Planococcus sp. Y42
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3921 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q2L493|A0A1Q2L493_9BACL Uncharacterized protein OS=Planococcus sp. Y42 OX=2213202 GN=B0X71_18840 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.62 KK3 pKa = 10.49 KK4 pKa = 10.17 MNLFLLLSTAIMMVGCTDD22 pKa = 3.5 AEE24 pKa = 4.21 EE25 pKa = 4.3 SAVAGEE31 pKa = 4.27 EE32 pKa = 4.27 TVSQEE37 pKa = 3.74 VAEE40 pKa = 4.81 AEE42 pKa = 4.09 TEE44 pKa = 4.37 EE45 pKa = 4.48 ISEE48 pKa = 4.15 QAEE51 pKa = 4.0 TDD53 pKa = 3.48 TAVADD58 pKa = 3.94 EE59 pKa = 4.93 TEE61 pKa = 4.6 TEE63 pKa = 3.95 ASPEE67 pKa = 4.0 EE68 pKa = 4.29 SSEE71 pKa = 4.36 AEE73 pKa = 3.85 AAPSDD78 pKa = 4.39 VEE80 pKa = 4.32 SAPAQPEE87 pKa = 3.91 EE88 pKa = 4.35 APFPGYY94 pKa = 10.39 EE95 pKa = 4.68 LITVDD100 pKa = 5.04 GGDD103 pKa = 3.07 LSGYY107 pKa = 9.69 RR108 pKa = 11.84 EE109 pKa = 4.27 PNVVVDD115 pKa = 3.08 VGYY118 pKa = 10.95 GEE120 pKa = 4.04 RR121 pKa = 11.84 EE122 pKa = 3.6 YY123 pKa = 10.44 WAFTNEE129 pKa = 3.58 YY130 pKa = 8.34 GQLVRR135 pKa = 11.84 VVADD139 pKa = 4.42 EE140 pKa = 6.08 IIPQDD145 pKa = 4.07 DD146 pKa = 3.47 NSEE149 pKa = 4.07 PVTADD154 pKa = 2.51 GRR156 pKa = 11.84 YY157 pKa = 9.03 YY158 pKa = 9.98 PDD160 pKa = 3.36 EE161 pKa = 4.51 AKK163 pKa = 11.02 VPGVEE168 pKa = 4.89 SDD170 pKa = 3.86 VLDD173 pKa = 4.46 EE174 pKa = 4.33 GHH176 pKa = 7.0 VIADD180 pKa = 3.72 SLGGVANAYY189 pKa = 10.48 NITPQDD195 pKa = 3.29 STLNRR200 pKa = 11.84 HH201 pKa = 5.53 GDD203 pKa = 3.34 QAYY206 pKa = 8.73 MEE208 pKa = 4.63 DD209 pKa = 4.09 VIRR212 pKa = 11.84 DD213 pKa = 3.58 AGGATHH219 pKa = 7.12 FEE221 pKa = 4.53 AIITYY226 pKa = 9.59 PNTEE230 pKa = 3.85 TQIPSRR236 pKa = 11.84 YY237 pKa = 8.33 QYY239 pKa = 10.59 TYY241 pKa = 9.06 TVMGNEE247 pKa = 4.51 VVDD250 pKa = 3.48 TFNNVNPDD258 pKa = 3.54 EE259 pKa = 4.54 VNEE262 pKa = 4.18 SLGLTEE268 pKa = 5.35 NEE270 pKa = 4.05 PAEE273 pKa = 4.35 PADD276 pKa = 3.58 TDD278 pKa = 3.78 TAHH281 pKa = 7.23 DD282 pKa = 4.03 VSSVDD287 pKa = 3.51 TNGNGQVTIKK297 pKa = 9.56 EE298 pKa = 3.92 AKK300 pKa = 9.66 AAGFSMPIMSDD311 pKa = 2.56 HH312 pKa = 6.56 WLYY315 pKa = 10.53 PYY317 pKa = 9.86 MQDD320 pKa = 3.21 NDD322 pKa = 3.64 KK323 pKa = 11.57 DD324 pKa = 4.13 GMVGEE329 pKa = 4.54
Molecular weight: 35.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.516
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.579
Rodwell 3.541
Grimsley 3.427
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.834
Patrickios 1.265
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A1Q2KVC1|A0A1Q2KVC1_9BACL Uncharacterized protein OS=Planococcus sp. Y42 OX=2213202 GN=B0X71_02645 PE=4 SV=1
MM1 pKa = 7.34 FFHH4 pKa = 6.8 NRR6 pKa = 11.84 TASAAKK12 pKa = 9.62 ARR14 pKa = 11.84 RR15 pKa = 11.84 IKK17 pKa = 9.97 TSAPAPLRR25 pKa = 11.84 LLVANSSGKK34 pKa = 10.16 AVAAAVRR41 pKa = 11.84 WLPPFHH47 pKa = 7.1 SRR49 pKa = 11.84 QLKK52 pKa = 6.71 MTTRR56 pKa = 11.84 QNAA59 pKa = 3.02
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.501
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.237
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3921
0
3921
1117653
25
1528
285.0
31.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.656 ± 0.045
0.62 ± 0.01
5.32 ± 0.031
8.04 ± 0.05
4.481 ± 0.026
7.267 ± 0.037
2.05 ± 0.018
6.715 ± 0.036
5.463 ± 0.042
9.896 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.682 ± 0.018
3.657 ± 0.026
3.933 ± 0.027
3.771 ± 0.025
4.892 ± 0.03
5.598 ± 0.026
5.557 ± 0.025
7.088 ± 0.036
1.062 ± 0.014
3.253 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here