Arthrobacter phage Oxynfrius
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9SE21|A0A1I9SE21_9CAUD HNH endonuclease OS=Arthrobacter phage Oxynfrius OX=1897429 GN=SEA_OXYNFRIUS_62 PE=4 SV=1
MM1 pKa = 7.8 DD2 pKa = 4.88 FCWEE6 pKa = 4.09 DD7 pKa = 3.71 SEE9 pKa = 5.03 TGCICGNEE17 pKa = 4.13 CEE19 pKa = 4.32 PPKK22 pKa = 10.63 WMIQMWEE29 pKa = 4.21 GEE31 pKa = 4.41 GG32 pKa = 3.51
Molecular weight: 3.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.008
IPC2_protein 3.783
IPC_protein 3.478
Toseland 3.376
ProMoST 3.694
Dawson 3.478
Bjellqvist 3.668
Wikipedia 3.427
Rodwell 3.363
Grimsley 3.312
Solomon 3.376
Lehninger 3.325
Nozaki 3.668
DTASelect 3.656
Thurlkill 3.465
EMBOSS 3.439
Sillero 3.617
Patrickios 0.006
IPC_peptide 3.376
IPC2_peptide 3.567
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|A0A1I9SE20|A0A1I9SE20_9CAUD Uncharacterized protein OS=Arthrobacter phage Oxynfrius OX=1897429 GN=SEA_OXYNFRIUS_61 PE=4 SV=1
MM1 pKa = 7.11 QATPEE6 pKa = 4.26 VKK8 pKa = 9.0 TRR10 pKa = 11.84 PVLPHH15 pKa = 5.65 TFGRR19 pKa = 11.84 PDD21 pKa = 3.18 MAGLTRR27 pKa = 11.84 MQRR30 pKa = 11.84 RR31 pKa = 11.84 GIAKK35 pKa = 10.16 LKK37 pKa = 8.71 RR38 pKa = 11.84 QKK40 pKa = 10.44 GQPSKK45 pKa = 11.23 VEE47 pKa = 3.75 RR48 pKa = 11.84 TQSKK52 pKa = 10.39 KK53 pKa = 10.1 RR54 pKa = 11.84 GKK56 pKa = 10.02
Molecular weight: 6.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 10.35
IPC_protein 11.842
Toseland 12.047
ProMoST 12.501
Dawson 12.047
Bjellqvist 12.018
Wikipedia 12.501
Rodwell 11.974
Grimsley 12.091
Solomon 12.515
Lehninger 12.427
Nozaki 12.032
DTASelect 12.018
Thurlkill 12.032
EMBOSS 12.53
Sillero 12.032
Patrickios 11.711
IPC_peptide 12.515
IPC2_peptide 11.491
IPC2.peptide.svr19 9.18
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
14024
32
876
226.2
24.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.218 ± 0.396
0.713 ± 0.152
5.647 ± 0.243
6.489 ± 0.331
2.988 ± 0.207
8.657 ± 0.352
1.875 ± 0.169
5.106 ± 0.307
5.056 ± 0.32
8.393 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.152
3.587 ± 0.181
5.141 ± 0.181
4.022 ± 0.168
5.59 ± 0.353
6.139 ± 0.343
6.482 ± 0.328
7.152 ± 0.324
1.989 ± 0.183
2.474 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here