Pythium polare bunya-like RNA virus 1
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6C4N5|A0A2Z6C4N5_9VIRU Uncharacterized protein OS=Pythium polare bunya-like RNA virus 1 OX=2137352 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.11 EE3 pKa = 5.63 DD4 pKa = 4.36 EE5 pKa = 5.61 LDD7 pKa = 3.57 EE8 pKa = 4.23 LTAFDD13 pKa = 5.44 MEE15 pKa = 4.47 VEE17 pKa = 4.14 DD18 pKa = 4.86 LNGEE22 pKa = 3.96 EE23 pKa = 4.96 RR24 pKa = 11.84 EE25 pKa = 4.42 WEE27 pKa = 4.14 GDD29 pKa = 3.53 DD30 pKa = 3.67 YY31 pKa = 11.87 KK32 pKa = 11.27 AVCWSYY38 pKa = 11.95 KK39 pKa = 10.12 DD40 pKa = 3.38 IKK42 pKa = 11.11 KK43 pKa = 10.54 LMQEE47 pKa = 3.82 TALNDD52 pKa = 3.67 FSSITICAPHH62 pKa = 7.22 ANLCFPWLGRR72 pKa = 11.84 GDD74 pKa = 3.66 YY75 pKa = 9.69 TYY77 pKa = 11.13 EE78 pKa = 4.52 KK79 pKa = 10.21 IGGVDD84 pKa = 3.19 MFVSRR89 pKa = 11.84 FPGGQHH95 pKa = 5.76 YY96 pKa = 10.54 NPPNFKK102 pKa = 9.37 EE103 pKa = 4.09 VKK105 pKa = 9.7 MGDD108 pKa = 3.48 EE109 pKa = 3.77 KK110 pKa = 10.52 TLRR113 pKa = 11.84 DD114 pKa = 3.65 RR115 pKa = 11.84 LKK117 pKa = 10.71 DD118 pKa = 3.22 IKK120 pKa = 10.43 PVNKK124 pKa = 9.91 EE125 pKa = 3.64 KK126 pKa = 10.49 IIPKK130 pKa = 8.65 VLKK133 pKa = 10.32 SRR135 pKa = 11.84 EE136 pKa = 3.93 EE137 pKa = 3.77 AVEE140 pKa = 3.77 VLKK143 pKa = 10.91 YY144 pKa = 11.03 LNIYY148 pKa = 8.81 NPKK151 pKa = 10.16 LVDD154 pKa = 3.22 ALYY157 pKa = 9.76 PRR159 pKa = 11.84 KK160 pKa = 10.12 KK161 pKa = 10.51 EE162 pKa = 4.18 DD163 pKa = 3.73 FLDD166 pKa = 3.56 MTSNMLSYY174 pKa = 10.87 FEE176 pKa = 5.1 KK177 pKa = 10.76 FVGNFDD183 pKa = 3.85 IQSHH187 pKa = 5.33 MIKK190 pKa = 10.31 RR191 pKa = 11.84 ANSKK195 pKa = 10.4 FNLPGFQGILDD206 pKa = 3.95 DD207 pKa = 4.73 SFLKK211 pKa = 11.08 AEE213 pKa = 4.42 LKK215 pKa = 10.92 SIFGEE220 pKa = 4.13 NFYY223 pKa = 11.2 HH224 pKa = 7.89 LLTGNFNLTRR234 pKa = 11.84 RR235 pKa = 11.84 SYY237 pKa = 11.94 DD238 pKa = 2.96 MYY240 pKa = 11.35 KK241 pKa = 11.19 AMILRR246 pKa = 11.84 NYY248 pKa = 8.59 NRR250 pKa = 11.84 CSNSDD255 pKa = 3.03 KK256 pKa = 10.95 SLSIFLLSLLQDD268 pKa = 3.59 CCITIGSDD276 pKa = 2.76 SWFLDD281 pKa = 2.98 VMNDD285 pKa = 3.14 VTFGIDD291 pKa = 3.01 EE292 pKa = 4.19 RR293 pKa = 11.84 ANPIFQEE300 pKa = 4.14 MIMPVLPQGMQIEE313 pKa = 4.52 YY314 pKa = 9.49 TEE316 pKa = 4.96 KK317 pKa = 11.32 DD318 pKa = 3.24 MFDD321 pKa = 3.21
Molecular weight: 37.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.779
IPC2_protein 4.8
IPC_protein 4.736
Toseland 4.584
ProMoST 4.813
Dawson 4.698
Bjellqvist 4.851
Wikipedia 4.571
Rodwell 4.584
Grimsley 4.495
Solomon 4.698
Lehninger 4.647
Nozaki 4.8
DTASelect 4.991
Thurlkill 4.596
EMBOSS 4.596
Sillero 4.863
Patrickios 3.961
IPC_peptide 4.698
IPC2_peptide 4.851
IPC2.peptide.svr19 4.839
Protein with the highest isoelectric point:
>tr|A0A2Z6C4N5|A0A2Z6C4N5_9VIRU Uncharacterized protein OS=Pythium polare bunya-like RNA virus 1 OX=2137352 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.3 SEE4 pKa = 4.51 RR5 pKa = 11.84 DD6 pKa = 3.33 LSEE9 pKa = 3.65 KK10 pKa = 10.49 SKK12 pKa = 9.87 WYY14 pKa = 10.28 SEE16 pKa = 4.46 EE17 pKa = 3.77 VSKK20 pKa = 11.11 SHH22 pKa = 5.86 FVSYY26 pKa = 10.26 VSWIMGLAQQSYY38 pKa = 9.84 KK39 pKa = 10.48 ISILDD44 pKa = 3.33 SKK46 pKa = 11.16 VYY48 pKa = 10.62 YY49 pKa = 10.37 KK50 pKa = 10.64 LRR52 pKa = 11.84 HH53 pKa = 5.79 NMLKK57 pKa = 9.69 TYY59 pKa = 10.29 ILNSFNLGNSPEE71 pKa = 4.24 KK72 pKa = 10.91 SFSFYY77 pKa = 11.35 NEE79 pKa = 4.14 LGLDD83 pKa = 3.67 SSRR86 pKa = 11.84 TPDD89 pKa = 3.51 YY90 pKa = 10.69 LIPAGDD96 pKa = 3.96 GFLLMEE102 pKa = 4.39 FTYY105 pKa = 10.58 SSKK108 pKa = 10.47 YY109 pKa = 9.43 QNVLKK114 pKa = 10.57 NKK116 pKa = 9.88 KK117 pKa = 9.46 VFSKK121 pKa = 10.33 YY122 pKa = 10.03 DD123 pKa = 3.37 YY124 pKa = 10.32 EE125 pKa = 4.12 IRR127 pKa = 11.84 EE128 pKa = 4.39 SKK130 pKa = 10.31 IPIYY134 pKa = 10.43 DD135 pKa = 3.47 YY136 pKa = 11.79 YY137 pKa = 11.68 LFLTLDD143 pKa = 4.31 DD144 pKa = 5.67 DD145 pKa = 4.34 IKK147 pKa = 11.5 DD148 pKa = 4.51 LISMMTHH155 pKa = 5.63 ISLRR159 pKa = 11.84 FGFEE163 pKa = 3.87 LEE165 pKa = 4.6 SNFEE169 pKa = 4.23 SEE171 pKa = 5.75 LEE173 pKa = 4.13 DD174 pKa = 3.0 MHH176 pKa = 6.32 NTVKK180 pKa = 10.6 EE181 pKa = 4.07 VTAYY185 pKa = 10.83 LNDD188 pKa = 4.36 YY189 pKa = 10.46 IPEE192 pKa = 4.61 LLTLNIDD199 pKa = 4.19 EE200 pKa = 5.04 IDD202 pKa = 3.15 IDD204 pKa = 4.41 FPVTSISVQEE214 pKa = 3.88 PFKK217 pKa = 10.8 IISNLRR223 pKa = 11.84 GVKK226 pKa = 9.71 SNKK229 pKa = 8.23 NVRR232 pKa = 11.84 IKK234 pKa = 11.07 SSITRR239 pKa = 11.84 NFRR242 pKa = 11.84 KK243 pKa = 9.29 LQRR246 pKa = 11.84 DD247 pKa = 3.6 IKK249 pKa = 10.41 RR250 pKa = 11.84 KK251 pKa = 9.97 HH252 pKa = 5.78 KK253 pKa = 10.31 DD254 pKa = 2.82 GRR256 pKa = 11.84 YY257 pKa = 9.0 RR258 pKa = 11.84 LCLNFTLQKK267 pKa = 10.53 VFIIEE272 pKa = 3.95 VSEE275 pKa = 4.0 GLKK278 pKa = 9.65 KK279 pKa = 10.11 HH280 pKa = 5.88 EE281 pKa = 4.24 MQSMLDD287 pKa = 3.65 NEE289 pKa = 5.29 DD290 pKa = 3.35 IAICEE295 pKa = 4.15 YY296 pKa = 10.5 TDD298 pKa = 3.42 IVGDD302 pKa = 3.88 SYY304 pKa = 12.02 SEE306 pKa = 3.98 DD307 pKa = 3.58 RR308 pKa = 11.84 LIKK311 pKa = 10.17 GLKK314 pKa = 8.93 NVYY317 pKa = 9.41 EE318 pKa = 4.28 PQDD321 pKa = 3.67 LEE323 pKa = 4.54 RR324 pKa = 11.84 EE325 pKa = 4.01 KK326 pKa = 11.34 CLITKK331 pKa = 9.9 IEE333 pKa = 4.13 SDD335 pKa = 3.72 TYY337 pKa = 10.77 LSRR340 pKa = 11.84 FNKK343 pKa = 9.7 YY344 pKa = 10.75 LNLSTMTTVADD355 pKa = 3.8 SNLDD359 pKa = 3.36 VDD361 pKa = 4.18 SLKK364 pKa = 11.12 VQDD367 pKa = 4.03 IYY369 pKa = 10.22 TAEE372 pKa = 4.04 MLKK375 pKa = 10.65 KK376 pKa = 10.24 NSGEE380 pKa = 3.93 NVVFYY385 pKa = 10.45 HH386 pKa = 6.66 KK387 pKa = 10.79 SPFIFYY393 pKa = 9.85 PCDD396 pKa = 3.42 YY397 pKa = 10.42 LVKK400 pKa = 10.67 GDD402 pKa = 4.17 FKK404 pKa = 11.65 VDD406 pKa = 3.12 VDD408 pKa = 4.37 SKK410 pKa = 11.26 SEE412 pKa = 3.65 IMNILIRR419 pKa = 11.84 HH420 pKa = 6.43 ALNKK424 pKa = 10.26 SVVSDD429 pKa = 3.42 RR430 pKa = 11.84 VIEE433 pKa = 3.93 RR434 pKa = 11.84 DD435 pKa = 3.03 IDD437 pKa = 3.54 YY438 pKa = 10.9 DD439 pKa = 4.07 SYY441 pKa = 11.77 VKK443 pKa = 10.42 YY444 pKa = 10.89 SRR446 pKa = 11.84 DD447 pKa = 3.34 VGVMGRR453 pKa = 11.84 NLRR456 pKa = 11.84 MKK458 pKa = 10.7 YY459 pKa = 8.85 GVEE462 pKa = 3.99 YY463 pKa = 10.66 KK464 pKa = 10.52 RR465 pKa = 11.84 LLNLRR470 pKa = 11.84 KK471 pKa = 9.24 RR472 pKa = 11.84 DD473 pKa = 4.37 FILEE477 pKa = 4.21 SEE479 pKa = 4.6 SKK481 pKa = 10.5 DD482 pKa = 3.48 LSDD485 pKa = 5.0 ISDD488 pKa = 4.01 FKK490 pKa = 11.31 NLRR493 pKa = 11.84 SKK495 pKa = 10.95 FSTIVKK501 pKa = 9.6 EE502 pKa = 4.46 STRR505 pKa = 11.84 TAYY508 pKa = 9.91 KK509 pKa = 10.56 NRR511 pKa = 11.84 IKK513 pKa = 10.74 INLKK517 pKa = 10.11 INNRR521 pKa = 11.84 WDD523 pKa = 4.68 DD524 pKa = 4.11 DD525 pKa = 3.69 MQHH528 pKa = 5.64 FQKK531 pKa = 10.76 LKK533 pKa = 8.83 NKK535 pKa = 8.8 TYY537 pKa = 10.36 IIGEE541 pKa = 4.03 YY542 pKa = 10.42 GYY544 pKa = 10.27 EE545 pKa = 3.97 KK546 pKa = 10.82 SKK548 pKa = 11.31 NEE550 pKa = 3.42 YY551 pKa = 9.91 RR552 pKa = 11.84 AFLSEE557 pKa = 4.37 LFEE560 pKa = 4.67 SSGTLASDD568 pKa = 5.46 DD569 pKa = 4.48 IYY571 pKa = 11.56 SDD573 pKa = 3.68 TLPLGGSLASLCGQMKK589 pKa = 10.77 SMIEE593 pKa = 4.08 PNVEE597 pKa = 4.17 LYY599 pKa = 10.67 RR600 pKa = 11.84 GTRR603 pKa = 11.84 LAHH606 pKa = 5.08 STLLISQVSHH616 pKa = 7.55 AIMYY620 pKa = 8.34 YY621 pKa = 10.96 SNIKK625 pKa = 9.71 LNKK628 pKa = 9.58 EE629 pKa = 3.56 DD630 pKa = 5.31 FIYY633 pKa = 11.11 DD634 pKa = 3.31 NLGYY638 pKa = 10.81 EE639 pKa = 4.13 NVLLVVKK646 pKa = 10.24 GGKK649 pKa = 9.32 KK650 pKa = 9.69 IKK652 pKa = 4.82 TTRR655 pKa = 11.84 YY656 pKa = 9.15 SRR658 pKa = 11.84 FFRR661 pKa = 11.84 LLLPITEE668 pKa = 4.39 CQSVLISSSSNKK680 pKa = 9.51 VLLHH684 pKa = 6.15 EE685 pKa = 4.73 GKK687 pKa = 10.42 KK688 pKa = 10.57 YY689 pKa = 10.98 LLTPWRR695 pKa = 11.84 NLRR698 pKa = 11.84 LTYY701 pKa = 10.13 LKK703 pKa = 10.62 KK704 pKa = 10.87 GFEE707 pKa = 4.45 TYY709 pKa = 10.82 HH710 pKa = 6.19 NFSNYY715 pKa = 9.77 YY716 pKa = 8.83 ISSFLEE722 pKa = 3.8 SDD724 pKa = 4.27 LNLEE728 pKa = 4.68 DD729 pKa = 4.08 FNKK732 pKa = 10.29 FISLKK737 pKa = 9.26 VLCLFSQKK745 pKa = 10.21 RR746 pKa = 11.84 KK747 pKa = 9.51 LEE749 pKa = 4.0 VWISTLRR756 pKa = 11.84 YY757 pKa = 9.09 IYY759 pKa = 10.81 LNSLSTHH766 pKa = 6.35 SDD768 pKa = 3.08 VLSLIKK774 pKa = 10.24 EE775 pKa = 4.09 MPIADD780 pKa = 4.61 TDD782 pKa = 3.78 TLIYY786 pKa = 10.2 FLQRR790 pKa = 11.84 SFAMSYY796 pKa = 10.32 KK797 pKa = 10.2 EE798 pKa = 3.72 IYY800 pKa = 8.69 HH801 pKa = 5.01 TVKK804 pKa = 10.4 EE805 pKa = 4.26 KK806 pKa = 10.85 KK807 pKa = 10.48 LYY809 pKa = 10.81 DD810 pKa = 4.08 LVWHH814 pKa = 5.59 NTISNFDD821 pKa = 3.48 LMAEE825 pKa = 3.98 RR826 pKa = 11.84 FEE828 pKa = 5.04 EE829 pKa = 4.35 NIFMSRR835 pKa = 11.84 APFNPYY841 pKa = 9.96 NEE843 pKa = 4.18 HH844 pKa = 6.4 LKK846 pKa = 10.39 NLKK849 pKa = 10.28 SVFDD853 pKa = 3.38 THH855 pKa = 7.46 KK856 pKa = 10.69 YY857 pKa = 9.84 YY858 pKa = 9.83 MDD860 pKa = 3.93 HH861 pKa = 6.54 VGSSDD866 pKa = 3.48 PMIGLEE872 pKa = 4.02 KK873 pKa = 8.85 TTVEE877 pKa = 3.56 LSEE880 pKa = 5.27 NYY882 pKa = 10.02 FSDD885 pKa = 5.59 LEE887 pKa = 4.46 TNDD890 pKa = 3.97 FNFDD894 pKa = 3.3 PKK896 pKa = 10.38 MSYY899 pKa = 10.71 LVGDD903 pKa = 4.04 YY904 pKa = 9.91 IGSFITSVKK913 pKa = 10.53 SEE915 pKa = 4.38 AEE917 pKa = 3.72 LHH919 pKa = 6.41 EE920 pKa = 5.04 DD921 pKa = 3.86 FNSILNRR928 pKa = 11.84 NFTEE932 pKa = 3.85 ISTSKK937 pKa = 10.72 GMRR940 pKa = 11.84 DD941 pKa = 3.07 SEE943 pKa = 4.73 GIFWGKK949 pKa = 9.97 KK950 pKa = 5.81 GHH952 pKa = 5.85 EE953 pKa = 4.33 VIFDD957 pKa = 3.61 KK958 pKa = 11.09 MNINIDD964 pKa = 3.54 EE965 pKa = 4.4 TLDD968 pKa = 3.56 NFPNDD973 pKa = 4.02 PIKK976 pKa = 10.58 FNKK979 pKa = 9.92 LISSFEE985 pKa = 3.75 KK986 pKa = 10.99 DD987 pKa = 3.91 FIDD990 pKa = 6.32 KK991 pKa = 10.47 IGEE994 pKa = 4.03 LNGIVLEE1001 pKa = 4.47 FDD1003 pKa = 4.44 LKK1005 pKa = 11.24 DD1006 pKa = 3.2 KK1007 pKa = 10.63 EE1008 pKa = 4.29 QYY1010 pKa = 9.71 KK1011 pKa = 10.22 GSRR1014 pKa = 11.84 EE1015 pKa = 3.96 IYY1017 pKa = 10.56 VMSDD1021 pKa = 2.76 KK1022 pKa = 11.05 TKK1024 pKa = 10.7 LLQNPLEE1031 pKa = 4.29 KK1032 pKa = 10.57 FFASLCKK1039 pKa = 10.24 CLPNEE1044 pKa = 5.2 LIHH1047 pKa = 6.62 KK1048 pKa = 8.55 PSSSRR1053 pKa = 11.84 PKK1055 pKa = 10.17 FIHH1058 pKa = 6.06 SKK1060 pKa = 9.75 IFEE1063 pKa = 4.43 FEE1065 pKa = 4.36 HH1066 pKa = 6.29 QDD1068 pKa = 3.48 QQIMYY1073 pKa = 8.62 CTMDD1077 pKa = 3.32 CRR1079 pKa = 11.84 KK1080 pKa = 8.62 WAPRR1084 pKa = 11.84 SNLWKK1089 pKa = 10.13 YY1090 pKa = 9.67 FYY1092 pKa = 10.22 FVKK1095 pKa = 10.71 GMAKK1099 pKa = 9.77 YY1100 pKa = 10.08 LPKK1103 pKa = 10.72 SFVDD1107 pKa = 3.35 YY1108 pKa = 10.63 FFKK1111 pKa = 10.76 FWTLMFKK1118 pKa = 10.41 KK1119 pKa = 10.36 RR1120 pKa = 11.84 IRR1122 pKa = 11.84 IQKK1125 pKa = 9.86 HH1126 pKa = 5.02 FIEE1129 pKa = 4.75 NLLKK1133 pKa = 10.69 NPSYY1137 pKa = 9.54 THH1139 pKa = 7.54 IEE1141 pKa = 4.03 DD1142 pKa = 3.89 YY1143 pKa = 11.05 LNKK1146 pKa = 10.34 RR1147 pKa = 11.84 EE1148 pKa = 4.68 DD1149 pKa = 3.77 GDD1151 pKa = 4.13 FEE1153 pKa = 5.82 LVMPYY1158 pKa = 10.58 SFMMGIFNYY1167 pKa = 10.24 LSSLMHH1173 pKa = 6.97 AASQLYY1179 pKa = 9.01 FSNVLMSDD1187 pKa = 3.68 YY1188 pKa = 10.64 KK1189 pKa = 11.28 VSLSFLAHH1197 pKa = 6.62 SDD1199 pKa = 3.46 DD1200 pKa = 3.65 SAGIIVSKK1208 pKa = 10.46 DD1209 pKa = 3.25 YY1210 pKa = 11.38 KK1211 pKa = 10.87 NCLKK1215 pKa = 10.49 FYY1217 pKa = 10.59 SFYY1220 pKa = 11.44 EE1221 pKa = 4.1 MYY1223 pKa = 10.25 QRR1225 pKa = 11.84 SLNHH1229 pKa = 5.32 LMSRR1233 pKa = 11.84 KK1234 pKa = 9.49 KK1235 pKa = 10.28 CALSKK1240 pKa = 10.71 RR1241 pKa = 11.84 SFEE1244 pKa = 5.27 LISIMYY1250 pKa = 8.85 CDD1252 pKa = 3.97 KK1253 pKa = 10.98 RR1254 pKa = 11.84 FIPMTHH1260 pKa = 6.56 KK1261 pKa = 10.32 FVSNIAFDD1269 pKa = 3.92 PKK1271 pKa = 10.57 GAGWYY1276 pKa = 10.12 NDD1278 pKa = 2.8 ITVVVGKK1285 pKa = 10.71 VVDD1288 pKa = 4.23 LFNNGGSLLQCYY1300 pKa = 10.51 CMMLSMSEE1308 pKa = 4.77 LIRR1311 pKa = 11.84 KK1312 pKa = 9.28 AYY1314 pKa = 9.58 HH1315 pKa = 6.24 LPRR1318 pKa = 11.84 SKK1320 pKa = 10.03 MLSHH1324 pKa = 7.05 IPLAFGGVFNMHH1336 pKa = 7.41 PLHH1339 pKa = 6.91 LILIGSSAQEE1349 pKa = 3.79 YY1350 pKa = 10.27 LLDD1353 pKa = 3.89 YY1354 pKa = 11.57 VEE1356 pKa = 6.13 DD1357 pKa = 4.85 DD1358 pKa = 3.5 DD1359 pKa = 6.38 SRR1361 pKa = 11.84 FKK1363 pKa = 10.9 RR1364 pKa = 11.84 IQQFCAVTGSYY1375 pKa = 10.7 SLNAGNNFKK1384 pKa = 11.37 YY1385 pKa = 9.69 MISYY1389 pKa = 9.86 VRR1391 pKa = 11.84 THH1393 pKa = 7.05 HH1394 pKa = 5.48 SQKK1397 pKa = 10.49 ILTEE1401 pKa = 4.09 EE1402 pKa = 4.07 QKK1404 pKa = 9.26 VTLSAVSTLPIKK1416 pKa = 9.77 STYY1419 pKa = 9.85 ISYY1422 pKa = 10.65 LKK1424 pKa = 10.32 HH1425 pKa = 6.05 HH1426 pKa = 6.88 NKK1428 pKa = 9.96 LFDD1431 pKa = 3.13 KK1432 pKa = 10.8 QYY1434 pKa = 9.9 IYY1436 pKa = 11.15 SLSGVDD1442 pKa = 3.6 TQQLLLATLYY1452 pKa = 9.5 FPCSVRR1458 pKa = 11.84 ISDD1461 pKa = 4.86 KK1462 pKa = 10.95 SIKK1465 pKa = 10.68 LKK1467 pKa = 10.79 DD1468 pKa = 3.72 LSSLYY1473 pKa = 10.56 LFSNMSEE1480 pKa = 3.86 NLIFDD1485 pKa = 3.49 QFSYY1489 pKa = 10.88 PRR1491 pKa = 11.84 GNFLSYY1497 pKa = 10.59 VKK1499 pKa = 10.24 QVEE1502 pKa = 4.38 NIKK1505 pKa = 10.57 FDD1507 pKa = 4.52 LNDD1510 pKa = 3.69 FNFVSLKK1517 pKa = 8.65 SCKK1520 pKa = 9.14 PVVYY1524 pKa = 8.95 NTLVNYY1530 pKa = 10.22 NYY1532 pKa = 10.46 NLSSNTLMTLSAIEE1546 pKa = 4.1 KK1547 pKa = 9.46 MPNLRR1552 pKa = 11.84 NIFYY1556 pKa = 10.73 DD1557 pKa = 3.44 PDD1559 pKa = 3.84 KK1560 pKa = 11.28 FDD1562 pKa = 3.41 VLKK1565 pKa = 10.84 DD1566 pKa = 3.52 YY1567 pKa = 10.21 MLNSLPGTLDD1577 pKa = 3.11 EE1578 pKa = 4.52 KK1579 pKa = 10.85 TEE1581 pKa = 4.1 FLKK1584 pKa = 11.31 SFDD1587 pKa = 3.72 PSEE1590 pKa = 4.36 KK1591 pKa = 9.24 VEE1593 pKa = 4.09 KK1594 pKa = 9.8 MRR1596 pKa = 11.84 SGYY1599 pKa = 10.93 LFMPSNVRR1607 pKa = 11.84 VDD1609 pKa = 3.37 TLSRR1613 pKa = 11.84 FFTYY1617 pKa = 11.12 SMLYY1621 pKa = 4.52 TTRR1624 pKa = 11.84 RR1625 pKa = 11.84 YY1626 pKa = 10.21 LISSKK1631 pKa = 10.2 RR1632 pKa = 11.84 PQLFVPDD1639 pKa = 4.17 DD1640 pKa = 3.92 VEE1642 pKa = 5.17 LEE1644 pKa = 4.41 SNNIDD1649 pKa = 3.89 YY1650 pKa = 10.89 LKK1652 pKa = 10.54 HH1653 pKa = 6.32 LCVCLKK1659 pKa = 10.93 VLDD1662 pKa = 4.3 NDD1664 pKa = 4.16 MKK1666 pKa = 11.22 HH1667 pKa = 6.73 KK1668 pKa = 10.87 DD1669 pKa = 3.34 IVLKK1673 pKa = 11.04 NLINCKK1679 pKa = 10.12 ACAKK1683 pKa = 10.41 FSGLNNHH1690 pKa = 6.49 LNDD1693 pKa = 3.82 YY1694 pKa = 10.88 LAMFSIRR1701 pKa = 11.84 DD1702 pKa = 3.72 NVSFKK1707 pKa = 10.92 PKK1709 pKa = 10.56 VPFLDD1714 pKa = 3.7 YY1715 pKa = 11.08 LSTQIRR1721 pKa = 11.84 GKK1723 pKa = 9.6 NVWFSGSDD1731 pKa = 3.88 FILYY1735 pKa = 9.16 TPFGSIEE1742 pKa = 3.71 NRR1744 pKa = 11.84 LINGEE1749 pKa = 4.31 THH1751 pKa = 4.89 TTWVLEE1757 pKa = 4.09 NPDD1760 pKa = 3.36 MLGYY1764 pKa = 10.5 LWEE1767 pKa = 5.22 LYY1769 pKa = 10.42 NISCITRR1776 pKa = 11.84 GINVDD1781 pKa = 3.02 KK1782 pKa = 10.33 PAYY1785 pKa = 9.27 QDD1787 pKa = 3.09 TGFSYY1792 pKa = 10.67 PRR1794 pKa = 11.84 LAFNDD1799 pKa = 3.64 FNNPYY1804 pKa = 10.41 LPQTYY1809 pKa = 8.92 TNSIVLSNSKK1819 pKa = 10.23 VLIRR1823 pKa = 11.84 DD1824 pKa = 3.27 YY1825 pKa = 11.26 TMPNIYY1831 pKa = 10.32 RR1832 pKa = 11.84 LGNKK1836 pKa = 9.25 FCLSDD1841 pKa = 3.27 RR1842 pKa = 11.84 TLDD1845 pKa = 3.67 FKK1847 pKa = 11.28 INSVYY1852 pKa = 10.62 DD1853 pKa = 3.39 INQNFYY1859 pKa = 10.67 EE1860 pKa = 4.33 SHH1862 pKa = 6.34 GLQDD1866 pKa = 3.08 IKK1868 pKa = 11.04 KK1869 pKa = 9.02 IIYY1872 pKa = 10.2 RR1873 pKa = 11.84 SDD1875 pKa = 3.7 LEE1877 pKa = 4.13 VDD1879 pKa = 3.11 KK1880 pKa = 10.78 NTLLRR1885 pKa = 11.84 YY1886 pKa = 9.65 FDD1888 pKa = 4.4 DD1889 pKa = 3.73 SKK1891 pKa = 11.26 LYY1893 pKa = 10.38 KK1894 pKa = 10.47 VVMNDD1899 pKa = 2.91 NSHH1902 pKa = 7.03 FSSNEE1907 pKa = 3.26 NKK1909 pKa = 10.08 YY1910 pKa = 10.72 VNNGLLGNTCSFTRR1924 pKa = 11.84 ALALADD1930 pKa = 3.44 EE1931 pKa = 4.87 AKK1933 pKa = 8.27 ITRR1936 pKa = 11.84 YY1937 pKa = 9.63 RR1938 pKa = 11.84 SSYY1941 pKa = 8.04 NQMYY1945 pKa = 8.78 IQRR1948 pKa = 11.84 GVLEE1952 pKa = 4.28 YY1953 pKa = 9.51 DD1954 pKa = 3.56 TVGGVPVLDD1963 pKa = 3.59 MFEE1966 pKa = 4.33 KK1967 pKa = 11.04 VNLARR1972 pKa = 11.84 LTQYY1976 pKa = 10.2 EE1977 pKa = 4.03 KK1978 pKa = 10.98 RR1979 pKa = 11.84 SFEE1982 pKa = 3.88 KK1983 pKa = 10.98 CLGDD1987 pKa = 3.65 EE1988 pKa = 4.25 EE1989 pKa = 6.12 LDD1991 pKa = 3.78 IRR1993 pKa = 11.84 DD1994 pKa = 3.72 EE1995 pKa = 4.36 RR1996 pKa = 11.84 NLIKK2000 pKa = 10.39 IRR2002 pKa = 11.84 DD2003 pKa = 3.96 KK2004 pKa = 11.46 LGLEE2008 pKa = 4.1 ALGTSIVLHH2017 pKa = 4.72 KK2018 pKa = 10.46 HH2019 pKa = 4.86 IFKK2022 pKa = 10.95 EE2023 pKa = 4.59 MIAGMLANLPIGILKK2038 pKa = 10.61 NIMNIMLKK2046 pKa = 10.42 CINDD2050 pKa = 3.68 CMLTYY2055 pKa = 10.66 PNVRR2059 pKa = 11.84 VDD2061 pKa = 3.38 TQFNGDD2067 pKa = 3.66 SKK2069 pKa = 11.1 SWWTIFISIYY2079 pKa = 9.92 NSKK2082 pKa = 10.77 DD2083 pKa = 3.03 MHH2085 pKa = 7.21 LLGIHH2090 pKa = 6.64 LASGLLRR2097 pKa = 11.84 CKK2099 pKa = 10.14 QDD2101 pKa = 3.33 NEE2103 pKa = 4.32 HH2104 pKa = 6.67 KK2105 pKa = 9.7 FWSIVSSDD2113 pKa = 3.33 VLMSVLSINKK2123 pKa = 8.82 RR2124 pKa = 11.84 HH2125 pKa = 5.71 FNNLISMCRR2134 pKa = 11.84 GLLVALSKK2142 pKa = 10.73 KK2143 pKa = 10.59 KK2144 pKa = 9.97 EE2145 pKa = 3.75 IHH2147 pKa = 6.88 DD2148 pKa = 3.38 ILYY2151 pKa = 10.52 EE2152 pKa = 4.14 GEE2154 pKa = 4.21 NVKK2157 pKa = 9.15 WRR2159 pKa = 11.84 PLFFAKK2165 pKa = 10.09 NQYY2168 pKa = 10.33 RR2169 pKa = 11.84 EE2170 pKa = 3.7 IDD2172 pKa = 3.73 LDD2174 pKa = 3.91 FDD2176 pKa = 4.14 MINNVRR2182 pKa = 11.84 NVKK2185 pKa = 10.08 GLALL2189 pKa = 3.93
Molecular weight: 256.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.208
IPC2_protein 7.658
IPC_protein 7.439
Toseland 7.249
ProMoST 8.053
Dawson 8.214
Bjellqvist 8.463
Wikipedia 8.17
Rodwell 8.273
Grimsley 7.351
Solomon 8.244
Lehninger 8.244
Nozaki 8.551
DTASelect 8.273
Thurlkill 8.331
EMBOSS 8.404
Sillero 8.595
Patrickios 4.126
IPC_peptide 8.229
IPC2_peptide 7.278
IPC2.peptide.svr19 7.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2510
321
2189
1255.0
146.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.55 ± 0.404
1.474 ± 0.182
7.052 ± 0.913
5.777 ± 0.926
5.976 ± 0.261
3.984 ± 0.604
2.191 ± 0.435
6.932 ± 0.323
8.606 ± 0.233
11.036 ± 0.635
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.386 ± 0.592
6.773 ± 0.25
2.829 ± 0.562
2.191 ± 0.139
4.303 ± 0.116
9.163 ± 1.494
4.024 ± 0.275
5.1 ± 0.34
0.876 ± 0.17
5.777 ± 0.652
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here