Escherichia phage vB_EcoS-Ro145clw

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S6ZQN6|A0A3S6ZQN6_9CAUD Tape measure protein OS=Escherichia phage vB_EcoS-Ro145clw OX=2079541 GN=vBEcoSRo145clw_00017 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 4.91EE3 pKa = 4.47LICIEE8 pKa = 4.77SEE10 pKa = 3.95PDD12 pKa = 3.29VQGWFVKK19 pKa = 9.94GNKK22 pKa = 9.16YY23 pKa = 10.65SSGVYY28 pKa = 9.16YY29 pKa = 10.58DD30 pKa = 3.09GRR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 10.58VLDD37 pKa = 3.79EE38 pKa = 6.67DD39 pKa = 4.16GDD41 pKa = 3.83EE42 pKa = 4.36WDD44 pKa = 5.21LEE46 pKa = 4.33DD47 pKa = 5.26AVGGTLTISFFNAVAASFTTASNHH71 pKa = 6.48DD72 pKa = 3.62ASADD76 pKa = 3.64DD77 pKa = 4.02SEE79 pKa = 5.01GGCRR83 pKa = 11.84EE84 pKa = 3.93

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S6ZRK9|A0A3S6ZRK9_9CAUD Decoration protein OS=Escherichia phage vB_EcoS-Ro145clw OX=2079541 GN=vBEcoSRo145clw_00003 PE=4 SV=1
MM1 pKa = 7.92IYY3 pKa = 10.33QLYY6 pKa = 9.65RR7 pKa = 11.84AIDD10 pKa = 3.42RR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.3NTEE16 pKa = 3.99ALWLLRR22 pKa = 11.84APSGAHH28 pKa = 5.79RR29 pKa = 11.84MEE31 pKa = 3.82EE32 pKa = 3.68MAYY35 pKa = 9.87LGKK38 pKa = 10.38VPRR41 pKa = 11.84PKK43 pKa = 10.64DD44 pKa = 2.83IGRR47 pKa = 11.84RR48 pKa = 11.84VSQIKK53 pKa = 8.62RR54 pKa = 11.84TTFAKK59 pKa = 9.65PDD61 pKa = 3.96FYY63 pKa = 11.43VFEE66 pKa = 4.49SMYY69 pKa = 10.09GWAMHH74 pKa = 6.17CQHH77 pKa = 5.67QTRR80 pKa = 11.84HH81 pKa = 6.64LIDD84 pKa = 3.05QWEE87 pKa = 4.03VRR89 pKa = 11.84NDD91 pKa = 3.57FKK93 pKa = 10.71RR94 pKa = 11.84TRR96 pKa = 11.84RR97 pKa = 11.84QQ98 pKa = 2.93

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12196

36

851

182.0

20.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.724 ± 0.555

1.091 ± 0.158

6.117 ± 0.232

6.83 ± 0.418

3.846 ± 0.154

7.732 ± 0.334

1.566 ± 0.175

5.354 ± 0.201

5.797 ± 0.373

7.666 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.181

4.428 ± 0.304

3.936 ± 0.317

3.682 ± 0.355

5.436 ± 0.265

5.625 ± 0.275

6.502 ± 0.401

7.396 ± 0.344

1.32 ± 0.159

3.395 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski