Macrophomina phaseolina chrysovirus 1
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4KRA2|A0A0M4KRA2_9VIRU p3 OS=Macrophomina phaseolina chrysovirus 1 OX=1708483 PE=4 SV=1
MM1 pKa = 6.96 SQYY4 pKa = 11.12 NRR6 pKa = 11.84 VADD9 pKa = 4.1 YY10 pKa = 11.42 LSGLPDD16 pKa = 3.83 AVNHH20 pKa = 5.48 MDD22 pKa = 3.53 RR23 pKa = 11.84 YY24 pKa = 8.31 VTEE27 pKa = 3.82 FLEE30 pKa = 4.23 RR31 pKa = 11.84 HH32 pKa = 4.48 STSRR36 pKa = 11.84 NVIIDD41 pKa = 3.43 GVEE44 pKa = 3.7 AEE46 pKa = 4.4 KK47 pKa = 10.93 QILSPAEE54 pKa = 3.85 RR55 pKa = 11.84 NVIRR59 pKa = 11.84 ALNCQLKK66 pKa = 8.26 FTKK69 pKa = 10.34 AHH71 pKa = 6.48 FGDD74 pKa = 4.25 GFNLSVRR81 pKa = 11.84 AVARR85 pKa = 11.84 HH86 pKa = 5.07 KK87 pKa = 10.93 NSYY90 pKa = 10.12 KK91 pKa = 10.79 DD92 pKa = 3.46 RR93 pKa = 11.84 TDD95 pKa = 3.24 KK96 pKa = 11.3 LSTIQLSFRR105 pKa = 11.84 GSRR108 pKa = 11.84 EE109 pKa = 3.78 WQLLPSRR116 pKa = 11.84 ATVMYY121 pKa = 8.27 ATAEE125 pKa = 4.17 SSMHH129 pKa = 7.27 LMDD132 pKa = 5.33 DD133 pKa = 3.4 QIKK136 pKa = 9.4 RR137 pKa = 11.84 HH138 pKa = 5.44 ILARR142 pKa = 11.84 CHH144 pKa = 5.88 TMLSSEE150 pKa = 4.27 TSSLPMAAVSEE161 pKa = 4.28 KK162 pKa = 9.86 MQIPMDD168 pKa = 3.85 MRR170 pKa = 11.84 HH171 pKa = 5.88 MFMKK175 pKa = 9.94 MYY177 pKa = 9.98 LYY179 pKa = 11.24 LMDD182 pKa = 3.82 YY183 pKa = 10.76 SLAVTSKK190 pKa = 7.9 STTRR194 pKa = 11.84 EE195 pKa = 3.69 VCDD198 pKa = 4.61 DD199 pKa = 3.95 IEE201 pKa = 4.43 YY202 pKa = 10.44 QGYY205 pKa = 9.25 PNVSQAARR213 pKa = 11.84 ISAMMDD219 pKa = 3.21 RR220 pKa = 11.84 SIVIDD225 pKa = 3.74 TDD227 pKa = 3.16 GMTPEE232 pKa = 3.99 EE233 pKa = 4.74 LGLLVLSSNEE243 pKa = 3.99 YY244 pKa = 9.49 PSVFYY249 pKa = 10.67 GSEE252 pKa = 3.95 NIYY255 pKa = 9.27 TNCSMEE261 pKa = 4.59 KK262 pKa = 10.29 DD263 pKa = 3.75 DD264 pKa = 5.12 MVLVSDD270 pKa = 4.54 GDD272 pKa = 3.91 IVVDD276 pKa = 5.42 KK277 pKa = 10.79 SYY279 pKa = 11.09 LWGSPDD285 pKa = 3.77 RR286 pKa = 11.84 LYY288 pKa = 11.68 NLIWSVATKK297 pKa = 10.11 LDD299 pKa = 3.94 CVASLVQAFRR309 pKa = 11.84 MMRR312 pKa = 11.84 GMPKK316 pKa = 9.08 MASDD320 pKa = 3.21 MFSRR324 pKa = 11.84 VKK326 pKa = 10.44 EE327 pKa = 4.38 SNTILSMVPPSHH339 pKa = 6.7 SLTRR343 pKa = 11.84 ALGEE347 pKa = 3.87 IPIRR351 pKa = 11.84 PSLSRR356 pKa = 11.84 APGWFSTSFSLATDD370 pKa = 4.83 LIYY373 pKa = 10.95 GSMFEE378 pKa = 4.62 AVATNVVEE386 pKa = 4.14 EE387 pKa = 4.46 LGGIGKK393 pKa = 9.51 ALSSATPATSRR404 pKa = 11.84 VFNGLLRR411 pKa = 11.84 DD412 pKa = 3.9 YY413 pKa = 10.67 GLEE416 pKa = 4.15 HH417 pKa = 7.69 ADD419 pKa = 3.23 PTVNVLLQGWNSISGTVIKK438 pKa = 10.09 WGFGKK443 pKa = 9.25 MLKK446 pKa = 10.12 DD447 pKa = 3.6 YY448 pKa = 10.89 CVGLAGEE455 pKa = 4.44 IQNGLDD461 pKa = 2.87 IRR463 pKa = 11.84 MPQIMALIPYY473 pKa = 9.16 CSTPNTVWGYY483 pKa = 9.53 SRR485 pKa = 11.84 GWTGTSDD492 pKa = 4.56 LLEE495 pKa = 4.33 LSKK498 pKa = 10.67 KK499 pKa = 10.03 ARR501 pKa = 11.84 RR502 pKa = 11.84 EE503 pKa = 3.97 QTNKK507 pKa = 9.59 LAAVAWVLGIRR518 pKa = 11.84 EE519 pKa = 3.77 VRR521 pKa = 11.84 PRR523 pKa = 11.84 VFRR526 pKa = 11.84 NTFSTEE532 pKa = 3.73 EE533 pKa = 4.07 LTLSPLEE540 pKa = 4.09 YY541 pKa = 10.13 EE542 pKa = 4.66 LASEE546 pKa = 4.57 ASNGCEE552 pKa = 5.01 LEE554 pKa = 5.63 DD555 pKa = 3.68 VQLWLADD562 pKa = 3.82 SVGGRR567 pKa = 11.84 VDD569 pKa = 3.69 EE570 pKa = 4.37 QEE572 pKa = 4.05 KK573 pKa = 8.13 TASGLTRR580 pKa = 11.84 TEE582 pKa = 3.8 YY583 pKa = 11.05 AGAKK587 pKa = 9.84 CSLIYY592 pKa = 9.43 DD593 pKa = 4.57 TIEE596 pKa = 4.1 KK597 pKa = 9.92 QWVIDD602 pKa = 4.26 DD603 pKa = 4.35 KK604 pKa = 11.65 KK605 pKa = 9.7 PDD607 pKa = 3.46 YY608 pKa = 10.91 RR609 pKa = 11.84 KK610 pKa = 9.04 MVSEE614 pKa = 4.16 SLKK617 pKa = 10.92 GEE619 pKa = 4.03 PKK621 pKa = 10.32 AEE623 pKa = 4.21 KK624 pKa = 10.38 LEE626 pKa = 4.17 EE627 pKa = 4.0 MDD629 pKa = 3.42 GLKK632 pKa = 10.39 PVTFGGIPTPQDD644 pKa = 3.22 PFEE647 pKa = 4.81 HH648 pKa = 6.18 LKK650 pKa = 11.22 SMNKK654 pKa = 8.69 TSLIRR659 pKa = 11.84 PSAGPRR665 pKa = 11.84 HH666 pKa = 5.71 FRR668 pKa = 11.84 MGSDD672 pKa = 2.97 MTPFVPEE679 pKa = 3.76 YY680 pKa = 10.58 VVDD683 pKa = 4.18 GEE685 pKa = 4.55 RR686 pKa = 11.84 EE687 pKa = 4.2 SGLLTRR693 pKa = 11.84 TRR695 pKa = 11.84 LNVKK699 pKa = 9.79 EE700 pKa = 4.01 GDD702 pKa = 3.63 EE703 pKa = 4.17 IEE705 pKa = 4.1 IKK707 pKa = 10.18 EE708 pKa = 4.19 ISVMHH713 pKa = 6.98 DD714 pKa = 3.81 GSCGIHH720 pKa = 6.86 SIVEE724 pKa = 4.09 DD725 pKa = 3.53 LRR727 pKa = 11.84 VRR729 pKa = 11.84 GMLDD733 pKa = 2.76 TSDD736 pKa = 3.39 ARR738 pKa = 11.84 KK739 pKa = 9.62 ASEE742 pKa = 3.93 MFAEE746 pKa = 5.4 GTASKK751 pKa = 10.61 NFHH754 pKa = 6.37 DD755 pKa = 4.28 TEE757 pKa = 4.04 EE758 pKa = 4.08 LAALAEE764 pKa = 4.03 QWGFGLSVINEE775 pKa = 4.32 DD776 pKa = 3.48 GNVYY780 pKa = 10.66 KK781 pKa = 10.87 FGDD784 pKa = 3.76 GKK786 pKa = 10.41 QEE788 pKa = 3.71 HH789 pKa = 7.71 DD790 pKa = 3.14 IVIARR795 pKa = 11.84 RR796 pKa = 11.84 GLSFQPAIIGKK807 pKa = 9.52 GGRR810 pKa = 11.84 KK811 pKa = 9.18 LKK813 pKa = 10.6 SNKK816 pKa = 8.71 IHH818 pKa = 6.35 EE819 pKa = 4.36 QTAPNDD825 pKa = 3.32 EE826 pKa = 5.22 FIAKK830 pKa = 8.58 VKK832 pKa = 9.87 EE833 pKa = 3.68 MRR835 pKa = 11.84 HH836 pKa = 4.73 IFGAVRR842 pKa = 11.84 RR843 pKa = 4.14
Molecular weight: 94.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.654
IPC2_protein 5.614
IPC_protein 5.639
Toseland 5.957
ProMoST 5.944
Dawson 5.83
Bjellqvist 5.868
Wikipedia 5.804
Rodwell 5.804
Grimsley 6.084
Solomon 5.83
Lehninger 5.817
Nozaki 6.071
DTASelect 6.249
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.16
Patrickios 4.317
IPC_peptide 5.842
IPC2_peptide 6.16
IPC2.peptide.svr19 6.143
Protein with the highest isoelectric point:
>tr|A0A0M4LD02|A0A0M4LD02_9VIRU Capsid protein OS=Macrophomina phaseolina chrysovirus 1 OX=1708483 PE=4 SV=2
MM1 pKa = 7.98 DD2 pKa = 4.88 FSNLLKK8 pKa = 10.65 HH9 pKa = 6.28 LAGGQRR15 pKa = 11.84 TEE17 pKa = 3.81 RR18 pKa = 11.84 GKK20 pKa = 11.08 GKK22 pKa = 9.7 RR23 pKa = 11.84 DD24 pKa = 3.17 KK25 pKa = 11.3 NEE27 pKa = 3.88 RR28 pKa = 11.84 NSGRR32 pKa = 11.84 RR33 pKa = 11.84 TDD35 pKa = 3.66 KK36 pKa = 10.85 KK37 pKa = 9.85 MKK39 pKa = 9.31 RR40 pKa = 11.84 TGGMSGKK47 pKa = 9.61 VFQCAKK53 pKa = 10.61 PEE55 pKa = 3.98 MPDD58 pKa = 3.26 CDD60 pKa = 3.25 EE61 pKa = 3.96 WDD63 pKa = 3.68 GNRR66 pKa = 11.84 EE67 pKa = 3.83 NFIGIVVPPGGGKK80 pKa = 8.29 TYY82 pKa = 10.18 MADD85 pKa = 3.0 KK86 pKa = 10.63 FGWLDD91 pKa = 3.7 ADD93 pKa = 4.54 DD94 pKa = 6.07 LIDD97 pKa = 4.48 RR98 pKa = 11.84 LDD100 pKa = 3.8 RR101 pKa = 11.84 EE102 pKa = 4.18 QLRR105 pKa = 11.84 DD106 pKa = 3.46 VVMNNVAAGMSVSEE120 pKa = 5.31 AIRR123 pKa = 11.84 VLDD126 pKa = 3.68 GRR128 pKa = 11.84 RR129 pKa = 11.84 KK130 pKa = 8.19 EE131 pKa = 4.36 TLNLLYY137 pKa = 9.79 PWTTQVVLAHH147 pKa = 6.33 SVQSLEE153 pKa = 4.37 QMGVDD158 pKa = 3.34 CMGRR162 pKa = 11.84 IQLWEE167 pKa = 4.06 PLMEE171 pKa = 5.33 AGLKK175 pKa = 10.07 GRR177 pKa = 11.84 EE178 pKa = 3.77 EE179 pKa = 4.19 HH180 pKa = 6.47 EE181 pKa = 4.23 KK182 pKa = 10.87 VFARR186 pKa = 11.84 MMNKK190 pKa = 9.8 FSEE193 pKa = 4.46 QEE195 pKa = 3.9 KK196 pKa = 9.54 SHH198 pKa = 6.43 VPLFPAHH205 pKa = 6.57 KK206 pKa = 9.33 HH207 pKa = 5.82 RR208 pKa = 11.84 DD209 pKa = 3.7 VICYY213 pKa = 9.29 IMKK216 pKa = 9.68 VCGYY220 pKa = 9.93 CDD222 pKa = 3.2 IPVPVPHH229 pKa = 7.14 LYY231 pKa = 10.35 DD232 pKa = 4.83 GEE234 pKa = 4.5 MIGYY238 pKa = 7.44 TMPEE242 pKa = 3.9 LLASEE247 pKa = 4.09 NLALIIEE254 pKa = 4.49 AEE256 pKa = 4.1 KK257 pKa = 9.83 QARR260 pKa = 11.84 VPRR263 pKa = 11.84 CHH265 pKa = 6.44 VDD267 pKa = 3.2 YY268 pKa = 10.33 MVRR271 pKa = 11.84 EE272 pKa = 4.01 QGMRR276 pKa = 11.84 SYY278 pKa = 9.79 MGYY281 pKa = 10.6 GVTVGDD287 pKa = 3.84 WARR290 pKa = 11.84 ALAKK294 pKa = 10.23 CSHH297 pKa = 6.59 TNASDD302 pKa = 3.44 LKK304 pKa = 10.49 DD305 pKa = 3.39 IEE307 pKa = 4.19 GTIYY311 pKa = 10.6 RR312 pKa = 11.84 GLDD315 pKa = 3.17 LARR318 pKa = 11.84 ASEE321 pKa = 3.95 ITDD324 pKa = 3.26 IAEE327 pKa = 4.44 HH328 pKa = 6.96 EE329 pKa = 4.4 DD330 pKa = 3.35 VQQILKK336 pKa = 8.35 MQEE339 pKa = 3.72 NAEE342 pKa = 4.41 SMFALNLIMHH352 pKa = 7.13 WKK354 pKa = 8.25 MVGIRR359 pKa = 11.84 SPLRR363 pKa = 11.84 SSIFRR368 pKa = 11.84 LYY370 pKa = 10.59 GVHH373 pKa = 4.39 QCRR376 pKa = 11.84 WSRR379 pKa = 11.84 AMGAVRR385 pKa = 11.84 DD386 pKa = 4.18 VVKK389 pKa = 8.39 MTDD392 pKa = 3.1 NLLGHH397 pKa = 7.07 PLDD400 pKa = 3.92 EE401 pKa = 4.78 DD402 pKa = 3.54 EE403 pKa = 5.94 RR404 pKa = 11.84 RR405 pKa = 11.84 TLSEE409 pKa = 3.94 LWWLGRR415 pKa = 11.84 YY416 pKa = 8.4 KK417 pKa = 10.4 RR418 pKa = 11.84 RR419 pKa = 11.84 SFMDD423 pKa = 3.28 TLSQSGRR430 pKa = 11.84 SGYY433 pKa = 10.67 ARR435 pKa = 11.84 RR436 pKa = 11.84 CSPAMLRR443 pKa = 11.84 EE444 pKa = 4.2 AKK446 pKa = 10.26 KK447 pKa = 10.31 SFRR450 pKa = 11.84 MLYY453 pKa = 9.47 TKK455 pKa = 10.72 DD456 pKa = 3.09 EE457 pKa = 4.17 TRR459 pKa = 11.84 VPGKK463 pKa = 9.62 EE464 pKa = 3.75 LSRR467 pKa = 11.84 ILDD470 pKa = 3.4 MDD472 pKa = 3.16 RR473 pKa = 11.84 WEE475 pKa = 4.94 CLSGMLSSNVTLRR488 pKa = 11.84 DD489 pKa = 3.44 LASVPGANGAEE500 pKa = 4.17 QIALVMVGAEE510 pKa = 4.21 LARR513 pKa = 11.84 DD514 pKa = 3.74 EE515 pKa = 4.73 GSSAVEE521 pKa = 4.18 LKK523 pKa = 10.78 RR524 pKa = 11.84 MYY526 pKa = 10.54 TSVSSKK532 pKa = 7.57 WARR535 pKa = 11.84 ASMRR539 pKa = 11.84 RR540 pKa = 11.84 DD541 pKa = 3.18 EE542 pKa = 4.27 WSDD545 pKa = 4.89 AIMQMLGEE553 pKa = 5.02 SIPTDD558 pKa = 2.99 IAIIIGRR565 pKa = 11.84 AAVNWYY571 pKa = 6.75 YY572 pKa = 10.86 TPGRR576 pKa = 11.84 KK577 pKa = 7.46 EE578 pKa = 3.94 TKK580 pKa = 8.06 EE581 pKa = 3.57 WSSIVDD587 pKa = 3.53 GCTRR591 pKa = 11.84 TVITTCLVRR600 pKa = 11.84 AEE602 pKa = 4.31 EE603 pKa = 4.06 DD604 pKa = 3.53 RR605 pKa = 11.84 EE606 pKa = 4.45 LEE608 pKa = 4.23 VSDD611 pKa = 5.15 GDD613 pKa = 4.39 IPPDD617 pKa = 3.76 VINAEE622 pKa = 3.86 AMTRR626 pKa = 11.84 LAVRR630 pKa = 11.84 MEE632 pKa = 4.56 LPQAMGGGWLKK643 pKa = 9.84 LTEE646 pKa = 4.23 DD647 pKa = 3.52 QRR649 pKa = 11.84 LTTLMLSEE657 pKa = 4.74 AKK659 pKa = 10.55 SPTLAAMEE667 pKa = 4.03 VVNFNNWMGRR677 pKa = 11.84 RR678 pKa = 11.84 RR679 pKa = 11.84 GVKK682 pKa = 8.45 VTMAAVARR690 pKa = 11.84 WIHH693 pKa = 4.66 ATRR696 pKa = 11.84 YY697 pKa = 7.92 MVYY700 pKa = 10.67 DD701 pKa = 3.81 GDD703 pKa = 4.83 LLVALAKK710 pKa = 10.25 EE711 pKa = 4.6 HH712 pKa = 6.02 FRR714 pKa = 11.84 QATGHH719 pKa = 6.17 SLTDD723 pKa = 3.0 EE724 pKa = 4.24 RR725 pKa = 11.84 KK726 pKa = 8.76 RR727 pKa = 11.84 YY728 pKa = 8.77 LVSYY732 pKa = 7.27 VHH734 pKa = 6.23 VRR736 pKa = 11.84 RR737 pKa = 11.84 SDD739 pKa = 3.4 GGIGLPDD746 pKa = 3.74 EE747 pKa = 4.76 NGHH750 pKa = 4.88 VYY752 pKa = 10.43 EE753 pKa = 5.4 RR754 pKa = 11.84 GTLGIRR760 pKa = 11.84 RR761 pKa = 11.84 WAQPDD766 pKa = 3.35 GRR768 pKa = 11.84 KK769 pKa = 9.94 SYY771 pKa = 11.63 VDD773 pKa = 3.45 DD774 pKa = 4.1 TCWDD778 pKa = 4.1 LKK780 pKa = 10.5 PFRR783 pKa = 11.84 LGQATKK789 pKa = 10.38 RR790 pKa = 11.84 KK791 pKa = 9.13 EE792 pKa = 4.11 KK793 pKa = 11.08 VKK795 pKa = 9.97 DD796 pKa = 3.53 TVRR799 pKa = 11.84 GLEE802 pKa = 3.82 KK803 pKa = 10.42 CDD805 pKa = 3.59 VYY807 pKa = 11.63 SEE809 pKa = 4.15 VLRR812 pKa = 11.84 AEE814 pKa = 4.35 GEE816 pKa = 4.02 RR817 pKa = 11.84 LEE819 pKa = 5.14 ALDD822 pKa = 3.72 ARR824 pKa = 11.84 IVVAAPLHH832 pKa = 5.11 VALMIRR838 pKa = 11.84 CMLKK842 pKa = 10.57 DD843 pKa = 3.25 VSKK846 pKa = 10.83 RR847 pKa = 11.84 GNEE850 pKa = 3.64 EE851 pKa = 3.72 AFRR854 pKa = 11.84 MIARR858 pKa = 11.84 ITRR861 pKa = 11.84 MASAVEE867 pKa = 4.34 HH868 pKa = 5.79 VPGMLADD875 pKa = 3.68 ILNAVGNYY883 pKa = 10.09 AIEE886 pKa = 4.04
Molecular weight: 100.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.029
IPC2_protein 7.468
IPC_protein 7.293
Toseland 7.029
ProMoST 7.878
Dawson 8.009
Bjellqvist 8.419
Wikipedia 7.922
Rodwell 8.039
Grimsley 7.102
Solomon 8.053
Lehninger 8.068
Nozaki 8.639
DTASelect 8.141
Thurlkill 8.2
EMBOSS 8.229
Sillero 8.521
Patrickios 4.609
IPC_peptide 8.053
IPC2_peptide 7.424
IPC2.peptide.svr19 7.426
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3825
843
1105
956.3
108.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.033 ± 0.411
1.15 ± 0.191
6.039 ± 0.2
7.477 ± 0.264
3.19 ± 0.651
6.588 ± 0.344
2.144 ± 0.196
4.967 ± 0.293
5.961 ± 0.313
8.863 ± 0.108
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.392 ± 0.355
3.608 ± 0.312
3.399 ± 0.461
3.163 ± 0.464
7.268 ± 0.576
7.268 ± 0.674
4.941 ± 0.304
7.346 ± 0.226
1.778 ± 0.188
3.425 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here