Paracoccus aestuariivivens
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4256 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L6J9Z4|A0A6L6J9Z4_9RHOB Glycosyltransferase OS=Paracoccus aestuariivivens OX=1820333 GN=GL286_13940 PE=4 SV=1
MM1 pKa = 8.23 RR2 pKa = 11.84 DD3 pKa = 3.23 TSVPSSDD10 pKa = 4.96 FMNDD14 pKa = 2.98 PQTDD18 pKa = 3.68 QSVSTSRR25 pKa = 11.84 AIRR28 pKa = 11.84 TEE30 pKa = 3.73 TSDD33 pKa = 3.46 ADD35 pKa = 3.7 SGISTRR41 pKa = 11.84 YY42 pKa = 7.35 TMNVGDD48 pKa = 4.01 TFVGNLATYY57 pKa = 10.75 SDD59 pKa = 4.32 NDD61 pKa = 3.62 WVRR64 pKa = 11.84 VTLTAGTYY72 pKa = 9.95 EE73 pKa = 4.01 IDD75 pKa = 3.61 LAGSGSSPVRR85 pKa = 11.84 DD86 pKa = 3.59 TYY88 pKa = 11.56 LRR90 pKa = 11.84 IYY92 pKa = 10.31 DD93 pKa = 3.62 ATGTLVTYY101 pKa = 10.86 NDD103 pKa = 4.47 DD104 pKa = 4.56 GGDD107 pKa = 3.66 GLNSHH112 pKa = 7.29 LSLTVTQPGTYY123 pKa = 8.9 YY124 pKa = 10.66 VSAGAFSSSYY134 pKa = 9.18 TGSYY138 pKa = 9.63 SLNVSRR144 pKa = 11.84 GAEE147 pKa = 3.73 PTVFTIAQIAAQLTDD162 pKa = 4.78 GYY164 pKa = 10.33 WDD166 pKa = 3.3 STDD169 pKa = 3.11 RR170 pKa = 11.84 GRR172 pKa = 11.84 RR173 pKa = 11.84 SFDD176 pKa = 3.16 VSPGEE181 pKa = 4.26 TINVDD186 pKa = 4.47 LSGLDD191 pKa = 3.17 ADD193 pKa = 4.29 GRR195 pKa = 11.84 RR196 pKa = 11.84 LAVAALAAWTDD207 pKa = 3.33 VSGIRR212 pKa = 11.84 FNSVNPYY219 pKa = 10.08 SGQTVHH225 pKa = 5.99 ITFGDD230 pKa = 3.94 DD231 pKa = 3.89 EE232 pKa = 5.21 SGAWSSSTTSGGEE245 pKa = 3.46 IWSSDD250 pKa = 3.62 VNVGRR255 pKa = 11.84 DD256 pKa = 2.94 WLDD259 pKa = 3.12 TYY261 pKa = 11.0 GTGFATYY268 pKa = 10.01 SYY270 pKa = 8.93 QTYY273 pKa = 9.13 IHH275 pKa = 7.16 EE276 pKa = 4.71 IGHH279 pKa = 6.42 ALGLGHH285 pKa = 7.58 AGNYY289 pKa = 9.08 NGSAVYY295 pKa = 8.45 GTDD298 pKa = 2.7 NTYY301 pKa = 10.85 INDD304 pKa = 3.2 SWQASVMSYY313 pKa = 10.02 FSQDD317 pKa = 2.89 DD318 pKa = 3.73 NTFVNASYY326 pKa = 10.46 AYY328 pKa = 10.68 VMSAMAADD336 pKa = 3.75 IAAIQEE342 pKa = 4.32 LYY344 pKa = 11.18 GRR346 pKa = 11.84 TTLRR350 pKa = 11.84 AGNTVYY356 pKa = 11.06 GEE358 pKa = 3.96 NSNAGGNYY366 pKa = 9.51 GRR368 pKa = 11.84 ISSILATGARR378 pKa = 11.84 DD379 pKa = 4.28 DD380 pKa = 3.58 IAFTIVDD387 pKa = 3.88 SQGVDD392 pKa = 3.32 TLNLSGDD399 pKa = 3.77 TANQRR404 pKa = 11.84 IIMTPGGISNAYY416 pKa = 9.39 GITGNIIITSGTVIEE431 pKa = 4.23 NLRR434 pKa = 11.84 AGSGNDD440 pKa = 3.45 YY441 pKa = 11.04 LSGNAAHH448 pKa = 5.85 NTIWGNAGNDD458 pKa = 3.7 MIIGGLGNDD467 pKa = 3.95 TLLGGAGRR475 pKa = 11.84 DD476 pKa = 3.65 TLNGGNGNDD485 pKa = 3.8 IYY487 pKa = 11.03 HH488 pKa = 7.48 ADD490 pKa = 3.54 GFDD493 pKa = 3.99 IISEE497 pKa = 4.18 GSNGGVDD504 pKa = 3.56 TVYY507 pKa = 11.17 SSGNFALTPNLEE519 pKa = 3.99 NLYY522 pKa = 9.16 LTGSAQTGTGNVAANTIVGNAQGNTLRR549 pKa = 11.84 GMGGNDD555 pKa = 3.42 ILNGAAGSDD564 pKa = 3.76 TMHH567 pKa = 6.91 GGAGNDD573 pKa = 3.51 TYY575 pKa = 11.08 HH576 pKa = 7.32 IDD578 pKa = 3.53 SQDD581 pKa = 3.45 RR582 pKa = 11.84 LVEE585 pKa = 3.98 LANEE589 pKa = 4.96 GIDD592 pKa = 3.65 TVVSAASFTLAANFEE607 pKa = 4.29 NLRR610 pKa = 11.84 LTGSAVFGTGNDD622 pKa = 3.49 AANTIVGNSLGNTIRR637 pKa = 11.84 GMAGNDD643 pKa = 3.07 ILNGGGGNDD652 pKa = 3.1 RR653 pKa = 11.84 LFGGTHH659 pKa = 6.95 ADD661 pKa = 3.34 TFVFGSGQDD670 pKa = 3.26 MVMDD674 pKa = 4.08 FQNNIDD680 pKa = 3.84 TLRR683 pKa = 11.84 IDD685 pKa = 3.54 DD686 pKa = 4.63 ALWGGGARR694 pKa = 11.84 TVGQVLSLATVINGDD709 pKa = 3.85 TVFLFGNGNRR719 pKa = 11.84 LTIDD723 pKa = 2.97 NYY725 pKa = 10.85 TNINSLSDD733 pKa = 3.65 DD734 pKa = 3.84 LVIII738 pKa = 4.8
Molecular weight: 77.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.91
IPC_protein 3.961
Toseland 3.719
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.783
Grimsley 3.63
Solomon 3.973
Lehninger 3.935
Nozaki 4.075
DTASelect 4.393
Thurlkill 3.783
EMBOSS 3.948
Sillero 4.088
Patrickios 1.545
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.958
Protein with the highest isoelectric point:
>tr|A0A6L6J370|A0A6L6J370_9RHOB Uncharacterized protein OS=Paracoccus aestuariivivens OX=1820333 GN=GL286_02455 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.0 GRR40 pKa = 11.84 KK41 pKa = 8.91 SLSAA45 pKa = 3.86
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4256
0
4256
1347648
29
2777
316.6
34.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.279 ± 0.04
0.815 ± 0.012
5.81 ± 0.032
5.605 ± 0.036
3.63 ± 0.025
8.667 ± 0.038
2.067 ± 0.019
5.494 ± 0.028
2.991 ± 0.03
10.331 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.786 ± 0.017
2.624 ± 0.02
5.229 ± 0.027
3.304 ± 0.021
6.992 ± 0.043
5.428 ± 0.027
5.282 ± 0.024
7.097 ± 0.025
1.431 ± 0.015
2.138 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here