Garlic virus B
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0YT54|A0A0A0YT54_9VIRU NABP OS=Garlic virus B OX=12432 GN=ORFVI PE=4 SV=1
MM1 pKa = 6.78 TTVQKK6 pKa = 11.0 LLDD9 pKa = 4.13 QISDD13 pKa = 3.73 PNARR17 pKa = 11.84 AAYY20 pKa = 10.16 SQDD23 pKa = 3.31 CLSAAKK29 pKa = 9.9 HH30 pKa = 5.48 AATKK34 pKa = 11.0 AMTIAPYY41 pKa = 10.82 AVTQTEE47 pKa = 4.29 ALTLEE52 pKa = 4.81 RR53 pKa = 11.84 IGITTSPFATTSHH66 pKa = 4.89 THH68 pKa = 5.93 AADD71 pKa = 4.1 KK72 pKa = 10.63 IIEE75 pKa = 4.28 NDD77 pKa = 3.51 CLDD80 pKa = 3.67 IIGRR84 pKa = 11.84 YY85 pKa = 8.49 LPKK88 pKa = 10.75 DD89 pKa = 3.58 SVTLIQLKK97 pKa = 9.76 RR98 pKa = 11.84 AKK100 pKa = 10.29 LHH102 pKa = 5.43 LLKK105 pKa = 10.54 RR106 pKa = 11.84 GPNTDD111 pKa = 2.59 VFRR114 pKa = 11.84 NYY116 pKa = 9.95 CHH118 pKa = 6.47 EE119 pKa = 4.61 PKK121 pKa = 10.48 DD122 pKa = 3.63 VLRR125 pKa = 11.84 FGLTNPHH132 pKa = 6.31 SCPVVDD138 pKa = 4.84 TPCAVISDD146 pKa = 4.24 TLHH149 pKa = 6.31 FMSSQQVWTLFEE161 pKa = 4.8 RR162 pKa = 11.84 NPRR165 pKa = 11.84 LQKK168 pKa = 10.92 LYY170 pKa = 9.16 GTLVLPIEE178 pKa = 4.97 ALNKK182 pKa = 10.2 LPSLFPEE189 pKa = 4.6 IYY191 pKa = 10.16 KK192 pKa = 10.47 LEE194 pKa = 4.24 YY195 pKa = 9.54 YY196 pKa = 8.87 EE197 pKa = 4.51 KK198 pKa = 10.27 HH199 pKa = 5.41 FAYY202 pKa = 9.95 MPGGHH207 pKa = 6.74 GGGAYY212 pKa = 8.55 IHH214 pKa = 7.06 SYY216 pKa = 8.85 GTLKK220 pKa = 10.11 WLNTAQIGTEE230 pKa = 4.19 GNHH233 pKa = 6.23 LSIEE237 pKa = 4.27 KK238 pKa = 10.18 IEE240 pKa = 4.33 TKK242 pKa = 10.11 AAHH245 pKa = 6.64 HH246 pKa = 6.39 IVLIQRR252 pKa = 11.84 RR253 pKa = 11.84 RR254 pKa = 11.84 PQLSWPLPPLWVFHH268 pKa = 6.67 ASDD271 pKa = 4.11 YY272 pKa = 11.35 VKK274 pKa = 10.55 LPEE277 pKa = 3.91 IFYY280 pKa = 10.05 PSEE283 pKa = 4.11 ANVQKK288 pKa = 10.63 AYY290 pKa = 10.66 SHH292 pKa = 6.05 VFIKK296 pKa = 10.56 RR297 pKa = 11.84 LQLYY301 pKa = 8.31 CFSVKK306 pKa = 10.2 AVSLRR311 pKa = 11.84 DD312 pKa = 3.18 IFAKK316 pKa = 10.0 IRR318 pKa = 11.84 QVLPTEE324 pKa = 3.95 EE325 pKa = 4.4 LSRR328 pKa = 11.84 FSTADD333 pKa = 4.48 LIRR336 pKa = 11.84 LANYY340 pKa = 10.44 LLFITGMQQTSDD352 pKa = 3.74 YY353 pKa = 10.41 QSPLMEE359 pKa = 4.39 SLFGKK364 pKa = 8.85 MCLSIRR370 pKa = 11.84 ARR372 pKa = 11.84 VRR374 pKa = 11.84 EE375 pKa = 3.85 FLQNLMGKK383 pKa = 9.57 SSYY386 pKa = 10.93 AALLTVTDD394 pKa = 3.49 VRR396 pKa = 11.84 PVYY399 pKa = 9.33 FTTKK403 pKa = 8.71 PVRR406 pKa = 11.84 RR407 pKa = 11.84 AICGHH412 pKa = 5.05 QWFEE416 pKa = 4.54 DD417 pKa = 3.9 EE418 pKa = 5.2 EE419 pKa = 5.33 KK420 pKa = 11.03 DD421 pKa = 4.04 EE422 pKa = 4.45 EE423 pKa = 4.65 TGTTPSITGYY433 pKa = 9.8 PSPSPDD439 pKa = 4.61 DD440 pKa = 4.53 LDD442 pKa = 4.18 LLAEE446 pKa = 4.41 FEE448 pKa = 4.28 RR449 pKa = 11.84 HH450 pKa = 4.26 EE451 pKa = 4.19 QANEE455 pKa = 3.57 EE456 pKa = 4.17 RR457 pKa = 11.84 NQPSSEE463 pKa = 4.05 NPNPKK468 pKa = 9.16 QQSTAHH474 pKa = 5.99 SSAEE478 pKa = 3.72 RR479 pKa = 11.84 APNGKK484 pKa = 8.09 FTNVGGRR491 pKa = 11.84 APEE494 pKa = 4.47 SVHH497 pKa = 5.47 EE498 pKa = 4.51 TQDD501 pKa = 2.95 HH502 pKa = 5.43 PQQVKK507 pKa = 10.26 EE508 pKa = 4.21 GNDD511 pKa = 3.48 DD512 pKa = 3.73 NMVDD516 pKa = 4.6 GQFTDD521 pKa = 4.5 CTDD524 pKa = 3.6 SDD526 pKa = 4.78 CDD528 pKa = 3.7 QSDD531 pKa = 3.87 NEE533 pKa = 4.34 SDD535 pKa = 3.75 SDD537 pKa = 4.35 SEE539 pKa = 4.37 CQEE542 pKa = 4.14 SQQLQDD548 pKa = 5.17 DD549 pKa = 5.02 LLPDD553 pKa = 4.08 EE554 pKa = 6.22 DD555 pKa = 5.55 DD556 pKa = 4.21 LPEE559 pKa = 5.31 LEE561 pKa = 4.66 SVPGEE566 pKa = 4.09 LDD568 pKa = 3.43 TEE570 pKa = 4.27 NPEE573 pKa = 3.94 AALEE577 pKa = 4.08 SDD579 pKa = 3.67 EE580 pKa = 4.68 TEE582 pKa = 4.09 PTAKK586 pKa = 10.76 AEE588 pKa = 4.48 DD589 pKa = 4.5 DD590 pKa = 4.03 DD591 pKa = 6.44 DD592 pKa = 6.06 GEE594 pKa = 4.45 SSEE597 pKa = 5.05 DD598 pKa = 3.57 AQTSSSKK605 pKa = 10.98 PEE607 pKa = 3.97 TTPGVALEE615 pKa = 4.43 PLDD618 pKa = 5.82 DD619 pKa = 3.86 EE620 pKa = 5.28 TIDD623 pKa = 3.33 ILKK626 pKa = 8.68 MHH628 pKa = 7.14 GFTNYY633 pKa = 9.72 KK634 pKa = 8.33 PQHH637 pKa = 6.48 DD638 pKa = 5.02 GEE640 pKa = 4.36 FQIPPVFYY648 pKa = 10.52 NRR650 pKa = 11.84 QARR653 pKa = 11.84 FLPLHH658 pKa = 6.09 EE659 pKa = 5.72 GIVNTAQSRR668 pKa = 11.84 FLDD671 pKa = 3.36 MAQALKK677 pKa = 10.47 RR678 pKa = 11.84 NCYY681 pKa = 9.86 NYY683 pKa = 10.77 RR684 pKa = 11.84 LDD686 pKa = 4.06 AKK688 pKa = 10.41 RR689 pKa = 11.84 ATAFMSDD696 pKa = 3.27 VKK698 pKa = 11.38 NNLTGLVLPKK708 pKa = 10.11 LDD710 pKa = 4.63 RR711 pKa = 11.84 DD712 pKa = 4.2 LLTSWVALAEE722 pKa = 4.06 NAKK725 pKa = 10.44 RR726 pKa = 11.84 NVGLIVIHH734 pKa = 6.23 GAGGAGKK741 pKa = 10.07 SRR743 pKa = 11.84 ALQEE747 pKa = 3.88 LMRR750 pKa = 11.84 NGLVDD755 pKa = 3.68 TNEE758 pKa = 3.77 VNIIVPTINLAVDD771 pKa = 3.89 WKK773 pKa = 10.83 KK774 pKa = 10.85 KK775 pKa = 10.34 LPSVDD780 pKa = 3.16 PRR782 pKa = 11.84 RR783 pKa = 11.84 IMTFEE788 pKa = 4.01 KK789 pKa = 10.17 ACEE792 pKa = 4.0 RR793 pKa = 11.84 EE794 pKa = 4.35 GKK796 pKa = 10.43 SIVIMDD802 pKa = 5.07 DD803 pKa = 3.38 YY804 pKa = 11.82 GKK806 pKa = 10.9 LPAGYY811 pKa = 9.92 VDD813 pKa = 5.05 AYY815 pKa = 11.06 LAIKK819 pKa = 10.03 FNVEE823 pKa = 3.93 MVILTGDD830 pKa = 3.16 QRR832 pKa = 11.84 QSVFHH837 pKa = 6.77 NDD839 pKa = 3.2 KK840 pKa = 10.53 KK841 pKa = 10.9 DD842 pKa = 3.48 SQIALLSSNTDD853 pKa = 2.75 HH854 pKa = 7.54 FKK856 pKa = 10.69 RR857 pKa = 11.84 YY858 pKa = 9.34 CDD860 pKa = 3.6 YY861 pKa = 11.17 YY862 pKa = 11.6 LNATHH867 pKa = 6.81 RR868 pKa = 11.84 QPRR871 pKa = 11.84 RR872 pKa = 11.84 LANPIKK878 pKa = 10.05 VHH880 pKa = 6.27 AEE882 pKa = 3.92 RR883 pKa = 11.84 EE884 pKa = 3.73 IGGAIKK890 pKa = 10.18 HH891 pKa = 5.68 ATLIPTNAMTLVPAFRR907 pKa = 11.84 SQSLLTDD914 pKa = 4.09 LGRR917 pKa = 11.84 QAMTYY922 pKa = 10.18 AGCQGLTLPHH932 pKa = 6.01 LTIVLDD938 pKa = 4.36 KK939 pKa = 10.04 DD940 pKa = 3.68 TPLCSDD946 pKa = 3.5 EE947 pKa = 4.38 VLYY950 pKa = 9.62 TALSRR955 pKa = 11.84 ASEE958 pKa = 4.34 TITFVNTHH966 pKa = 5.83 SNNKK970 pKa = 9.8 DD971 pKa = 3.22 FLAKK975 pKa = 10.18 LDD977 pKa = 3.67 STPYY981 pKa = 10.73 LKK983 pKa = 9.81 TLISGVRR990 pKa = 11.84 EE991 pKa = 3.98 DD992 pKa = 3.92 EE993 pKa = 4.12 QAGRR997 pKa = 11.84 DD998 pKa = 3.76 NAPPEE1003 pKa = 4.67 PIVRR1007 pKa = 11.84 DD1008 pKa = 4.13 GPTKK1012 pKa = 9.14 THH1014 pKa = 6.48 IPVANDD1020 pKa = 4.44 KK1021 pKa = 10.74 IQLDD1025 pKa = 4.04 DD1026 pKa = 5.17 KK1027 pKa = 11.23 IEE1029 pKa = 4.06 AMEE1032 pKa = 4.97 DD1033 pKa = 3.05 KK1034 pKa = 9.95 DD1035 pKa = 4.96 TRR1037 pKa = 11.84 EE1038 pKa = 3.87 LWSGEE1043 pKa = 3.84 EE1044 pKa = 4.18 KK1045 pKa = 10.4 TNLMQTQDD1053 pKa = 3.35 PTIQLFPHH1061 pKa = 5.54 QQAKK1065 pKa = 10.89 DD1066 pKa = 3.24 EE1067 pKa = 4.47 ALFKK1071 pKa = 10.13 ITINEE1076 pKa = 4.65 RR1077 pKa = 11.84 IRR1079 pKa = 11.84 LAAPEE1084 pKa = 4.22 TNRR1087 pKa = 11.84 KK1088 pKa = 8.48 ALKK1091 pKa = 9.27 DD1092 pKa = 3.69 TINAGDD1098 pKa = 4.5 LLFEE1102 pKa = 5.19 AYY1104 pKa = 10.18 ASFMNVPRR1112 pKa = 11.84 EE1113 pKa = 3.92 TQPFDD1118 pKa = 3.22 KK1119 pKa = 10.56 RR1120 pKa = 11.84 LWAHH1124 pKa = 6.37 CRR1126 pKa = 11.84 QLALRR1131 pKa = 11.84 TYY1133 pKa = 10.57 LSKK1136 pKa = 9.68 PTSNLQQGAQRR1147 pKa = 11.84 QDD1149 pKa = 2.93 PDD1151 pKa = 3.87 FPDD1154 pKa = 3.11 NAIALFNKK1162 pKa = 8.34 SQWVKK1167 pKa = 10.35 KK1168 pKa = 10.25 LEE1170 pKa = 4.06 KK1171 pKa = 10.47 VGCKK1175 pKa = 9.95 FKK1177 pKa = 11.02 AGQTISAFKK1186 pKa = 10.55 QSTVLLTTTMALYY1199 pKa = 10.13 LRR1201 pKa = 11.84 KK1202 pKa = 9.51 KK1203 pKa = 10.63 RR1204 pKa = 11.84 EE1205 pKa = 3.53 GHH1207 pKa = 5.43 QPDD1210 pKa = 3.93 NVFVMCEE1217 pKa = 3.73 KK1218 pKa = 10.34 TPEE1221 pKa = 4.02 QFNSFVLTKK1230 pKa = 9.95 WDD1232 pKa = 4.4 FSRR1235 pKa = 11.84 PNYY1238 pKa = 9.07 TSDD1241 pKa = 3.01 YY1242 pKa = 8.03 TQYY1245 pKa = 11.34 DD1246 pKa = 3.52 QSQDD1250 pKa = 3.34 SAFLNFEE1257 pKa = 4.11 LRR1259 pKa = 11.84 KK1260 pKa = 9.72 ARR1262 pKa = 11.84 HH1263 pKa = 5.95 FGLPDD1268 pKa = 3.43 EE1269 pKa = 5.15 VVDD1272 pKa = 5.71 FYY1274 pKa = 12.08 AFVKK1278 pKa = 8.76 THH1280 pKa = 6.55 AKK1282 pKa = 9.24 TFLGNLAVMRR1292 pKa = 11.84 LSGEE1296 pKa = 4.14 GPTFDD1301 pKa = 6.1 ANTEE1305 pKa = 4.05 CNIAYY1310 pKa = 8.34 DD1311 pKa = 3.78 ALRR1314 pKa = 11.84 FQLDD1318 pKa = 3.62 PSVNACYY1325 pKa = 10.33 AGDD1328 pKa = 3.77 DD1329 pKa = 3.85 LVRR1332 pKa = 11.84 DD1333 pKa = 4.11 KK1334 pKa = 11.59 ACDD1337 pKa = 3.47 EE1338 pKa = 4.26 RR1339 pKa = 11.84 PGWKK1343 pKa = 9.91 YY1344 pKa = 11.05 SEE1346 pKa = 4.37 PLFSLKK1352 pKa = 10.54 AKK1354 pKa = 10.13 PLVTNKK1360 pKa = 9.9 PDD1362 pKa = 3.19 FCGWRR1367 pKa = 11.84 LTKK1370 pKa = 10.71 YY1371 pKa = 10.53 GIVKK1375 pKa = 10.45 SPIQLYY1381 pKa = 10.26 QSLQLALRR1389 pKa = 11.84 LGKK1392 pKa = 10.19 VEE1394 pKa = 4.03 EE1395 pKa = 4.42 VKK1397 pKa = 10.49 RR1398 pKa = 11.84 SYY1400 pKa = 11.23 AIDD1403 pKa = 3.38 YY1404 pKa = 9.93 LFAYY1408 pKa = 10.43 RR1409 pKa = 11.84 LGDD1412 pKa = 3.38 QLYY1415 pKa = 10.56 DD1416 pKa = 3.39 IFDD1419 pKa = 3.81 EE1420 pKa = 5.39 DD1421 pKa = 4.24 EE1422 pKa = 4.2 LAKK1425 pKa = 10.5 HH1426 pKa = 5.31 QLVTRR1431 pKa = 11.84 TLIKK1435 pKa = 10.51 KK1436 pKa = 9.59 GMQPPSSGEE1445 pKa = 3.8 HH1446 pKa = 6.19 LPTFHH1451 pKa = 6.1 VTSDD1455 pKa = 3.91 RR1456 pKa = 11.84 LIQDD1460 pKa = 3.2 PRR1462 pKa = 11.84 ATKK1465 pKa = 10.55 VSTFEE1470 pKa = 3.82 SDD1472 pKa = 4.03 RR1473 pKa = 11.84 VILPLEE1479 pKa = 4.65 IIDD1482 pKa = 4.01 DD1483 pKa = 4.05 HH1484 pKa = 6.68 FAFASKK1490 pKa = 10.56 SDD1492 pKa = 3.86 RR1493 pKa = 11.84 NDD1495 pKa = 2.59 MTTASNQQTSEE1506 pKa = 4.56 SNVSANQTDD1515 pKa = 3.54 YY1516 pKa = 11.81 GGTLLTDD1523 pKa = 3.97 MFPDD1527 pKa = 3.27 IAMLL1531 pKa = 3.92
Molecular weight: 172.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.401
IPC2_protein 5.347
IPC_protein 5.385
Toseland 5.601
ProMoST 5.639
Dawson 5.512
Bjellqvist 5.588
Wikipedia 5.474
Rodwell 5.461
Grimsley 5.664
Solomon 5.512
Lehninger 5.499
Nozaki 5.715
DTASelect 5.906
Thurlkill 5.753
EMBOSS 5.728
Sillero 5.804
Patrickios 4.151
IPC_peptide 5.525
IPC2_peptide 5.804
IPC2.peptide.svr19 5.76
Protein with the highest isoelectric point:
>tr|A0A0A0YR04|A0A0A0YR04_9VIRU 40kDa protein OS=Garlic virus B OX=12432 GN=ORFIV PE=4 SV=1
MM1 pKa = 7.54 SFTPPPDD8 pKa = 3.31 NSKK11 pKa = 11.04 SILAIAVGAAAALIVFTIRR30 pKa = 11.84 TNHH33 pKa = 5.91 SASVGDD39 pKa = 3.63 NTHH42 pKa = 6.91 RR43 pKa = 11.84 LPYY46 pKa = 9.75 GGQYY50 pKa = 10.1 RR51 pKa = 11.84 DD52 pKa = 3.33 GNKK55 pKa = 9.61 LVNYY59 pKa = 8.82 NGPHH63 pKa = 6.3 TGASPPSILWPALSAIILTLVIHH86 pKa = 6.29 FRR88 pKa = 11.84 SGTRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 ICIHH98 pKa = 6.25 CSEE101 pKa = 4.7 STSAHH106 pKa = 4.7 QSS108 pKa = 3.06
Molecular weight: 11.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.21
IPC2_protein 9.385
IPC_protein 9.911
Toseland 9.736
ProMoST 9.677
Dawson 10.058
Bjellqvist 9.882
Wikipedia 10.306
Rodwell 10.16
Grimsley 10.175
Solomon 10.16
Lehninger 10.116
Nozaki 9.911
DTASelect 9.838
Thurlkill 9.882
EMBOSS 10.189
Sillero 10.014
Patrickios 7.556
IPC_peptide 10.145
IPC2_peptide 9.165
IPC2.peptide.svr19 8.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2610
108
1531
435.0
48.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.548 ± 0.553
1.686 ± 0.274
6.513 ± 0.884
5.556 ± 0.492
4.023 ± 0.512
4.828 ± 0.553
3.18 ± 0.602
5.134 ± 0.623
5.556 ± 0.49
10.345 ± 0.898
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.648 ± 0.352
5.019 ± 0.533
5.862 ± 0.419
4.866 ± 0.456
5.287 ± 0.24
7.05 ± 0.574
7.625 ± 0.602
4.674 ± 0.422
0.69 ± 0.156
2.912 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here