candidate division MSBL1 archaeon SCGC-AAA259B11

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133U2V8|A0A133U2V8_9EURY N-acetyltransferase domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA259B11 OX=1698260 GN=AKJ61_04735 PE=4 SV=1
MM1 pKa = 7.05ITLDD5 pKa = 4.05KK6 pKa = 10.34KK7 pKa = 10.64AQGSTEE13 pKa = 3.87YY14 pKa = 10.29MLILAVVLVVAAGAIYY30 pKa = 9.71FITRR34 pKa = 11.84SGGNYY39 pKa = 8.29PAVSATPVAKK49 pKa = 10.3DD50 pKa = 3.56ANNDD54 pKa = 3.29GSDD57 pKa = 3.61DD58 pKa = 4.01LVILVEE64 pKa = 4.44ANSIPSGEE72 pKa = 4.02WKK74 pKa = 10.27VVYY77 pKa = 10.58NGTTVSPDD85 pKa = 3.21EE86 pKa = 4.67PLEE89 pKa = 3.98QPQYY93 pKa = 10.94IVDD96 pKa = 3.92DD97 pKa = 5.13NIPEE101 pKa = 4.02DD102 pKa = 3.93TYY104 pKa = 11.69EE105 pKa = 4.12VTLSHH110 pKa = 6.73IPSGHH115 pKa = 5.68VYY117 pKa = 9.3FNEE120 pKa = 5.08RR121 pKa = 11.84ITIQQ125 pKa = 3.06

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133U5K0|A0A133U5K0_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259B11 OX=1698260 GN=AKJ61_02925 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84NKK5 pKa = 9.44TLSTKK10 pKa = 10.5RR11 pKa = 11.84KK12 pKa = 8.02LAKK15 pKa = 9.97AKK17 pKa = 9.62KK18 pKa = 7.82QNRR21 pKa = 11.84RR22 pKa = 11.84VPIWVIAKK30 pKa = 8.71TGGRR34 pKa = 11.84VRR36 pKa = 11.84NNPKK40 pKa = 8.77RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.56WRR45 pKa = 11.84RR46 pKa = 11.84QNIKK50 pKa = 9.78PP51 pKa = 3.58

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

852

0

852

192211

38

1234

225.6

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.109 ± 0.09

1.052 ± 0.042

5.742 ± 0.079

10.265 ± 0.126

3.886 ± 0.062

7.564 ± 0.096

1.623 ± 0.039

7.068 ± 0.087

7.503 ± 0.101

8.96 ± 0.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.04

3.824 ± 0.059

4.202 ± 0.057

2.229 ± 0.04

5.687 ± 0.081

6.316 ± 0.082

4.788 ± 0.066

6.893 ± 0.067

1.127 ± 0.038

2.959 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski