Streptococcus satellite phage Javan723

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW97|A0A4D5ZW97_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan723 OX=2558817 GN=JavanS723_0021 PE=4 SV=1
MM1 pKa = 7.5NEE3 pKa = 3.99LDD5 pKa = 3.58ISNTQAVFVSVVLIGILLYY24 pKa = 10.91LNHH27 pKa = 7.7RR28 pKa = 11.84DD29 pKa = 3.57RR30 pKa = 11.84KK31 pKa = 10.2KK32 pKa = 9.42SAQIEE37 pKa = 4.32RR38 pKa = 11.84EE39 pKa = 3.95NQQTIEE45 pKa = 4.26TPSDD49 pKa = 3.86DD50 pKa = 4.93LNPDD54 pKa = 3.26YY55 pKa = 11.29GRR57 pKa = 11.84YY58 pKa = 8.63IQLAGVNVWGEE69 pKa = 4.05MEE71 pKa = 3.9

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZUM4|A0A4D5ZUM4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan723 OX=2558817 GN=JavanS723_0020 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.36RR3 pKa = 11.84IATIFLLGLPKK14 pKa = 9.96VPKK17 pKa = 8.88MRR19 pKa = 11.84PIAYY23 pKa = 8.55PRR25 pKa = 11.84ATISNNPEE33 pKa = 3.14ARR35 pKa = 11.84GIYY38 pKa = 10.11LEE40 pKa = 4.06NKK42 pKa = 9.02KK43 pKa = 10.62

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

3451

43

507

132.7

15.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.39 ± 0.398

0.493 ± 0.138

5.448 ± 0.359

9.62 ± 0.746

4.173 ± 0.464

4.839 ± 0.408

1.304 ± 0.239

7.505 ± 0.445

9.447 ± 0.485

9.968 ± 0.472

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.281

5.419 ± 0.352

3.13 ± 0.401

4.057 ± 0.322

5.477 ± 0.215

5.332 ± 0.284

5.419 ± 0.502

5.651 ± 0.474

0.811 ± 0.16

4.202 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski