Kaumoebavirus
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0CNX9|A0A1V0CNX9_9VIRU Uncharacterized protein OS=Kaumoebavirus OX=1859492 GN=BNJ_00223 PE=4 SV=1
MM1 pKa = 7.53 HH2 pKa = 7.81 PLEE5 pKa = 4.17 QIFRR9 pKa = 11.84 NFLEE13 pKa = 4.31 SLEE16 pKa = 4.35 KK17 pKa = 10.4 AWLEE21 pKa = 3.82 LDD23 pKa = 3.87 HH24 pKa = 7.46 VDD26 pKa = 4.27 TEE28 pKa = 4.48 VEE30 pKa = 4.15 YY31 pKa = 11.38 AEE33 pKa = 5.14 SNTSRR38 pKa = 11.84 YY39 pKa = 8.96 ARR41 pKa = 11.84 VMYY44 pKa = 10.44 RR45 pKa = 11.84 FADD48 pKa = 3.6 EE49 pKa = 5.32 FIVEE53 pKa = 5.42 GIFEE57 pKa = 4.68 LCGQYY62 pKa = 9.14 MEE64 pKa = 5.58 ASLLSCHH71 pKa = 6.73 GEE73 pKa = 3.99 LFRR76 pKa = 11.84 WQYY79 pKa = 11.25 DD80 pKa = 3.13 ISADD84 pKa = 3.4 PLLIHH89 pKa = 6.48 VAYY92 pKa = 10.1 ADD94 pKa = 2.99 IMEE97 pKa = 4.73 YY98 pKa = 10.12 IEE100 pKa = 5.47 DD101 pKa = 3.75 NYY103 pKa = 11.37 NALFQEE109 pKa = 5.09 DD110 pKa = 3.88 EE111 pKa = 4.44 DD112 pKa = 5.33 ADD114 pKa = 4.08 SEE116 pKa = 4.56 LEE118 pKa = 4.1 PEE120 pKa = 4.17 QGIAPGPFTSVFEE133 pKa = 4.58 NVAHH137 pKa = 6.49 MLTVV141 pKa = 3.41
Molecular weight: 16.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.87
IPC2_protein 4.05
IPC_protein 3.973
Toseland 3.808
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.795
Rodwell 3.808
Grimsley 3.719
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.164
Thurlkill 3.834
EMBOSS 3.821
Sillero 4.088
Patrickios 0.604
IPC_peptide 3.923
IPC2_peptide 4.075
IPC2.peptide.svr19 3.961
Protein with the highest isoelectric point:
>tr|A0A1V0CNC5|A0A1V0CNC5_9VIRU Uncharacterized protein OS=Kaumoebavirus OX=1859492 GN=BNJ_00009 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 YY3 pKa = 9.03 VRR5 pKa = 11.84 GMFKK9 pKa = 10.46 YY10 pKa = 9.12 VWCLAAPATRR20 pKa = 11.84 PKK22 pKa = 10.35 RR23 pKa = 11.84 DD24 pKa = 3.13 FFGGVRR30 pKa = 11.84 GILRR34 pKa = 11.84 RR35 pKa = 11.84 NYY37 pKa = 9.71 GCEE40 pKa = 3.93 DD41 pKa = 3.52 EE42 pKa = 5.92 LRR44 pKa = 11.84 EE45 pKa = 4.1 QKK47 pKa = 10.19 RR48 pKa = 11.84 GKK50 pKa = 9.84 ALKK53 pKa = 10.4 NVV55 pKa = 3.39
Molecular weight: 6.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.599
ProMoST 10.277
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.76
IPC_peptide 10.818
IPC2_peptide 9.516
IPC2.peptide.svr19 8.465
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
427
0
427
93067
37
1589
218.0
25.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.106 ± 0.132
1.62 ± 0.074
5.879 ± 0.102
7.624 ± 0.155
4.501 ± 0.089
5.551 ± 0.136
1.963 ± 0.057
6.956 ± 0.107
7.996 ± 0.161
8.634 ± 0.116
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.613 ± 0.059
5.047 ± 0.096
3.847 ± 0.086
2.872 ± 0.076
5.247 ± 0.112
6.068 ± 0.122
5.228 ± 0.147
6.192 ± 0.092
1.318 ± 0.053
4.737 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here