Penicillium brevicompactum tetramycovirus 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus; Penicillum brevicompactum polymycovirus 1

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3C4P0|A0A3G3C4P0_9VIRU RNA-dependent RNA polymerase OS=Penicillium brevicompactum tetramycovirus 1 OX=2485923 PE=4 SV=1
MM1 pKa = 8.02ADD3 pKa = 3.73LTRR6 pKa = 11.84LASIALNVDD15 pKa = 3.43SPAATLAAVVYY26 pKa = 7.15YY27 pKa = 9.31TGKK30 pKa = 8.02VTPGAEE36 pKa = 4.11YY37 pKa = 10.93VEE39 pKa = 4.41PAVLRR44 pKa = 11.84DD45 pKa = 3.55VLGWIAASPMLSIGNVVNEE64 pKa = 3.93EE65 pKa = 3.45AVAYY69 pKa = 9.77VRR71 pKa = 11.84LYY73 pKa = 10.94NGSDD77 pKa = 3.08KK78 pKa = 11.37LNNSVLDD85 pKa = 3.91DD86 pKa = 3.64ASYY89 pKa = 10.57YY90 pKa = 10.37LRR92 pKa = 11.84HH93 pKa = 6.42FGPLGGSYY101 pKa = 9.91TKK103 pKa = 10.34DD104 pKa = 2.84ARR106 pKa = 11.84TVRR109 pKa = 11.84VAEE112 pKa = 4.71IINHH116 pKa = 5.91ASIHH120 pKa = 5.39QGEE123 pKa = 4.72ATTGPVEE130 pKa = 4.13TALLEE135 pKa = 4.44SVSVAGYY142 pKa = 9.88EE143 pKa = 4.25GLAGALGAVSRR154 pKa = 11.84LHH156 pKa = 5.78LQQVIDD162 pKa = 3.96KK163 pKa = 9.13PDD165 pKa = 3.39VVVTQLDD172 pKa = 3.63RR173 pKa = 11.84AVFTASTVARR183 pKa = 11.84EE184 pKa = 4.04ATGEE188 pKa = 4.03HH189 pKa = 6.7RR190 pKa = 11.84FLATPPCVSRR200 pKa = 11.84QSAIACLAVMLSVTHH215 pKa = 6.56RR216 pKa = 11.84RR217 pKa = 11.84LVTDD221 pKa = 4.97RR222 pKa = 11.84IRR224 pKa = 11.84EE225 pKa = 3.97KK226 pKa = 10.32ATTVTSMAARR236 pKa = 11.84IDD238 pKa = 3.78LEE240 pKa = 4.02AVRR243 pKa = 11.84DD244 pKa = 3.65IVYY247 pKa = 8.56PARR250 pKa = 11.84EE251 pKa = 4.65LITNYY256 pKa = 9.75IFDD259 pKa = 4.29PAEE262 pKa = 3.86LVLRR266 pKa = 11.84DD267 pKa = 3.19QNGRR271 pKa = 11.84VRR273 pKa = 11.84EE274 pKa = 4.12RR275 pKa = 11.84ATSRR279 pKa = 11.84SPAAIVAFAFLTSRR293 pKa = 11.84GSSDD297 pKa = 3.82VLMDD301 pKa = 4.13LANRR305 pKa = 11.84RR306 pKa = 11.84VDD308 pKa = 3.55AVVDD312 pKa = 3.68KK313 pKa = 11.13DD314 pKa = 3.71SLVPHH319 pKa = 5.21VQEE322 pKa = 4.85YY323 pKa = 10.15YY324 pKa = 10.75DD325 pKa = 3.63NSRR328 pKa = 11.84TIYY331 pKa = 10.66SSLLLFLRR339 pKa = 11.84ALEE342 pKa = 4.05GTRR345 pKa = 11.84WEE347 pKa = 4.12RR348 pKa = 11.84ANNGRR353 pKa = 11.84GEE355 pKa = 4.31DD356 pKa = 3.97VNKK359 pKa = 10.34GPCLGYY365 pKa = 9.18MAAGRR370 pKa = 11.84TRR372 pKa = 11.84RR373 pKa = 11.84DD374 pKa = 3.19RR375 pKa = 11.84NTAAAVNRR383 pKa = 11.84LSEE386 pKa = 4.21MVVEE390 pKa = 4.16NRR392 pKa = 11.84EE393 pKa = 4.16LVPEE397 pKa = 4.81AGRR400 pKa = 11.84ILYY403 pKa = 9.92ILDD406 pKa = 3.18WNGVYY411 pKa = 10.26DD412 pKa = 4.09RR413 pKa = 11.84SVVLGAASLMKK424 pKa = 9.85IDD426 pKa = 3.79VALDD430 pKa = 3.35MGASGHH436 pKa = 6.67DD437 pKa = 3.5VGGVANPDD445 pKa = 3.41KK446 pKa = 11.0ASQTRR451 pKa = 11.84HH452 pKa = 4.22YY453 pKa = 9.63TVLLPALPGRR463 pKa = 11.84SMPTCSTVEE472 pKa = 3.97YY473 pKa = 10.7QPGDD477 pKa = 3.33GTASRR482 pKa = 11.84IRR484 pKa = 11.84ILVDD488 pKa = 4.51AIGGFNDD495 pKa = 3.87AYY497 pKa = 10.93SYY499 pKa = 10.88IYY501 pKa = 10.71GGTPADD507 pKa = 3.72KK508 pKa = 10.15TITPAVSVDD517 pKa = 3.22EE518 pKa = 4.38AQARR522 pKa = 11.84VFAVSGSSVGIPNALSTVDD541 pKa = 3.49ILLPPSCYY549 pKa = 9.53HH550 pKa = 6.43WPATPVDD557 pKa = 3.8TMVDD561 pKa = 3.25TFGDD565 pKa = 3.47VDD567 pKa = 4.41EE568 pKa = 5.81SCMSCQDD575 pKa = 2.97SVRR578 pKa = 11.84ALRR581 pKa = 11.84RR582 pKa = 11.84TAEE585 pKa = 4.14LLSVVGARR593 pKa = 11.84LVKK596 pKa = 8.74TRR598 pKa = 11.84SAFGHH603 pKa = 5.92NAQFDD608 pKa = 4.28IEE610 pKa = 4.45VSPAIRR616 pKa = 11.84DD617 pKa = 3.75GASDD621 pKa = 3.42TGVTLDD627 pKa = 3.34ATAMLNHH634 pKa = 6.17MRR636 pKa = 11.84NKK638 pKa = 10.43DD639 pKa = 3.14WGGDD643 pKa = 3.4TFRR646 pKa = 11.84PGGVVASTNQYY657 pKa = 7.6TAEE660 pKa = 3.9VDD662 pKa = 4.18EE663 pKa = 4.79EE664 pKa = 4.33CHH666 pKa = 7.27AILTHH671 pKa = 7.06IRR673 pKa = 11.84MLTYY677 pKa = 10.63EE678 pKa = 4.44DD679 pKa = 3.66LAGIGEE685 pKa = 4.47AAGPLPSSDD694 pKa = 3.61FDD696 pKa = 3.97DD697 pKa = 3.92VARR700 pKa = 11.84SLGG703 pKa = 3.51

Molecular weight: 75.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3C4W9|A0A3G3C4W9_9VIRU Methyltransferase OS=Penicillium brevicompactum tetramycovirus 1 OX=2485923 PE=4 SV=1
MM1 pKa = 7.43SSHH4 pKa = 6.18VGKK7 pKa = 10.23IASFLSTADD16 pKa = 3.49LSRR19 pKa = 11.84VRR21 pKa = 11.84TLNNEE26 pKa = 3.77DD27 pKa = 3.1VQLVVRR33 pKa = 11.84CLAAGVPPKK42 pKa = 10.83AFAEE46 pKa = 4.41TLGSYY51 pKa = 10.37DD52 pKa = 3.45PNKK55 pKa = 10.43EE56 pKa = 3.8YY57 pKa = 11.02DD58 pKa = 3.9VLPSAGPVPTRR69 pKa = 11.84KK70 pKa = 9.31ILAYY74 pKa = 10.36SFLRR78 pKa = 11.84DD79 pKa = 3.06RR80 pKa = 11.84RR81 pKa = 11.84QYY83 pKa = 11.6ADD85 pKa = 3.55TYY87 pKa = 10.06AQSDD91 pKa = 3.5EE92 pKa = 4.0WAGRR96 pKa = 11.84LRR98 pKa = 11.84GLLDD102 pKa = 3.81TDD104 pKa = 3.55PKK106 pKa = 10.71KK107 pKa = 10.85AVDD110 pKa = 4.35EE111 pKa = 4.39ISTVVNRR118 pKa = 11.84RR119 pKa = 11.84LRR121 pKa = 11.84VKK123 pKa = 10.57GSPAVADD130 pKa = 3.67VEE132 pKa = 4.65VGQVRR137 pKa = 11.84GSTGSEE143 pKa = 3.76ATGGASDD150 pKa = 4.46PNYY153 pKa = 11.1APLQQAMLKK162 pKa = 10.36YY163 pKa = 9.81SAQAGAFKK171 pKa = 10.39FDD173 pKa = 4.09AADD176 pKa = 3.69CGTPRR181 pKa = 11.84DD182 pKa = 3.55RR183 pKa = 11.84RR184 pKa = 11.84FVVTLGARR192 pKa = 11.84LIVGAQKK199 pKa = 10.81KK200 pKa = 7.98STAIMAARR208 pKa = 11.84LCRR211 pKa = 11.84VLGRR215 pKa = 11.84HH216 pKa = 6.12DD217 pKa = 4.2RR218 pKa = 11.84VVSPYY223 pKa = 10.0VGHH226 pKa = 6.61VDD228 pKa = 3.78EE229 pKa = 5.74EE230 pKa = 4.66EE231 pKa = 3.89DD232 pKa = 4.17HH233 pKa = 6.96EE234 pKa = 4.38WDD236 pKa = 3.55EE237 pKa = 4.98EE238 pKa = 4.34IPADD242 pKa = 3.85VADD245 pKa = 4.77GKK247 pKa = 9.46PVRR250 pKa = 11.84TADD253 pKa = 3.82RR254 pKa = 11.84AKK256 pKa = 10.89SPTTTRR262 pKa = 11.84SATPTSPKK270 pKa = 10.43GGAAGAAGGKK280 pKa = 9.37

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2370

280

775

592.5

64.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.549 ± 0.827

1.308 ± 0.098

7.046 ± 0.173

4.937 ± 0.338

3.333 ± 0.557

7.553 ± 0.262

2.363 ± 0.224

3.882 ± 0.278

3.502 ± 0.597

8.354 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.39

2.616 ± 0.308

5.485 ± 0.547

2.616 ± 0.38

7.764 ± 0.234

7.384 ± 0.306

5.738 ± 0.436

9.705 ± 0.659

0.844 ± 0.056

2.7 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski