Amino acid dipepetide frequency for Actinobacteria bacterium IMCC26207

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.827AlaAla: 16.827 ± 0.183	1.083AlaCys: 1.083 ± 0.035	6.876AlaAsp: 6.876 ± 0.094	8.118AlaGlu: 8.118 ± 0.088	3.442AlaPhe: 3.442 ± 0.063	10.419AlaGly: 10.419 ± 0.127	2.0AlaHis: 2.0 ± 0.042	4.982AlaIle: 4.982 ± 0.085	2.931AlaLys: 2.931 ± 0.062	11.712AlaLeu: 11.712 ± 0.137	2.48AlaMet: 2.48 ± 0.052	2.973AlaAsn: 2.973 ± 0.077	5.481AlaPro: 5.481 ± 0.107	4.726AlaGln: 4.726 ± 0.076	6.261AlaArg: 6.261 ± 0.088	7.142AlaSer: 7.142 ± 0.093	7.035AlaThr: 7.035 ± 0.102	10.107AlaVal: 10.107 ± 0.108	1.51AlaTrp: 1.51 ± 0.041	1.996AlaTyr: 1.996 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.075CysAla: 1.075 ± 0.035	0.134CysCys: 0.134 ± 0.013	0.578CysAsp: 0.578 ± 0.024	0.564CysGlu: 0.564 ± 0.021	0.319CysPhe: 0.319 ± 0.02	0.929CysGly: 0.929 ± 0.033	0.206CysHis: 0.206 ± 0.015	0.435CysIle: 0.435 ± 0.019	0.197CysLys: 0.197 ± 0.015	0.767CysLeu: 0.767 ± 0.031	0.181CysMet: 0.181 ± 0.014	0.219CysAsn: 0.219 ± 0.016	0.497CysPro: 0.497 ± 0.023	0.22CysGln: 0.22 ± 0.015	0.492CysArg: 0.492 ± 0.023	0.777CysSer: 0.777 ± 0.03	0.609CysThr: 0.609 ± 0.026	0.763CysVal: 0.763 ± 0.027	0.149CysTrp: 0.149 ± 0.015	0.193CysTyr: 0.193 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.029AspAla: 6.029 ± 0.083	0.542AspCys: 0.542 ± 0.022	2.696AspAsp: 2.696 ± 0.053	3.588AspGlu: 3.588 ± 0.071	1.607AspPhe: 1.607 ± 0.043	5.002AspGly: 5.002 ± 0.081	1.228AspHis: 1.228 ± 0.036	2.04AspIle: 2.04 ± 0.047	1.067AspLys: 1.067 ± 0.03	7.105AspLeu: 7.105 ± 0.102	0.947AspMet: 0.947 ± 0.031	1.086AspAsn: 1.086 ± 0.04	4.496AspPro: 4.496 ± 0.072	2.44AspGln: 2.44 ± 0.047	3.919AspArg: 3.919 ± 0.067	3.949AspSer: 3.949 ± 0.069	2.64AspThr: 2.64 ± 0.054	3.877AspVal: 3.877 ± 0.072	0.958AspTrp: 0.958 ± 0.031	1.082AspTyr: 1.082 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.422GluAla: 6.422 ± 0.089	0.429GluCys: 0.429 ± 0.018	3.073GluAsp: 3.073 ± 0.068	3.78GluGlu: 3.78 ± 0.083	2.374GluPhe: 2.374 ± 0.052	4.205GluGly: 4.205 ± 0.065	1.353GluHis: 1.353 ± 0.038	3.117GluIle: 3.117 ± 0.065	1.368GluLys: 1.368 ± 0.042	7.896GluLeu: 7.896 ± 0.12	1.326GluMet: 1.326 ± 0.04	1.38GluAsn: 1.38 ± 0.037	3.107GluPro: 3.107 ± 0.058	3.335GluGln: 3.335 ± 0.068	4.448GluArg: 4.448 ± 0.079	3.914GluSer: 3.914 ± 0.066	2.818GluThr: 2.818 ± 0.054	5.01GluVal: 5.01 ± 0.078	0.853GluTrp: 0.853 ± 0.03	1.204GluTyr: 1.204 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.92PheAla: 3.92 ± 0.071	0.411PheCys: 0.411 ± 0.019	2.424PheAsp: 2.424 ± 0.058	2.192PheGlu: 2.192 ± 0.046	1.189PhePhe: 1.189 ± 0.037	3.609PheGly: 3.609 ± 0.062	0.614PheHis: 0.614 ± 0.022	1.25PheIle: 1.25 ± 0.037	0.628PheLys: 0.628 ± 0.028	3.085PheLeu: 3.085 ± 0.064	0.612PheMet: 0.612 ± 0.028	0.874PheAsn: 0.874 ± 0.032	1.565PhePro: 1.565 ± 0.038	0.839PheGln: 0.839 ± 0.031	1.794PheArg: 1.794 ± 0.043	2.22PheSer: 2.22 ± 0.05	1.97PheThr: 1.97 ± 0.045	2.613PheVal: 2.613 ± 0.053	0.484PheTrp: 0.484 ± 0.022	0.641PheTyr: 0.641 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	9.131GlyAla: 9.131 ± 0.131	0.951GlyCys: 0.951 ± 0.033	4.38GlyAsp: 4.38 ± 0.071	4.792GlyGlu: 4.792 ± 0.074	3.204GlyPhe: 3.204 ± 0.061	7.36GlyGly: 7.36 ± 0.17	1.674GlyHis: 1.674 ± 0.049	4.405GlyIle: 4.405 ± 0.066	2.294GlyLys: 2.294 ± 0.052	8.791GlyLeu: 8.791 ± 0.114	1.967GlyMet: 1.967 ± 0.046	2.024GlyAsn: 2.024 ± 0.051	3.936GlyPro: 3.936 ± 0.071	3.019GlyGln: 3.019 ± 0.049	5.276GlyArg: 5.276 ± 0.077	6.609GlySer: 6.609 ± 0.127	5.216GlyThr: 5.216 ± 0.096	7.151GlyVal: 7.151 ± 0.092	1.557GlyTrp: 1.557 ± 0.041	1.896GlyTyr: 1.896 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.882HisAla: 1.882 ± 0.047	0.212HisCys: 0.212 ± 0.015	0.998HisAsp: 0.998 ± 0.033	1.144HisGlu: 1.144 ± 0.036	0.62HisPhe: 0.62 ± 0.023	1.691HisGly: 1.691 ± 0.05	0.571HisHis: 0.571 ± 0.024	0.737HisIle: 0.737 ± 0.028	0.387HisLys: 0.387 ± 0.02	2.218HisLeu: 2.218 ± 0.043	0.316HisMet: 0.316 ± 0.016	0.44HisAsn: 0.44 ± 0.022	1.42HisPro: 1.42 ± 0.031	0.737HisGln: 0.737 ± 0.025	1.49HisArg: 1.49 ± 0.048	1.162HisSer: 1.162 ± 0.037	0.956HisThr: 0.956 ± 0.033	1.37HisVal: 1.37 ± 0.036	0.339HisTrp: 0.339 ± 0.019	0.386HisTyr: 0.386 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.917IleAla: 5.917 ± 0.09	0.5IleCys: 0.5 ± 0.022	3.254IleAsp: 3.254 ± 0.057	3.291IleGlu: 3.291 ± 0.062	1.293IlePhe: 1.293 ± 0.038	4.419IleGly: 4.419 ± 0.076	0.864IleHis: 0.864 ± 0.03	1.903IleIle: 1.903 ± 0.048	0.88IleLys: 0.88 ± 0.031	4.001IleLeu: 4.001 ± 0.073	0.722IleMet: 0.722 ± 0.026	1.233IleAsn: 1.233 ± 0.033	2.528IlePro: 2.528 ± 0.049	1.357IleGln: 1.357 ± 0.041	2.649IleArg: 2.649 ± 0.057	3.139IleSer: 3.139 ± 0.062	2.839IleThr: 2.839 ± 0.06	3.516IleVal: 3.516 ± 0.063	0.627IleTrp: 0.627 ± 0.027	0.801IleTyr: 0.801 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	2.581LysAla: 2.581 ± 0.062	0.129LysCys: 0.129 ± 0.011	1.146LysAsp: 1.146 ± 0.04	1.217LysGlu: 1.217 ± 0.034	0.837LysPhe: 0.837 ± 0.027	1.749LysGly: 1.749 ± 0.042	0.433LysHis: 0.433 ± 0.02	1.266LysIle: 1.266 ± 0.036	0.865LysLys: 0.865 ± 0.044	2.447LysLeu: 2.447 ± 0.054	0.57LysMet: 0.57 ± 0.023	0.671LysAsn: 0.671 ± 0.026	1.274LysPro: 1.274 ± 0.039	0.927LysGln: 0.927 ± 0.034	1.55LysArg: 1.55 ± 0.04	1.508LysSer: 1.508 ± 0.038	1.32LysThr: 1.32 ± 0.035	2.11LysVal: 2.11 ± 0.049	0.342LysTrp: 0.342 ± 0.017	0.501LysTyr: 0.501 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	13.271LeuAla: 13.271 ± 0.151	1.046LeuCys: 1.046 ± 0.031	6.204LeuAsp: 6.204 ± 0.082	5.815LeuGlu: 5.815 ± 0.082	3.15LeuPhe: 3.15 ± 0.063	9.586LeuGly: 9.586 ± 0.113	1.882LeuHis: 1.882 ± 0.042	4.899LeuIle: 4.899 ± 0.078	2.104LeuLys: 2.104 ± 0.051	11.245LeuLeu: 11.245 ± 0.178	1.933LeuMet: 1.933 ± 0.043	2.626LeuAsn: 2.626 ± 0.051	5.571LeuPro: 5.571 ± 0.067	3.52LeuGln: 3.52 ± 0.062	6.981LeuArg: 6.981 ± 0.105	7.161LeuSer: 7.161 ± 0.1	5.902LeuThr: 5.902 ± 0.09	8.513LeuVal: 8.513 ± 0.109	1.304LeuTrp: 1.304 ± 0.041	1.614LeuTyr: 1.614 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.335MetAla: 2.335 ± 0.048	0.207MetCys: 0.207 ± 0.014	0.962MetAsp: 0.962 ± 0.029	0.888MetGlu: 0.888 ± 0.035	0.678MetPhe: 0.678 ± 0.025	1.542MetGly: 1.542 ± 0.037	0.44MetHis: 0.44 ± 0.023	1.022MetIle: 1.022 ± 0.037	0.597MetLys: 0.597 ± 0.025	2.098MetLeu: 2.098 ± 0.053	0.457MetMet: 0.457 ± 0.024	0.683MetAsn: 0.683 ± 0.026	1.021MetPro: 1.021 ± 0.031	0.722MetGln: 0.722 ± 0.025	1.243MetArg: 1.243 ± 0.033	1.747MetSer: 1.747 ± 0.038	1.595MetThr: 1.595 ± 0.04	1.595MetVal: 1.595 ± 0.047	0.268MetTrp: 0.268 ± 0.016	0.395MetTyr: 0.395 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.549AsnAla: 2.549 ± 0.056	0.252AsnCys: 0.252 ± 0.015	1.244AsnAsp: 1.244 ± 0.037	1.365AsnGlu: 1.365 ± 0.036	0.834AsnPhe: 0.834 ± 0.034	2.18AsnGly: 2.18 ± 0.062	0.525AsnHis: 0.525 ± 0.023	0.996AsnIle: 0.996 ± 0.031	0.644AsnLys: 0.644 ± 0.027	2.977AsnLeu: 2.977 ± 0.076	0.516AsnMet: 0.516 ± 0.023	0.761AsnAsn: 0.761 ± 0.035	2.07AsnPro: 2.07 ± 0.048	0.994AsnGln: 0.994 ± 0.037	1.583AsnArg: 1.583 ± 0.043	1.874AsnSer: 1.874 ± 0.047	1.444AsnThr: 1.444 ± 0.047	1.598AsnVal: 1.598 ± 0.04	0.457AsnTrp: 0.457 ± 0.021	0.567AsnTyr: 0.567 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.45ProAla: 6.45 ± 0.108	0.346ProCys: 0.346 ± 0.021	3.508ProAsp: 3.508 ± 0.055	4.523ProGlu: 4.523 ± 0.079	1.669ProPhe: 1.669 ± 0.039	4.681ProGly: 4.681 ± 0.075	0.913ProHis: 0.913 ± 0.033	2.411ProIle: 2.411 ± 0.048	1.486ProLys: 1.486 ± 0.043	4.806ProLeu: 4.806 ± 0.069	1.082ProMet: 1.082 ± 0.032	1.503ProAsn: 1.503 ± 0.039	2.882ProPro: 2.882 ± 0.068	1.859ProGln: 1.859 ± 0.04	2.601ProArg: 2.601 ± 0.049	3.875ProSer: 3.875 ± 0.067	3.757ProThr: 3.757 ± 0.077	4.359ProVal: 4.359 ± 0.072	0.8ProTrp: 0.8 ± 0.026	1.012ProTyr: 1.012 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.466GlnAla: 4.466 ± 0.072	0.256GlnCys: 0.256 ± 0.016	1.735GlnAsp: 1.735 ± 0.043	1.879GlnGlu: 1.879 ± 0.044	1.248GlnPhe: 1.248 ± 0.034	2.6GlnGly: 2.6 ± 0.054	0.7GlnHis: 0.7 ± 0.023	2.182GlnIle: 2.182 ± 0.049	0.813GlnLys: 0.813 ± 0.029	4.187GlnLeu: 4.187 ± 0.069	0.838GlnMet: 0.838 ± 0.033	0.826GlnAsn: 0.826 ± 0.033	2.041GlnPro: 2.041 ± 0.051	1.81GlnGln: 1.81 ± 0.043	2.744GlnArg: 2.744 ± 0.053	2.25GlnSer: 2.25 ± 0.047	1.883GlnThr: 1.883 ± 0.046	3.536GlnVal: 3.536 ± 0.06	0.498GlnTrp: 0.498 ± 0.025	0.668GlnTyr: 0.668 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.073ArgAla: 6.073 ± 0.075	0.567ArgCys: 0.567 ± 0.025	3.162ArgAsp: 3.162 ± 0.064	3.262ArgGlu: 3.262 ± 0.059	2.494ArgPhe: 2.494 ± 0.05	3.99ArgGly: 3.99 ± 0.054	1.284ArgHis: 1.284 ± 0.04	3.202ArgIle: 3.202 ± 0.054	1.689ArgLys: 1.689 ± 0.04	6.441ArgLeu: 6.441 ± 0.098	1.517ArgMet: 1.517 ± 0.041	1.67ArgAsn: 1.67 ± 0.038	3.223ArgPro: 3.223 ± 0.063	2.149ArgGln: 2.149 ± 0.05	4.65ArgArg: 4.65 ± 0.083	4.924ArgSer: 4.924 ± 0.08	3.576ArgThr: 3.576 ± 0.057	5.213ArgVal: 5.213 ± 0.08	1.111ArgTrp: 1.111 ± 0.034	1.474ArgTyr: 1.474 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	8.425SerAla: 8.425 ± 0.109	0.559SerCys: 0.559 ± 0.025	3.83SerAsp: 3.83 ± 0.066	4.32SerGlu: 4.32 ± 0.074	2.305SerPhe: 2.305 ± 0.046	6.768SerGly: 6.768 ± 0.107	1.074SerHis: 1.074 ± 0.033	2.853SerIle: 2.853 ± 0.056	1.628SerLys: 1.628 ± 0.044	6.391SerLeu: 6.391 ± 0.078	1.599SerMet: 1.599 ± 0.043	1.747SerAsn: 1.747 ± 0.044	3.699SerPro: 3.699 ± 0.063	2.371SerGln: 2.371 ± 0.054	3.661SerArg: 3.661 ± 0.062	5.5SerSer: 5.5 ± 0.111	4.486SerThr: 4.486 ± 0.099	5.415SerVal: 5.415 ± 0.079	1.08SerTrp: 1.08 ± 0.034	1.385SerTyr: 1.385 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	7.255ThrAla: 7.255 ± 0.117	0.493ThrCys: 0.493 ± 0.024	3.398ThrAsp: 3.398 ± 0.072	3.702ThrGlu: 3.702 ± 0.069	1.725ThrPhe: 1.725 ± 0.041	5.503ThrGly: 5.503 ± 0.092	1.097ThrHis: 1.097 ± 0.032	2.405ThrIle: 2.405 ± 0.06	1.43ThrLys: 1.43 ± 0.038	5.497ThrLeu: 5.497 ± 0.071	1.124ThrMet: 1.124 ± 0.029	1.564ThrAsn: 1.564 ± 0.041	3.643ThrPro: 3.643 ± 0.067	2.133ThrGln: 2.133 ± 0.043	3.171ThrArg: 3.171 ± 0.06	3.799ThrSer: 3.799 ± 0.072	4.226ThrThr: 4.226 ± 0.13	5.025ThrVal: 5.025 ± 0.096	0.86ThrTrp: 0.86 ± 0.03	1.275ThrTyr: 1.275 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	10.103ValAla: 10.103 ± 0.102	0.769ValCys: 0.769 ± 0.029	4.959ValAsp: 4.959 ± 0.069	4.984ValGlu: 4.984 ± 0.091	2.624ValPhe: 2.624 ± 0.058	6.607ValGly: 6.607 ± 0.089	1.525ValHis: 1.525 ± 0.042	4.231ValIle: 4.231 ± 0.068	1.583ValLys: 1.583 ± 0.04	8.885ValLeu: 8.885 ± 0.119	1.567ValMet: 1.567 ± 0.036	2.168ValAsn: 2.168 ± 0.052	4.289ValPro: 4.289 ± 0.076	2.645ValGln: 2.645 ± 0.053	4.77ValArg: 4.77 ± 0.062	5.115ValSer: 5.115 ± 0.076	5.201ValThr: 5.201 ± 0.086	7.734ValVal: 7.734 ± 0.148	1.026ValTrp: 1.026 ± 0.03	1.269ValTyr: 1.269 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.5TrpAla: 1.5 ± 0.04	0.2TrpCys: 0.2 ± 0.013	0.822TrpAsp: 0.822 ± 0.031	0.701TrpGlu: 0.701 ± 0.027	0.627TrpPhe: 0.627 ± 0.023	1.077TrpGly: 1.077 ± 0.033	0.299TrpHis: 0.299 ± 0.017	0.74TrpIle: 0.74 ± 0.026	0.407TrpLys: 0.407 ± 0.021	1.555TrpLeu: 1.555 ± 0.042	0.414TrpMet: 0.414 ± 0.025	0.47TrpAsn: 0.47 ± 0.024	0.746TrpPro: 0.746 ± 0.025	0.578TrpGln: 0.578 ± 0.022	0.951TrpArg: 0.951 ± 0.034	1.078TrpSer: 1.078 ± 0.034	0.857TrpThr: 0.857 ± 0.031	1.177TrpVal: 1.177 ± 0.036	0.32TrpTrp: 0.32 ± 0.019	0.356TrpTyr: 0.356 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.892TyrAla: 1.892 ± 0.049	0.219TyrCys: 0.219 ± 0.014	1.048TyrAsp: 1.048 ± 0.036	1.288TyrGlu: 1.288 ± 0.043	0.695TyrPhe: 0.695 ± 0.029	1.717TyrGly: 1.717 ± 0.041	0.394TyrHis: 0.394 ± 0.024	0.659TyrIle: 0.659 ± 0.024	0.444TyrLys: 0.444 ± 0.024	2.222TyrLeu: 2.222 ± 0.045	0.325TyrMet: 0.325 ± 0.018	0.511TyrAsn: 0.511 ± 0.028	1.035TyrPro: 1.035 ± 0.038	0.779TyrGln: 0.779 ± 0.029	1.437TyrArg: 1.437 ± 0.037	1.351TyrSer: 1.351 ± 0.034	0.995TyrThr: 0.995 ± 0.034	1.372TyrVal: 1.372 ± 0.037	0.333TyrTrp: 0.333 ± 0.019	0.407TyrTyr: 0.407 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2975 proteins (1027166 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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