Bean white chlorosis mosaic virus
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7PRX3|K7PRX3_9GEMI Capsid protein OS=Bean white chlorosis mosaic virus OX=2169732 GN=AV1 PE=3 SV=1
MM1 pKa = 7.88 PPPKK5 pKa = 10.09 KK6 pKa = 10.36 FRR8 pKa = 11.84 VQSKK12 pKa = 10.58 NYY14 pKa = 9.23 FLTYY18 pKa = 8.52 PQCSLTKK25 pKa = 10.54 EE26 pKa = 4.03 EE27 pKa = 5.15 ALSQIQNLATPVNKK41 pKa = 10.09 KK42 pKa = 9.95 FIKK45 pKa = 9.53 ICRR48 pKa = 11.84 EE49 pKa = 3.69 FHH51 pKa = 7.56 EE52 pKa = 5.64 DD53 pKa = 3.65 GQPHH57 pKa = 6.5 LHH59 pKa = 6.28 VLIQFEE65 pKa = 4.74 GKK67 pKa = 8.79 YY68 pKa = 9.48 QCTNNRR74 pKa = 11.84 FFDD77 pKa = 4.02 LVSPTRR83 pKa = 11.84 SAHH86 pKa = 4.86 FHH88 pKa = 6.55 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.57 KK106 pKa = 11.33 DD107 pKa = 3.38 GDD109 pKa = 3.9 TVEE112 pKa = 4.19 WGQFQIDD119 pKa = 3.41 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQSANDD132 pKa = 4.05 TYY134 pKa = 11.82 AKK136 pKa = 10.54 ALNAGSAEE144 pKa = 4.01 AALQIIKK151 pKa = 10.24 EE152 pKa = 4.16 EE153 pKa = 4.13 QPQHH157 pKa = 6.73 FFLQHH162 pKa = 6.07 HH163 pKa = 6.14 NLVANATRR171 pKa = 11.84 IFQKK175 pKa = 10.35 PPEE178 pKa = 4.41 PWTPPFPLSSFTNVPDD194 pKa = 5.1 EE195 pKa = 4.14 MQEE198 pKa = 3.79 WADD201 pKa = 3.9 DD202 pKa = 4.08 YY203 pKa = 11.34 FGKK206 pKa = 10.54 SPAARR211 pKa = 11.84 PEE213 pKa = 4.13 RR214 pKa = 11.84 PLSLIVEE221 pKa = 4.81 GDD223 pKa = 3.34 SRR225 pKa = 11.84 TGKK228 pKa = 8.52 TMWARR233 pKa = 11.84 SLGPHH238 pKa = 6.57 NYY240 pKa = 10.17 LSGHH244 pKa = 6.9 LDD246 pKa = 3.48 FNSRR250 pKa = 11.84 VYY252 pKa = 10.97 SDD254 pKa = 3.42 EE255 pKa = 4.06 VEE257 pKa = 4.31 YY258 pKa = 11.39 NVIDD262 pKa = 4.4 DD263 pKa = 4.14 VAPHH267 pKa = 5.29 YY268 pKa = 10.97 LKK270 pKa = 10.78 LKK272 pKa = 9.03 HH273 pKa = 5.83 WKK275 pKa = 9.59 EE276 pKa = 3.97 LFWAQKK282 pKa = 9.62 KK283 pKa = 6.37 WQSNCKK289 pKa = 8.45 YY290 pKa = 9.81 GKK292 pKa = 9.42 PVVIKK297 pKa = 10.53 GGIPSIVLCNPGEE310 pKa = 4.16 GASYY314 pKa = 11.27 KK315 pKa = 10.87 DD316 pKa = 3.52 FLNKK320 pKa = 10.18 EE321 pKa = 4.14 EE322 pKa = 4.17 NTGIRR327 pKa = 11.84 NWTLKK332 pKa = 10.24 NAVFITLDD340 pKa = 3.28 SPLYY344 pKa = 10.16 QEE346 pKa = 4.51 EE347 pKa = 4.55 TQGGQEE353 pKa = 4.26 EE354 pKa = 4.96 VSQTTPHH361 pKa = 6.32
Molecular weight: 41.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.292
IPC2_protein 6.237
IPC_protein 6.364
Toseland 6.693
ProMoST 6.62
Dawson 6.722
Bjellqvist 6.678
Wikipedia 6.751
Rodwell 6.722
Grimsley 6.839
Solomon 6.737
Lehninger 6.737
Nozaki 6.985
DTASelect 7.161
Thurlkill 7.19
EMBOSS 7.176
Sillero 7.117
Patrickios 4.266
IPC_peptide 6.751
IPC2_peptide 6.883
IPC2.peptide.svr19 6.895
Protein with the highest isoelectric point:
>tr|L7P9S9|L7P9S9_9GEMI Nuclear shuttle protein OS=Bean white chlorosis mosaic virus OX=2169732 GN=BV1 PE=3 SV=1
MM1 pKa = 7.2 YY2 pKa = 9.7 PLRR5 pKa = 11.84 NKK7 pKa = 10.43 RR8 pKa = 11.84 GFTYY12 pKa = 10.33 NPRR15 pKa = 11.84 RR16 pKa = 11.84 FNPRR20 pKa = 11.84 NTAFNRR26 pKa = 11.84 STAGKK31 pKa = 9.13 RR32 pKa = 11.84 HH33 pKa = 5.94 DD34 pKa = 3.61 GKK36 pKa = 10.93 RR37 pKa = 11.84 RR38 pKa = 11.84 GGNALKK44 pKa = 10.78 PNDD47 pKa = 4.26 EE48 pKa = 4.78 PKK50 pKa = 8.3 MTAQRR55 pKa = 11.84 IHH57 pKa = 6.43 EE58 pKa = 4.14 NQYY61 pKa = 9.92 GPEE64 pKa = 3.88 FVMSHH69 pKa = 5.8 NSAISTFISYY79 pKa = 9.76 PSLGKK84 pKa = 8.7 TEE86 pKa = 4.48 PNRR89 pKa = 11.84 SRR91 pKa = 11.84 AYY93 pKa = 9.7 IKK95 pKa = 10.53 LKK97 pKa = 10.15 RR98 pKa = 11.84 LRR100 pKa = 11.84 FKK102 pKa = 10.51 GTVKK106 pKa = 10.13 IEE108 pKa = 3.89 RR109 pKa = 11.84 AQSDD113 pKa = 3.78 MNMDD117 pKa = 3.58 GSIPKK122 pKa = 9.79 VEE124 pKa = 4.44 GVFSLVVVVDD134 pKa = 5.02 RR135 pKa = 11.84 KK136 pKa = 9.84 PHH138 pKa = 6.36 LGPTGCLHH146 pKa = 6.5 TFDD149 pKa = 4.87 EE150 pKa = 4.88 LFGARR155 pKa = 11.84 IHH157 pKa = 5.86 SHH159 pKa = 6.13 GNLSITPSLKK169 pKa = 9.27 DD170 pKa = 2.88 RR171 pKa = 11.84 FYY173 pKa = 10.59 IRR175 pKa = 11.84 HH176 pKa = 4.4 VFKK179 pKa = 10.81 RR180 pKa = 11.84 VLSVEE185 pKa = 4.19 KK186 pKa = 9.53 DD187 pKa = 3.15 TLMVDD192 pKa = 3.64 VEE194 pKa = 4.64 GSTTLSNRR202 pKa = 11.84 RR203 pKa = 11.84 YY204 pKa = 10.04 NLWSTFKK211 pKa = 11.17 DD212 pKa = 3.7 LDD214 pKa = 3.73 HH215 pKa = 7.44 DD216 pKa = 4.33 SCKK219 pKa = 10.65 GVYY222 pKa = 10.47 DD223 pKa = 4.52 NISKK227 pKa = 10.38 NALLVYY233 pKa = 8.04 YY234 pKa = 9.89 CWMSDD239 pKa = 3.31 TMSKK243 pKa = 11.05 ASTFVSFDD251 pKa = 3.33 LDD253 pKa = 4.08 YY254 pKa = 11.31 IGG256 pKa = 5.26
Molecular weight: 29.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.155
IPC2_protein 9.282
IPC_protein 9.297
Toseland 9.926
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.526
Grimsley 10.233
Solomon 10.175
Lehninger 10.145
Nozaki 9.955
DTASelect 9.823
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.072
Patrickios 9.897
IPC_peptide 10.175
IPC2_peptide 8.492
IPC2.peptide.svr19 8.234
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1507
85
361
215.3
24.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.844 ± 0.546
1.659 ± 0.23
5.176 ± 0.312
5.043 ± 0.537
4.247 ± 0.531
5.707 ± 0.561
3.716 ± 0.251
5.574 ± 0.547
6.238 ± 0.649
6.901 ± 0.579
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.322 ± 0.569
5.441 ± 0.583
5.574 ± 0.596
4.313 ± 0.882
6.901 ± 0.915
8.825 ± 1.272
6.038 ± 0.66
5.839 ± 0.755
1.593 ± 0.242
4.048 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here