Garlic virus B

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus; Acarallexivirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A0YT54|A0A0A0YT54_9VIRU NABP OS=Garlic virus B OX=12432 GN=ORFVI PE=4 SV=1
MM1 pKa = 6.78TTVQKK6 pKa = 11.0LLDD9 pKa = 4.13QISDD13 pKa = 3.73PNARR17 pKa = 11.84AAYY20 pKa = 10.16SQDD23 pKa = 3.31CLSAAKK29 pKa = 9.9HH30 pKa = 5.48AATKK34 pKa = 11.0AMTIAPYY41 pKa = 10.82AVTQTEE47 pKa = 4.29ALTLEE52 pKa = 4.81RR53 pKa = 11.84IGITTSPFATTSHH66 pKa = 4.89THH68 pKa = 5.93AADD71 pKa = 4.1KK72 pKa = 10.63IIEE75 pKa = 4.28NDD77 pKa = 3.51CLDD80 pKa = 3.67IIGRR84 pKa = 11.84YY85 pKa = 8.49LPKK88 pKa = 10.75DD89 pKa = 3.58SVTLIQLKK97 pKa = 9.76RR98 pKa = 11.84AKK100 pKa = 10.29LHH102 pKa = 5.43LLKK105 pKa = 10.54RR106 pKa = 11.84GPNTDD111 pKa = 2.59VFRR114 pKa = 11.84NYY116 pKa = 9.95CHH118 pKa = 6.47EE119 pKa = 4.61PKK121 pKa = 10.48DD122 pKa = 3.63VLRR125 pKa = 11.84FGLTNPHH132 pKa = 6.31SCPVVDD138 pKa = 4.84TPCAVISDD146 pKa = 4.24TLHH149 pKa = 6.31FMSSQQVWTLFEE161 pKa = 4.8RR162 pKa = 11.84NPRR165 pKa = 11.84LQKK168 pKa = 10.92LYY170 pKa = 9.16GTLVLPIEE178 pKa = 4.97ALNKK182 pKa = 10.2LPSLFPEE189 pKa = 4.6IYY191 pKa = 10.16KK192 pKa = 10.47LEE194 pKa = 4.24YY195 pKa = 9.54YY196 pKa = 8.87EE197 pKa = 4.51KK198 pKa = 10.27HH199 pKa = 5.41FAYY202 pKa = 9.95MPGGHH207 pKa = 6.74GGGAYY212 pKa = 8.55IHH214 pKa = 7.06SYY216 pKa = 8.85GTLKK220 pKa = 10.11WLNTAQIGTEE230 pKa = 4.19GNHH233 pKa = 6.23LSIEE237 pKa = 4.27KK238 pKa = 10.18IEE240 pKa = 4.33TKK242 pKa = 10.11AAHH245 pKa = 6.64HH246 pKa = 6.39IVLIQRR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84PQLSWPLPPLWVFHH268 pKa = 6.67ASDD271 pKa = 4.11YY272 pKa = 11.35VKK274 pKa = 10.55LPEE277 pKa = 3.91IFYY280 pKa = 10.05PSEE283 pKa = 4.11ANVQKK288 pKa = 10.63AYY290 pKa = 10.66SHH292 pKa = 6.05VFIKK296 pKa = 10.56RR297 pKa = 11.84LQLYY301 pKa = 8.31CFSVKK306 pKa = 10.2AVSLRR311 pKa = 11.84DD312 pKa = 3.18IFAKK316 pKa = 10.0IRR318 pKa = 11.84QVLPTEE324 pKa = 3.95EE325 pKa = 4.4LSRR328 pKa = 11.84FSTADD333 pKa = 4.48LIRR336 pKa = 11.84LANYY340 pKa = 10.44LLFITGMQQTSDD352 pKa = 3.74YY353 pKa = 10.41QSPLMEE359 pKa = 4.39SLFGKK364 pKa = 8.85MCLSIRR370 pKa = 11.84ARR372 pKa = 11.84VRR374 pKa = 11.84EE375 pKa = 3.85FLQNLMGKK383 pKa = 9.57SSYY386 pKa = 10.93AALLTVTDD394 pKa = 3.49VRR396 pKa = 11.84PVYY399 pKa = 9.33FTTKK403 pKa = 8.71PVRR406 pKa = 11.84RR407 pKa = 11.84AICGHH412 pKa = 5.05QWFEE416 pKa = 4.54DD417 pKa = 3.9EE418 pKa = 5.2EE419 pKa = 5.33KK420 pKa = 11.03DD421 pKa = 4.04EE422 pKa = 4.45EE423 pKa = 4.65TGTTPSITGYY433 pKa = 9.8PSPSPDD439 pKa = 4.61DD440 pKa = 4.53LDD442 pKa = 4.18LLAEE446 pKa = 4.41FEE448 pKa = 4.28RR449 pKa = 11.84HH450 pKa = 4.26EE451 pKa = 4.19QANEE455 pKa = 3.57EE456 pKa = 4.17RR457 pKa = 11.84NQPSSEE463 pKa = 4.05NPNPKK468 pKa = 9.16QQSTAHH474 pKa = 5.99SSAEE478 pKa = 3.72RR479 pKa = 11.84APNGKK484 pKa = 8.09FTNVGGRR491 pKa = 11.84APEE494 pKa = 4.47SVHH497 pKa = 5.47EE498 pKa = 4.51TQDD501 pKa = 2.95HH502 pKa = 5.43PQQVKK507 pKa = 10.26EE508 pKa = 4.21GNDD511 pKa = 3.48DD512 pKa = 3.73NMVDD516 pKa = 4.6GQFTDD521 pKa = 4.5CTDD524 pKa = 3.6SDD526 pKa = 4.78CDD528 pKa = 3.7QSDD531 pKa = 3.87NEE533 pKa = 4.34SDD535 pKa = 3.75SDD537 pKa = 4.35SEE539 pKa = 4.37CQEE542 pKa = 4.14SQQLQDD548 pKa = 5.17DD549 pKa = 5.02LLPDD553 pKa = 4.08EE554 pKa = 6.22DD555 pKa = 5.55DD556 pKa = 4.21LPEE559 pKa = 5.31LEE561 pKa = 4.66SVPGEE566 pKa = 4.09LDD568 pKa = 3.43TEE570 pKa = 4.27NPEE573 pKa = 3.94AALEE577 pKa = 4.08SDD579 pKa = 3.67EE580 pKa = 4.68TEE582 pKa = 4.09PTAKK586 pKa = 10.76AEE588 pKa = 4.48DD589 pKa = 4.5DD590 pKa = 4.03DD591 pKa = 6.44DD592 pKa = 6.06GEE594 pKa = 4.45SSEE597 pKa = 5.05DD598 pKa = 3.57AQTSSSKK605 pKa = 10.98PEE607 pKa = 3.97TTPGVALEE615 pKa = 4.43PLDD618 pKa = 5.82DD619 pKa = 3.86EE620 pKa = 5.28TIDD623 pKa = 3.33ILKK626 pKa = 8.68MHH628 pKa = 7.14GFTNYY633 pKa = 9.72KK634 pKa = 8.33PQHH637 pKa = 6.48DD638 pKa = 5.02GEE640 pKa = 4.36FQIPPVFYY648 pKa = 10.52NRR650 pKa = 11.84QARR653 pKa = 11.84FLPLHH658 pKa = 6.09EE659 pKa = 5.72GIVNTAQSRR668 pKa = 11.84FLDD671 pKa = 3.36MAQALKK677 pKa = 10.47RR678 pKa = 11.84NCYY681 pKa = 9.86NYY683 pKa = 10.77RR684 pKa = 11.84LDD686 pKa = 4.06AKK688 pKa = 10.41RR689 pKa = 11.84ATAFMSDD696 pKa = 3.27VKK698 pKa = 11.38NNLTGLVLPKK708 pKa = 10.11LDD710 pKa = 4.63RR711 pKa = 11.84DD712 pKa = 4.2LLTSWVALAEE722 pKa = 4.06NAKK725 pKa = 10.44RR726 pKa = 11.84NVGLIVIHH734 pKa = 6.23GAGGAGKK741 pKa = 10.07SRR743 pKa = 11.84ALQEE747 pKa = 3.88LMRR750 pKa = 11.84NGLVDD755 pKa = 3.68TNEE758 pKa = 3.77VNIIVPTINLAVDD771 pKa = 3.89WKK773 pKa = 10.83KK774 pKa = 10.85KK775 pKa = 10.34LPSVDD780 pKa = 3.16PRR782 pKa = 11.84RR783 pKa = 11.84IMTFEE788 pKa = 4.01KK789 pKa = 10.17ACEE792 pKa = 4.0RR793 pKa = 11.84EE794 pKa = 4.35GKK796 pKa = 10.43SIVIMDD802 pKa = 5.07DD803 pKa = 3.38YY804 pKa = 11.82GKK806 pKa = 10.9LPAGYY811 pKa = 9.92VDD813 pKa = 5.05AYY815 pKa = 11.06LAIKK819 pKa = 10.03FNVEE823 pKa = 3.93MVILTGDD830 pKa = 3.16QRR832 pKa = 11.84QSVFHH837 pKa = 6.77NDD839 pKa = 3.2KK840 pKa = 10.53KK841 pKa = 10.9DD842 pKa = 3.48SQIALLSSNTDD853 pKa = 2.75HH854 pKa = 7.54FKK856 pKa = 10.69RR857 pKa = 11.84YY858 pKa = 9.34CDD860 pKa = 3.6YY861 pKa = 11.17YY862 pKa = 11.6LNATHH867 pKa = 6.81RR868 pKa = 11.84QPRR871 pKa = 11.84RR872 pKa = 11.84LANPIKK878 pKa = 10.05VHH880 pKa = 6.27AEE882 pKa = 3.92RR883 pKa = 11.84EE884 pKa = 3.73IGGAIKK890 pKa = 10.18HH891 pKa = 5.68ATLIPTNAMTLVPAFRR907 pKa = 11.84SQSLLTDD914 pKa = 4.09LGRR917 pKa = 11.84QAMTYY922 pKa = 10.18AGCQGLTLPHH932 pKa = 6.01LTIVLDD938 pKa = 4.36KK939 pKa = 10.04DD940 pKa = 3.68TPLCSDD946 pKa = 3.5EE947 pKa = 4.38VLYY950 pKa = 9.62TALSRR955 pKa = 11.84ASEE958 pKa = 4.34TITFVNTHH966 pKa = 5.83SNNKK970 pKa = 9.8DD971 pKa = 3.22FLAKK975 pKa = 10.18LDD977 pKa = 3.67STPYY981 pKa = 10.73LKK983 pKa = 9.81TLISGVRR990 pKa = 11.84EE991 pKa = 3.98DD992 pKa = 3.92EE993 pKa = 4.12QAGRR997 pKa = 11.84DD998 pKa = 3.76NAPPEE1003 pKa = 4.67PIVRR1007 pKa = 11.84DD1008 pKa = 4.13GPTKK1012 pKa = 9.14THH1014 pKa = 6.48IPVANDD1020 pKa = 4.44KK1021 pKa = 10.74IQLDD1025 pKa = 4.04DD1026 pKa = 5.17KK1027 pKa = 11.23IEE1029 pKa = 4.06AMEE1032 pKa = 4.97DD1033 pKa = 3.05KK1034 pKa = 9.95DD1035 pKa = 4.96TRR1037 pKa = 11.84EE1038 pKa = 3.87LWSGEE1043 pKa = 3.84EE1044 pKa = 4.18KK1045 pKa = 10.4TNLMQTQDD1053 pKa = 3.35PTIQLFPHH1061 pKa = 5.54QQAKK1065 pKa = 10.89DD1066 pKa = 3.24EE1067 pKa = 4.47ALFKK1071 pKa = 10.13ITINEE1076 pKa = 4.65RR1077 pKa = 11.84IRR1079 pKa = 11.84LAAPEE1084 pKa = 4.22TNRR1087 pKa = 11.84KK1088 pKa = 8.48ALKK1091 pKa = 9.27DD1092 pKa = 3.69TINAGDD1098 pKa = 4.5LLFEE1102 pKa = 5.19AYY1104 pKa = 10.18ASFMNVPRR1112 pKa = 11.84EE1113 pKa = 3.92TQPFDD1118 pKa = 3.22KK1119 pKa = 10.56RR1120 pKa = 11.84LWAHH1124 pKa = 6.37CRR1126 pKa = 11.84QLALRR1131 pKa = 11.84TYY1133 pKa = 10.57LSKK1136 pKa = 9.68PTSNLQQGAQRR1147 pKa = 11.84QDD1149 pKa = 2.93PDD1151 pKa = 3.87FPDD1154 pKa = 3.11NAIALFNKK1162 pKa = 8.34SQWVKK1167 pKa = 10.35KK1168 pKa = 10.25LEE1170 pKa = 4.06KK1171 pKa = 10.47VGCKK1175 pKa = 9.95FKK1177 pKa = 11.02AGQTISAFKK1186 pKa = 10.55QSTVLLTTTMALYY1199 pKa = 10.13LRR1201 pKa = 11.84KK1202 pKa = 9.51KK1203 pKa = 10.63RR1204 pKa = 11.84EE1205 pKa = 3.53GHH1207 pKa = 5.43QPDD1210 pKa = 3.93NVFVMCEE1217 pKa = 3.73KK1218 pKa = 10.34TPEE1221 pKa = 4.02QFNSFVLTKK1230 pKa = 9.95WDD1232 pKa = 4.4FSRR1235 pKa = 11.84PNYY1238 pKa = 9.07TSDD1241 pKa = 3.01YY1242 pKa = 8.03TQYY1245 pKa = 11.34DD1246 pKa = 3.52QSQDD1250 pKa = 3.34SAFLNFEE1257 pKa = 4.11LRR1259 pKa = 11.84KK1260 pKa = 9.72ARR1262 pKa = 11.84HH1263 pKa = 5.95FGLPDD1268 pKa = 3.43EE1269 pKa = 5.15VVDD1272 pKa = 5.71FYY1274 pKa = 12.08AFVKK1278 pKa = 8.76THH1280 pKa = 6.55AKK1282 pKa = 9.24TFLGNLAVMRR1292 pKa = 11.84LSGEE1296 pKa = 4.14GPTFDD1301 pKa = 6.1ANTEE1305 pKa = 4.05CNIAYY1310 pKa = 8.34DD1311 pKa = 3.78ALRR1314 pKa = 11.84FQLDD1318 pKa = 3.62PSVNACYY1325 pKa = 10.33AGDD1328 pKa = 3.77DD1329 pKa = 3.85LVRR1332 pKa = 11.84DD1333 pKa = 4.11KK1334 pKa = 11.59ACDD1337 pKa = 3.47EE1338 pKa = 4.26RR1339 pKa = 11.84PGWKK1343 pKa = 9.91YY1344 pKa = 11.05SEE1346 pKa = 4.37PLFSLKK1352 pKa = 10.54AKK1354 pKa = 10.13PLVTNKK1360 pKa = 9.9PDD1362 pKa = 3.19FCGWRR1367 pKa = 11.84LTKK1370 pKa = 10.71YY1371 pKa = 10.53GIVKK1375 pKa = 10.45SPIQLYY1381 pKa = 10.26QSLQLALRR1389 pKa = 11.84LGKK1392 pKa = 10.19VEE1394 pKa = 4.03EE1395 pKa = 4.42VKK1397 pKa = 10.49RR1398 pKa = 11.84SYY1400 pKa = 11.23AIDD1403 pKa = 3.38YY1404 pKa = 9.93LFAYY1408 pKa = 10.43RR1409 pKa = 11.84LGDD1412 pKa = 3.38QLYY1415 pKa = 10.56DD1416 pKa = 3.39IFDD1419 pKa = 3.81EE1420 pKa = 5.39DD1421 pKa = 4.24EE1422 pKa = 4.2LAKK1425 pKa = 10.5HH1426 pKa = 5.31QLVTRR1431 pKa = 11.84TLIKK1435 pKa = 10.51KK1436 pKa = 9.59GMQPPSSGEE1445 pKa = 3.8HH1446 pKa = 6.19LPTFHH1451 pKa = 6.1VTSDD1455 pKa = 3.91RR1456 pKa = 11.84LIQDD1460 pKa = 3.2PRR1462 pKa = 11.84ATKK1465 pKa = 10.55VSTFEE1470 pKa = 3.82SDD1472 pKa = 4.03RR1473 pKa = 11.84VILPLEE1479 pKa = 4.65IIDD1482 pKa = 4.01DD1483 pKa = 4.05HH1484 pKa = 6.68FAFASKK1490 pKa = 10.56SDD1492 pKa = 3.86RR1493 pKa = 11.84NDD1495 pKa = 2.59MTTASNQQTSEE1506 pKa = 4.56SNVSANQTDD1515 pKa = 3.54YY1516 pKa = 11.81GGTLLTDD1523 pKa = 3.97MFPDD1527 pKa = 3.27IAMLL1531 pKa = 3.92

Molecular weight:
172.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A0YR04|A0A0A0YR04_9VIRU 40kDa protein OS=Garlic virus B OX=12432 GN=ORFIV PE=4 SV=1
MM1 pKa = 7.54SFTPPPDD8 pKa = 3.31NSKK11 pKa = 11.04SILAIAVGAAAALIVFTIRR30 pKa = 11.84TNHH33 pKa = 5.91SASVGDD39 pKa = 3.63NTHH42 pKa = 6.91RR43 pKa = 11.84LPYY46 pKa = 9.75GGQYY50 pKa = 10.1RR51 pKa = 11.84DD52 pKa = 3.33GNKK55 pKa = 9.61LVNYY59 pKa = 8.82NGPHH63 pKa = 6.3TGASPPSILWPALSAIILTLVIHH86 pKa = 6.29FRR88 pKa = 11.84SGTRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84ICIHH98 pKa = 6.25CSEE101 pKa = 4.7STSAHH106 pKa = 4.7QSS108 pKa = 3.06

Molecular weight:
11.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2610

108

1531

435.0

48.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.548 ± 0.553

1.686 ± 0.274

6.513 ± 0.884

5.556 ± 0.492

4.023 ± 0.512

4.828 ± 0.553

3.18 ± 0.602

5.134 ± 0.623

5.556 ± 0.49

10.345 ± 0.898

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.648 ± 0.352

5.019 ± 0.533

5.862 ± 0.419

4.866 ± 0.456

5.287 ± 0.24

7.05 ± 0.574

7.625 ± 0.602

4.674 ± 0.422

0.69 ± 0.156

2.912 ± 0.317

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski