Salmonella phage SETP7
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5N149|U5N149_9CAUD Uncharacterized protein OS=Salmonella phage SETP7 OX=424947 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.39 VYY4 pKa = 10.14 IVSGWHH10 pKa = 6.51 HH11 pKa = 6.5 YY12 pKa = 10.56 EE13 pKa = 3.66 DD14 pKa = 4.42 TYY16 pKa = 11.86 VLGVYY21 pKa = 10.59 LSEE24 pKa = 4.44 EE25 pKa = 4.09 KK26 pKa = 11.09 AEE28 pKa = 4.11 AAAEE32 pKa = 4.13 LEE34 pKa = 4.59 RR35 pKa = 11.84 TSHH38 pKa = 6.36 YY39 pKa = 10.68 YY40 pKa = 10.74 DD41 pKa = 3.79 GVDD44 pKa = 3.45 VLGWEE49 pKa = 4.15 AQEE52 pKa = 4.0
Molecular weight: 6.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.306
IPC2_protein 4.52
IPC_protein 4.342
Toseland 4.215
ProMoST 4.355
Dawson 4.279
Bjellqvist 4.52
Wikipedia 4.139
Rodwell 4.19
Grimsley 4.126
Solomon 4.266
Lehninger 4.228
Nozaki 4.406
DTASelect 4.482
Thurlkill 4.215
EMBOSS 4.164
Sillero 4.457
Patrickios 0.54
IPC_peptide 4.279
IPC2_peptide 4.444
IPC2.peptide.svr19 4.435
Protein with the highest isoelectric point:
>tr|U5N160|U5N160_9CAUD Uncharacterized protein OS=Salmonella phage SETP7 OX=424947 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.34 PNDD5 pKa = 3.45 LVTWTGRR12 pKa = 11.84 NGEE15 pKa = 3.98 IRR17 pKa = 11.84 HH18 pKa = 5.75 GKK20 pKa = 9.57 VISLHH25 pKa = 6.12 GIYY28 pKa = 10.48 ARR30 pKa = 11.84 VEE32 pKa = 3.5 WCRR35 pKa = 11.84 AHH37 pKa = 6.43 SKK39 pKa = 9.55 KK40 pKa = 9.94 PRR42 pKa = 11.84 YY43 pKa = 8.05 ITVRR47 pKa = 11.84 QDD49 pKa = 3.14 KK50 pKa = 10.78 LIVKK54 pKa = 9.6
Molecular weight: 6.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.736
IPC_protein 10.189
Toseland 10.701
ProMoST 10.365
Dawson 10.804
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.228
Grimsley 10.833
Solomon 10.877
Lehninger 10.847
Nozaki 10.687
DTASelect 10.438
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.994
IPC_peptide 10.877
IPC2_peptide 9.238
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
13283
39
852
198.3
22.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.448 ± 0.541
0.949 ± 0.114
6.264 ± 0.173
6.693 ± 0.393
3.712 ± 0.169
7.724 ± 0.308
1.769 ± 0.16
4.916 ± 0.168
5.97 ± 0.328
7.814 ± 0.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.567 ± 0.154
4.366 ± 0.208
3.824 ± 0.201
3.606 ± 0.232
5.729 ± 0.222
5.932 ± 0.28
6.339 ± 0.324
7.468 ± 0.309
1.476 ± 0.144
3.433 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here