Acetanaerobacterium elongatum
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2758 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9U2Q3|A0A1G9U2Q3_9FIRM X-X-X-Leu-X-X-Gly heptad repeat-containing protein OS=Acetanaerobacterium elongatum OX=258515 GN=SAMN05192585_10155 PE=4 SV=1
MM1 pKa = 7.43 NNLVFNTTAAEE12 pKa = 4.26 LKK14 pKa = 9.61 TSVYY18 pKa = 9.96 AQKK21 pKa = 10.89 SDD23 pKa = 3.68 DD24 pKa = 3.91 SFAAIQIDD32 pKa = 3.69 NSGNLYY38 pKa = 10.92 VNVSNTSLTVAGSVTINNTDD58 pKa = 3.27 LSIAGDD64 pKa = 3.57 VTITNTSLTVAGSVTINNTDD84 pKa = 3.31 LSVAGDD90 pKa = 3.53 VTITNTSLTVAGSVTVNNATITTFVNGSRR119 pKa = 11.84 LTTITLSSSAVTSNTILLDD138 pKa = 3.67 NSDD141 pKa = 2.67 ISQYY145 pKa = 11.16 KK146 pKa = 8.81 NAAFMLYY153 pKa = 10.18 NEE155 pKa = 4.42 STATLVTLNLQVSPTDD171 pKa = 3.24 TTTDD175 pKa = 3.91 YY176 pKa = 10.48 ITDD179 pKa = 3.75 TTLGTVTLAANGKK192 pKa = 9.7 LYY194 pKa = 10.52 IPVSVVGKK202 pKa = 8.93 NVRR205 pKa = 11.84 LLATSASDD213 pKa = 3.32 TVTVNGYY220 pKa = 9.47 YY221 pKa = 9.94 VAQAA225 pKa = 3.47
Molecular weight: 23.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.656
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.567
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.342
Thurlkill 3.732
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.912
Protein with the highest isoelectric point:
>tr|A0A1H0GZT3|A0A1H0GZT3_9FIRM Transcriptional regulator LacI family OS=Acetanaerobacterium elongatum OX=258515 GN=SAMN05192585_15810 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 4.87 LQKK5 pKa = 10.84 PSIRR9 pKa = 11.84 LLYY12 pKa = 10.12 AWWLALFCATLVLCCAAASLLSFSRR37 pKa = 11.84 PLWVWVTVVLLAVFSAGFIWVLPQRR62 pKa = 11.84 YY63 pKa = 8.45 RR64 pKa = 11.84 RR65 pKa = 11.84 LAYY68 pKa = 9.54 GVKK71 pKa = 10.1 GSLVMVRR78 pKa = 11.84 CGIIGVRR85 pKa = 11.84 EE86 pKa = 3.91 KK87 pKa = 11.26 YY88 pKa = 10.36 ILVEE92 pKa = 4.22 NIRR95 pKa = 11.84 FASMDD100 pKa = 3.1 ATLPLAAFGLATATLRR116 pKa = 11.84 TAGATLRR123 pKa = 11.84 IPGLDD128 pKa = 3.14 ADD130 pKa = 3.77 AQKK133 pKa = 10.91 RR134 pKa = 11.84 LKK136 pKa = 10.55 AQLSPSFGRR145 pKa = 3.98
Molecular weight: 16.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.335
Toseland 10.379
ProMoST 10.335
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.774
Grimsley 10.628
Solomon 10.628
Lehninger 10.599
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.423
EMBOSS 10.789
Sillero 10.482
Patrickios 10.496
IPC_peptide 10.628
IPC2_peptide 9.545
IPC2.peptide.svr19 8.512
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2758
0
2758
829626
24
2639
300.8
33.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.779 ± 0.062
1.505 ± 0.021
5.156 ± 0.036
6.351 ± 0.05
4.106 ± 0.038
7.213 ± 0.051
1.597 ± 0.02
7.099 ± 0.044
6.229 ± 0.041
9.661 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.024
4.361 ± 0.031
3.751 ± 0.031
3.305 ± 0.025
4.475 ± 0.039
6.328 ± 0.053
5.83 ± 0.054
7.056 ± 0.038
0.8 ± 0.016
3.773 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here