Cohnella sp. AR92
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5378 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S0YPN6|A0A3S0YPN6_9BACL Cell wall-active antibiotics response protein OS=Cohnella sp. AR92 OX=648716 GN=ELR57_19485 PE=4 SV=1
MM1 pKa = 7.26 SVVVCPWCQSEE12 pKa = 4.4 IPQEE16 pKa = 4.17 EE17 pKa = 4.45 GQEE20 pKa = 4.04 PEE22 pKa = 4.7 KK23 pKa = 10.99 YY24 pKa = 10.52 CPVCEE29 pKa = 4.38 NEE31 pKa = 3.65 LDD33 pKa = 3.74 GYY35 pKa = 8.82 RR36 pKa = 11.84 TLRR39 pKa = 11.84 IGLGDD44 pKa = 4.19 EE45 pKa = 4.89 DD46 pKa = 6.29 DD47 pKa = 4.08 SDD49 pKa = 4.11 EE50 pKa = 5.58 DD51 pKa = 4.96 FEE53 pKa = 6.2 EE54 pKa = 6.46 DD55 pKa = 4.82 DD56 pKa = 5.41 DD57 pKa = 5.94 DD58 pKa = 5.12 SSSYY62 pKa = 11.13 GAILTDD68 pKa = 3.49 EE69 pKa = 4.39 EE70 pKa = 5.35 DD71 pKa = 3.28 ISFEE75 pKa = 4.11 QDD77 pKa = 2.12 EE78 pKa = 4.41 DD79 pKa = 4.07 LQVKK83 pKa = 10.26 NEE85 pKa = 3.82 ALMQYY90 pKa = 10.24 EE91 pKa = 4.21 EE92 pKa = 4.47 TVEE95 pKa = 4.19 KK96 pKa = 10.8 LLDD99 pKa = 3.75 EE100 pKa = 4.37 QEE102 pKa = 4.61 TVPEE106 pKa = 4.47 CPQCRR111 pKa = 11.84 EE112 pKa = 3.9 YY113 pKa = 10.56 MLEE116 pKa = 4.12 AGEE119 pKa = 4.48 TTVPAAGFLARR130 pKa = 11.84 VPEE133 pKa = 4.26 SLGAPILEE141 pKa = 4.85 APYY144 pKa = 10.78 SMKK147 pKa = 10.62 VYY149 pKa = 10.32 VCPSCFQVQHH159 pKa = 6.19 SLSDD163 pKa = 3.19 EE164 pKa = 3.96 HH165 pKa = 6.51 RR166 pKa = 11.84 TILIQKK172 pKa = 9.01 LAANRR177 pKa = 11.84 ARR179 pKa = 4.04
Molecular weight: 20.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.681
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.694
Rodwell 3.694
Grimsley 3.592
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.075
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.973
Patrickios 0.871
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A433RMJ5|A0A433RMJ5_9BACL Zinc ribbon domain-containing protein OS=Cohnella sp. AR92 OX=648716 GN=ELR57_01050 PE=4 SV=1
MM1 pKa = 7.61 GPTFKK6 pKa = 10.87 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.57 KK14 pKa = 8.17 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSSKK25 pKa = 10.58 NGRR28 pKa = 11.84 KK29 pKa = 8.51 VLKK32 pKa = 10.34 ARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.21 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.067
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.676
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.398
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5378
0
5378
1738542
26
2988
323.3
35.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.134 ± 0.04
0.701 ± 0.01
5.194 ± 0.026
6.896 ± 0.038
3.96 ± 0.024
7.821 ± 0.033
1.87 ± 0.016
6.109 ± 0.027
4.957 ± 0.031
10.26 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.57 ± 0.017
3.552 ± 0.026
4.216 ± 0.021
3.418 ± 0.02
5.843 ± 0.042
6.651 ± 0.033
5.176 ± 0.032
6.998 ± 0.03
1.34 ± 0.014
3.332 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here