAGGGGGGGAAIGLSRR587 pKa = 11.84DD588 pKa = 3.52GSSTPSGSPSYY599 pKa = 10.73SGGVGGSGGSAGSGGNPGATGLVTNTTVAA628 pKa = 3.31

Molecular weight: 55.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J0V049|A0A0J0V049_9ACTN Homoserine dehydrogenase OS=Actinobacteria bacterium IMCC26207 OX=1641811 GN=IMCC26207_10385 PE=3 SV=1
MM1 pKa = 7.45QPAQRR6 pKa = 11.84LASSKK11 pKa = 10.58SLIWLRR17 pKa = 11.84LVGLAPGRR25 pKa = 11.84VSRR28 pKa = 11.84AAILLGLGAFIAAVYY43 pKa = 10.27LPGSVVPDD51 pKa = 3.72TLDD54 pKa = 2.98MCGQAVSGVYY64 pKa = 10.35NDD66 pKa = 3.22WHH68 pKa = 6.85SPAIAGLWGFFNPRR82 pKa = 11.84IEE84 pKa = 4.7VIFLTTLAATILAVHH99 pKa = 7.19LILTRR104 pKa = 11.84WLRR107 pKa = 11.84PWVAVACTAAIMLFPATVGWMGHH130 pKa = 4.62VGKK133 pKa = 10.44DD134 pKa = 2.99EE135 pKa = 3.91WFAAAFLLGTALLARR150 pKa = 11.84AGTEE154 pKa = 3.33RR155 pKa = 11.84RR156 pKa = 11.84VRR158 pKa = 11.84LRR160 pKa = 11.84RR161 pKa = 11.84ALLMGAMICFWFAIAARR178 pKa = 11.84KK179 pKa = 8.68NALLPVGAALLVAWPVPGSVFGRR202 pKa = 11.84LQDD205 pKa = 3.34RR206 pKa = 11.84LLVRR210 pKa = 11.84RR211 pKa = 11.84VLAAGGVLILLVGSVSLFTSAIVRR235 pKa = 11.84PRR237 pKa = 11.84PRR239 pKa = 11.84HH240 pKa = 5.67PEE242 pKa = 3.55QQAFLFDD249 pKa = 3.81LTGLALAQQQMLFPPGVFPDD269 pKa = 3.65GTTLSGIGQSFDD281 pKa = 3.61PKK283 pKa = 10.05TGDD286 pKa = 3.21VFFFAPGTPANPFMLAEE303 pKa = 4.0QVSAVKK309 pKa = 10.08EE310 pKa = 3.97KK311 pKa = 10.16WLEE314 pKa = 3.83AVLAHH319 pKa = 7.01PDD321 pKa = 3.0SYY323 pKa = 11.65LRR325 pKa = 11.84TRR327 pKa = 11.84LSYY330 pKa = 10.52TSALLGIAGAHH341 pKa = 6.5PYY343 pKa = 9.69WSINDD348 pKa = 4.17PGSLPATWGSNCRR361 pKa = 11.84VPNRR365 pKa = 11.84TFPSLHH371 pKa = 5.71QRR373 pKa = 11.84VLDD376 pKa = 3.68LLLRR380 pKa = 11.84VEE382 pKa = 4.53RR383 pKa = 11.84GNLFRR388 pKa = 11.84GWTFLVILIAGSLLAGVRR406 pKa = 11.84KK407 pKa = 8.48VTEE410 pKa = 3.9ARR412 pKa = 11.84VLLLGGLFSFAGIAIAGISPTFRR435 pKa = 11.84YY436 pKa = 8.95SWFMAVCALIVAALALRR453 pKa = 11.84RR454 pKa = 11.84IPFAARR460 pKa = 11.84PDD462 pKa = 3.93PPGEE466 pKa = 4.11PLASVPDD473 pKa = 3.97SDD475 pKa = 5.48ASRR478 pKa = 11.84SDD480 pKa = 3.64PSPSSFSATMPTGEE494 pKa = 4.48PRR496 pKa = 11.84SRR498 pKa = 11.84WEE500 pKa = 3.78KK501 pKa = 10.85VLVQMGLIPDD511 pKa = 3.89SS512 pKa = 3.78

Molecular weight: 55.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2975

0

2975

1027166

40

2413

345.3

37.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.81 ± 0.046

0.93 ± 0.013

5.63 ± 0.032

6.141 ± 0.043

3.294 ± 0.029

8.563 ± 0.049

1.908 ± 0.023

4.573 ± 0.032

2.344 ± 0.029

10.433 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.021

2.543 ± 0.03

5.452 ± 0.031

3.716 ± 0.027

6.167 ± 0.041

6.909 ± 0.04

5.933 ± 0.051

8.205 ± 0.04

1.487 ± 0.02

1.912 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski