Rhizobium sp. AAP43

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0J4Y9|A0A0N0J4Y9_9RHIZ Ureidoacrylate amidohydrolase RutB OS=Rhizobium sp. AAP43 OX=1523420 GN=rutB PE=3 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84LYY5 pKa = 10.24IEE7 pKa = 4.49SKK9 pKa = 10.4AVQQMTVWDD18 pKa = 4.01HH19 pKa = 6.55LYY21 pKa = 10.11IVYY24 pKa = 10.38EE25 pKa = 4.23DD26 pKa = 4.7DD27 pKa = 3.83FGQEE31 pKa = 3.95FVLRR35 pKa = 11.84AGPTSDD41 pKa = 5.65SPLDD45 pKa = 3.42WGSMEE50 pKa = 4.24VEE52 pKa = 4.09IGVPMALSEE61 pKa = 4.13DD62 pKa = 3.38ARR64 pKa = 11.84PIEE67 pKa = 4.84DD68 pKa = 3.62RR69 pKa = 11.84ALHH72 pKa = 6.29GNALLDD78 pKa = 3.79LAGRR82 pKa = 11.84DD83 pKa = 3.48ATAVFQTMMHH93 pKa = 6.38HH94 pKa = 7.35AGNIAQSLLPYY105 pKa = 10.28SAATQNSNSFVASLLHH121 pKa = 6.19VVGIDD126 pKa = 3.64VANVAPDD133 pKa = 3.23TGGIDD138 pKa = 3.31WTPASGNILDD148 pKa = 4.41SIEE151 pKa = 4.19FNVVGGYY158 pKa = 10.8DD159 pKa = 3.45DD160 pKa = 6.22FEE162 pKa = 4.83DD163 pKa = 4.22HH164 pKa = 7.53GDD166 pKa = 3.51ILRR169 pKa = 11.84GGALNDD175 pKa = 4.79AISGRR180 pKa = 11.84AGIDD184 pKa = 3.29QIDD187 pKa = 4.2GGDD190 pKa = 3.94GDD192 pKa = 4.2DD193 pKa = 3.61TLYY196 pKa = 10.87SGSVEE201 pKa = 4.45TPDD204 pKa = 4.63SDD206 pKa = 4.92ADD208 pKa = 3.85YY209 pKa = 9.0LTGGGGVDD217 pKa = 3.32NFYY220 pKa = 11.25VGNSDD225 pKa = 3.84YY226 pKa = 10.22EE227 pKa = 4.45TGLFRR232 pKa = 11.84YY233 pKa = 9.79DD234 pKa = 2.85VDD236 pKa = 3.76SGVMYY241 pKa = 10.46FNSEE245 pKa = 3.51SRR247 pKa = 11.84GRR249 pKa = 11.84YY250 pKa = 9.0DD251 pKa = 3.49VIVDD255 pKa = 4.38FEE257 pKa = 4.59SSDD260 pKa = 4.03AIHH263 pKa = 6.48VSGFGVWDD271 pKa = 3.49YY272 pKa = 11.76TEE274 pKa = 4.87FSSITIDD281 pKa = 3.47DD282 pKa = 3.63AGYY285 pKa = 10.62AIDD288 pKa = 4.78GKK290 pKa = 10.36DD291 pKa = 3.17VYY293 pKa = 10.52VANGSGLEE301 pKa = 4.03LFAVRR306 pKa = 11.84DD307 pKa = 3.71SYY309 pKa = 11.93FDD311 pKa = 3.53VNLEE315 pKa = 3.73RR316 pKa = 11.84DD317 pKa = 3.21IDD319 pKa = 4.56VMVFWYY325 pKa = 10.26QISSRR330 pKa = 11.84MSGMDD335 pKa = 3.72DD336 pKa = 3.33VLIDD340 pKa = 3.59VNIPLFAIDD349 pKa = 4.98YY350 pKa = 7.6GSPWFQQSSSSSFSTADD367 pKa = 3.03ASGSSDD373 pKa = 3.5SIVFQPEE380 pKa = 4.05LVAEE384 pKa = 4.58APSNGYY390 pKa = 9.08DD391 pKa = 3.1QSDD394 pKa = 3.54FSEE397 pKa = 4.75LDD399 pKa = 3.31GLSEE403 pKa = 4.56FGVSHH408 pKa = 6.23ITDD411 pKa = 3.94SIYY414 pKa = 10.92FEE416 pKa = 5.77DD417 pKa = 4.79GFSGIQVHH425 pKa = 7.36DD426 pKa = 4.19GLEE429 pKa = 4.32STHH432 pKa = 5.97QRR434 pKa = 11.84LDD436 pKa = 3.09VDD438 pKa = 3.81EE439 pKa = 4.96FRR441 pKa = 11.84LVAA444 pKa = 4.74

Molecular weight: 48.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0JB90|A0A0N0JB90_9RHIZ Calcium-binding protein OS=Rhizobium sp. AAP43 OX=1523420 GN=IP76_00665 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.84LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.43GGRR29 pKa = 11.84QVLNARR35 pKa = 11.84RR36 pKa = 11.84GRR38 pKa = 11.84GRR40 pKa = 11.84AKK42 pKa = 10.69LSAA45 pKa = 4.0

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4372

0

4372

1394556

41

2949

319.0

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.882 ± 0.048

0.787 ± 0.013

5.835 ± 0.032

5.86 ± 0.034

3.885 ± 0.022

8.34 ± 0.036

2.003 ± 0.018

5.635 ± 0.03

3.578 ± 0.029

10.116 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.019

2.734 ± 0.024

4.814 ± 0.031

3.173 ± 0.023

6.575 ± 0.044

5.793 ± 0.031

5.482 ± 0.031

7.365 ± 0.03

1.243 ± 0.015

2.269 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski