Thiomonas intermedia (strain K12) (Thiobacillus intermedius)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Thiomonas; Thiomonas intermedia

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5X312|D5X312_THIK1 Malic protein NAD-binding protein OS=Thiomonas intermedia (strain K12) OX=75379 GN=Tint_0097 PE=3 SV=1
MM1 pKa = 7.4PVSPIRR7 pKa = 11.84LSLGAALLASLALAGCGGGATNTNYY32 pKa = 10.52SGSTVTGQVLMGANSPVNGNGTNNVCAYY60 pKa = 10.39AIVNGQGNPLTTTVTPYY77 pKa = 10.09TSYY80 pKa = 9.73GTVLDD85 pKa = 4.76CVPSAADD92 pKa = 3.95GSFSMNLTSFYY103 pKa = 11.07GPVLLQVVGGTYY115 pKa = 10.07SYY117 pKa = 11.54NGASQALNSLTSTALNLAASASNAALATNASLQAMVNVGGGGTVTANITPLTTFAVARR175 pKa = 11.84ITPNSGLTLANYY187 pKa = 8.9SASLQDD193 pKa = 3.14IAGQFGLGGLALATAVPTAGDD214 pKa = 3.99AYY216 pKa = 10.53DD217 pKa = 3.6KK218 pKa = 11.49ALIGVDD224 pKa = 3.53QYY226 pKa = 11.59LLNMGTTLGATNDD239 pKa = 3.77PYY241 pKa = 11.46GANLLNWTNLPTVSADD257 pKa = 3.07YY258 pKa = 9.63STAYY262 pKa = 9.99NQANGTTGVSFSFNN276 pKa = 3.22

Molecular weight: 27.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5X0B2|D5X0B2_THIK1 Uncharacterized protein OS=Thiomonas intermedia (strain K12) OX=75379 GN=Tint_1168 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.53TRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3134

0

3134

1016059

32

2469

324.2

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.976 ± 0.063

0.895 ± 0.014

5.127 ± 0.032

4.821 ± 0.041

3.438 ± 0.027

7.991 ± 0.042

2.393 ± 0.023

4.375 ± 0.032

3.026 ± 0.031

11.374 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.02

2.559 ± 0.026

5.529 ± 0.032

4.85 ± 0.038

6.811 ± 0.05

5.352 ± 0.036

5.125 ± 0.031

7.222 ± 0.037

1.553 ± 0.019

2.225 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski