Odocoileus virginianus papillomavirus 1 (DPV) (Deer papillomavirus)
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P03117|VE1_OVPVD Replication protein E1 OS=Odocoileus virginianus papillomavirus 1 OX=2772504 GN=E1 PE=3 SV=2
MM1 pKa = 7.74 PPLKK5 pKa = 9.91 RR6 pKa = 11.84 VKK8 pKa = 10.17 RR9 pKa = 11.84 ANPYY13 pKa = 10.57 DD14 pKa = 3.99 LYY16 pKa = 10.14 RR17 pKa = 11.84 TCKK20 pKa = 9.47 RR21 pKa = 11.84 WKK23 pKa = 9.5 CPLMSFLKK31 pKa = 10.98 VEE33 pKa = 4.52 GKK35 pKa = 8.16 TVADD39 pKa = 5.18 KK40 pKa = 9.86 YY41 pKa = 11.18 CSMGSMGVYY50 pKa = 8.77 WRR52 pKa = 11.84 LALHH56 pKa = 6.52 GSGRR60 pKa = 11.84 PTQGGYY66 pKa = 10.17 VPLRR70 pKa = 11.84 GGGSSTSLSSRR81 pKa = 11.84 GSGSSTSISRR91 pKa = 11.84 PFAGGIPLEE100 pKa = 4.18 TLEE103 pKa = 4.42 TVGAFRR109 pKa = 11.84 PGIIEE114 pKa = 3.95 EE115 pKa = 4.24 VAPTLEE121 pKa = 4.42 GVLPDD126 pKa = 3.8 APAVVTPEE134 pKa = 4.29 AVPVDD139 pKa = 3.71 QGLSGLDD146 pKa = 3.2 VARR149 pKa = 11.84 EE150 pKa = 4.1 VTQEE154 pKa = 3.85 SLITFLQPEE163 pKa = 4.94 GPDD166 pKa = 3.93 DD167 pKa = 3.69 IAVLEE172 pKa = 4.23 LRR174 pKa = 11.84 PTEE177 pKa = 4.11 HH178 pKa = 7.18 DD179 pKa = 3.34 QTHH182 pKa = 7.13 LISTSTHH189 pKa = 6.26 PNPLFHH195 pKa = 7.52 APIQQSSIIAEE206 pKa = 4.2 TSGSEE211 pKa = 4.03 NIFVGGGGVGSTTGEE226 pKa = 4.18 EE227 pKa = 3.98 IEE229 pKa = 4.48 LTLFGQPKK237 pKa = 8.73 TSTPEE242 pKa = 3.79 GPINRR247 pKa = 11.84 GRR249 pKa = 11.84 GIFNWFNRR257 pKa = 11.84 TYY259 pKa = 8.2 YY260 pKa = 8.89 TQVPVEE266 pKa = 4.88 DD267 pKa = 4.44 PDD269 pKa = 4.14 EE270 pKa = 4.1 IAAAGSYY277 pKa = 9.78 VFEE280 pKa = 4.31 NALYY284 pKa = 10.27 DD285 pKa = 3.81 SKK287 pKa = 11.41 AYY289 pKa = 9.72 KK290 pKa = 10.18 HH291 pKa = 6.08 EE292 pKa = 3.99 QQPWLSRR299 pKa = 11.84 PQDD302 pKa = 3.38 APEE305 pKa = 4.62 FDD307 pKa = 3.81 FQDD310 pKa = 3.69 AVRR313 pKa = 11.84 LLQGPSGRR321 pKa = 11.84 VGWSRR326 pKa = 11.84 IIRR329 pKa = 11.84 PTSIGTRR336 pKa = 11.84 SGVRR340 pKa = 11.84 VGPLYY345 pKa = 10.53 HH346 pKa = 6.78 LRR348 pKa = 11.84 QSFSTIDD355 pKa = 3.46 EE356 pKa = 4.63 PEE358 pKa = 4.32 TIEE361 pKa = 5.52 LIPSTVDD368 pKa = 3.0 EE369 pKa = 4.78 EE370 pKa = 4.5 EE371 pKa = 4.36 VLTGVPEE378 pKa = 4.27 SAEE381 pKa = 4.24 GPDD384 pKa = 4.57 AEE386 pKa = 4.87 YY387 pKa = 11.31 SDD389 pKa = 5.7 IDD391 pKa = 3.89 LQSIGSDD398 pKa = 3.35 EE399 pKa = 4.36 PLLGTGIIYY408 pKa = 9.81 PLVGGGQIFLCMHH421 pKa = 7.58 RR422 pKa = 11.84 APVGWSSGTYY432 pKa = 9.52 INHH435 pKa = 6.77 EE436 pKa = 4.14 GQSRR440 pKa = 11.84 DD441 pKa = 3.37 DD442 pKa = 3.7 GEE444 pKa = 4.34 YY445 pKa = 10.93 VIDD448 pKa = 5.49 NGGQSNITPTVVIDD462 pKa = 3.74 GSIALSLEE470 pKa = 4.25 YY471 pKa = 10.11 FRR473 pKa = 11.84 HH474 pKa = 6.0 YY475 pKa = 10.47 YY476 pKa = 10.03 LHH478 pKa = 7.25 PSLLRR483 pKa = 11.84 RR484 pKa = 11.84 KK485 pKa = 9.7 RR486 pKa = 11.84 KK487 pKa = 9.64 RR488 pKa = 11.84 NPIFII493 pKa = 4.84
Molecular weight: 53.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.999
IPC2_protein 5.041
IPC_protein 5.003
Toseland 4.952
ProMoST 5.13
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.851
Rodwell 4.914
Grimsley 4.876
Solomon 4.991
Lehninger 4.94
Nozaki 5.105
DTASelect 5.245
Thurlkill 4.952
EMBOSS 4.889
Sillero 5.181
Patrickios 3.923
IPC_peptide 4.991
IPC2_peptide 5.181
IPC2.peptide.svr19 5.134
Protein with the highest isoelectric point:
>sp|P06925|VE4_OVPVD Probable protein E4 OS=Odocoileus virginianus papillomavirus 1 OX=2772504 GN=E4 PE=4 SV=1
GG1 pKa = 5.97 ITIVLITHH9 pKa = 6.88 ILRR12 pKa = 11.84 PPTLEE17 pKa = 4.86 RR18 pKa = 11.84 PSKK21 pKa = 11.04 DD22 pKa = 3.0 CGTPGAVKK30 pKa = 10.12 EE31 pKa = 4.38 ADD33 pKa = 3.46 PPTRR37 pKa = 11.84 PTAPCFTLLLEE48 pKa = 4.5 ATPFTVPSEE57 pKa = 3.98 LAKK60 pKa = 10.34 TGVGPLTARR69 pKa = 11.84 LPTAHH74 pKa = 7.03 HH75 pKa = 6.43 SPRR78 pKa = 11.84 GVAWAPIPPPRR89 pKa = 11.84 CRR91 pKa = 11.84 ARR93 pKa = 11.84 YY94 pKa = 9.02 RR95 pKa = 11.84 RR96 pKa = 11.84 TPGAYY101 pKa = 10.25 LYY103 pKa = 7.52 PTVLDD108 pKa = 3.46 EE109 pKa = 4.21 GRR111 pKa = 11.84 RR112 pKa = 11.84 ITRR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 QINTTT122 pKa = 3.32
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.736
IPC_protein 10.891
Toseland 10.862
ProMoST 10.95
Dawson 10.935
Bjellqvist 10.789
Wikipedia 11.272
Rodwell 10.877
Grimsley 10.994
Solomon 11.213
Lehninger 11.14
Nozaki 10.877
DTASelect 10.789
Thurlkill 10.862
EMBOSS 11.301
Sillero 10.891
Patrickios 10.628
IPC_peptide 11.213
IPC2_peptide 10.204
IPC2.peptide.svr19 8.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2429
35
613
303.6
33.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.175 ± 0.692
2.758 ± 0.744
5.105 ± 0.297
6.093 ± 0.318
3.541 ± 0.469
7.452 ± 0.876
2.594 ± 0.297
4.199 ± 0.62
4.446 ± 0.624
8.687 ± 0.383
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.811 ± 0.354
3.746 ± 0.571
7.369 ± 1.13
3.788 ± 0.262
6.175 ± 0.893
7.657 ± 0.706
7.122 ± 0.652
6.093 ± 0.303
1.688 ± 0.315
3.499 ± 0.5
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here