Erwinia phage vB_EamM_Asesino

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Erskinevirus; Erwinia virus Asesino

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2I9Z5|A0A1B2I9Z5_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Asesino OX=1883370 GN=ASESINO_86 PE=4 SV=1
MM1 pKa = 7.57IDD3 pKa = 3.54IYY5 pKa = 10.96TDD7 pKa = 3.37YY8 pKa = 11.55AAVLTVNRR16 pKa = 11.84HH17 pKa = 4.88EE18 pKa = 5.16DD19 pKa = 3.08RR20 pKa = 11.84AAPMLDD26 pKa = 3.7LVTLGIDD33 pKa = 3.21YY34 pKa = 10.23GYY36 pKa = 11.05DD37 pKa = 3.14VALSDD42 pKa = 4.81VYY44 pKa = 10.72FEE46 pKa = 4.98PSADD50 pKa = 3.74PADD53 pKa = 3.55EE54 pKa = 4.47TIRR57 pKa = 11.84LEE59 pKa = 4.49GIIVKK64 pKa = 10.21CAVALGNRR72 pKa = 11.84LGVALNPQIVYY83 pKa = 9.2QKK85 pKa = 9.79PKK87 pKa = 7.49EE88 pKa = 4.29TVRR91 pKa = 11.84ILHH94 pKa = 6.25GLLEE98 pKa = 4.32TFEE101 pKa = 5.81DD102 pKa = 4.81FDD104 pKa = 6.94DD105 pKa = 4.35SDD107 pKa = 3.84SLYY110 pKa = 10.66GIVMSGEE117 pKa = 3.98TPEE120 pKa = 5.32FILEE124 pKa = 4.05NMCRR128 pKa = 11.84LVYY131 pKa = 10.75GDD133 pKa = 4.32NNLHH137 pKa = 6.63FEE139 pKa = 4.22DD140 pKa = 5.32LIVRR144 pKa = 11.84VGPRR148 pKa = 11.84VMTVMRR154 pKa = 11.84NYY156 pKa = 10.32LAASALDD163 pKa = 3.87EE164 pKa = 4.27QKK166 pKa = 11.39GDD168 pKa = 3.38NDD170 pKa = 3.48YY171 pKa = 11.63LRR173 pKa = 11.84IGRR176 pKa = 11.84IAAYY180 pKa = 10.42LRR182 pKa = 11.84VFPQNPSAFVFLNLPQEE199 pKa = 4.33PDD201 pKa = 3.43LQVVQQSFTFDD212 pKa = 3.17EE213 pKa = 4.35EE214 pKa = 4.63EE215 pKa = 4.29YY216 pKa = 10.76PEE218 pKa = 5.88DD219 pKa = 4.31EE220 pKa = 4.58LLLMYY225 pKa = 10.59SVGLSVIPADD235 pKa = 3.91TYY237 pKa = 10.76EE238 pKa = 4.31DD239 pKa = 4.01AYY241 pKa = 10.99AALAGNLEE249 pKa = 4.14KK250 pKa = 11.02LNTDD254 pKa = 4.5GYY256 pKa = 9.0PALPILEE263 pKa = 4.27QGMAALKK270 pKa = 9.58TIYY273 pKa = 10.48GHH275 pKa = 7.4DD276 pKa = 3.27NVTEE280 pKa = 4.06EE281 pKa = 4.25EE282 pKa = 4.48NYY284 pKa = 10.46EE285 pKa = 3.83QDD287 pKa = 3.06

Molecular weight: 32.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2IA83|A0A1B2IA83_9CAUD Site-specific DNA-methyltransferase (adenine-specific) OS=Erwinia phage vB_EamM_Asesino OX=1883370 GN=ASESINO_121 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.28FFFVVVVIFCIGAVLRR18 pKa = 11.84SFLRR22 pKa = 11.84YY23 pKa = 8.99LRR25 pKa = 11.84HH26 pKa = 5.9RR27 pKa = 11.84QRR29 pKa = 11.84AHH31 pKa = 7.79LAQQLNAYY39 pKa = 8.61RR40 pKa = 11.84QVSEE44 pKa = 4.1YY45 pKa = 10.33VDD47 pKa = 3.05RR48 pKa = 11.84AGRR51 pKa = 11.84EE52 pKa = 3.42KK53 pKa = 10.62WMMVICFLIGLWCTIKK69 pKa = 10.85LFNN72 pKa = 5.02

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

77544

22

2379

279.9

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.993 ± 0.19

0.914 ± 0.064

6.387 ± 0.106

6.141 ± 0.156

4.042 ± 0.074

6.768 ± 0.195

2.062 ± 0.097

5.326 ± 0.103

5.384 ± 0.135

9.004 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.067

4.897 ± 0.091

4.435 ± 0.089

3.76 ± 0.086

5.441 ± 0.128

5.678 ± 0.105

6.413 ± 0.146

7.2 ± 0.111

1.515 ± 0.054

3.887 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski