Mycobacterium virus Pacc40
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5U5E0|B5U5E0_9CAUD Uncharacterized protein OS=Mycobacterium virus Pacc40 OX=563122 GN=94 PE=4 SV=1
MM1 pKa = 7.61 TDD3 pKa = 3.76 DD4 pKa = 4.64 EE5 pKa = 4.56 KK6 pKa = 10.83 TEE8 pKa = 3.98 QLRR11 pKa = 11.84 KK12 pKa = 9.31 EE13 pKa = 4.18 AFASLEE19 pKa = 3.79 DD20 pKa = 4.36 TIAKK24 pKa = 9.78 FADD27 pKa = 3.73 LQNQHH32 pKa = 6.16 NAVTDD37 pKa = 3.81 TGAEE41 pKa = 4.06 MAVDD45 pKa = 4.14 AVLLIGMQYY54 pKa = 11.04 LDD56 pKa = 4.8 DD57 pKa = 5.46 DD58 pKa = 4.84 GDD60 pKa = 3.55 RR61 pKa = 11.84 GGYY64 pKa = 9.47 VNIIPRR70 pKa = 11.84 NGWQPGYY77 pKa = 8.74 ITAGLLSMAQATVVEE92 pKa = 4.51 SHH94 pKa = 6.17 TCSGQDD100 pKa = 3.52 DD101 pKa = 3.85
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.172
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.732
ProMoST 4.05
Dawson 3.948
Bjellqvist 4.151
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.304
Thurlkill 3.795
EMBOSS 3.897
Sillero 4.075
Patrickios 3.478
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|B5U5A8|B5U5A8_9CAUD Uncharacterized protein OS=Mycobacterium virus Pacc40 OX=563122 GN=62 PE=4 SV=1
MM1 pKa = 7.01 TAEE4 pKa = 3.81 SMLWFRR10 pKa = 11.84 ARR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 SHH16 pKa = 6.55 RR17 pKa = 11.84 SAWGHH22 pKa = 5.8 PRR24 pKa = 11.84 PPAPPKK30 pKa = 8.81 PQPITGEE37 pKa = 4.07 PMSNLTPEE45 pKa = 4.02 QLEE48 pKa = 4.41 AIAYY52 pKa = 8.8 IVLAFTGPPSLAYY65 pKa = 10.09 FLVKK69 pKa = 10.51 GLFRR73 pKa = 6.02
Molecular weight: 8.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.911
IPC_protein 10.994
Toseland 10.95
ProMoST 11.477
Dawson 11.023
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.008
Grimsley 11.082
Solomon 11.301
Lehninger 11.242
Nozaki 10.935
DTASelect 10.862
Thurlkill 10.95
EMBOSS 11.389
Sillero 10.979
Patrickios 10.833
IPC_peptide 11.301
IPC2_peptide 10.058
IPC2.peptide.svr19 8.826
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
101
0
101
18797
30
1341
186.1
20.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.177 ± 0.457
1.213 ± 0.184
6.645 ± 0.237
5.958 ± 0.27
2.947 ± 0.197
9.033 ± 0.551
2.304 ± 0.216
4.315 ± 0.209
3.384 ± 0.185
7.379 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.122
3.458 ± 0.167
6.044 ± 0.228
3.596 ± 0.208
6.847 ± 0.405
5.942 ± 0.26
6.506 ± 0.287
7.326 ± 0.266
2.288 ± 0.158
2.458 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here