Gibbsiella quercinecans
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4735 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A250B2H6|A0A250B2H6_9GAMM ABC transporter OS=Gibbsiella quercinecans OX=929813 GN=AWC35_14430 PE=3 SV=1
MM1 pKa = 7.31 QMVIIHH7 pKa = 6.19 HH8 pKa = 6.65 WGEE11 pKa = 4.11 GMLNTLNNINKK22 pKa = 8.36 EE23 pKa = 4.21 LCDD26 pKa = 4.75 LDD28 pKa = 5.99 CPDD31 pKa = 5.77 DD32 pKa = 5.68 DD33 pKa = 5.02 GADD36 pKa = 3.57 DD37 pKa = 3.7 EE38 pKa = 5.01 TINRR42 pKa = 11.84 NIMIVSLIMSGVLLEE57 pKa = 4.11 YY58 pKa = 10.26 SIYY61 pKa = 9.7 MCC63 pKa = 5.71
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 3.91
IPC_protein 3.808
Toseland 3.617
ProMoST 4.012
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 0.401
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A250AWX5|A0A250AWX5_9GAMM ABC transporter domain-containing protein OS=Gibbsiella quercinecans OX=929813 GN=AWC35_01885 PE=4 SV=1
MM1 pKa = 7.41 VKK3 pKa = 10.19 PFSLTAGIFPYY14 pKa = 10.3 LNRR17 pKa = 11.84 MWRR20 pKa = 11.84 LVMTGCCFILFALGGLWLSLGWMNLLRR47 pKa = 11.84 VVQPDD52 pKa = 2.87 KK53 pKa = 10.96 RR54 pKa = 11.84 KK55 pKa = 9.8 RR56 pKa = 11.84 RR57 pKa = 11.84 QQARR61 pKa = 11.84 RR62 pKa = 11.84 SIATSFRR69 pKa = 11.84 FFLSCARR76 pKa = 11.84 ATGVLDD82 pKa = 3.47 YY83 pKa = 10.54 RR84 pKa = 11.84 IEE86 pKa = 4.2 GLEE89 pKa = 3.79 TLQRR93 pKa = 11.84 EE94 pKa = 4.38 RR95 pKa = 11.84 GCLVVANHH103 pKa = 6.57 PTLIDD108 pKa = 3.72 YY109 pKa = 10.62 VLLASVMPEE118 pKa = 3.51 VDD120 pKa = 4.16 CLVKK124 pKa = 10.67 ADD126 pKa = 3.8 LQRR129 pKa = 11.84 NIFFRR134 pKa = 11.84 GVIRR138 pKa = 11.84 AADD141 pKa = 3.69 YY142 pKa = 10.83 LINSQADD149 pKa = 3.67 SLLPACRR156 pKa = 11.84 QRR158 pKa = 11.84 LAQGDD163 pKa = 4.08 VILIFPEE170 pKa = 4.63 GTRR173 pKa = 11.84 TRR175 pKa = 11.84 PGQPLALQRR184 pKa = 11.84 GAANIAVRR192 pKa = 11.84 CGCDD196 pKa = 3.13 LRR198 pKa = 11.84 VVHH201 pKa = 6.69 IRR203 pKa = 11.84 CSQQTLGKK211 pKa = 9.62 HH212 pKa = 4.86 SRR214 pKa = 11.84 WYY216 pKa = 10.15 QIPNEE221 pKa = 4.32 KK222 pKa = 10.07 PLFTLTVTQRR232 pKa = 11.84 VSSASFITSHH242 pKa = 7.43 DD243 pKa = 4.08 ALPLAARR250 pKa = 11.84 RR251 pKa = 11.84 LNRR254 pKa = 11.84 FLQHH258 pKa = 6.92 ALTPEE263 pKa = 4.07 HH264 pKa = 6.88
Molecular weight: 29.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.472
IPC_protein 10.394
Toseland 10.423
ProMoST 10.233
Dawson 10.57
Bjellqvist 10.365
Wikipedia 10.818
Rodwell 10.628
Grimsley 10.643
Solomon 10.701
Lehninger 10.657
Nozaki 10.511
DTASelect 10.335
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.526
Patrickios 10.292
IPC_peptide 10.701
IPC2_peptide 9.838
IPC2.peptide.svr19 8.465
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4735
0
4735
1558740
28
3317
329.2
36.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.72 ± 0.042
1.058 ± 0.012
4.987 ± 0.025
5.231 ± 0.032
3.792 ± 0.024
7.643 ± 0.03
2.282 ± 0.018
5.719 ± 0.033
3.776 ± 0.033
11.229 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.584 ± 0.016
3.56 ± 0.026
4.68 ± 0.023
4.906 ± 0.036
5.816 ± 0.028
5.702 ± 0.03
5.031 ± 0.028
6.975 ± 0.029
1.436 ± 0.019
2.873 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here