Acetanaerobacterium elongatum 
Average proteome isoelectric point is 6.37 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2758 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1G9U2Q3|A0A1G9U2Q3_9FIRM X-X-X-Leu-X-X-Gly heptad repeat-containing protein OS=Acetanaerobacterium elongatum OX=258515 GN=SAMN05192585_10155 PE=4 SV=1MM1 pKa = 7.43  NNLVFNTTAAEE12 pKa = 4.26  LKK14 pKa = 9.61  TSVYY18 pKa = 9.96  AQKK21 pKa = 10.89  SDD23 pKa = 3.68  DD24 pKa = 3.91  SFAAIQIDD32 pKa = 3.69  NSGNLYY38 pKa = 10.92  VNVSNTSLTVAGSVTINNTDD58 pKa = 3.27  LSIAGDD64 pKa = 3.57  VTITNTSLTVAGSVTINNTDD84 pKa = 3.31  LSVAGDD90 pKa = 3.53  VTITNTSLTVAGSVTVNNATITTFVNGSRR119 pKa = 11.84  LTTITLSSSAVTSNTILLDD138 pKa = 3.67  NSDD141 pKa = 2.67  ISQYY145 pKa = 11.16  KK146 pKa = 8.81  NAAFMLYY153 pKa = 10.18  NEE155 pKa = 4.42  STATLVTLNLQVSPTDD171 pKa = 3.24  TTTDD175 pKa = 3.91  YY176 pKa = 10.48  ITDD179 pKa = 3.75  TTLGTVTLAANGKK192 pKa = 9.7  LYY194 pKa = 10.52  IPVSVVGKK202 pKa = 8.93  NVRR205 pKa = 11.84  LLATSASDD213 pKa = 3.32  TVTVNGYY220 pKa = 9.47  YY221 pKa = 9.94  VAQAA225 pKa = 3.47  
 23.32 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.741 
IPC2_protein 3.91 
IPC_protein 3.884 
Toseland    3.656 
ProMoST     4.088 
Dawson      3.91 
Bjellqvist  4.075 
Wikipedia   3.91 
Rodwell     3.719 
Grimsley    3.567 
Solomon     3.897 
Lehninger   3.859 
Nozaki      4.037 
DTASelect   4.342 
Thurlkill   3.732 
EMBOSS      3.91 
Sillero     4.024 
Patrickios  1.952 
IPC_peptide 3.884 
IPC2_peptide  3.986 
IPC2.peptide.svr19  3.912 
 Protein with the highest isoelectric point: 
>tr|A0A1H0GZT3|A0A1H0GZT3_9FIRM Transcriptional regulator  LacI family OS=Acetanaerobacterium elongatum OX=258515 GN=SAMN05192585_15810 PE=4 SV=1MM1 pKa = 7.67  EE2 pKa = 4.87  LQKK5 pKa = 10.84  PSIRR9 pKa = 11.84  LLYY12 pKa = 10.12  AWWLALFCATLVLCCAAASLLSFSRR37 pKa = 11.84  PLWVWVTVVLLAVFSAGFIWVLPQRR62 pKa = 11.84  YY63 pKa = 8.45  RR64 pKa = 11.84  RR65 pKa = 11.84  LAYY68 pKa = 9.54  GVKK71 pKa = 10.1  GSLVMVRR78 pKa = 11.84  CGIIGVRR85 pKa = 11.84  EE86 pKa = 3.91  KK87 pKa = 11.26  YY88 pKa = 10.36  ILVEE92 pKa = 4.22  NIRR95 pKa = 11.84  FASMDD100 pKa = 3.1  ATLPLAAFGLATATLRR116 pKa = 11.84  TAGATLRR123 pKa = 11.84  IPGLDD128 pKa = 3.14  ADD130 pKa = 3.77  AQKK133 pKa = 10.91  RR134 pKa = 11.84  LKK136 pKa = 10.55  AQLSPSFGRR145 pKa = 3.98  
 16.02 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.349 
IPC2_protein 9.633 
IPC_protein 10.335 
Toseland    10.379 
ProMoST     10.335 
Dawson      10.555 
Bjellqvist  10.292 
Wikipedia   10.76 
Rodwell     10.774 
Grimsley    10.628 
Solomon     10.628 
Lehninger   10.599 
Nozaki      10.438 
DTASelect   10.262 
Thurlkill   10.423 
EMBOSS      10.789 
Sillero     10.482 
Patrickios  10.496 
IPC_peptide 10.628 
IPC2_peptide  9.545 
IPC2.peptide.svr19  8.512 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2758 
0
2758 
829626
24
2639
300.8
33.27
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.779 ± 0.062
1.505 ± 0.021
5.156 ± 0.036
6.351 ± 0.05
4.106 ± 0.038
7.213 ± 0.051
1.597 ± 0.02
7.099 ± 0.044
6.229 ± 0.041
9.661 ± 0.063
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.623 ± 0.024
4.361 ± 0.031
3.751 ± 0.031
3.305 ± 0.025
4.475 ± 0.039
6.328 ± 0.053
5.83 ± 0.054
7.056 ± 0.038
0.8 ± 0.016
3.773 ± 0.029
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here