Microbacterium aurum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8U5L6|A0A1P8U5L6_9MICO Uncharacterized protein OS=Microbacterium aurum OX=36805 GN=BOH66_03165 PE=4 SV=1
MM1 pKa = 7.3RR2 pKa = 11.84TKK4 pKa = 10.43KK5 pKa = 10.7VLGITAGLAAALLVAGCSGGGGSGDD30 pKa = 3.48GDD32 pKa = 3.74GSQDD36 pKa = 3.26QHH38 pKa = 4.68VTLWLYY44 pKa = 10.22PILADD49 pKa = 3.74EE50 pKa = 4.98AAHH53 pKa = 6.41KK54 pKa = 10.18AHH56 pKa = 7.35WDD58 pKa = 3.41QVVEE62 pKa = 5.05DD63 pKa = 4.84FTADD67 pKa = 3.34HH68 pKa = 7.16PEE70 pKa = 3.58ITVDD74 pKa = 3.8YY75 pKa = 10.82EE76 pKa = 3.71IFPWANRR83 pKa = 11.84DD84 pKa = 3.28EE85 pKa = 4.47ALQTAIAAKK94 pKa = 9.39TGPDD98 pKa = 3.73LVYY101 pKa = 10.71LIPDD105 pKa = 3.25QLAAYY110 pKa = 9.34QDD112 pKa = 4.33AIEE115 pKa = 5.13PIGPYY120 pKa = 10.5LSDD123 pKa = 3.4EE124 pKa = 4.4RR125 pKa = 11.84KK126 pKa = 9.92SDD128 pKa = 3.66LLDD131 pKa = 3.41NVMEE135 pKa = 4.42SVTLDD140 pKa = 3.69GEE142 pKa = 4.77LMGAPMLTSALPLLCDD158 pKa = 3.61GQVFEE163 pKa = 5.09AAGLTEE169 pKa = 5.18YY170 pKa = 10.65PEE172 pKa = 4.04TWDD175 pKa = 4.17DD176 pKa = 3.79VLEE179 pKa = 4.35IAPQLSEE186 pKa = 3.85QGLYY190 pKa = 9.12TISYY194 pKa = 8.51SAYY197 pKa = 9.81PEE199 pKa = 3.85MTLNQSFYY207 pKa = 10.61PLLWQAGGSIYY218 pKa = 10.71SEE220 pKa = 4.99DD221 pKa = 3.73GSSVGFNEE229 pKa = 4.21EE230 pKa = 4.11PGVEE234 pKa = 3.73ALSFLTEE241 pKa = 4.02LAEE244 pKa = 4.15QDD246 pKa = 3.91ALVPDD251 pKa = 4.71ALTTNVPMEE260 pKa = 3.97QTAIAQHH267 pKa = 6.11KK268 pKa = 7.38VACTWNHH275 pKa = 5.09SVAEE279 pKa = 4.2VQPFWDD285 pKa = 5.71DD286 pKa = 3.06IHH288 pKa = 8.27VVAPLKK294 pKa = 10.78DD295 pKa = 3.86KK296 pKa = 11.19EE297 pKa = 4.4SVAYY301 pKa = 8.31GTVGSLAMLTGAQDD315 pKa = 3.5KK316 pKa = 9.76EE317 pKa = 3.87AAAAFAEE324 pKa = 4.7YY325 pKa = 8.62ATGEE329 pKa = 4.37DD330 pKa = 3.72VVVPYY335 pKa = 8.77LTQAGFFSALKK346 pKa = 9.42STGEE350 pKa = 4.43LYY352 pKa = 10.94ADD354 pKa = 4.52DD355 pKa = 4.85PVLSAVEE362 pKa = 3.88ATIPSTTVGEE372 pKa = 4.58LNTSSRR378 pKa = 11.84EE379 pKa = 4.0LMGVLVPEE387 pKa = 4.43IQAALLGQKK396 pKa = 9.37SAQEE400 pKa = 4.17ALDD403 pKa = 3.76AAAAAAEE410 pKa = 4.06PLLAKK415 pKa = 10.48

Molecular weight: 44.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8U606|A0A1P8U606_9MICO ATPase OS=Microbacterium aurum OX=36805 GN=BOH66_03925 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

966754

29

3126

316.6

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.259 ± 0.077

0.527 ± 0.011

6.39 ± 0.04

5.478 ± 0.041

3.002 ± 0.029

8.768 ± 0.038

2.035 ± 0.023

4.466 ± 0.032

1.923 ± 0.035

10.004 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.018

1.821 ± 0.022

5.409 ± 0.029

2.858 ± 0.024

7.591 ± 0.054

5.177 ± 0.03

6.134 ± 0.042

8.928 ± 0.041

1.514 ± 0.021

1.977 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski