Sulfuricaulis limicola
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2756 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4XEQ1|A0A1B4XEQ1_9GAMM Uncharacterized protein OS=Sulfuricaulis limicola OX=1620215 GN=SCL_0963 PE=4 SV=1
MM1 pKa = 7.31 FRR3 pKa = 11.84 TITWFLIVSLFTLNMAWAIDD23 pKa = 3.59 NCAFSDD29 pKa = 4.3 PSDD32 pKa = 3.61 SGAGMTQPFDD42 pKa = 3.84 PAPEE46 pKa = 5.23 DD47 pKa = 3.71 STSTTPACGQWCPGWVSLVTLPVSQVLLPSLLSTFEE83 pKa = 4.27 GGFGADD89 pKa = 3.42 PYY91 pKa = 11.3 VFLPAPPPIHH101 pKa = 6.83 PPIAA105 pKa = 4.75
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 3.681
IPC_protein 3.541
Toseland 3.363
ProMoST 3.795
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.287
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.694
Patrickios 0.172
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.667
Protein with the highest isoelectric point:
>tr|A0A1B4XC22|A0A1B4XC22_9GAMM Dihydrolipoyl dehydrogenase OS=Sulfuricaulis limicola OX=1620215 GN=SCL_0043 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.74 RR14 pKa = 11.84 THH16 pKa = 5.77 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.06 TTGGRR28 pKa = 11.84 RR29 pKa = 11.84 ILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2756
0
2756
850570
40
2923
308.6
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.788 ± 0.052
0.948 ± 0.017
5.191 ± 0.035
5.948 ± 0.05
3.7 ± 0.032
7.996 ± 0.045
2.445 ± 0.023
5.231 ± 0.039
4.167 ± 0.046
10.576 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.515 ± 0.025
3.013 ± 0.031
5.018 ± 0.035
3.549 ± 0.029
7.149 ± 0.044
5.166 ± 0.037
5.231 ± 0.033
7.344 ± 0.039
1.348 ± 0.02
2.675 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here