Amino acid dipepetide frequency for Methylobacterium sp. (strain 4-46)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	25.638AlaAla: 25.638 ± 0.232	1.383AlaCys: 1.383 ± 0.029	6.711AlaAsp: 6.711 ± 0.068	9.422AlaGlu: 9.422 ± 0.097	4.622AlaPhe: 4.622 ± 0.055	13.829AlaGly: 13.829 ± 0.121	2.426AlaHis: 2.426 ± 0.034	5.616AlaIle: 5.616 ± 0.061	3.078AlaLys: 3.078 ± 0.053	17.375AlaLeu: 17.375 ± 0.132	3.304AlaMet: 3.304 ± 0.045	2.35AlaAsn: 2.35 ± 0.052	8.446AlaPro: 8.446 ± 0.091	4.278AlaGln: 4.278 ± 0.048	13.433AlaArg: 13.433 ± 0.123	6.599AlaSer: 6.599 ± 0.069	6.472AlaThr: 6.472 ± 0.074	10.849AlaVal: 10.849 ± 0.085	1.871AlaTrp: 1.871 ± 0.032	2.747AlaTyr: 2.747 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.136CysAla: 1.136 ± 0.027	0.102CysCys: 0.102 ± 0.006	0.487CysAsp: 0.487 ± 0.016	0.412CysGlu: 0.412 ± 0.015	0.285CysPhe: 0.285 ± 0.013	0.976CysGly: 0.976 ± 0.023	0.195CysHis: 0.195 ± 0.011	0.295CysIle: 0.295 ± 0.012	0.127CysLys: 0.127 ± 0.008	0.92CysLeu: 0.92 ± 0.019	0.126CysMet: 0.126 ± 0.007	0.168CysAsn: 0.168 ± 0.008	0.49CysPro: 0.49 ± 0.017	0.176CysGln: 0.176 ± 0.01	0.799CysArg: 0.799 ± 0.02	0.37CysSer: 0.37 ± 0.013	0.392CysThr: 0.392 ± 0.014	0.612CysVal: 0.612 ± 0.017	0.102CysTrp: 0.102 ± 0.007	0.164CysTyr: 0.164 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.327AspAla: 7.327 ± 0.061	0.383AspCys: 0.383 ± 0.014	2.443AspAsp: 2.443 ± 0.038	3.046AspGlu: 3.046 ± 0.045	1.723AspPhe: 1.723 ± 0.032	5.209AspGly: 5.209 ± 0.073	1.098AspHis: 1.098 ± 0.024	1.868AspIle: 1.868 ± 0.032	1.001AspLys: 1.001 ± 0.03	6.991AspLeu: 6.991 ± 0.056	0.746AspMet: 0.746 ± 0.018	0.801AspAsn: 0.801 ± 0.021	4.318AspPro: 4.318 ± 0.056	1.382AspGln: 1.382 ± 0.027	4.765AspArg: 4.765 ± 0.054	1.611AspSer: 1.611 ± 0.03	2.088AspThr: 2.088 ± 0.036	3.717AspVal: 3.717 ± 0.047	0.752AspTrp: 0.752 ± 0.017	1.17AspTyr: 1.17 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	10.141GluAla: 10.141 ± 0.09	0.303GluCys: 0.303 ± 0.011	2.441GluAsp: 2.441 ± 0.038	3.142GluGlu: 3.142 ± 0.046	1.352GluPhe: 1.352 ± 0.028	4.816GluGly: 4.816 ± 0.058	0.972GluHis: 0.972 ± 0.024	2.986GluIle: 2.986 ± 0.041	1.279GluLys: 1.279 ± 0.033	4.041GluLeu: 4.041 ± 0.048	1.211GluMet: 1.211 ± 0.024	1.026GluAsn: 1.026 ± 0.023	3.163GluPro: 3.163 ± 0.046	1.58GluGln: 1.58 ± 0.031	5.803GluArg: 5.803 ± 0.067	2.159GluSer: 2.159 ± 0.034	3.283GluThr: 3.283 ± 0.045	4.082GluVal: 4.082 ± 0.049	0.581GluTrp: 0.581 ± 0.018	0.723GluTyr: 0.723 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.668PheAla: 4.668 ± 0.055	0.355PheCys: 0.355 ± 0.014	2.08PheAsp: 2.08 ± 0.03	1.745PheGlu: 1.745 ± 0.03	1.141PhePhe: 1.141 ± 0.03	3.499PheGly: 3.499 ± 0.048	0.642PheHis: 0.642 ± 0.018	1.039PheIle: 1.039 ± 0.025	0.627PheLys: 0.627 ± 0.017	3.361PheLeu: 3.361 ± 0.049	0.543PheMet: 0.543 ± 0.014	0.735PheAsn: 0.735 ± 0.019	1.538PhePro: 1.538 ± 0.03	0.822PheGln: 0.822 ± 0.02	2.504PheArg: 2.504 ± 0.038	1.758PheSer: 1.758 ± 0.034	1.823PheThr: 1.823 ± 0.04	2.686PheVal: 2.686 ± 0.042	0.465PheTrp: 0.465 ± 0.016	0.767PheTyr: 0.767 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	11.974GlyAla: 11.974 ± 0.108	0.862GlyCys: 0.862 ± 0.024	4.161GlyAsp: 4.161 ± 0.06	5.135GlyGlu: 5.135 ± 0.05	3.531GlyPhe: 3.531 ± 0.051	8.263GlyGly: 8.263 ± 0.107	1.867GlyHis: 1.867 ± 0.036	4.05GlyIle: 4.05 ± 0.045	2.072GlyLys: 2.072 ± 0.039	11.076GlyLeu: 11.076 ± 0.093	1.935GlyMet: 1.935 ± 0.032	1.645GlyAsn: 1.645 ± 0.041	5.143GlyPro: 5.143 ± 0.056	2.792GlyGln: 2.792 ± 0.042	8.68GlyArg: 8.68 ± 0.076	4.699GlySer: 4.699 ± 0.084	4.871GlyThr: 4.871 ± 0.077	6.038GlyVal: 6.038 ± 0.052	1.387GlyTrp: 1.387 ± 0.029	2.136GlyTyr: 2.136 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.548HisAla: 2.548 ± 0.037	0.203HisCys: 0.203 ± 0.009	1.105HisAsp: 1.105 ± 0.022	0.919HisGlu: 0.919 ± 0.021	0.711HisPhe: 0.711 ± 0.019	1.974HisGly: 1.974 ± 0.035	0.521HisHis: 0.521 ± 0.018	0.583HisIle: 0.583 ± 0.017	0.291HisLys: 0.291 ± 0.013	2.128HisLeu: 2.128 ± 0.035	0.325HisMet: 0.325 ± 0.012	0.342HisAsn: 0.342 ± 0.014	1.486HisPro: 1.486 ± 0.029	0.482HisGln: 0.482 ± 0.017	1.722HisArg: 1.722 ± 0.032	0.715HisSer: 0.715 ± 0.02	0.658HisThr: 0.658 ± 0.019	1.413HisVal: 1.413 ± 0.026	0.322HisTrp: 0.322 ± 0.012	0.447HisTyr: 0.447 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	6.297IleAla: 6.297 ± 0.062	0.387IleCys: 0.387 ± 0.014	2.618IleAsp: 2.618 ± 0.034	2.711IleGlu: 2.711 ± 0.042	1.139IlePhe: 1.139 ± 0.024	4.352IleGly: 4.352 ± 0.054	0.702IleHis: 0.702 ± 0.02	1.27IleIle: 1.27 ± 0.03	0.87IleLys: 0.87 ± 0.022	4.033IleLeu: 4.033 ± 0.054	0.583IleMet: 0.583 ± 0.02	0.926IleAsn: 0.926 ± 0.026	2.073IlePro: 2.073 ± 0.033	0.964IleGln: 0.964 ± 0.02	3.215IleArg: 3.215 ± 0.044	1.897IleSer: 1.897 ± 0.036	2.027IleThr: 2.027 ± 0.037	3.446IleVal: 3.446 ± 0.042	0.465IleTrp: 0.465 ± 0.016	0.82IleTyr: 0.82 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.008LysAla: 3.008 ± 0.051	0.108LysCys: 0.108 ± 0.008	1.099LysAsp: 1.099 ± 0.031	1.001LysGlu: 1.001 ± 0.021	0.545LysPhe: 0.545 ± 0.016	1.879LysGly: 1.879 ± 0.035	0.371LysHis: 0.371 ± 0.014	0.95LysIle: 0.95 ± 0.026	0.572LysLys: 0.572 ± 0.02	2.053LysLeu: 2.053 ± 0.036	0.375LysMet: 0.375 ± 0.014	0.49LysAsn: 0.49 ± 0.019	1.609LysPro: 1.609 ± 0.033	0.593LysGln: 0.593 ± 0.019	1.733LysArg: 1.733 ± 0.03	1.107LysSer: 1.107 ± 0.026	1.135LysThr: 1.135 ± 0.03	1.718LysVal: 1.718 ± 0.037	0.227LysTrp: 0.227 ± 0.012	0.378LysTyr: 0.378 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	18.213LeuAla: 18.213 ± 0.153	0.988LeuCys: 0.988 ± 0.02	6.667LeuAsp: 6.667 ± 0.07	4.49LeuGlu: 4.49 ± 0.048	3.432LeuPhe: 3.432 ± 0.049	9.534LeuGly: 9.534 ± 0.086	1.914LeuHis: 1.914 ± 0.031	4.276LeuIle: 4.276 ± 0.051	2.406LeuLys: 2.406 ± 0.038	10.597LeuLeu: 10.597 ± 0.109	1.969LeuMet: 1.969 ± 0.036	2.058LeuAsn: 2.058 ± 0.035	6.505LeuPro: 6.505 ± 0.07	2.459LeuGln: 2.459 ± 0.04	9.228LeuArg: 9.228 ± 0.088	5.91LeuSer: 5.91 ± 0.058	5.814LeuThr: 5.814 ± 0.07	8.63LeuVal: 8.63 ± 0.078	1.189LeuTrp: 1.189 ± 0.026	1.933LeuTyr: 1.933 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.575MetAla: 2.575 ± 0.034	0.112MetCys: 0.112 ± 0.007	0.799MetAsp: 0.799 ± 0.02	0.785MetGlu: 0.785 ± 0.021	0.442MetPhe: 0.442 ± 0.017	1.441MetGly: 1.441 ± 0.029	0.351MetHis: 0.351 ± 0.015	0.984MetIle: 0.984 ± 0.022	0.481MetLys: 0.481 ± 0.015	1.969MetLeu: 1.969 ± 0.031	0.481MetMet: 0.481 ± 0.017	0.506MetAsn: 0.506 ± 0.017	1.367MetPro: 1.367 ± 0.028	0.603MetGln: 0.603 ± 0.017	1.917MetArg: 1.917 ± 0.031	1.323MetSer: 1.323 ± 0.027	1.483MetThr: 1.483 ± 0.027	1.291MetVal: 1.291 ± 0.023	0.144MetTrp: 0.144 ± 0.009	0.214MetTyr: 0.214 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.539AsnAla: 2.539 ± 0.037	0.176AsnCys: 0.176 ± 0.01	0.973AsnAsp: 0.973 ± 0.031	0.829AsnGlu: 0.829 ± 0.023	0.663AsnPhe: 0.663 ± 0.018	1.848AsnGly: 1.848 ± 0.042	0.355AsnHis: 0.355 ± 0.016	0.786AsnIle: 0.786 ± 0.024	0.408AsnLys: 0.408 ± 0.015	2.218AsnLeu: 2.218 ± 0.034	0.345AsnMet: 0.345 ± 0.012	0.455AsnAsn: 0.455 ± 0.016	1.532AsnPro: 1.532 ± 0.03	0.578AsnGln: 0.578 ± 0.021	1.577AsnArg: 1.577 ± 0.027	0.792AsnSer: 0.792 ± 0.029	0.901AsnThr: 0.901 ± 0.024	1.446AsnVal: 1.446 ± 0.027	0.279AsnTrp: 0.279 ± 0.013	0.468AsnTyr: 0.468 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.437ProAla: 9.437 ± 0.106	0.385ProCys: 0.385 ± 0.015	4.246ProAsp: 4.246 ± 0.052	4.275ProGlu: 4.275 ± 0.05	2.013ProPhe: 2.013 ± 0.033	6.173ProGly: 6.173 ± 0.068	1.167ProHis: 1.167 ± 0.024	2.095ProIle: 2.095 ± 0.036	1.248ProLys: 1.248 ± 0.029	5.65ProLeu: 5.65 ± 0.06	1.141ProMet: 1.141 ± 0.022	1.141ProAsn: 1.141 ± 0.024	4.039ProPro: 4.039 ± 0.062	1.477ProGln: 1.477 ± 0.028	4.635ProArg: 4.635 ± 0.061	2.981ProSer: 2.981 ± 0.04	2.538ProThr: 2.538 ± 0.036	4.882ProVal: 4.882 ± 0.051	0.759ProTrp: 0.759 ± 0.02	1.116ProTyr: 1.116 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.354GlnAla: 4.354 ± 0.06	0.167GlnCys: 0.167 ± 0.009	1.432GlnAsp: 1.432 ± 0.032	1.366GlnGlu: 1.366 ± 0.031	0.824GlnPhe: 0.824 ± 0.022	2.381GlnGly: 2.381 ± 0.039	0.517GlnHis: 0.517 ± 0.016	1.415GlnIle: 1.415 ± 0.028	0.639GlnLys: 0.639 ± 0.018	2.354GlnLeu: 2.354 ± 0.04	0.546GlnMet: 0.546 ± 0.016	0.636GlnAsn: 0.636 ± 0.023	1.521GlnPro: 1.521 ± 0.03	0.915GlnGln: 0.915 ± 0.028	2.22GlnArg: 2.22 ± 0.035	1.319GlnSer: 1.319 ± 0.023	1.365GlnThr: 1.365 ± 0.024	2.322GlnVal: 2.322 ± 0.035	0.289GlnTrp: 0.289 ± 0.012	0.47GlnTyr: 0.47 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	12.539ArgAla: 12.539 ± 0.115	0.618ArgCys: 0.618 ± 0.017	4.96ArgAsp: 4.96 ± 0.054	5.11ArgGlu: 5.11 ± 0.059	3.124ArgPhe: 3.124 ± 0.04	6.624ArgGly: 6.624 ± 0.06	1.987ArgHis: 1.987 ± 0.037	4.039ArgIle: 4.039 ± 0.045	1.642ArgLys: 1.642 ± 0.028	10.258ArgLeu: 10.258 ± 0.106	1.794ArgMet: 1.794 ± 0.028	1.533ArgAsn: 1.533 ± 0.031	5.67ArgPro: 5.67 ± 0.068	2.51ArgGln: 2.51 ± 0.037	8.712ArgArg: 8.712 ± 0.091	3.877ArgSer: 3.877 ± 0.047	3.873ArgThr: 3.873 ± 0.047	6.157ArgVal: 6.157 ± 0.064	1.081ArgTrp: 1.081 ± 0.023	1.679ArgTyr: 1.679 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.071SerAla: 6.071 ± 0.069	0.41SerCys: 0.41 ± 0.014	2.462SerAsp: 2.462 ± 0.042	2.364SerGlu: 2.364 ± 0.033	1.936SerPhe: 1.936 ± 0.036	5.259SerGly: 5.259 ± 0.086	0.911SerHis: 0.911 ± 0.02	1.826SerIle: 1.826 ± 0.035	0.952SerLys: 0.952 ± 0.027	5.525SerLeu: 5.525 ± 0.054	0.858SerMet: 0.858 ± 0.019	0.924SerAsn: 0.924 ± 0.021	3.032SerPro: 3.032 ± 0.036	1.235SerGln: 1.235 ± 0.025	3.793SerArg: 3.793 ± 0.047	2.273SerSer: 2.273 ± 0.039	2.198SerThr: 2.198 ± 0.035	3.563SerVal: 3.563 ± 0.047	0.645SerTrp: 0.645 ± 0.018	1.134SerTyr: 1.134 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	6.489ThrAla: 6.489 ± 0.07	0.429ThrCys: 0.429 ± 0.014	2.365ThrAsp: 2.365 ± 0.039	2.26ThrGlu: 2.26 ± 0.035	1.731ThrPhe: 1.731 ± 0.031	5.266ThrGly: 5.266 ± 0.086	0.9ThrHis: 0.9 ± 0.02	2.291ThrIle: 2.291 ± 0.044	0.988ThrLys: 0.988 ± 0.036	5.866ThrLeu: 5.866 ± 0.06	0.902ThrMet: 0.902 ± 0.023	1.021ThrAsn: 1.021 ± 0.027	3.184ThrPro: 3.184 ± 0.046	1.193ThrGln: 1.193 ± 0.026	3.828ThrArg: 3.828 ± 0.046	2.337ThrSer: 2.337 ± 0.039	2.494ThrThr: 2.494 ± 0.049	4.348ThrVal: 4.348 ± 0.06	0.669ThrTrp: 0.669 ± 0.019	1.194ThrTyr: 1.194 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	11.44ValAla: 11.44 ± 0.086	0.614ValCys: 0.614 ± 0.018	3.6ValAsp: 3.6 ± 0.041	4.57ValGlu: 4.57 ± 0.054	2.384ValPhe: 2.384 ± 0.036	5.978ValGly: 5.978 ± 0.067	1.312ValHis: 1.312 ± 0.024	3.236ValIle: 3.236 ± 0.048	1.553ValLys: 1.553 ± 0.034	8.078ValLeu: 8.078 ± 0.073	1.526ValMet: 1.526 ± 0.029	1.607ValAsn: 1.607 ± 0.034	4.55ValPro: 4.55 ± 0.047	2.052ValGln: 2.052 ± 0.035	6.008ValArg: 6.008 ± 0.056	3.981ValSer: 3.981 ± 0.053	4.661ValThr: 4.661 ± 0.07	6.355ValVal: 6.355 ± 0.066	0.842ValTrp: 0.842 ± 0.018	1.336ValTyr: 1.336 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.258TrpAla: 1.258 ± 0.025	0.145TrpCys: 0.145 ± 0.008	0.585TrpAsp: 0.585 ± 0.018	0.481TrpGlu: 0.481 ± 0.014	0.501TrpPhe: 0.501 ± 0.016	0.897TrpGly: 0.897 ± 0.022	0.304TrpHis: 0.304 ± 0.014	0.596TrpIle: 0.596 ± 0.019	0.311TrpLys: 0.311 ± 0.013	1.574TrpLeu: 1.574 ± 0.03	0.268TrpMet: 0.268 ± 0.012	0.338TrpAsn: 0.338 ± 0.013	0.727TrpPro: 0.727 ± 0.019	0.449TrpGln: 0.449 ± 0.016	1.355TrpArg: 1.355 ± 0.028	0.839TrpSer: 0.839 ± 0.018	0.792TrpThr: 0.792 ± 0.02	0.692TrpVal: 0.692 ± 0.02	0.208TrpTrp: 0.208 ± 0.01	0.275TrpTyr: 0.275 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.794TyrAla: 2.794 ± 0.053	0.204TyrCys: 0.204 ± 0.01	1.205TyrAsp: 1.205 ± 0.032	1.021TyrGlu: 1.021 ± 0.023	0.701TyrPhe: 0.701 ± 0.017	2.118TyrGly: 2.118 ± 0.04	0.384TyrHis: 0.384 ± 0.013	0.595TyrIle: 0.595 ± 0.018	0.403TyrLys: 0.403 ± 0.014	2.147TyrLeu: 2.147 ± 0.036	0.291TyrMet: 0.291 ± 0.011	0.468TyrAsn: 0.468 ± 0.019	1.005TyrPro: 1.005 ± 0.024	0.513TyrGln: 0.513 ± 0.015	1.858TyrArg: 1.858 ± 0.035	0.824TyrSer: 0.824 ± 0.025	0.886TyrThr: 0.886 ± 0.028	1.436TyrVal: 1.436 ± 0.028	0.316TyrTrp: 0.316 ± 0.012	0.483TyrTyr: 0.483 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6647 proteins (2076946 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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