Sporocytophaga myxococcoides
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5042 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098LE06|A0A098LE06_9BACT Putative phosphoesterase OS=Sporocytophaga myxococcoides OX=153721 GN=MYP_1556 PE=4 SV=1
MM1 pKa = 7.55 LMKK4 pKa = 10.54 YY5 pKa = 10.33 LLGLIIVLGLGFNSFAQTPQDD26 pKa = 3.77 EE27 pKa = 4.41 QNEE30 pKa = 4.33 DD31 pKa = 3.46 SFICYY36 pKa = 9.51 QVDD39 pKa = 3.67 EE40 pKa = 4.85 NSISCYY46 pKa = 10.2 SDD48 pKa = 3.2 YY49 pKa = 11.56 DD50 pKa = 3.67 PAFDD54 pKa = 3.46 EE55 pKa = 4.64 TFQQGVTPEE64 pKa = 3.71 GDD66 pKa = 2.83 IYY68 pKa = 11.62 SMEE71 pKa = 4.17 EE72 pKa = 3.98 NIIEE76 pKa = 4.79 SPGQEE81 pKa = 3.67 EE82 pKa = 4.07 NASEE86 pKa = 3.98 EE87 pKa = 4.49 FYY89 pKa = 10.71 RR90 pKa = 11.84 IEE92 pKa = 4.1 PLEE95 pKa = 4.18 LEE97 pKa = 4.29 GTPQCEE103 pKa = 4.02 ANEE106 pKa = 4.1 KK107 pKa = 10.58 EE108 pKa = 3.96 EE109 pKa = 3.95 AKK111 pKa = 10.53 AYY113 pKa = 9.09 NRR115 pKa = 11.84 PEE117 pKa = 3.88 NYY119 pKa = 9.43 EE120 pKa = 4.07 DD121 pKa = 4.83 KK122 pKa = 11.09 SNEE125 pKa = 4.38 DD126 pKa = 2.84 ISQIGDD132 pKa = 3.28 QEE134 pKa = 4.4 RR135 pKa = 11.84 GEE137 pKa = 4.08 NTVNSEE143 pKa = 3.53 ICSQLEE149 pKa = 3.9 RR150 pKa = 11.84 SDD152 pKa = 3.35 GKK154 pKa = 11.33 SFGEE158 pKa = 4.18 PYY160 pKa = 10.71 DD161 pKa = 3.76 FEE163 pKa = 5.07 KK164 pKa = 10.69 AQCYY168 pKa = 9.24 CPDD171 pKa = 3.67 MEE173 pKa = 4.63 TTTEE177 pKa = 3.78 GDD179 pKa = 3.9 YY180 pKa = 11.42 KK181 pKa = 10.82 FGNPDD186 pKa = 3.28 EE187 pKa = 5.11 EE188 pKa = 4.52 INPEE192 pKa = 4.01 DD193 pKa = 3.46 VHH195 pKa = 8.9 IEE197 pKa = 4.39 TNVSTDD203 pKa = 2.63 EE204 pKa = 4.3 GGIYY208 pKa = 10.37
Molecular weight: 23.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.49
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.516
Rodwell 3.516
Grimsley 3.401
Solomon 3.63
Lehninger 3.579
Nozaki 3.745
DTASelect 3.884
Thurlkill 3.528
EMBOSS 3.528
Sillero 3.795
Patrickios 0.337
IPC_peptide 3.63
IPC2_peptide 3.77
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|A0A098LJG5|A0A098LJG5_9BACT ATE_C domain-containing protein OS=Sporocytophaga myxococcoides OX=153721 GN=MYP_3854 PE=4 SV=1
MM1 pKa = 7.41 GRR3 pKa = 11.84 TFLRR7 pKa = 11.84 IVFIRR12 pKa = 11.84 AHH14 pKa = 6.31 FKK16 pKa = 10.7 LPTRR20 pKa = 11.84 HH21 pKa = 6.6 PYY23 pKa = 8.53 HH24 pKa = 6.92 ARR26 pKa = 11.84 MWFIINQIRR35 pKa = 11.84 FIRR38 pKa = 11.84 ANSNIFHH45 pKa = 7.0 RR46 pKa = 11.84 HH47 pKa = 4.08 AQLL50 pKa = 3.62
Molecular weight: 6.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 10.965
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.047
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5042
0
5042
1718912
50
5647
340.9
38.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.149 ± 0.034
0.904 ± 0.014
5.271 ± 0.021
6.393 ± 0.043
5.206 ± 0.03
6.704 ± 0.037
1.668 ± 0.015
8.058 ± 0.038
7.891 ± 0.042
9.227 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.058 ± 0.014
5.999 ± 0.034
3.653 ± 0.025
3.123 ± 0.019
3.539 ± 0.028
7.175 ± 0.034
5.553 ± 0.049
6.122 ± 0.029
1.063 ± 0.011
4.243 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here