Paracoccus homiensis
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9ZXL6|A0A1H9ZXL6_9RHOB Nucleoside-binding protein OS=Paracoccus homiensis OX=364199 GN=SAMN04489858_10230 PE=4 SV=1
MM1 pKa = 7.33 ATLFGNSGNNTIPGTSGADD20 pKa = 3.78 LIYY23 pKa = 10.85 GFGGNDD29 pKa = 3.16 SLGGNGGRR37 pKa = 11.84 DD38 pKa = 4.01 TIWGGSGNDD47 pKa = 3.84 TIRR50 pKa = 11.84 SSGAGVYY57 pKa = 10.51 DD58 pKa = 3.98 GGTGNDD64 pKa = 3.3 YY65 pKa = 11.21 VYY67 pKa = 11.06 AGLGTQEE74 pKa = 4.28 TLRR77 pKa = 11.84 GGAGTDD83 pKa = 3.35 WLNTTHH89 pKa = 7.24 WNGNYY94 pKa = 8.96 TIYY97 pKa = 8.03 MTTGATNHH105 pKa = 6.17 TGEE108 pKa = 5.1 SFTQFEE114 pKa = 4.46 NLVTGNGNDD123 pKa = 3.96 NITGTSGANRR133 pKa = 11.84 IYY135 pKa = 10.37 TYY137 pKa = 10.99 GGNDD141 pKa = 3.13 RR142 pKa = 11.84 VNAGAGNDD150 pKa = 3.95 YY151 pKa = 11.08 VWTGDD156 pKa = 3.66 GNDD159 pKa = 4.49 SIDD162 pKa = 3.6 AGSGRR167 pKa = 11.84 DD168 pKa = 3.33 TVYY171 pKa = 11.04 GGNGNDD177 pKa = 4.23 TIRR180 pKa = 11.84 SSGSGIFDD188 pKa = 3.41 GQGGNDD194 pKa = 3.86 YY195 pKa = 10.43 IYY197 pKa = 11.02 AGLGTQEE204 pKa = 4.28 TLRR207 pKa = 11.84 GGAGTDD213 pKa = 3.35 WLNTTHH219 pKa = 7.03 WNSDD223 pKa = 3.17 YY224 pKa = 10.31 TINMVTGATNYY235 pKa = 9.07 TGEE238 pKa = 4.48 SFTQFEE244 pKa = 4.46 NLVTGNGDD252 pKa = 3.6 DD253 pKa = 4.72 NIIGTADD260 pKa = 3.4 GNRR263 pKa = 11.84 IYY265 pKa = 11.06 TNGGNDD271 pKa = 3.5 KK272 pKa = 10.64 VDD274 pKa = 3.46 AGAGNDD280 pKa = 3.98 YY281 pKa = 11.15 VWTANGNDD289 pKa = 4.17 SIDD292 pKa = 3.63 AGSGRR297 pKa = 11.84 DD298 pKa = 3.33 TVYY301 pKa = 11.04 GGNGNDD307 pKa = 4.23 TIRR310 pKa = 11.84 SSGSGIFDD318 pKa = 3.41 GQGDD322 pKa = 4.01 NDD324 pKa = 3.74 YY325 pKa = 11.39 VYY327 pKa = 10.98 AGLGAQEE334 pKa = 4.28 TLRR337 pKa = 11.84 GGAGTDD343 pKa = 3.35 WLNTTHH349 pKa = 7.24 WNGNYY354 pKa = 8.55 TINMTTGATNYY365 pKa = 8.91 TGEE368 pKa = 4.48 SFTQFEE374 pKa = 4.46 NLVTGNGNDD383 pKa = 3.96 NITGTSGANRR393 pKa = 11.84 IYY395 pKa = 10.7 TRR397 pKa = 11.84 GGNDD401 pKa = 2.85 TVAAGAGNDD410 pKa = 3.95 YY411 pKa = 11.18 VSGGAGNDD419 pKa = 3.66 SLDD422 pKa = 4.05 GGSGTNTVYY431 pKa = 10.73 GGSGDD436 pKa = 3.86 DD437 pKa = 4.04 VIRR440 pKa = 11.84 SSGRR444 pKa = 11.84 GVYY447 pKa = 10.13 DD448 pKa = 3.67 GQDD451 pKa = 3.66 GNDD454 pKa = 4.41 LIYY457 pKa = 10.86 AGNGTPEE464 pKa = 4.19 TLRR467 pKa = 11.84 GGSGIDD473 pKa = 3.46 TLNTTSFGGDD483 pKa = 3.48 YY484 pKa = 10.48 NIDD487 pKa = 3.55 MATGTTNFSGEE498 pKa = 4.15 SFTQFEE504 pKa = 4.47 HH505 pKa = 7.03 LIAGAGDD512 pKa = 3.48 DD513 pKa = 3.88 TLAGNSASNRR523 pKa = 11.84 ISGGDD528 pKa = 3.35 GNDD531 pKa = 3.93 RR532 pKa = 11.84 ITGLDD537 pKa = 3.78 GNDD540 pKa = 3.33 TLYY543 pKa = 11.43 GDD545 pKa = 4.3 NGNDD549 pKa = 3.46 YY550 pKa = 11.28 LSGGNGNDD558 pKa = 3.95 LLFGGNGNDD567 pKa = 3.34 TMLGGSGNDD576 pKa = 2.62 RR577 pKa = 11.84 MYY579 pKa = 11.4 GNNGVDD585 pKa = 3.14 RR586 pKa = 11.84 MFGGSGNDD594 pKa = 3.38 YY595 pKa = 10.44 MRR597 pKa = 11.84 GGNDD601 pKa = 3.02 NDD603 pKa = 3.71 RR604 pKa = 11.84 LYY606 pKa = 11.01 GQNGRR611 pKa = 11.84 DD612 pKa = 3.53 NMYY615 pKa = 11.16 GDD617 pKa = 4.62 AGNDD621 pKa = 3.05 TMYY624 pKa = 11.05 GGNQNDD630 pKa = 3.71 RR631 pKa = 11.84 MDD633 pKa = 4.24 GGSGNDD639 pKa = 3.27 TMFGQSGDD647 pKa = 3.56 DD648 pKa = 3.69 RR649 pKa = 11.84 ISGRR653 pKa = 11.84 LGADD657 pKa = 3.28 VMSGGGGEE665 pKa = 4.23 DD666 pKa = 3.1 TFVFYY671 pKa = 10.41 STADD675 pKa = 3.52 SPFGNSANYY684 pKa = 10.28 DD685 pKa = 4.04 RR686 pKa = 11.84 ITDD689 pKa = 3.88 FQGAGINLLSTIEE702 pKa = 4.19 DD703 pKa = 4.31 KK704 pKa = 10.93 IDD706 pKa = 3.98 LSAIDD711 pKa = 4.73 ANTGIAGNQAFTFSGTSNGGAGSIWMQNVGSEE743 pKa = 3.52 TWLRR747 pKa = 11.84 VNTDD751 pKa = 3.06 ADD753 pKa = 3.94 STPEE757 pKa = 3.6 MTIRR761 pKa = 11.84 ISDD764 pKa = 3.85 GADD767 pKa = 3.09 DD768 pKa = 5.98 ANDD771 pKa = 3.13 YY772 pKa = 8.84 WAGDD776 pKa = 4.15 FILL779 pKa = 5.43
Molecular weight: 80.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.676
IPC2_protein 3.643
IPC_protein 3.719
Toseland 3.465
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.528
Grimsley 3.363
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.164
Thurlkill 3.528
EMBOSS 3.706
Sillero 3.834
Patrickios 0.973
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A1H9Y8F2|A0A1H9Y8F2_9RHOB Protein RodZ contains Xre-like HTH and DUF4115 domains OS=Paracoccus homiensis OX=364199 GN=SAMN04489858_10158 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 KK40 pKa = 8.91 SLSAA44 pKa = 3.86
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3828
0
3828
1177105
27
4492
307.5
33.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.61 ± 0.058
0.867 ± 0.013
6.428 ± 0.041
5.237 ± 0.043
3.548 ± 0.029
8.939 ± 0.083
2.069 ± 0.023
5.288 ± 0.03
2.734 ± 0.033
10.078 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.852 ± 0.023
2.466 ± 0.024
5.222 ± 0.03
3.654 ± 0.026
7.184 ± 0.047
5.027 ± 0.034
5.265 ± 0.026
7.0 ± 0.035
1.459 ± 0.019
2.074 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here