Amino acid dipepetide frequency for Pedobacter sp. AR-3-17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.239AlaAla: 5.239 ± 0.109	0.609AlaCys: 0.609 ± 0.024	3.99AlaAsp: 3.99 ± 0.06	3.993AlaGlu: 3.993 ± 0.063	3.466AlaPhe: 3.466 ± 0.052	4.896AlaGly: 4.896 ± 0.096	1.097AlaHis: 1.097 ± 0.035	5.657AlaIle: 5.657 ± 0.073	5.275AlaLys: 5.275 ± 0.096	6.568AlaLeu: 6.568 ± 0.09	1.496AlaMet: 1.496 ± 0.04	4.179AlaAsn: 4.179 ± 0.1	2.041AlaPro: 2.041 ± 0.046	2.891AlaGln: 2.891 ± 0.052	1.924AlaArg: 1.924 ± 0.047	4.336AlaSer: 4.336 ± 0.072	4.019AlaThr: 4.019 ± 0.096	4.49AlaVal: 4.49 ± 0.069	0.68AlaTrp: 0.68 ± 0.028	2.712AlaTyr: 2.712 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.509CysAla: 0.509 ± 0.02	0.102CysCys: 0.102 ± 0.011	0.398CysAsp: 0.398 ± 0.018	0.448CysGlu: 0.448 ± 0.022	0.464CysPhe: 0.464 ± 0.023	0.62CysGly: 0.62 ± 0.026	0.173CysHis: 0.173 ± 0.014	0.598CysIle: 0.598 ± 0.022	0.504CysLys: 0.504 ± 0.023	0.735CysLeu: 0.735 ± 0.025	0.147CysMet: 0.147 ± 0.011	0.409CysAsn: 0.409 ± 0.02	0.33CysPro: 0.33 ± 0.019	0.199CysGln: 0.199 ± 0.011	0.234CysArg: 0.234 ± 0.014	0.51CysSer: 0.51 ± 0.019	0.4CysThr: 0.4 ± 0.022	0.428CysVal: 0.428 ± 0.021	0.075CysTrp: 0.075 ± 0.008	0.284CysTyr: 0.284 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.958AspAla: 3.958 ± 0.075	0.394AspCys: 0.394 ± 0.018	2.719AspAsp: 2.719 ± 0.058	3.475AspGlu: 3.475 ± 0.056	3.521AspPhe: 3.521 ± 0.065	3.717AspGly: 3.717 ± 0.076	0.932AspHis: 0.932 ± 0.026	4.049AspIle: 4.049 ± 0.069	4.048AspLys: 4.048 ± 0.06	5.505AspLeu: 5.505 ± 0.071	1.0AspMet: 1.0 ± 0.033	2.52AspAsn: 2.52 ± 0.052	1.776AspPro: 1.776 ± 0.037	1.99AspGln: 1.99 ± 0.042	1.812AspArg: 1.812 ± 0.04	2.663AspSer: 2.663 ± 0.047	2.27AspThr: 2.27 ± 0.044	3.294AspVal: 3.294 ± 0.058	0.694AspTrp: 0.694 ± 0.026	2.412AspTyr: 2.412 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	4.031GluAla: 4.031 ± 0.068	0.314GluCys: 0.314 ± 0.019	2.936GluAsp: 2.936 ± 0.061	4.313GluGlu: 4.313 ± 0.089	2.748GluPhe: 2.748 ± 0.047	3.364GluGly: 3.364 ± 0.056	0.926GluHis: 0.926 ± 0.029	5.378GluIle: 5.378 ± 0.076	5.43GluLys: 5.43 ± 0.089	5.706GluLeu: 5.706 ± 0.079	1.481GluMet: 1.481 ± 0.039	3.989GluAsn: 3.989 ± 0.063	1.525GluPro: 1.525 ± 0.048	2.242GluGln: 2.242 ± 0.055	2.204GluArg: 2.204 ± 0.049	3.041GluSer: 3.041 ± 0.054	3.094GluThr: 3.094 ± 0.054	4.014GluVal: 4.014 ± 0.055	0.61GluTrp: 0.61 ± 0.023	2.011GluTyr: 2.011 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.418PheAla: 3.418 ± 0.064	0.503PheCys: 0.503 ± 0.02	3.096PheAsp: 3.096 ± 0.054	3.13PheGlu: 3.13 ± 0.058	2.718PhePhe: 2.718 ± 0.053	3.632PheGly: 3.632 ± 0.063	0.845PheHis: 0.845 ± 0.027	4.075PheIle: 4.075 ± 0.065	4.097PheLys: 4.097 ± 0.07	4.797PheLeu: 4.797 ± 0.077	1.053PheMet: 1.053 ± 0.027	3.547PheAsn: 3.547 ± 0.054	1.714PhePro: 1.714 ± 0.035	1.462PheGln: 1.462 ± 0.04	1.603PheArg: 1.603 ± 0.039	4.115PheSer: 4.115 ± 0.066	3.203PheThr: 3.203 ± 0.052	2.898PheVal: 2.898 ± 0.062	0.597PheTrp: 0.597 ± 0.024	2.218PheTyr: 2.218 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.498GlyAla: 4.498 ± 0.109	0.628GlyCys: 0.628 ± 0.026	3.262GlyAsp: 3.262 ± 0.075	3.452GlyGlu: 3.452 ± 0.054	3.898GlyPhe: 3.898 ± 0.063	4.728GlyGly: 4.728 ± 0.102	1.031GlyHis: 1.031 ± 0.035	5.444GlyIle: 5.444 ± 0.084	5.359GlyLys: 5.359 ± 0.076	6.296GlyLeu: 6.296 ± 0.096	1.527GlyMet: 1.527 ± 0.046	3.809GlyAsn: 3.809 ± 0.077	1.406GlyPro: 1.406 ± 0.038	1.825GlyGln: 1.825 ± 0.039	2.155GlyArg: 2.155 ± 0.05	4.283GlySer: 4.283 ± 0.088	3.956GlyThr: 3.956 ± 0.091	4.329GlyVal: 4.329 ± 0.067	0.805GlyTrp: 0.805 ± 0.028	2.771GlyTyr: 2.771 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.932HisAla: 0.932 ± 0.029	0.183HisCys: 0.183 ± 0.012	0.72HisAsp: 0.72 ± 0.022	0.911HisGlu: 0.911 ± 0.029	1.072HisPhe: 1.072 ± 0.031	0.957HisGly: 0.957 ± 0.033	0.574HisHis: 0.574 ± 0.025	1.333HisIle: 1.333 ± 0.035	1.03HisLys: 1.03 ± 0.028	1.824HisLeu: 1.824 ± 0.052	0.275HisMet: 0.275 ± 0.017	0.769HisAsn: 0.769 ± 0.024	0.978HisPro: 0.978 ± 0.032	1.094HisGln: 1.094 ± 0.033	0.586HisArg: 0.586 ± 0.021	0.991HisSer: 0.991 ± 0.032	0.834HisThr: 0.834 ± 0.029	0.785HisVal: 0.785 ± 0.026	0.217HisTrp: 0.217 ± 0.014	0.762HisTyr: 0.762 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.926IleAla: 5.926 ± 0.078	0.711IleCys: 0.711 ± 0.024	4.473IleAsp: 4.473 ± 0.058	4.721IleGlu: 4.721 ± 0.071	3.675IlePhe: 3.675 ± 0.076	5.018IleGly: 5.018 ± 0.077	1.318IleHis: 1.318 ± 0.034	6.499IleIle: 6.499 ± 0.108	6.542IleLys: 6.542 ± 0.094	7.243IleLeu: 7.243 ± 0.095	1.377IleMet: 1.377 ± 0.033	5.222IleAsn: 5.222 ± 0.075	3.276IlePro: 3.276 ± 0.061	2.575IleGln: 2.575 ± 0.043	2.523IleArg: 2.523 ± 0.051	5.84IleSer: 5.84 ± 0.081	4.795IleThr: 4.795 ± 0.085	4.354IleVal: 4.354 ± 0.067	0.762IleTrp: 0.762 ± 0.026	3.051IleTyr: 3.051 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.465LysAla: 5.465 ± 0.078	0.361LysCys: 0.361 ± 0.019	4.473LysAsp: 4.473 ± 0.077	5.695LysGlu: 5.695 ± 0.086	3.174LysPhe: 3.174 ± 0.053	4.891LysGly: 4.891 ± 0.086	1.232LysHis: 1.232 ± 0.034	6.515LysIle: 6.515 ± 0.081	6.491LysLys: 6.491 ± 0.101	6.926LysLeu: 6.926 ± 0.093	2.054LysMet: 2.054 ± 0.046	5.43LysAsn: 5.43 ± 0.087	2.763LysPro: 2.763 ± 0.055	2.843LysGln: 2.843 ± 0.054	2.675LysArg: 2.675 ± 0.052	4.74LysSer: 4.74 ± 0.075	4.75LysThr: 4.75 ± 0.072	5.003LysVal: 5.003 ± 0.082	0.81LysTrp: 0.81 ± 0.028	3.069LysTyr: 3.069 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	6.593LeuAla: 6.593 ± 0.083	0.696LeuCys: 0.696 ± 0.029	4.881LeuAsp: 4.881 ± 0.067	5.303LeuGlu: 5.303 ± 0.072	4.903LeuPhe: 4.903 ± 0.079	5.762LeuGly: 5.762 ± 0.088	1.516LeuHis: 1.516 ± 0.041	7.557LeuIle: 7.557 ± 0.118	8.61LeuLys: 8.61 ± 0.109	8.939LeuLeu: 8.939 ± 0.127	2.141LeuMet: 2.141 ± 0.043	6.542LeuAsn: 6.542 ± 0.094	3.609LeuPro: 3.609 ± 0.057	3.28LeuGln: 3.28 ± 0.051	3.179LeuArg: 3.179 ± 0.057	7.03LeuSer: 7.03 ± 0.088	5.412LeuThr: 5.412 ± 0.081	5.436LeuVal: 5.436 ± 0.068	0.877LeuTrp: 0.877 ± 0.033	3.006LeuTyr: 3.006 ± 0.055	0.001LeuXaa: 0.001 ± 0.001
Met	1.659MetAla: 1.659 ± 0.042	0.119MetCys: 0.119 ± 0.01	1.11MetAsp: 1.11 ± 0.033	1.184MetGlu: 1.184 ± 0.036	0.779MetPhe: 0.779 ± 0.028	1.446MetGly: 1.446 ± 0.039	0.337MetHis: 0.337 ± 0.017	1.658MetIle: 1.658 ± 0.047	2.068MetLys: 2.068 ± 0.044	1.889MetLeu: 1.889 ± 0.043	0.666MetMet: 0.666 ± 0.026	1.261MetAsn: 1.261 ± 0.034	0.906MetPro: 0.906 ± 0.032	0.826MetGln: 0.826 ± 0.026	0.752MetArg: 0.752 ± 0.026	1.288MetSer: 1.288 ± 0.036	0.949MetThr: 0.949 ± 0.03	1.396MetVal: 1.396 ± 0.036	0.178MetTrp: 0.178 ± 0.015	0.568MetTyr: 0.568 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	4.123AsnAla: 4.123 ± 0.073	0.475AsnCys: 0.475 ± 0.025	2.891AsnAsp: 2.891 ± 0.05	3.339AsnGlu: 3.339 ± 0.056	3.319AsnPhe: 3.319 ± 0.062	4.357AsnGly: 4.357 ± 0.092	1.131AsnHis: 1.131 ± 0.028	4.871AsnIle: 4.871 ± 0.069	4.49AsnLys: 4.49 ± 0.075	5.945AsnLeu: 5.945 ± 0.072	1.202AsnMet: 1.202 ± 0.036	3.994AsnAsn: 3.994 ± 0.075	2.896AsnPro: 2.896 ± 0.054	2.83AsnGln: 2.83 ± 0.056	2.1AsnArg: 2.1 ± 0.048	3.919AsnSer: 3.919 ± 0.069	3.456AsnThr: 3.456 ± 0.086	3.312AsnVal: 3.312 ± 0.06	0.841AsnTrp: 0.841 ± 0.031	3.023AsnTyr: 3.023 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.437ProAla: 2.437 ± 0.051	0.204ProCys: 0.204 ± 0.015	2.119ProAsp: 2.119 ± 0.043	2.437ProGlu: 2.437 ± 0.041	1.995ProPhe: 1.995 ± 0.046	2.004ProGly: 2.004 ± 0.048	0.637ProHis: 0.637 ± 0.024	2.702ProIle: 2.702 ± 0.055	2.521ProLys: 2.521 ± 0.051	3.155ProLeu: 3.155 ± 0.062	0.665ProMet: 0.665 ± 0.025	2.264ProAsn: 2.264 ± 0.049	0.789ProPro: 0.789 ± 0.028	1.407ProGln: 1.407 ± 0.037	0.92ProArg: 0.92 ± 0.033	2.263ProSer: 2.263 ± 0.048	2.044ProThr: 2.044 ± 0.056	2.575ProVal: 2.575 ± 0.053	0.353ProTrp: 0.353 ± 0.017	1.387ProTyr: 1.387 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.316GlnAla: 2.316 ± 0.048	0.165GlnCys: 0.165 ± 0.012	1.704GlnAsp: 1.704 ± 0.036	2.264GlnGlu: 2.264 ± 0.054	1.82GlnPhe: 1.82 ± 0.038	1.806GlnGly: 1.806 ± 0.045	0.705GlnHis: 0.705 ± 0.026	3.016GlnIle: 3.016 ± 0.045	3.234GlnLys: 3.234 ± 0.062	3.781GlnLeu: 3.781 ± 0.065	0.818GlnMet: 0.818 ± 0.031	2.535GlnAsn: 2.535 ± 0.051	1.276GlnPro: 1.276 ± 0.034	1.852GlnGln: 1.852 ± 0.047	1.22GlnArg: 1.22 ± 0.036	2.183GlnSer: 2.183 ± 0.047	2.238GlnThr: 2.238 ± 0.047	2.071GlnVal: 2.071 ± 0.042	0.375GlnTrp: 0.375 ± 0.019	1.357GlnTyr: 1.357 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	1.965ArgAla: 1.965 ± 0.044	0.194ArgCys: 0.194 ± 0.012	1.66ArgAsp: 1.66 ± 0.038	1.987ArgGlu: 1.987 ± 0.045	1.86ArgPhe: 1.86 ± 0.039	1.977ArgGly: 1.977 ± 0.046	0.471ArgHis: 0.471 ± 0.02	2.736ArgIle: 2.736 ± 0.049	2.602ArgLys: 2.602 ± 0.047	3.163ArgLeu: 3.163 ± 0.061	0.825ArgMet: 0.825 ± 0.031	2.039ArgAsn: 2.039 ± 0.046	1.058ArgPro: 1.058 ± 0.034	1.024ArgGln: 1.024 ± 0.036	1.246ArgArg: 1.246 ± 0.04	1.947ArgSer: 1.947 ± 0.045	1.819ArgThr: 1.819 ± 0.044	2.146ArgVal: 2.146 ± 0.042	0.415ArgTrp: 0.415 ± 0.02	1.477ArgTyr: 1.477 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.488SerAla: 4.488 ± 0.078	0.58SerCys: 0.58 ± 0.022	3.206SerAsp: 3.206 ± 0.057	3.365SerGlu: 3.365 ± 0.051	4.048SerPhe: 4.048 ± 0.062	4.668SerGly: 4.668 ± 0.093	0.976SerHis: 0.976 ± 0.029	5.241SerIle: 5.241 ± 0.079	4.838SerLys: 4.838 ± 0.063	6.591SerLeu: 6.591 ± 0.083	1.189SerMet: 1.189 ± 0.028	3.808SerAsn: 3.808 ± 0.07	2.257SerPro: 2.257 ± 0.049	2.126SerGln: 2.126 ± 0.051	2.091SerArg: 2.091 ± 0.047	4.571SerSer: 4.571 ± 0.092	3.694SerThr: 3.694 ± 0.082	4.01SerVal: 4.01 ± 0.068	0.725SerTrp: 0.725 ± 0.028	2.786SerTyr: 2.786 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.343ThrAla: 4.343 ± 0.121	0.365ThrCys: 0.365 ± 0.019	3.147ThrAsp: 3.147 ± 0.079	3.116ThrGlu: 3.116 ± 0.06	2.933ThrPhe: 2.933 ± 0.059	4.386ThrGly: 4.386 ± 0.116	1.03ThrHis: 1.03 ± 0.034	4.39ThrIle: 4.39 ± 0.077	3.659ThrLys: 3.659 ± 0.059	5.372ThrLeu: 5.372 ± 0.078	0.861ThrMet: 0.861 ± 0.029	3.283ThrAsn: 3.283 ± 0.075	2.383ThrPro: 2.383 ± 0.043	2.108ThrGln: 2.108 ± 0.048	1.527ThrArg: 1.527 ± 0.037	3.831ThrSer: 3.831 ± 0.088	3.325ThrThr: 3.325 ± 0.082	3.675ThrVal: 3.675 ± 0.081	0.557ThrTrp: 0.557 ± 0.024	2.363ThrTyr: 2.363 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.381ValAla: 4.381 ± 0.07	0.522ValCys: 0.522 ± 0.02	3.204ValAsp: 3.204 ± 0.051	3.544ValGlu: 3.544 ± 0.068	3.342ValPhe: 3.342 ± 0.063	3.852ValGly: 3.852 ± 0.078	0.823ValHis: 0.823 ± 0.023	4.817ValIle: 4.817 ± 0.065	4.921ValLys: 4.921 ± 0.065	5.661ValLeu: 5.661 ± 0.081	1.331ValMet: 1.331 ± 0.034	3.939ValAsn: 3.939 ± 0.065	2.018ValPro: 2.018 ± 0.047	1.709ValGln: 1.709 ± 0.041	1.862ValArg: 1.862 ± 0.044	4.393ValSer: 4.393 ± 0.069	3.487ValThr: 3.487 ± 0.083	4.014ValVal: 4.014 ± 0.071	0.643ValTrp: 0.643 ± 0.024	2.402ValTyr: 2.402 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.668TrpAla: 0.668 ± 0.024	0.103TrpCys: 0.103 ± 0.009	0.631TrpAsp: 0.631 ± 0.029	0.691TrpGlu: 0.691 ± 0.027	0.555TrpPhe: 0.555 ± 0.023	0.736TrpGly: 0.736 ± 0.027	0.212TrpHis: 0.212 ± 0.012	0.752TrpIle: 0.752 ± 0.024	0.817TrpLys: 0.817 ± 0.033	1.082TrpLeu: 1.082 ± 0.037	0.304TrpMet: 0.304 ± 0.015	0.679TrpAsn: 0.679 ± 0.022	0.299TrpPro: 0.299 ± 0.016	0.419TrpGln: 0.419 ± 0.018	0.438TrpArg: 0.438 ± 0.022	0.646TrpSer: 0.646 ± 0.023	0.58TrpThr: 0.58 ± 0.025	0.643TrpVal: 0.643 ± 0.026	0.157TrpTrp: 0.157 ± 0.012	0.443TrpTyr: 0.443 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.608TyrAla: 2.608 ± 0.048	0.337TyrCys: 0.337 ± 0.019	2.127TyrAsp: 2.127 ± 0.045	1.991TyrGlu: 1.991 ± 0.045	2.431TyrPhe: 2.431 ± 0.048	2.647TyrGly: 2.647 ± 0.06	0.86TyrHis: 0.86 ± 0.029	2.61TyrIle: 2.61 ± 0.052	2.826TyrLys: 2.826 ± 0.05	4.094TyrLeu: 4.094 ± 0.069	0.626TyrMet: 0.626 ± 0.024	2.294TyrAsn: 2.294 ± 0.047	1.579TyrPro: 1.579 ± 0.041	2.031TyrGln: 2.031 ± 0.048	1.56TyrArg: 1.56 ± 0.041	2.667TyrSer: 2.667 ± 0.054	2.333TyrThr: 2.333 ± 0.055	1.996TyrVal: 1.996 ± 0.04	0.484TyrTrp: 0.484 ± 0.023	1.715TyrTyr: 1.715 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3357 proteins (1173386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---