Capybara microvirus Cap3_SP_468
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8P5|A0A4P8W8P5_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_468 OX=2585463 PE=3 SV=1
MM1 pKa = 7.43 NKK3 pKa = 9.74 LFKK6 pKa = 9.96 PDD8 pKa = 3.47 EE9 pKa = 4.45 VGSYY13 pKa = 9.24 PVNVQSDD20 pKa = 3.41 FDD22 pKa = 3.37 QYY24 pKa = 10.6 YY25 pKa = 9.97 FEE27 pKa = 4.95 NEE29 pKa = 4.1 VVKK32 pKa = 10.87 KK33 pKa = 9.52 FVKK36 pKa = 10.02 TGVDD40 pKa = 3.38 NEE42 pKa = 4.29 GDD44 pKa = 3.53 DD45 pKa = 3.65 VGYY48 pKa = 9.94 IQEE51 pKa = 4.18 KK52 pKa = 9.99 VIVHH56 pKa = 5.98 KK57 pKa = 10.79 RR58 pKa = 11.84 LIKK61 pKa = 10.75 DD62 pKa = 3.4 VVAQDD67 pKa = 3.47 ADD69 pKa = 4.06 SVGVEE74 pKa = 4.6 PYY76 pKa = 10.68 LKK78 pKa = 10.23 MMALSGQSLPDD89 pKa = 3.15 VSFTDD94 pKa = 4.36 EE95 pKa = 4.68 INDD98 pKa = 3.42 FTQFPEE104 pKa = 4.25 DD105 pKa = 3.18 VGDD108 pKa = 4.39 AMRR111 pKa = 11.84 LGDD114 pKa = 4.59 KK115 pKa = 10.99 ANALYY120 pKa = 10.69 ASLPEE125 pKa = 4.3 EE126 pKa = 3.57 IRR128 pKa = 11.84 RR129 pKa = 11.84 NYY131 pKa = 10.02 TLDD134 pKa = 2.88 QFMQSDD140 pKa = 4.32 YY141 pKa = 11.23 EE142 pKa = 4.64 KK143 pKa = 10.58 IVSDD147 pKa = 3.55 YY148 pKa = 11.32 LALKK152 pKa = 9.63 KK153 pKa = 10.78 AEE155 pKa = 4.03 IEE157 pKa = 3.99 AAQKK161 pKa = 10.75 GGSEE165 pKa = 4.26 NVV167 pKa = 3.13
Molecular weight: 18.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.428
IPC2_protein 4.329
IPC_protein 4.291
Toseland 4.101
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.151
Rodwell 4.113
Grimsley 3.999
Solomon 4.253
Lehninger 4.202
Nozaki 4.355
DTASelect 4.571
Thurlkill 4.126
EMBOSS 4.164
Sillero 4.393
Patrickios 3.834
IPC_peptide 4.253
IPC2_peptide 4.38
IPC2.peptide.svr19 4.343
Protein with the highest isoelectric point:
>tr|A0A4V1FVU0|A0A4V1FVU0_9VIRU Prophage CP4-57 regulatory protein OS=Capybara microvirus Cap3_SP_468 OX=2585463 PE=4 SV=1
MM1 pKa = 7.75 TYY3 pKa = 7.39 MTKK6 pKa = 9.89 QQDD9 pKa = 3.4 HH10 pKa = 6.79 FIKK13 pKa = 10.03 YY14 pKa = 10.33 LKK16 pKa = 10.4 YY17 pKa = 10.52 SLVNVDD23 pKa = 4.09 SLEE26 pKa = 4.23 NDD28 pKa = 3.32 DD29 pKa = 4.79 FIYY32 pKa = 10.66 RR33 pKa = 11.84 IDD35 pKa = 3.58 VVVRR39 pKa = 11.84 KK40 pKa = 8.12 TGISRR45 pKa = 11.84 RR46 pKa = 11.84 TIYY49 pKa = 10.31 RR50 pKa = 11.84 LCKK53 pKa = 10.11 EE54 pKa = 4.08 YY55 pKa = 10.94 DD56 pKa = 3.26 FPIYY60 pKa = 10.16 RR61 pKa = 11.84 GCFTVNLARR70 pKa = 11.84 FWHH73 pKa = 5.57 VFGTNKK79 pKa = 10.25 RR80 pKa = 11.84 STKK83 pKa = 10.16 KK84 pKa = 9.99 INLFNTEE91 pKa = 3.68 YY92 pKa = 10.57 RR93 pKa = 11.84 IEE95 pKa = 4.03 EE96 pKa = 4.34 DD97 pKa = 2.89 KK98 pKa = 11.71 EE99 pKa = 3.86 EE100 pKa = 4.26 IYY102 pKa = 10.68 RR103 pKa = 11.84 IAHH106 pKa = 5.0 VNWIGVHH113 pKa = 5.41
Molecular weight: 13.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.705
IPC2_protein 8.697
IPC_protein 8.697
Toseland 9.063
ProMoST 8.99
Dawson 9.399
Bjellqvist 9.253
Wikipedia 9.619
Rodwell 9.516
Grimsley 9.487
Solomon 9.502
Lehninger 9.458
Nozaki 9.238
DTASelect 9.18
Thurlkill 9.238
EMBOSS 9.516
Sillero 9.399
Patrickios 4.38
IPC_peptide 9.487
IPC2_peptide 8.039
IPC2.peptide.svr19 7.745
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1494
98
535
213.4
24.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.89 ± 0.67
1.339 ± 0.682
6.426 ± 0.695
4.819 ± 0.519
5.154 ± 0.443
6.225 ± 0.624
2.008 ± 0.369
5.957 ± 0.638
7.296 ± 1.029
9.17 ± 0.944
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.075 ± 0.268
5.288 ± 0.318
4.15 ± 0.786
4.351 ± 0.545
3.681 ± 0.764
8.099 ± 0.946
5.087 ± 0.74
6.627 ± 0.854
1.071 ± 0.216
5.288 ± 0.632
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here