Halioglobus sp. HI00S01

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Halioglobus; unclassified Halioglobus

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162H7Y7|A0A162H7Y7_9GAMM Uncharacterized protein OS=Halioglobus sp. HI00S01 OX=1822214 GN=A3709_00840 PE=4 SV=1
MM1 pKa = 6.86ITRR4 pKa = 11.84FASLSSRR11 pKa = 11.84LAATLIALCAITACGGGGGGGGGGGFLGDD40 pKa = 4.02SGGSSDD46 pKa = 3.81TYY48 pKa = 10.62FIQVALKK55 pKa = 10.69DD56 pKa = 3.53SDD58 pKa = 4.03GNEE61 pKa = 4.19TSTVTPNSPGTLQVLVTQNNPNGNPIADD89 pKa = 4.14VIVTASTNSGLLFPSSGSKK108 pKa = 8.48LTNAEE113 pKa = 3.75GAVTFRR119 pKa = 11.84IEE121 pKa = 4.04AGNEE125 pKa = 3.62SRR127 pKa = 11.84GAGTIEE133 pKa = 4.19VSVEE137 pKa = 3.9DD138 pKa = 3.73PSGNVVTEE146 pKa = 4.36SINFQLGVNGLRR158 pKa = 11.84LGFILDD164 pKa = 3.58GVYY167 pKa = 10.53YY168 pKa = 10.47DD169 pKa = 4.49AEE171 pKa = 4.37IGIAPNEE178 pKa = 4.39PIAAQGSAILALAVVDD194 pKa = 4.41EE195 pKa = 4.64NDD197 pKa = 3.44VPVAGAEE204 pKa = 4.33SVRR207 pKa = 11.84ITSACINSGEE217 pKa = 4.38STLSPEE223 pKa = 4.38NPIAVPNGQITVDD236 pKa = 3.88YY237 pKa = 10.81QVGSCSGIDD246 pKa = 3.3QLTAEE251 pKa = 4.92IIGTEE256 pKa = 3.84AQAFGTIEE264 pKa = 3.96IAEE267 pKa = 4.28PAADD271 pKa = 3.44SLTFISADD279 pKa = 3.13PTLIVLRR286 pKa = 11.84GTGGGPDD293 pKa = 2.9RR294 pKa = 11.84KK295 pKa = 9.66EE296 pKa = 3.56QSEE299 pKa = 4.79VKK301 pKa = 10.51FEE303 pKa = 4.31AVDD306 pKa = 3.66SQGNPQEE313 pKa = 4.07GVMVDD318 pKa = 4.46FSLTTDD324 pKa = 3.01VGGITFNPASATTDD338 pKa = 2.99AEE340 pKa = 4.97GIVSTFVNSGDD351 pKa = 3.32VATVVRR357 pKa = 11.84VVATMDD363 pKa = 3.14AMNGEE368 pKa = 4.57GTVSAVSDD376 pKa = 3.72VLTISTGLPDD386 pKa = 3.58QNSISLSVEE395 pKa = 3.81GTSVMIEE402 pKa = 3.63DD403 pKa = 4.09AMRR406 pKa = 11.84VDD408 pKa = 5.08GITRR412 pKa = 11.84AITVRR417 pKa = 11.84MADD420 pKa = 3.31KK421 pKa = 10.68FNNPVPDD428 pKa = 3.34GTAAVFTTEE437 pKa = 3.94YY438 pKa = 11.14GSIEE442 pKa = 4.41SACQTTNGACSVTWTSQAPRR462 pKa = 11.84FPTLPEE468 pKa = 3.78NQEE471 pKa = 4.21LVRR474 pKa = 11.84TIYY477 pKa = 10.81DD478 pKa = 3.18ADD480 pKa = 4.04YY481 pKa = 11.25ACPSHH486 pKa = 6.25NANGGPCPDD495 pKa = 3.94DD496 pKa = 4.01LGFIRR501 pKa = 11.84GARR504 pKa = 11.84STILVTAIGEE514 pKa = 4.15EE515 pKa = 4.25SFIDD519 pKa = 3.59ANANGIYY526 pKa = 10.27DD527 pKa = 3.61EE528 pKa = 4.78GEE530 pKa = 3.73RR531 pKa = 11.84FANLTEE537 pKa = 4.7AFLDD541 pKa = 3.99NNWNGFYY548 pKa = 10.9DD549 pKa = 3.84PARR552 pKa = 11.84TYY554 pKa = 10.81CVNNPTLRR562 pKa = 11.84EE563 pKa = 3.9CRR565 pKa = 11.84AGSEE569 pKa = 4.61EE570 pKa = 4.94IFTDD574 pKa = 4.36FNNNGVFDD582 pKa = 5.21ANGDD586 pKa = 3.89DD587 pKa = 4.23PANGYY592 pKa = 9.15PDD594 pKa = 3.69EE595 pKa = 4.88GVTAVYY601 pKa = 10.6NGLLCPPEE609 pKa = 5.79GDD611 pKa = 3.2GVYY614 pKa = 10.44CSRR617 pKa = 11.84EE618 pKa = 4.03LVNVRR623 pKa = 11.84DD624 pKa = 4.26DD625 pKa = 3.83LQIILSDD632 pKa = 3.64NPAWDD637 pKa = 3.48VLVVDD642 pKa = 4.06NRR644 pKa = 11.84GNAVTDD650 pKa = 3.38TAFNGGEE657 pKa = 4.05YY658 pKa = 9.73QVYY661 pKa = 10.23VSDD664 pKa = 4.17TFNNAPIAGTTISVTSSVCEE684 pKa = 3.98VNLSGNTEE692 pKa = 3.99VPNAILPGAFGVSLTQGGSVTYY714 pKa = 10.26ASCRR718 pKa = 11.84DD719 pKa = 3.39EE720 pKa = 4.42EE721 pKa = 4.55RR722 pKa = 11.84PSNTSEE728 pKa = 3.7FQINVGSEE736 pKa = 3.96YY737 pKa = 11.03NEE739 pKa = 4.12TFSCVIQVIDD749 pKa = 4.75DD750 pKa = 4.32ASDD753 pKa = 3.61CPEE756 pKa = 4.35PEE758 pKa = 4.57GGGGGDD764 pKa = 4.45DD765 pKa = 3.76GLSNGGG771 pKa = 3.49

Molecular weight: 79.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165UE41|A0A165UE41_9GAMM Dehydratase OS=Halioglobus sp. HI00S01 OX=1822214 GN=A3709_14885 PE=4 SV=1
MM1 pKa = 7.3VLTACLLVSKK11 pKa = 9.88VNRR14 pKa = 11.84ATRR17 pKa = 11.84AHH19 pKa = 5.59QVQPEE24 pKa = 4.13QQVQPEE30 pKa = 3.93QRR32 pKa = 11.84EE33 pKa = 3.9QRR35 pKa = 11.84EE36 pKa = 4.19QQVLLVPKK44 pKa = 9.33EE45 pKa = 3.95KK46 pKa = 10.39RR47 pKa = 11.84ATRR50 pKa = 11.84VPPAPLAHH58 pKa = 6.43APYY61 pKa = 8.84RR62 pKa = 11.84AKK64 pKa = 10.64RR65 pKa = 11.84PFHH68 pKa = 6.47VVTHH72 pKa = 6.52RR73 pKa = 11.84CQYY76 pKa = 10.08PVFVLILQPFSS87 pKa = 3.34

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4067

0

4067

1321789

38

3378

325.0

35.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.661 ± 0.046

1.031 ± 0.014

6.166 ± 0.028

6.593 ± 0.035

3.676 ± 0.023

7.933 ± 0.038

2.185 ± 0.02

5.099 ± 0.028

3.212 ± 0.028

10.284 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.018

3.257 ± 0.021

4.635 ± 0.024

3.958 ± 0.022

6.071 ± 0.029

6.028 ± 0.03

5.188 ± 0.023

7.173 ± 0.03

1.393 ± 0.016

2.873 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski