Amino acid dipepetide frequency for Rhizobium sp. AAP43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.407AlaAla: 15.407 ± 0.152
0.936AlaCys: 0.936 ± 0.028
6.926AlaAsp: 6.926 ± 0.087
7.751AlaGlu: 7.751 ± 0.089
4.536AlaPhe: 4.536 ± 0.062
10.009AlaGly: 10.009 ± 0.092
2.102AlaHis: 2.102 ± 0.046
6.747AlaIle: 6.747 ± 0.071
4.258AlaLys: 4.258 ± 0.067
12.741AlaLeu: 12.741 ± 0.139
3.57AlaMet: 3.57 ± 0.053
2.906AlaAsn: 2.906 ± 0.058
4.739AlaPro: 4.739 ± 0.068
3.502AlaGln: 3.502 ± 0.054
7.635AlaArg: 7.635 ± 0.086
6.689AlaSer: 6.689 ± 0.088
5.934AlaThr: 5.934 ± 0.065
8.495AlaVal: 8.495 ± 0.081
1.323AlaTrp: 1.323 ± 0.031
2.611AlaTyr: 2.611 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.83CysAla: 0.83 ± 0.026
0.105CysCys: 0.105 ± 0.008
0.473CysAsp: 0.473 ± 0.02
0.454CysGlu: 0.454 ± 0.019
0.304CysPhe: 0.304 ± 0.017
0.888CysGly: 0.888 ± 0.024
0.234CysHis: 0.234 ± 0.012
0.364CysIle: 0.364 ± 0.014
0.186CysLys: 0.186 ± 0.012
0.787CysLeu: 0.787 ± 0.025
0.148CysMet: 0.148 ± 0.011
0.209CysAsn: 0.209 ± 0.013
0.426CysPro: 0.426 ± 0.022
0.242CysGln: 0.242 ± 0.014
0.574CysArg: 0.574 ± 0.024
0.427CysSer: 0.427 ± 0.017
0.373CysThr: 0.373 ± 0.019
0.546CysVal: 0.546 ± 0.02
0.107CysTrp: 0.107 ± 0.009
0.195CysTyr: 0.195 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.561AspAla: 6.561 ± 0.079
0.532AspCys: 0.532 ± 0.02
3.124AspAsp: 3.124 ± 0.076
3.572AspGlu: 3.572 ± 0.055
2.459AspPhe: 2.459 ± 0.043
5.294AspGly: 5.294 ± 0.099
1.303AspHis: 1.303 ± 0.036
3.43AspIle: 3.43 ± 0.053
1.915AspLys: 1.915 ± 0.04
6.153AspLeu: 6.153 ± 0.074
1.541AspMet: 1.541 ± 0.037
1.421AspAsn: 1.421 ± 0.037
3.309AspPro: 3.309 ± 0.049
1.95AspGln: 1.95 ± 0.044
4.273AspArg: 4.273 ± 0.063
2.143AspSer: 2.143 ± 0.04
2.684AspThr: 2.684 ± 0.054
4.171AspVal: 4.171 ± 0.062
0.929AspTrp: 0.929 ± 0.026
1.586AspTyr: 1.586 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
7.795GluAla: 7.795 ± 0.088
0.354GluCys: 0.354 ± 0.02
3.076GluAsp: 3.076 ± 0.052
3.482GluGlu: 3.482 ± 0.056
1.849GluPhe: 1.849 ± 0.039
4.477GluGly: 4.477 ± 0.068
1.182GluHis: 1.182 ± 0.029
3.975GluIle: 3.975 ± 0.053
2.616GluLys: 2.616 ± 0.05
5.274GluLeu: 5.274 ± 0.067
1.602GluMet: 1.602 ± 0.031
1.824GluAsn: 1.824 ± 0.033
2.637GluPro: 2.637 ± 0.055
2.201GluGln: 2.201 ± 0.043
4.652GluArg: 4.652 ± 0.064
2.303GluSer: 2.303 ± 0.044
3.869GluThr: 3.869 ± 0.054
3.857GluVal: 3.857 ± 0.057
0.632GluTrp: 0.632 ± 0.019
0.94GluTyr: 0.94 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.593PheAla: 4.593 ± 0.061
0.39PheCys: 0.39 ± 0.017
2.84PheAsp: 2.84 ± 0.044
2.289PheGlu: 2.289 ± 0.039
1.564PhePhe: 1.564 ± 0.042
3.742PheGly: 3.742 ± 0.056
0.769PheHis: 0.769 ± 0.021
1.953PheIle: 1.953 ± 0.041
1.16PheLys: 1.16 ± 0.026
3.617PheLeu: 3.617 ± 0.064
0.871PheMet: 0.871 ± 0.026
1.147PheAsn: 1.147 ± 0.028
1.53PhePro: 1.53 ± 0.036
1.13PheGln: 1.13 ± 0.028
2.251PheArg: 2.251 ± 0.043
2.529PheSer: 2.529 ± 0.043
2.014PheThr: 2.014 ± 0.048
2.94PheVal: 2.94 ± 0.053
0.561PheTrp: 0.561 ± 0.024
0.962PheTyr: 0.962 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.424GlyAla: 8.424 ± 0.087
0.76GlyCys: 0.76 ± 0.026
4.368GlyAsp: 4.368 ± 0.077
4.823GlyGlu: 4.823 ± 0.069
3.785GlyPhe: 3.785 ± 0.053
6.831GlyGly: 6.831 ± 0.096
1.892GlyHis: 1.892 ± 0.039
4.849GlyIle: 4.849 ± 0.068
3.583GlyLys: 3.583 ± 0.054
8.938GlyLeu: 8.938 ± 0.093
2.389GlyMet: 2.389 ± 0.04
2.374GlyAsn: 2.374 ± 0.052
3.124GlyPro: 3.124 ± 0.052
2.931GlyGln: 2.931 ± 0.049
5.609GlyArg: 5.609 ± 0.069
4.82GlySer: 4.82 ± 0.079
4.517GlyThr: 4.517 ± 0.063
5.788GlyVal: 5.788 ± 0.08
1.213GlyTrp: 1.213 ± 0.032
2.382GlyTyr: 2.382 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.242HisAla: 2.242 ± 0.044
0.227HisCys: 0.227 ± 0.012
1.244HisAsp: 1.244 ± 0.035
1.062HisGlu: 1.062 ± 0.028
0.894HisPhe: 0.894 ± 0.027
1.862HisGly: 1.862 ± 0.036
0.591HisHis: 0.591 ± 0.025
1.0HisIle: 1.0 ± 0.027
0.521HisLys: 0.521 ± 0.019
2.112HisLeu: 2.112 ± 0.042
0.544HisMet: 0.544 ± 0.02
0.452HisAsn: 0.452 ± 0.019
1.231HisPro: 1.231 ± 0.032
0.635HisGln: 0.635 ± 0.019
1.332HisArg: 1.332 ± 0.033
0.944HisSer: 0.944 ± 0.023
0.783HisThr: 0.783 ± 0.023
1.519HisVal: 1.519 ± 0.036
0.29HisTrp: 0.29 ± 0.017
0.546HisTyr: 0.546 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.725IleAla: 7.725 ± 0.08
0.559IleCys: 0.559 ± 0.017
3.982IleAsp: 3.982 ± 0.06
3.848IleGlu: 3.848 ± 0.054
1.953IlePhe: 1.953 ± 0.051
5.45IleGly: 5.45 ± 0.069
0.957IleHis: 0.957 ± 0.029
2.566IleIle: 2.566 ± 0.049
1.668IleLys: 1.668 ± 0.038
5.099IleLeu: 5.099 ± 0.074
1.149IleMet: 1.149 ± 0.033
1.596IleAsn: 1.596 ± 0.036
2.383IlePro: 2.383 ± 0.048
1.287IleGln: 1.287 ± 0.028
3.588IleArg: 3.588 ± 0.048
3.333IleSer: 3.333 ± 0.056
2.956IleThr: 2.956 ± 0.05
4.328IleVal: 4.328 ± 0.064
0.659IleTrp: 0.659 ± 0.023
1.263IleTyr: 1.263 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.803LysAla: 4.803 ± 0.065
0.168LysCys: 0.168 ± 0.011
1.973LysAsp: 1.973 ± 0.04
1.717LysGlu: 1.717 ± 0.039
0.935LysPhe: 0.935 ± 0.029
2.901LysGly: 2.901 ± 0.045
0.618LysHis: 0.618 ± 0.021
2.044LysIle: 2.044 ± 0.043
1.433LysLys: 1.433 ± 0.044
3.597LysLeu: 3.597 ± 0.052
0.888LysMet: 0.888 ± 0.027
0.984LysAsn: 0.984 ± 0.029
2.143LysPro: 2.143 ± 0.045
1.038LysGln: 1.038 ± 0.03
2.376LysArg: 2.376 ± 0.042
2.115LysSer: 2.115 ± 0.045
2.341LysThr: 2.341 ± 0.042
2.699LysVal: 2.699 ± 0.05
0.369LysTrp: 0.369 ± 0.017
0.632LysTyr: 0.632 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
12.735LeuAla: 12.735 ± 0.116
0.828LeuCys: 0.828 ± 0.026
6.188LeuAsp: 6.188 ± 0.076
5.392LeuGlu: 5.392 ± 0.065
3.741LeuPhe: 3.741 ± 0.07
7.977LeuGly: 7.977 ± 0.087
1.714LeuHis: 1.714 ± 0.041
5.387LeuIle: 5.387 ± 0.08
4.12LeuLys: 4.12 ± 0.052
9.387LeuLeu: 9.387 ± 0.127
2.52LeuMet: 2.52 ± 0.045
2.676LeuAsn: 2.676 ± 0.042
5.4LeuPro: 5.4 ± 0.063
2.84LeuGln: 2.84 ± 0.045
6.087LeuArg: 6.087 ± 0.073
7.351LeuSer: 7.351 ± 0.084
5.905LeuThr: 5.905 ± 0.086
7.653LeuVal: 7.653 ± 0.078
1.126LeuTrp: 1.126 ± 0.032
2.137LeuTyr: 2.137 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.349MetAla: 3.349 ± 0.048
0.141MetCys: 0.141 ± 0.01
1.241MetAsp: 1.241 ± 0.03
1.258MetGlu: 1.258 ± 0.031
0.726MetPhe: 0.726 ± 0.025
1.773MetGly: 1.773 ± 0.042
0.447MetHis: 0.447 ± 0.018
1.611MetIle: 1.611 ± 0.039
1.111MetLys: 1.111 ± 0.034
2.603MetLeu: 2.603 ± 0.047
0.749MetMet: 0.749 ± 0.025
0.836MetAsn: 0.836 ± 0.024
1.537MetPro: 1.537 ± 0.03
0.93MetGln: 0.93 ± 0.024
1.831MetArg: 1.831 ± 0.037
1.76MetSer: 1.76 ± 0.034
2.036MetThr: 2.036 ± 0.038
1.891MetVal: 1.891 ± 0.035
0.209MetTrp: 0.209 ± 0.012
0.272MetTyr: 0.272 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.253AsnAla: 3.253 ± 0.056
0.21AsnCys: 0.21 ± 0.013
1.534AsnAsp: 1.534 ± 0.037
1.358AsnGlu: 1.358 ± 0.029
1.041AsnPhe: 1.041 ± 0.028
2.548AsnGly: 2.548 ± 0.058
0.572AsnHis: 0.572 ± 0.021
1.427AsnIle: 1.427 ± 0.029
0.789AsnLys: 0.789 ± 0.025
2.649AsnLeu: 2.649 ± 0.046
0.703AsnMet: 0.703 ± 0.023
0.749AsnAsn: 0.749 ± 0.028
1.864AsnPro: 1.864 ± 0.042
0.85AsnGln: 0.85 ± 0.023
1.95AsnArg: 1.95 ± 0.033
1.406AsnSer: 1.406 ± 0.034
1.376AsnThr: 1.376 ± 0.033
1.908AsnVal: 1.908 ± 0.039
0.47AsnTrp: 0.47 ± 0.02
0.683AsnTyr: 0.683 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
5.524ProAla: 5.524 ± 0.081
0.272ProCys: 0.272 ± 0.016
3.4ProAsp: 3.4 ± 0.047
3.538ProGlu: 3.538 ± 0.058
1.931ProPhe: 1.931 ± 0.04
4.007ProGly: 4.007 ± 0.06
1.01ProHis: 1.01 ± 0.023
2.386ProIle: 2.386 ± 0.046
1.724ProLys: 1.724 ± 0.037
4.671ProLeu: 4.671 ± 0.067
1.184ProMet: 1.184 ± 0.027
1.252ProAsn: 1.252 ± 0.032
2.064ProPro: 2.064 ± 0.051
1.648ProGln: 1.648 ± 0.034
2.455ProArg: 2.455 ± 0.045
2.76ProSer: 2.76 ± 0.05
2.343ProThr: 2.343 ± 0.041
4.236ProVal: 4.236 ± 0.06
0.572ProTrp: 0.572 ± 0.023
1.166ProTyr: 1.166 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.914GlnAla: 3.914 ± 0.057
0.174GlnCys: 0.174 ± 0.011
1.487GlnAsp: 1.487 ± 0.035
1.608GlnGlu: 1.608 ± 0.04
1.104GlnPhe: 1.104 ± 0.031
2.27GlnGly: 2.27 ± 0.044
0.625GlnHis: 0.625 ± 0.022
2.08GlnIle: 2.08 ± 0.071
1.21GlnLys: 1.21 ± 0.03
2.959GlnLeu: 2.959 ± 0.044
0.97GlnMet: 0.97 ± 0.027
0.904GlnAsn: 0.904 ± 0.027
1.718GlnPro: 1.718 ± 0.039
1.291GlnGln: 1.291 ± 0.036
2.218GlnArg: 2.218 ± 0.04
2.048GlnSer: 2.048 ± 0.042
1.927GlnThr: 1.927 ± 0.034
2.288GlnVal: 2.288 ± 0.05
0.363GlnTrp: 0.363 ± 0.015
0.571GlnTyr: 0.571 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
6.735ArgAla: 6.735 ± 0.076
0.422ArgCys: 0.422 ± 0.017
3.877ArgAsp: 3.877 ± 0.056
3.932ArgGlu: 3.932 ± 0.065
2.936ArgPhe: 2.936 ± 0.047
4.262ArgGly: 4.262 ± 0.051
1.659ArgHis: 1.659 ± 0.035
4.053ArgIle: 4.053 ± 0.05
2.338ArgLys: 2.338 ± 0.041
7.501ArgLeu: 7.501 ± 0.087
1.874ArgMet: 1.874 ± 0.033
1.92ArgAsn: 1.92 ± 0.035
3.118ArgPro: 3.118 ± 0.051
2.663ArgGln: 2.663 ± 0.042
4.922ArgArg: 4.922 ± 0.069
3.78ArgSer: 3.78 ± 0.061
3.042ArgThr: 3.042 ± 0.042
4.135ArgVal: 4.135 ± 0.06
0.842ArgTrp: 0.842 ± 0.028
1.736ArgTyr: 1.736 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.555SerAla: 6.555 ± 0.079
0.411SerCys: 0.411 ± 0.017
3.23SerAsp: 3.23 ± 0.057
3.088SerGlu: 3.088 ± 0.051
2.502SerPhe: 2.502 ± 0.042
5.765SerGly: 5.765 ± 0.082
1.193SerHis: 1.193 ± 0.032
3.185SerIle: 3.185 ± 0.051
1.778SerLys: 1.778 ± 0.037
6.108SerLeu: 6.108 ± 0.077
1.497SerMet: 1.497 ± 0.033
1.531SerAsn: 1.531 ± 0.032
2.665SerPro: 2.665 ± 0.049
1.793SerGln: 1.793 ± 0.039
3.722SerArg: 3.722 ± 0.06
3.372SerSer: 3.372 ± 0.062
3.039SerThr: 3.039 ± 0.054
4.432SerVal: 4.432 ± 0.057
0.736SerTrp: 0.736 ± 0.021
1.326SerTyr: 1.326 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
6.297ThrAla: 6.297 ± 0.075
0.414ThrCys: 0.414 ± 0.023
3.091ThrAsp: 3.091 ± 0.052
2.886ThrGlu: 2.886 ± 0.046
2.164ThrPhe: 2.164 ± 0.044
5.13ThrGly: 5.13 ± 0.064
1.011ThrHis: 1.011 ± 0.027
3.214ThrIle: 3.214 ± 0.051
1.608ThrLys: 1.608 ± 0.036
5.904ThrLeu: 5.904 ± 0.082
1.302ThrMet: 1.302 ± 0.026
1.451ThrAsn: 1.451 ± 0.033
3.017ThrPro: 3.017 ± 0.05
1.474ThrGln: 1.474 ± 0.041
3.197ThrArg: 3.197 ± 0.042
3.116ThrSer: 3.116 ± 0.057
3.042ThrThr: 3.042 ± 0.068
4.561ThrVal: 4.561 ± 0.064
0.65ThrTrp: 0.65 ± 0.024
1.29ThrTyr: 1.29 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
8.631ValAla: 8.631 ± 0.093
0.599ValCys: 0.599 ± 0.021
4.257ValAsp: 4.257 ± 0.058
4.74ValGlu: 4.74 ± 0.058
2.903ValPhe: 2.903 ± 0.052
5.359ValGly: 5.359 ± 0.074
1.347ValHis: 1.347 ± 0.034
4.452ValIle: 4.452 ± 0.057
2.544ValLys: 2.544 ± 0.052
7.176ValLeu: 7.176 ± 0.07
1.971ValMet: 1.971 ± 0.038
2.063ValAsn: 2.063 ± 0.039
3.438ValPro: 3.438 ± 0.047
1.984ValGln: 1.984 ± 0.041
4.373ValArg: 4.373 ± 0.058
4.913ValSer: 4.913 ± 0.061
4.764ValThr: 4.764 ± 0.063
5.793ValVal: 5.793 ± 0.08
0.833ValTrp: 0.833 ± 0.027
1.514ValTyr: 1.514 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.059TrpAla: 1.059 ± 0.032
0.133TrpCys: 0.133 ± 0.009
0.6TrpAsp: 0.6 ± 0.021
0.539TrpGlu: 0.539 ± 0.019
0.54TrpPhe: 0.54 ± 0.02
0.774TrpGly: 0.774 ± 0.025
0.323TrpHis: 0.323 ± 0.016
0.683TrpIle: 0.683 ± 0.02
0.502TrpLys: 0.502 ± 0.019
1.524TrpLeu: 1.524 ± 0.039
0.359TrpMet: 0.359 ± 0.016
0.451TrpAsn: 0.451 ± 0.02
0.647TrpPro: 0.647 ± 0.023
0.567TrpGln: 0.567 ± 0.018
0.947TrpArg: 0.947 ± 0.026
0.822TrpSer: 0.822 ± 0.027
0.736TrpThr: 0.736 ± 0.024
0.724TrpVal: 0.724 ± 0.023
0.207TrpTrp: 0.207 ± 0.011
0.29TrpTyr: 0.29 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.388TyrAla: 2.388 ± 0.041
0.236TyrCys: 0.236 ± 0.011
1.44TyrAsp: 1.44 ± 0.038
1.262TyrGlu: 1.262 ± 0.034
0.984TyrPhe: 0.984 ± 0.027
2.08TyrGly: 2.08 ± 0.039
0.48TyrHis: 0.48 ± 0.02
0.942TyrIle: 0.942 ± 0.029
0.711TyrLys: 0.711 ± 0.026
2.365TyrLeu: 2.365 ± 0.045
0.478TyrMet: 0.478 ± 0.018
0.594TyrAsn: 0.594 ± 0.021
1.153TyrPro: 1.153 ± 0.029
0.769TyrGln: 0.769 ± 0.024
1.755TyrArg: 1.755 ± 0.043
1.296TyrSer: 1.296 ± 0.036
1.142TyrThr: 1.142 ± 0.031
1.69TyrVal: 1.69 ± 0.039
0.336TyrTrp: 0.336 ± 0.015
0.59TyrTyr: 0.59 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4372 proteins (1394556 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski