Amino acid dipepetide frequency for Escherichia phage vB_EcoS-Ro145clw

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.136AlaAla: 12.136 ± 1.568
0.902AlaCys: 0.902 ± 0.228
5.74AlaAsp: 5.74 ± 0.816
7.052AlaGlu: 7.052 ± 0.593
3.772AlaPhe: 3.772 ± 0.557
7.052AlaGly: 7.052 ± 0.933
0.984AlaHis: 0.984 ± 0.304
5.248AlaIle: 5.248 ± 0.833
5.904AlaLys: 5.904 ± 0.844
8.118AlaLeu: 8.118 ± 0.935
1.968AlaMet: 1.968 ± 0.416
4.018AlaAsn: 4.018 ± 0.55
3.608AlaPro: 3.608 ± 0.499
3.362AlaGln: 3.362 ± 0.7
4.182AlaArg: 4.182 ± 0.574
5.74AlaSer: 5.74 ± 0.772
6.478AlaThr: 6.478 ± 0.766
6.56AlaVal: 6.56 ± 0.727
1.312AlaTrp: 1.312 ± 0.258
3.116AlaTyr: 3.116 ± 0.496
0.0AlaXaa: 0.0 ± 0.0
Cys
1.066CysAla: 1.066 ± 0.277
0.246CysCys: 0.246 ± 0.157
1.148CysAsp: 1.148 ± 0.325
0.984CysGlu: 0.984 ± 0.402
0.328CysPhe: 0.328 ± 0.185
1.312CysGly: 1.312 ± 0.454
0.246CysHis: 0.246 ± 0.143
0.82CysIle: 0.82 ± 0.301
0.41CysLys: 0.41 ± 0.174
0.492CysLeu: 0.492 ± 0.246
0.164CysMet: 0.164 ± 0.148
0.41CysAsn: 0.41 ± 0.204
0.246CysPro: 0.246 ± 0.15
0.246CysGln: 0.246 ± 0.128
0.738CysArg: 0.738 ± 0.222
0.492CysSer: 0.492 ± 0.161
0.574CysThr: 0.574 ± 0.28
0.492CysVal: 0.492 ± 0.213
0.246CysTrp: 0.246 ± 0.154
0.246CysTyr: 0.246 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
7.216AspAla: 7.216 ± 0.815
0.41AspCys: 0.41 ± 0.191
4.1AspAsp: 4.1 ± 0.544
5.002AspGlu: 5.002 ± 0.676
2.952AspPhe: 2.952 ± 0.507
5.002AspGly: 5.002 ± 0.688
0.902AspHis: 0.902 ± 0.301
4.592AspIle: 4.592 ± 0.672
2.706AspLys: 2.706 ± 0.409
5.494AspLeu: 5.494 ± 0.692
1.394AspMet: 1.394 ± 0.235
2.05AspAsn: 2.05 ± 0.429
1.804AspPro: 1.804 ± 0.376
0.902AspGln: 0.902 ± 0.306
2.706AspArg: 2.706 ± 0.449
2.87AspSer: 2.87 ± 0.547
3.772AspThr: 3.772 ± 0.462
4.018AspVal: 4.018 ± 0.504
0.82AspTrp: 0.82 ± 0.201
2.46AspTyr: 2.46 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
5.33GluAla: 5.33 ± 0.674
0.492GluCys: 0.492 ± 0.194
4.264GluAsp: 4.264 ± 0.565
5.33GluGlu: 5.33 ± 0.919
2.624GluPhe: 2.624 ± 0.54
4.838GluGly: 4.838 ± 0.794
0.574GluHis: 0.574 ± 0.211
3.854GluIle: 3.854 ± 0.684
3.526GluLys: 3.526 ± 0.685
6.396GluLeu: 6.396 ± 0.682
2.952GluMet: 2.952 ± 0.497
2.87GluAsn: 2.87 ± 0.501
1.968GluPro: 1.968 ± 0.472
3.034GluGln: 3.034 ± 0.809
3.608GluArg: 3.608 ± 0.564
3.772GluSer: 3.772 ± 0.626
3.526GluThr: 3.526 ± 0.524
5.576GluVal: 5.576 ± 0.633
1.23GluTrp: 1.23 ± 0.372
2.542GluTyr: 2.542 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
2.624PheAla: 2.624 ± 0.48
0.492PheCys: 0.492 ± 0.226
3.198PheAsp: 3.198 ± 0.495
2.87PheGlu: 2.87 ± 0.449
1.23PhePhe: 1.23 ± 0.367
3.69PheGly: 3.69 ± 0.55
0.656PheHis: 0.656 ± 0.276
3.116PheIle: 3.116 ± 0.507
2.788PheLys: 2.788 ± 0.516
1.804PheLeu: 1.804 ± 0.405
0.656PheMet: 0.656 ± 0.225
1.968PheAsn: 1.968 ± 0.426
1.148PhePro: 1.148 ± 0.302
1.23PheGln: 1.23 ± 0.306
1.722PheArg: 1.722 ± 0.272
2.214PheSer: 2.214 ± 0.505
3.116PheThr: 3.116 ± 0.424
1.968PheVal: 1.968 ± 0.468
0.738PheTrp: 0.738 ± 0.238
1.23PheTyr: 1.23 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
6.478GlyAla: 6.478 ± 0.818
1.066GlyCys: 1.066 ± 0.273
4.018GlyAsp: 4.018 ± 0.44
5.74GlyGlu: 5.74 ± 0.595
3.69GlyPhe: 3.69 ± 0.566
5.658GlyGly: 5.658 ± 0.74
1.886GlyHis: 1.886 ± 0.421
3.116GlyIle: 3.116 ± 0.7
5.658GlyLys: 5.658 ± 0.838
5.494GlyLeu: 5.494 ± 0.708
2.46GlyMet: 2.46 ± 0.607
3.69GlyAsn: 3.69 ± 0.739
2.214GlyPro: 2.214 ± 0.435
3.034GlyGln: 3.034 ± 0.581
3.608GlyArg: 3.608 ± 0.522
4.264GlySer: 4.264 ± 0.603
4.346GlyThr: 4.346 ± 0.495
6.806GlyVal: 6.806 ± 0.823
1.394GlyTrp: 1.394 ± 0.331
2.706GlyTyr: 2.706 ± 0.511
0.0GlyXaa: 0.0 ± 0.0
His
1.148HisAla: 1.148 ± 0.296
0.246HisCys: 0.246 ± 0.13
0.902HisAsp: 0.902 ± 0.242
1.066HisGlu: 1.066 ± 0.357
0.738HisPhe: 0.738 ± 0.287
0.902HisGly: 0.902 ± 0.326
0.656HisHis: 0.656 ± 0.273
0.82HisIle: 0.82 ± 0.228
1.394HisLys: 1.394 ± 0.382
0.984HisLeu: 0.984 ± 0.342
0.246HisMet: 0.246 ± 0.149
0.656HisAsn: 0.656 ± 0.274
0.984HisPro: 0.984 ± 0.288
0.82HisGln: 0.82 ± 0.223
0.902HisArg: 0.902 ± 0.277
0.738HisSer: 0.738 ± 0.237
0.738HisThr: 0.738 ± 0.294
1.312HisVal: 1.312 ± 0.441
0.082HisTrp: 0.082 ± 0.079
0.328HisTyr: 0.328 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
5.494IleAla: 5.494 ± 0.681
0.984IleCys: 0.984 ± 0.248
4.51IleAsp: 4.51 ± 0.631
3.198IleGlu: 3.198 ± 0.484
1.558IlePhe: 1.558 ± 0.387
3.444IleGly: 3.444 ± 0.529
0.492IleHis: 0.492 ± 0.166
2.46IleIle: 2.46 ± 0.368
2.788IleLys: 2.788 ± 0.503
2.952IleLeu: 2.952 ± 0.592
0.656IleMet: 0.656 ± 0.286
2.952IleAsn: 2.952 ± 0.418
3.034IlePro: 3.034 ± 0.417
1.722IleGln: 1.722 ± 0.432
2.706IleArg: 2.706 ± 0.412
3.772IleSer: 3.772 ± 0.537
4.346IleThr: 4.346 ± 0.55
3.854IleVal: 3.854 ± 0.56
0.984IleTrp: 0.984 ± 0.243
1.64IleTyr: 1.64 ± 0.393
0.0IleXaa: 0.0 ± 0.0
Lys
5.494LysAla: 5.494 ± 0.717
0.984LysCys: 0.984 ± 0.352
3.526LysAsp: 3.526 ± 0.619
3.854LysGlu: 3.854 ± 0.728
1.968LysPhe: 1.968 ± 0.332
3.69LysGly: 3.69 ± 0.584
0.82LysHis: 0.82 ± 0.232
1.968LysIle: 1.968 ± 0.429
3.198LysLys: 3.198 ± 0.534
5.412LysLeu: 5.412 ± 0.67
2.542LysMet: 2.542 ± 0.537
1.886LysAsn: 1.886 ± 0.376
1.722LysPro: 1.722 ± 0.355
2.706LysGln: 2.706 ± 0.517
3.936LysArg: 3.936 ± 0.576
3.034LysSer: 3.034 ± 0.641
4.264LysThr: 4.264 ± 0.696
3.198LysVal: 3.198 ± 0.646
0.82LysTrp: 0.82 ± 0.243
2.952LysTyr: 2.952 ± 0.527
0.0LysXaa: 0.0 ± 0.0
Leu
7.872LeuAla: 7.872 ± 0.789
0.82LeuCys: 0.82 ± 0.279
4.92LeuAsp: 4.92 ± 0.664
5.412LeuGlu: 5.412 ± 0.933
2.788LeuPhe: 2.788 ± 0.668
5.494LeuGly: 5.494 ± 0.601
0.902LeuHis: 0.902 ± 0.25
4.51LeuIle: 4.51 ± 0.412
4.182LeuLys: 4.182 ± 0.724
5.658LeuLeu: 5.658 ± 0.791
1.886LeuMet: 1.886 ± 0.381
3.772LeuAsn: 3.772 ± 0.666
3.362LeuPro: 3.362 ± 0.543
2.706LeuGln: 2.706 ± 0.43
5.33LeuArg: 5.33 ± 0.709
3.526LeuSer: 3.526 ± 0.447
5.33LeuThr: 5.33 ± 0.64
4.92LeuVal: 4.92 ± 0.571
1.23LeuTrp: 1.23 ± 0.366
2.05LeuTyr: 2.05 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
2.706MetAla: 2.706 ± 0.422
0.41MetCys: 0.41 ± 0.172
0.738MetAsp: 0.738 ± 0.263
1.148MetGlu: 1.148 ± 0.349
0.902MetPhe: 0.902 ± 0.278
1.886MetGly: 1.886 ± 0.474
0.328MetHis: 0.328 ± 0.159
1.394MetIle: 1.394 ± 0.345
1.968MetLys: 1.968 ± 0.508
2.05MetLeu: 2.05 ± 0.449
0.738MetMet: 0.738 ± 0.231
0.656MetAsn: 0.656 ± 0.243
1.558MetPro: 1.558 ± 0.414
0.41MetGln: 0.41 ± 0.207
0.984MetArg: 0.984 ± 0.259
2.05MetSer: 2.05 ± 0.341
2.542MetThr: 2.542 ± 0.552
2.132MetVal: 2.132 ± 0.474
0.328MetTrp: 0.328 ± 0.165
0.656MetTyr: 0.656 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
3.936AsnAla: 3.936 ± 0.474
0.738AsnCys: 0.738 ± 0.27
2.132AsnAsp: 2.132 ± 0.387
2.378AsnGlu: 2.378 ± 0.38
1.312AsnPhe: 1.312 ± 0.305
4.428AsnGly: 4.428 ± 0.626
0.82AsnHis: 0.82 ± 0.313
2.542AsnIle: 2.542 ± 0.552
2.132AsnLys: 2.132 ± 0.522
2.952AsnLeu: 2.952 ± 0.567
0.82AsnMet: 0.82 ± 0.272
2.624AsnAsn: 2.624 ± 0.727
1.804AsnPro: 1.804 ± 0.359
1.804AsnGln: 1.804 ± 0.435
1.968AsnArg: 1.968 ± 0.414
2.624AsnSer: 2.624 ± 0.501
3.444AsnThr: 3.444 ± 0.809
3.69AsnVal: 3.69 ± 0.646
0.41AsnTrp: 0.41 ± 0.152
1.722AsnTyr: 1.722 ± 0.372
0.0AsnXaa: 0.0 ± 0.0
Pro
2.87ProAla: 2.87 ± 0.389
0.246ProCys: 0.246 ± 0.15
3.526ProAsp: 3.526 ± 0.434
4.018ProGlu: 4.018 ± 0.692
1.722ProPhe: 1.722 ± 0.369
3.28ProGly: 3.28 ± 0.587
0.41ProHis: 0.41 ± 0.142
1.394ProIle: 1.394 ± 0.298
1.64ProLys: 1.64 ± 0.38
3.362ProLeu: 3.362 ± 0.512
0.738ProMet: 0.738 ± 0.238
1.394ProAsn: 1.394 ± 0.309
1.558ProPro: 1.558 ± 0.388
1.148ProGln: 1.148 ± 0.302
1.804ProArg: 1.804 ± 0.443
2.706ProSer: 2.706 ± 0.415
2.214ProThr: 2.214 ± 0.494
4.182ProVal: 4.182 ± 0.631
0.164ProTrp: 0.164 ± 0.105
0.984ProTyr: 0.984 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
3.69GlnAla: 3.69 ± 0.703
0.328GlnCys: 0.328 ± 0.14
2.05GlnAsp: 2.05 ± 0.396
1.968GlnGlu: 1.968 ± 0.442
1.23GlnPhe: 1.23 ± 0.275
1.64GlnGly: 1.64 ± 0.288
0.82GlnHis: 0.82 ± 0.259
2.214GlnIle: 2.214 ± 0.425
2.378GlnLys: 2.378 ± 0.45
3.116GlnLeu: 3.116 ± 0.452
1.312GlnMet: 1.312 ± 0.345
1.558GlnAsn: 1.558 ± 0.428
1.722GlnPro: 1.722 ± 0.322
2.132GlnGln: 2.132 ± 0.716
2.542GlnArg: 2.542 ± 0.484
1.804GlnSer: 1.804 ± 0.523
1.968GlnThr: 1.968 ± 0.471
2.46GlnVal: 2.46 ± 0.476
0.82GlnTrp: 0.82 ± 0.227
1.066GlnTyr: 1.066 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
4.264ArgAla: 4.264 ± 0.476
0.41ArgCys: 0.41 ± 0.185
2.952ArgAsp: 2.952 ± 0.425
3.362ArgGlu: 3.362 ± 0.623
1.722ArgPhe: 1.722 ± 0.367
4.264ArgGly: 4.264 ± 0.588
1.23ArgHis: 1.23 ± 0.253
2.706ArgIle: 2.706 ± 0.573
4.264ArgLys: 4.264 ± 0.606
3.772ArgLeu: 3.772 ± 0.474
1.394ArgMet: 1.394 ± 0.367
3.362ArgAsn: 3.362 ± 0.447
2.05ArgPro: 2.05 ± 0.407
2.296ArgGln: 2.296 ± 0.355
4.674ArgArg: 4.674 ± 0.764
3.034ArgSer: 3.034 ± 0.386
2.378ArgThr: 2.378 ± 0.496
4.428ArgVal: 4.428 ± 0.673
0.41ArgTrp: 0.41 ± 0.162
1.394ArgTyr: 1.394 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
5.494SerAla: 5.494 ± 0.81
0.328SerCys: 0.328 ± 0.139
2.952SerAsp: 2.952 ± 0.427
2.87SerGlu: 2.87 ± 0.498
2.05SerPhe: 2.05 ± 0.475
6.396SerGly: 6.396 ± 0.621
0.656SerHis: 0.656 ± 0.216
2.952SerIle: 2.952 ± 0.687
3.526SerLys: 3.526 ± 0.623
4.264SerLeu: 4.264 ± 0.617
1.394SerMet: 1.394 ± 0.364
2.788SerAsn: 2.788 ± 0.458
2.624SerPro: 2.624 ± 0.457
1.804SerGln: 1.804 ± 0.394
2.706SerArg: 2.706 ± 0.493
2.87SerSer: 2.87 ± 0.627
3.69SerThr: 3.69 ± 0.589
4.018SerVal: 4.018 ± 0.437
0.492SerTrp: 0.492 ± 0.176
2.378SerTyr: 2.378 ± 0.389
0.0SerXaa: 0.0 ± 0.0
Thr
7.626ThrAla: 7.626 ± 0.838
0.328ThrCys: 0.328 ± 0.196
3.772ThrAsp: 3.772 ± 0.462
3.526ThrGlu: 3.526 ± 0.487
2.87ThrPhe: 2.87 ± 0.54
6.232ThrGly: 6.232 ± 0.909
1.066ThrHis: 1.066 ± 0.32
3.526ThrIle: 3.526 ± 0.486
3.362ThrLys: 3.362 ± 0.445
5.002ThrLeu: 5.002 ± 0.693
1.64ThrMet: 1.64 ± 0.389
1.886ThrAsn: 1.886 ± 0.44
4.428ThrPro: 4.428 ± 0.688
2.05ThrGln: 2.05 ± 0.355
2.952ThrArg: 2.952 ± 0.376
3.034ThrSer: 3.034 ± 0.439
3.526ThrThr: 3.526 ± 0.628
5.166ThrVal: 5.166 ± 0.61
0.738ThrTrp: 0.738 ± 0.239
2.214ThrTyr: 2.214 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
7.216ValAla: 7.216 ± 0.665
0.656ValCys: 0.656 ± 0.228
3.608ValAsp: 3.608 ± 0.382
5.822ValGlu: 5.822 ± 0.734
2.214ValPhe: 2.214 ± 0.323
4.592ValGly: 4.592 ± 0.662
1.23ValHis: 1.23 ± 0.288
4.428ValIle: 4.428 ± 0.653
3.608ValLys: 3.608 ± 0.494
5.494ValLeu: 5.494 ± 0.685
1.394ValMet: 1.394 ± 0.341
3.444ValAsn: 3.444 ± 0.529
2.214ValPro: 2.214 ± 0.548
3.116ValGln: 3.116 ± 0.457
4.1ValArg: 4.1 ± 0.551
4.92ValSer: 4.92 ± 0.728
6.724ValThr: 6.724 ± 0.911
5.74ValVal: 5.74 ± 0.704
0.492ValTrp: 0.492 ± 0.222
2.952ValTyr: 2.952 ± 0.535
0.0ValXaa: 0.0 ± 0.0
Trp
1.312TrpAla: 1.312 ± 0.346
0.164TrpCys: 0.164 ± 0.108
0.738TrpAsp: 0.738 ± 0.223
0.328TrpGlu: 0.328 ± 0.153
1.312TrpPhe: 1.312 ± 0.327
0.902TrpGly: 0.902 ± 0.298
0.41TrpHis: 0.41 ± 0.238
0.164TrpIle: 0.164 ± 0.114
0.738TrpLys: 0.738 ± 0.213
1.476TrpLeu: 1.476 ± 0.31
0.41TrpMet: 0.41 ± 0.16
0.656TrpAsn: 0.656 ± 0.301
0.328TrpPro: 0.328 ± 0.199
0.574TrpGln: 0.574 ± 0.258
0.738TrpArg: 0.738 ± 0.297
0.656TrpSer: 0.656 ± 0.244
0.328TrpThr: 0.328 ± 0.17
1.312TrpVal: 1.312 ± 0.292
0.164TrpTrp: 0.164 ± 0.104
0.492TrpTyr: 0.492 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.28TyrAla: 3.28 ± 0.473
0.656TyrCys: 0.656 ± 0.226
2.378TyrAsp: 2.378 ± 0.481
2.378TyrGlu: 2.378 ± 0.408
1.476TyrPhe: 1.476 ± 0.397
2.624TyrGly: 2.624 ± 0.354
0.574TyrHis: 0.574 ± 0.234
1.722TyrIle: 1.722 ± 0.428
1.804TyrLys: 1.804 ± 0.421
2.378TyrLeu: 2.378 ± 0.384
0.738TyrMet: 0.738 ± 0.215
1.64TyrAsn: 1.64 ± 0.4
0.984TyrPro: 0.984 ± 0.459
1.722TyrGln: 1.722 ± 0.337
2.46TyrArg: 2.46 ± 0.388
2.132TyrSer: 2.132 ± 0.402
1.722TyrThr: 1.722 ± 0.341
2.132TyrVal: 2.132 ± 0.42
0.328TyrTrp: 0.328 ± 0.181
0.82TyrTyr: 0.82 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12196 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski