Ephemeroptericola cinctiostellae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Ephemeroptericola

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2476 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345DBY0|A0A345DBY0_9BURK Uncharacterized protein OS=Ephemeroptericola cinctiostellae OX=2268024 GN=DTO96_101608 PE=4 SV=1
MM1 pKa = 7.5IVLGTNNADD10 pKa = 3.75TLGSSGDD17 pKa = 3.53DD18 pKa = 3.45TLIGLAGDD26 pKa = 3.88DD27 pKa = 4.16TLNGYY32 pKa = 8.75LPWGTALGNTTYY44 pKa = 10.74RR45 pKa = 11.84FSKK48 pKa = 10.89GDD50 pKa = 3.33GNDD53 pKa = 3.3VVKK56 pKa = 10.8EE57 pKa = 4.02NAGSDD62 pKa = 3.64TIEE65 pKa = 4.06FTDD68 pKa = 3.64VKK70 pKa = 9.84STEE73 pKa = 4.06VFFKK77 pKa = 10.88LGNNGKK83 pKa = 9.52DD84 pKa = 3.54LIILYY89 pKa = 7.61GTNNSITISEE99 pKa = 4.23MIYY102 pKa = 9.31WPSGRR107 pKa = 11.84AIEE110 pKa = 4.15NFKK113 pKa = 11.07FSDD116 pKa = 3.94GVIWDD121 pKa = 3.95LNEE124 pKa = 4.71LYY126 pKa = 11.04NNTATTIGTLGNDD139 pKa = 4.76IISGWDD145 pKa = 3.34GFNVIDD151 pKa = 4.88GKK153 pKa = 11.24EE154 pKa = 4.07GDD156 pKa = 4.51DD157 pKa = 5.44LINGQTKK164 pKa = 10.6NDD166 pKa = 3.82TLLGGLGNDD175 pKa = 3.87TLYY178 pKa = 11.27GGYY181 pKa = 10.83GNDD184 pKa = 4.2ASDD187 pKa = 4.47GGDD190 pKa = 3.48GDD192 pKa = 5.37DD193 pKa = 4.18YY194 pKa = 11.64LYY196 pKa = 11.08DD197 pKa = 3.51SSGNNTLLGGAGNDD211 pKa = 3.53SLYY214 pKa = 11.23GGAGNDD220 pKa = 3.57VLNGGDD226 pKa = 3.82GNDD229 pKa = 3.44VLFGGDD235 pKa = 3.86GNDD238 pKa = 2.67IYY240 pKa = 11.12YY241 pKa = 10.2INDD244 pKa = 3.41IGDD247 pKa = 3.48QIYY250 pKa = 10.62DD251 pKa = 3.61SNGIADD257 pKa = 3.72TAYY260 pKa = 9.58ISVNGVRR267 pKa = 11.84TTGIEE272 pKa = 3.89NTVYY276 pKa = 10.88TNDD279 pKa = 3.93AQPLPYY285 pKa = 10.12FIRR288 pKa = 11.84ALDD291 pKa = 3.64SKK293 pKa = 7.9YY294 pKa = 8.08TWGAIGQGQTLNYY307 pKa = 10.32SFALNADD314 pKa = 3.41GGEE317 pKa = 4.31VGFEE321 pKa = 4.68LYY323 pKa = 10.14TEE325 pKa = 4.17EE326 pKa = 3.86QKK328 pKa = 9.8TAIRR332 pKa = 11.84EE333 pKa = 4.14ALQKK337 pKa = 10.37YY338 pKa = 8.63AQLVNLTFVEE348 pKa = 4.93VVDD351 pKa = 4.0STSVQLRR358 pKa = 11.84FFRR361 pKa = 11.84DD362 pKa = 3.56DD363 pKa = 3.85LSTAGYY369 pKa = 9.49AAYY372 pKa = 9.91AGYY375 pKa = 11.18ANYY378 pKa = 9.94PFDD381 pKa = 3.8SSKK384 pKa = 11.36GGDD387 pKa = 2.93IHH389 pKa = 7.72IKK391 pKa = 8.82NTISNLNQLDD401 pKa = 3.64GSFDD405 pKa = 3.74VLIHH409 pKa = 6.6EE410 pKa = 5.04IGHH413 pKa = 6.26ALGLKK418 pKa = 10.24HH419 pKa = 6.57PFEE422 pKa = 5.07DD423 pKa = 4.07TDD425 pKa = 3.91TLPTSEE431 pKa = 6.04DD432 pKa = 3.48STTYY436 pKa = 11.12SAMSYY441 pKa = 10.59NSLWQANKK449 pKa = 8.67NTVRR453 pKa = 11.84MYY455 pKa = 11.38DD456 pKa = 3.65LATLHH461 pKa = 6.09YY462 pKa = 9.38FYY464 pKa = 11.04GVNASQRR471 pKa = 11.84SEE473 pKa = 3.8NNTYY477 pKa = 11.08AFSDD481 pKa = 3.72ANFTDD486 pKa = 3.95HH487 pKa = 6.65YY488 pKa = 9.3VWDD491 pKa = 4.19GAGVDD496 pKa = 3.77VFDD499 pKa = 6.52ANSQTQNVSVNLNPGSWNFIGSKK522 pKa = 10.07AVSILSAGQSFIGFGTLIEE541 pKa = 4.25NAVSGSGNDD550 pKa = 3.67TVIGNALDD558 pKa = 3.97NQIHH562 pKa = 5.93TNAGNDD568 pKa = 3.49WLKK571 pKa = 11.29GGAGNDD577 pKa = 3.18SLYY580 pKa = 11.04AGEE583 pKa = 5.88GNDD586 pKa = 3.74TLNGQVGADD595 pKa = 3.42VMVGGNGDD603 pKa = 3.44DD604 pKa = 3.94YY605 pKa = 11.63YY606 pKa = 11.84YY607 pKa = 10.89FDD609 pKa = 3.9NVGDD613 pKa = 3.91AVIEE617 pKa = 4.23VVDD620 pKa = 4.23AGLDD624 pKa = 3.75CVEE627 pKa = 4.93ASANCTLNDD636 pKa = 3.79NIEE639 pKa = 4.02NLVLSGSGALSGMGNALNNQMKK661 pKa = 10.52GSAEE665 pKa = 4.0NNTLKK670 pKa = 10.82GMAGNDD676 pKa = 3.52SLLGFAGNDD685 pKa = 3.45ALDD688 pKa = 4.11GGTGNDD694 pKa = 3.63TLRR697 pKa = 11.84GGLGNDD703 pKa = 3.52VLNGGDD709 pKa = 3.82GIDD712 pKa = 3.28WADD715 pKa = 3.82YY716 pKa = 11.1VDD718 pKa = 3.89MTASVNVNLNITTVQVTGAGGSDD741 pKa = 3.08TLTNIEE747 pKa = 4.08NVRR750 pKa = 11.84AGSANDD756 pKa = 3.54NLKK759 pKa = 11.11GNASDD764 pKa = 4.61NVLDD768 pKa = 4.29GQAGNDD774 pKa = 3.48ALFGNAGNDD783 pKa = 3.55NLIGGAGNDD792 pKa = 3.6ILNGGLGNDD801 pKa = 3.86NLSGGDD807 pKa = 3.57GNDD810 pKa = 2.99WADD813 pKa = 3.78YY814 pKa = 10.22FDD816 pKa = 3.51STAAVSVDD824 pKa = 3.57LRR826 pKa = 11.84LATQQNTVGAGLDD839 pKa = 2.89IFYY842 pKa = 10.52NIEE845 pKa = 3.91NLRR848 pKa = 11.84GGLGNDD854 pKa = 3.6TLNGSVYY861 pKa = 11.12ANALVGQAGNDD872 pKa = 3.35FMVGNGGNDD881 pKa = 3.12TLYY884 pKa = 11.05AGLGNDD890 pKa = 3.71TLFGGTGNDD899 pKa = 2.84TYY901 pKa = 11.38QFNRR905 pKa = 11.84GDD907 pKa = 3.93GQDD910 pKa = 3.41TLTDD914 pKa = 3.4ADD916 pKa = 3.79ATVGNSDD923 pKa = 3.47TLAFQTGVTNSQLWLTHH940 pKa = 5.94TGNDD944 pKa = 3.35LVVSVIGTTDD954 pKa = 2.87KK955 pKa = 10.7VTIKK959 pKa = 10.26NWYY962 pKa = 8.22VSSANHH968 pKa = 5.79VEE970 pKa = 4.34SITAGGKK977 pKa = 7.53TLADD981 pKa = 3.73GNVNALVAAMSAFSPPAAGQTTLPSSYY1008 pKa = 8.48QTALNPVIAANWVV1021 pKa = 3.13

Molecular weight: 106.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345DDD9|A0A345DDD9_9BURK 2-aminoadipate transaminase OS=Ephemeroptericola cinctiostellae OX=2268024 GN=lysN_2 PE=4 SV=1
MM1 pKa = 7.6AAPRR5 pKa = 11.84SKK7 pKa = 9.47TGRR10 pKa = 11.84PEE12 pKa = 3.46RR13 pKa = 11.84RR14 pKa = 11.84PAQNPLFKK22 pKa = 10.35RR23 pKa = 11.84KK24 pKa = 8.99RR25 pKa = 11.84VCRR28 pKa = 11.84FTMAKK33 pKa = 9.68VDD35 pKa = 3.74QIDD38 pKa = 3.91YY39 pKa = 11.04KK40 pKa = 11.33DD41 pKa = 3.47VDD43 pKa = 3.83TLKK46 pKa = 11.18DD47 pKa = 4.79FIAEE51 pKa = 3.96NGKK54 pKa = 10.03IIPARR59 pKa = 11.84LTGTRR64 pKa = 11.84AIYY67 pKa = 9.74QRR69 pKa = 11.84QLNTCIKK76 pKa = 9.93RR77 pKa = 11.84ARR79 pKa = 11.84FLALVPYY86 pKa = 9.78CDD88 pKa = 3.37NHH90 pKa = 6.44

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2476

0

2476

776007

30

4882

313.4

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.571 ± 0.069

0.973 ± 0.018

5.556 ± 0.052

5.439 ± 0.106

3.901 ± 0.041

7.117 ± 0.082

2.665 ± 0.04

5.573 ± 0.043

4.6 ± 0.045

10.085 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.864 ± 0.031

4.042 ± 0.04

4.228 ± 0.039

4.106 ± 0.049

4.838 ± 0.056

5.842 ± 0.047

5.729 ± 0.047

7.875 ± 0.127

1.347 ± 0.026

2.651 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski